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The map label for this gene is narX [H]

Identifier: 194737257

GI number: 194737257

Start: 1840307

End: 1842103

Strand: Direct

Name: narX [H]

Synonym: SeSA_A1902

Alternate gene names: 194737257

Gene position: 1840307-1842103 (Clockwise)

Preceding gene: 194734046

Following gene: 194736641

Centisome position: 39.08

GC content: 53.92

Gene sequence:

>1797_bases
ATGTTTAAACGGTGTTTCTCTCCTCTTACGCTGGTTAACCAATTGGCGCTGATTGTCATGCTCTCTACCGCTATCGGCGT
GGCGGGGATGGCGGTTTCCGGCTGGCTTGTTCAGGGCGTACAGGGTAGCGCTCATGCGATTAATAAAGCGGGCTCATTGC
GTATGCAAAGCTATCGACTACTGGCCGCTGTCCCGCTGGATGCTAAAGATCAAAAGCTGCTGGATGAGATGGAGCAAACG
GCATTCAGCCCGGAACTGACCCGCGCCGCTGAGCGAGACGGGCAGCAGAAGCAGCTTAAAGCGTTGCAGGATTACTGGCA
TAACGAACTCTCGCCAGGGCTACAGCACGCGCAAAACGCGCACGCGGTCGCAGAAGATGTCACGCGCTTTGTAGCGGGCC
TGGATCGCCTGGTGACGTCGTTCGACCATACTACTGAATTGCGTATCGAACGCGTGGTATTAGTTCACCGGGTAATGGCG
ATCTTTATGGCGCTATTGCTGGTATTTACGATTATCTGGCTACGTGTTCGCCTTTTGCAGCCCTGGAAACAGTTATTATC
AATGGCGCGCGCCGTCAGTCAGCGCGACTTTACCCAACGTGCCAATATCAGTGGACGCAATGAGATGGCCGCGTTGGGTT
CTGCGCTCAATAATATGTCTGAAGAACTGGCCGAAAGTTATGCGGTACTGGAACAGCGCGTACAGGAAAAAACAGCCGGT
CTGGAACATAAAAACCAGATTCTCTCGTTTTTGTGGCAAGCCAACCGTCGGCTGCATTCACCGGCTCCGCTATGTGAGCG
TCTTTCACCGGTACTGAATGGCCTGCAAAATTTAACCCAGTTGCATGATATTGAGCTGCGAGTCTATGACCTGGAAGATG
AAGATAATCACCAGGAATTTACCTGTCAGTCGGACATCAGTTGCGATGATAAAGGCTGCCACCTTTGCCCACGTAGCGCC
CTGCCGATGATTAACGGCGGCACCACTTTAAAATGGCGGCTCACCGATTCTCACACCCAATACGGTATTTTGCTGGCGAC
GCTGCCCTATGGACGCCATCTCAGCCACGATCAGCAACAGTTGGTGGACACGCTGGTTGAACAGCTTACCGCGACTCTGG
CGCTCGACAGGCATCAGGAGCGCCAGCAGCAGTTGATTGTCATGGAAGAGCGTGCCACCATTGCCCGCGAACTGCACGAT
TCTATTGCCCAGTCGCTCTCTTGTATGAAGATGCAGGTGAGCTGCCTGCAAATGCAGGGCGACGTGTTACCGGAAAGCAG
CCGTGTGTTACTCAGCCAAATTCGCAATGAGCTGAACTGCTCCTGGGCGCAACTGCGCGAACTGTTAACCACTTTCCGGT
TACAGTTGACGGAACCCGGGCTACGTCCGGCGCTGGAGGCAAGCTGCCAGGAGTTCAGCGCCCGGTTTGGCTTTACGGTA
AAACTGGATTATCAGCTTCCCCCCCGCCTGGTGCCTTCACACCAGGCGATACACCTGTTGCAAATTGCCAGGGAAGCCCT
CAGCAACGCCCTTAAACACTCTCATGCCGACGATGTGGTGGTGACAGTGACGCAATGTGGCAAACAGGTCAAACTAAAAG
TGCAGGATAATGGCTGCGGCGTCCCGGAAAACGCCGAACGCAGTAATCACTACGGCATGATCATCATGCGCGATCGGGCG
CAAAGTTTGCGCGGCGATTGCCAGGTACGCCGACGCGAGACTGGCGGAACAGAGGTCACTGTTACTTTTATTCCAGAAAC
AAACTTCACAGAAACCCAGGGAGATACCCATGAATAA

Upstream 100 bases:

>100_bases
GGTATACTCCGCTGTATCGTTGAGAATAGCGGCACAGCCGATTGACGCCCCTTTTTTGCTACGTTTTTTCGGCGACATTA
CCCCGAAGAAAGAAGGTAAC

Downstream 100 bases:

>100_bases
TCAGGAACCGGCAACCATCCTGTTAATTGACGACCATCCGATGCTACGCACGGGTGTAAAGCAGCTTGTGAGTATGGCGC
CCGATATCAGCGTGGTCGGA

Product: nitrate/nitrite sensor protein NarX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLDAKDQKLLDEMEQT
AFSPELTRAAERDGQQKQLKALQDYWHNELSPGLQHAQNAHAVAEDVTRFVAGLDRLVTSFDHTTELRIERVVLVHRVMA
IFMALLLVFTIIWLRVRLLQPWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSPAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEFTCQSDISCDDKGCHLCPRSA
LPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDVLPESSRVLLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCGVPENAERSNHYGMIIMRDRA
QSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE

Sequences:

>Translated_598_residues
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLDAKDQKLLDEMEQT
AFSPELTRAAERDGQQKQLKALQDYWHNELSPGLQHAQNAHAVAEDVTRFVAGLDRLVTSFDHTTELRIERVVLVHRVMA
IFMALLLVFTIIWLRVRLLQPWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSPAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEFTCQSDISCDDKGCHLCPRSA
LPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDVLPESSRVLLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCGVPENAERSNHYGMIIMRDRA
QSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE
>Mature_598_residues
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLDAKDQKLLDEMEQT
AFSPELTRAAERDGQQKQLKALQDYWHNELSPGLQHAQNAHAVAEDVTRFVAGLDRLVTSFDHTTELRIERVVLVHRVMA
IFMALLLVFTIIWLRVRLLQPWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSPAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEFTCQSDISCDDKGCHLCPRSA
LPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDVLPESSRVLLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCGVPENAERSNHYGMIIMRDRA
QSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=598, Percent_Identity=85.9531772575251, Blast_Score=1058, Evalue=0.0,
Organism=Escherichia coli, GI1788812, Length=586, Percent_Identity=30.0341296928328, Blast_Score=193, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67378; Mature: 67378

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHE
LAAVPLDAKDQKLLDEMEQTAFSPELTRAAERDGQQKQLKALQDYWHNELSPGLQHAQNA
EEECCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
HAVAEDVTRFVAGLDRLVTSFDHTTELRIERVVLVHRVMAIFMALLLVFTIIWLRVRLLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEHKNQILSFLWQANRRLHSPAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEF
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCE
TCQSDISCDDKGCHLCPRSALPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQ
EECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHHH
LVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DVLPESSRVLLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEE
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCG
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCEEEEEECCCCCC
VPENAERSNHYGMIIMRDRAQSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE
CCCCCCCCCCCCEEEECCCHHHHHCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHE
LAAVPLDAKDQKLLDEMEQTAFSPELTRAAERDGQQKQLKALQDYWHNELSPGLQHAQNA
EEECCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
HAVAEDVTRFVAGLDRLVTSFDHTTELRIERVVLVHRVMAIFMALLLVFTIIWLRVRLLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEHKNQILSFLWQANRRLHSPAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEF
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCE
TCQSDISCDDKGCHLCPRSALPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQ
EECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHHH
LVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DVLPESSRVLLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEE
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCG
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCEEEEEECCCCCC
VPENAERSNHYGMIIMRDRAQSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE
CCCCCCCCCCCCEEEECCCHHHHHCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]