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The map label for this gene is hpaI [H]

Identifier: 194736536

GI number: 194736536

Start: 1151845

End: 1152636

Strand: Direct

Name: hpaI [H]

Synonym: SeSA_A1170

Alternate gene names: 194736536

Gene position: 1151845-1152636 (Clockwise)

Preceding gene: 194735094

Following gene: 194736359

Centisome position: 24.46

GC content: 60.48

Gene sequence:

>792_bases
ATGAAAAATGCTTTCAAAGACGCGTTAAAAGTGGGGCGTCCGCAAATCGGTTTGTGGCTGGGGCTTGCCAACAGTTACAG
CGCTGAACTGTTAGCGGGCTCCGGCTTCGACTGGCTACTGATTGACGGTGAACACGCGCCAAACAATGTGCAGACGGTGT
TGACCCAGTTGCAGGCGATTGCGCCTTATCCCAGCCAGCCGGTGGTGCGTCCGTCATGGAACGATCCGGTACAGATTAAG
CAACTGCTCGACGTCGGCGCGCAAACGCTGCTGATACCGATGGTGCAGAATGCCGATGAAGCGCGAAACGCCGTGGCGGC
TACGCGTTATCCGCCTGCCGGTATTCGCGGCGTGGGCAGCGCGCTGGCGCGGGCATCGCGCTGGAATCGCATTCCGGACT
ATCTCCACCAGGCCAACGACGCCATGTGCGTACTGGTGCAGATTGAAACGCGTGAGGCGATGAGCAATCTGGCGTCAATT
CTCGACGTGGATGGCATTGATGGCGTGTTTATTGGCCCGGCGGATCTCAGCGCCGATATGGGCTTTGCCGGCAATCCGCA
GCACCCGGAAGTGCAGGCGGCGATTGAGAACGCCATCGTGCAGATACGCGCGGCGGGGAAAGCGCCGGGGATTCTGATGG
CCAATGAAGCACTGGCGAAACGTTATCTGGAACTGGGGGCGCTATTTGTCGCCGTCGGCGTTGACACCACGCTGCTGGCG
CGCGGCGCGGAGGCGCTGGCGGCGCGCTTTGGCGCAGAAAAAAAGCTGTCCGGTGCGTCCGGCGTCTATTAA

Upstream 100 bases:

>100_bases
TCGTTCACCCGCCCGGTGCCGGCGCGCAAGGGCGACACTTTCCATGTCGATTACGGCAACATGGGCGCGATCAGTTGCCG
GTTTGTGTAAGGAAAAAACG

Downstream 100 bases:

>100_bases
GCCTGGCCCGGTAAGCGCAGCGCTACCGGGCAACCGTAGTACCCTACAAAAATTCCCATCAGAGGAAAAAAAATGAGCGA
CACATCATCTGCACTTCCGG

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKNAFKDALKVGRPQIGLWLGLANSYSAELLAGSGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGSALARASRWNRIPDYLHQANDAMCVLVQIETREAMSNLASI
LDVDGIDGVFIGPADLSADMGFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
RGAEALAARFGAEKKLSGASGVY

Sequences:

>Translated_263_residues
MKNAFKDALKVGRPQIGLWLGLANSYSAELLAGSGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGSALARASRWNRIPDYLHQANDAMCVLVQIETREAMSNLASI
LDVDGIDGVFIGPADLSADMGFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
RGAEALAARFGAEKKLSGASGVY
>Mature_263_residues
MKNAFKDALKVGRPQIGLWLGLANSYSAELLAGSGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIK
QLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGSALARASRWNRIPDYLHQANDAMCVLVQIETREAMSNLASI
LDVDGIDGVFIGPADLSADMGFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
RGAEALAARFGAEKKLSGASGVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=263, Percent_Identity=55.893536121673, Blast_Score=295, Evalue=2e-81,
Organism=Escherichia coli, GI1789514, Length=248, Percent_Identity=47.9838709677419, Blast_Score=231, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 27831; Mature: 27831

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAFKDALKVGRPQIGLWLGLANSYSAELLAGSGFDWLLIDGEHAPNNVQTVLTQLQAI
CCHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHEEHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPDYLHQANDAMCVLVQIETREAMSNLASILDVDGIDGVFIGPADLSADM
HHHHHHHCCCCHHHHHCCCCEEEEEEEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
GFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RGAEALAARFGAEKKLSGASGVY
HHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKNAFKDALKVGRPQIGLWLGLANSYSAELLAGSGFDWLLIDGEHAPNNVQTVLTQLQAI
CCHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHH
APYPSQPVVRPSWNDPVQIKQLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGS
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHEEHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
ALARASRWNRIPDYLHQANDAMCVLVQIETREAMSNLASILDVDGIDGVFIGPADLSADM
HHHHHHHCCCCHHHHHCCCCEEEEEEEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
GFAGNPQHPEVQAAIENAIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVDTTLLA
CCCCCCCCCHHHHHHHHHHEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHH
RGAEALAARFGAEKKLSGASGVY
HHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA