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The map label for this gene is narY [H]

Identifier: 194736461

GI number: 194736461

Start: 1635171

End: 1636715

Strand: Direct

Name: narY [H]

Synonym: SeSA_A1695

Alternate gene names: 194736461

Gene position: 1635171-1636715 (Clockwise)

Preceding gene: 194735877

Following gene: 194737060

Centisome position: 34.72

GC content: 53.59

Gene sequence:

>1545_bases
ATGAAAATACGCTCACAGGTTGGGATGGTACTGAATCTCGATAAATGTATTGGCTGTCACACCTGCTCCGTCACCTGTAA
AAACGTCTGGACCGGACGTGAAGGGATGGAATACGCATGGTTCAATAACGTCGAAACTAAACCGGGTATCGGCTATCCCA
AAAACTGGGAAGACCAGCAGGAGTGGCAAGGCGGTTGGGTTCGTGATGTTAATGGCAAGATAAGGCCCCGTCTGGGCGGC
AAAATGGGGGTAATTAGCAAAATTTTCGCCAACCCGGTGATTCCGCAAATCGATGATTACTATGAACCCTTTACCTTCGA
TTATCAGCATTTACATAATGCGCCGGAGAGTAAGCATCAACCCACCGCCCGTCCTCGTTCACTGATTGACGGAAAACGGA
TGGACAAGGTGATCTGGGGGCCAAACTGGGAAGAGCTGCTCGGCGGCGAGTTTGAAAAACGCGCCCGCGACCGTAACTTC
GACAACATACAAAAGGAGATGTACGGTCAGTTCGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCT
CAACCCCAGTTGTGTCTCCACTTGCCCAAGTGGCGCTATTTATAAGCGTGAAGAAGACGGCATCGTGCTAATCGACCAGG
ATAAATGCCGGGGCTGGCGTTTGTGTATCAGCGGCTGCCCGTACAAAAAAATCTACTTTAACTGGAAGAGCGGCAAGTCT
GAGAAGTGCATATTTTGCTATCCGCGTATTGAATCCGGTCAGCCGACCGTTTGTTCGGAAACCTGCGTTGGGCGCATCCG
TTATCTTGGCGTTCTGCTCTACGACGCCGACAGGATTGAAGAAGCGGCCAGTACCGAACACGAGACAGATTTGTATGAAC
GTCAGTGCGATGTGTTTCTCGATCCGCACGATCCGGCGGTCATCGAAGAGGCGCTTAAACAGGGCATCCCGCAAAACGTC
ATCGACGCCGCCCAACGGTCGCCGGTGTACAAAATGGCGATGGACTGGAAGCTGGCGCTTCCGCTGCACCCGGAATACCG
TACCCTGCCGATGGTCTGGTACGTACCGCCGCTGTCGCCCATCCAGTCATACGCTGACGCGGGCGGGTTGCCACACAATG
GCAATATTCTGCCGGCGGTGGAGACGCTGCGTATTCCGGTACAATACCTGGCCAATATGCTCAGCGCGGGCGATACCGGT
CCGGTCATTCGGGCGCTAAAGCGGATGATGGCGATGCGCCACTACATGCGCTCGCAGACGGTTGAAGGCGTGACGGATAC
CCGCGCCATTGAGGAAGTCGGTCTGAGCGTACAACAGGTTGAAGAGATGTATCGCTACCTGGCGATTGCCAATTATGAAG
ATCGTTTTGTCATTCCCACCAGCCACCGGGAAATGGCGCGCGACGCCTTTCCGGAGCGCAACGGCTGCGGCTTTACTTTT
GGCGACGGTTGTCACGGCTCGGATACCAAATTCAATCTGTTCAACAGTAGCCGTATTGATGCCATCAATATCACTGAAGT
ACGTGATAAAGCGGAGGGGGAATAA

Upstream 100 bases:

>100_bases
CCGTGGGTTCGAATCGCGATGAATTCATCATGATCAGGAAGATGAAAAACATTAACTGGCTGGATGATGAGGGCCGGGAT
CAGGTACAGGAGGCGAAAAA

Downstream 100 bases:

>100_bases
TGCAAATCCTCAAAGCGATTGGCCTGTTGATGGAGTATCCGGACGACGAGTTGTGGGAATATCGGGATGAAGCGCTTGCC
CTTATCCAACACGATGCGCC

Product: nitrate reductase 2, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQEWQGGWVRDVNGKIRPRLGG
KMGVISKIFANPVIPQIDDYYEPFTFDYQHLHNAPESKHQPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
DNIQKEMYGQFENTFMMYLPRLCEHCLNPSCVSTCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFLDPHDPAVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTG
PVIRALKRMMAMRHYMRSQTVEGVTDTRAIEEVGLSVQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE

Sequences:

>Translated_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQEWQGGWVRDVNGKIRPRLGG
KMGVISKIFANPVIPQIDDYYEPFTFDYQHLHNAPESKHQPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
DNIQKEMYGQFENTFMMYLPRLCEHCLNPSCVSTCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFLDPHDPAVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTG
PVIRALKRMMAMRHYMRSQTVEGVTDTRAIEEVGLSVQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE
>Mature_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQEWQGGWVRDVNGKIRPRLGG
KMGVISKIFANPVIPQIDDYYEPFTFDYQHLHNAPESKHQPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
DNIQKEMYGQFENTFMMYLPRLCEHCLNPSCVSTCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFLDPHDPAVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTG
PVIRALKRMMAMRHYMRSQTVEGVTDTRAIEEVGLSVQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=514, Percent_Identity=94.3579766536965, Blast_Score=1030, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=80.2371541501976, Blast_Score=877, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=99, Percent_Identity=42.4242424242424, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787122, Length=99, Percent_Identity=42.4242424242424, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=30.6306306306306, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=38.3838383838384, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=36.046511627907, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58797; Mature: 58797

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQ
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGGKMGVISKIFANPVIPQIDDYYEPFTFDYQHLHNAPESKHQ
HHCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFDNIQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVSTCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCHHHHHCCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHCCCEEE
DPHDPAVIEEALKQGIPQNVIDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTGPVIRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIEEVGLSVQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCCC
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCEEEEECCCCCCEEEHHHHHHHCCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQ
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGGKMGVISKIFANPVIPQIDDYYEPFTFDYQHLHNAPESKHQ
HHCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFDNIQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVSTCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCHHHHHCCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHCCCEEE
DPHDPAVIEEALKQGIPQNVIDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTGPVIRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIEEVGLSVQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCCC
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCEEEEECCCCCCEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]