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The map label for this gene is ynfL [H]

Identifier: 194736254

GI number: 194736254

Start: 1531536

End: 1532435

Strand: Direct

Name: ynfL [H]

Synonym: SeSA_A1589

Alternate gene names: 194736254

Gene position: 1531536-1532435 (Clockwise)

Preceding gene: 194736947

Following gene: 194736803

Centisome position: 32.52

GC content: 57.0

Gene sequence:

>900_bases
ATGAATATCGAATTACGTCATTTACGTTACTTTGTGGCGGTAGCAGAAGAGCTGCATTTTGGTCGCGCCGCCGCGCGCCT
CAATATCTCGCAGCCGCCGCTGAGTCAGCAGATACAAATTCTGGAGCAGCAGGTCGGCGCGCGATTACTGGCGCGGACCA
ACCGCAGCGTAGCGTTAACCGCCGCCGGGCGACAGTTTCTGGTGGATAGCCGACACATCCTGAGTCTGGTAAACGACGCC
GCCGCTCGCGCCGAGCGGTTGCATCAGGGCGAAGCGGGAGAAATCCGTATTGGCTTTACGTCTTCAGCGCCTTTTATTCG
GGCGGTATCGCATACCTTTTCGCTGTTCCGCCAGTCCTATCCGGGGGTGCATATGCAAACGCGTGAAATGAATACGCGTG
AGCAAATCGCGCCGCTCAACGAAGGCGTACTGGATATTGGCTTGCTGCGGAATACGCCGCTGCCCGACACACTTAATCGC
GAGGTGATTTTGCATGAGCCGCTTATGGCCATGATCCCGCGCGAACATCGGCTGGCGCAAAAGCCGGTCGTCAGTCTGAC
AGAACTGGCGGAAGAACCTTTCGTCTTTTTCGATCCGCATGTCGGGACGGGCCTCTACGACGATATTCTCGGCCTGATGC
GGCGCTATAACCTGTCGCCGACCATCACGCAGGAGGTTGGCGAGGCAATGACCATTATCGGTCTGGTGGCGGCGGGACTG
GGTGTCTCGATTCTGCCGGCATCCTTTAAGCGTGTACAGATGAGCGAGATGCGCTGGGTTCCCCTGGCAGAAGAGGACGC
CGTATCTGAAATGTGGCTGGTATGGCCAAAGCATCATGAACAGAGTCATGCCGTACAGCGGTTTTGCCAACTGCTATTAC
TGGCTGCCCGACGGGATTAA

Upstream 100 bases:

>100_bases
TCTCCGTCAACAGGAGACGGGTATAACTGACATGTAAAGCATAGAAAAAGCAAAATATTCTGTCTAATATATTAATTGTC
TCAAATAAGACGTTAAAAAT

Downstream 100 bases:

>100_bases
TTACGCTTTAAAAGGGTGAAAATGTGCAGTTAATCACACGGCTAAGCAAAAAGTTGACGGCGAGTATTGAAGTGTTTCAC
CATAGCACACAGATTATTTC

Product: LysR substrate binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQVGARLLARTNRSVALTAAGRQFLVDSRHILSLVNDA
AARAERLHQGEAGEIRIGFTSSAPFIRAVSHTFSLFRQSYPGVHMQTREMNTREQIAPLNEGVLDIGLLRNTPLPDTLNR
EVILHEPLMAMIPREHRLAQKPVVSLTELAEEPFVFFDPHVGTGLYDDILGLMRRYNLSPTITQEVGEAMTIIGLVAAGL
GVSILPASFKRVQMSEMRWVPLAEEDAVSEMWLVWPKHHEQSHAVQRFCQLLLLAARRD

Sequences:

>Translated_299_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQVGARLLARTNRSVALTAAGRQFLVDSRHILSLVNDA
AARAERLHQGEAGEIRIGFTSSAPFIRAVSHTFSLFRQSYPGVHMQTREMNTREQIAPLNEGVLDIGLLRNTPLPDTLNR
EVILHEPLMAMIPREHRLAQKPVVSLTELAEEPFVFFDPHVGTGLYDDILGLMRRYNLSPTITQEVGEAMTIIGLVAAGL
GVSILPASFKRVQMSEMRWVPLAEEDAVSEMWLVWPKHHEQSHAVQRFCQLLLLAARRD
>Mature_299_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQVGARLLARTNRSVALTAAGRQFLVDSRHILSLVNDA
AARAERLHQGEAGEIRIGFTSSAPFIRAVSHTFSLFRQSYPGVHMQTREMNTREQIAPLNEGVLDIGLLRNTPLPDTLNR
EVILHEPLMAMIPREHRLAQKPVVSLTELAEEPFVFFDPHVGTGLYDDILGLMRRYNLSPTITQEVGEAMTIIGLVAAGL
GVSILPASFKRVQMSEMRWVPLAEEDAVSEMWLVWPKHHEQSHAVQRFCQLLLLAARRD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=83.1649831649832, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI2367136, Length=257, Percent_Identity=38.5214007782101, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=33.448275862069, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=30.6451612903226, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788748, Length=252, Percent_Identity=30.5555555555556, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=26.1904761904762, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=34.3589743589744, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI157672245, Length=298, Percent_Identity=29.5302013422819, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788297, Length=256, Percent_Identity=27.734375, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790208, Length=258, Percent_Identity=27.906976744186, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789506, Length=295, Percent_Identity=23.0508474576271, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786713, Length=246, Percent_Identity=28.0487804878049, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787589, Length=129, Percent_Identity=37.2093023255814, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787530, Length=269, Percent_Identity=24.1635687732342, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=258, Percent_Identity=24.4186046511628, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=254, Percent_Identity=28.3464566929134, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787806, Length=299, Percent_Identity=23.7458193979933, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=25.5972696245734, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789173, Length=130, Percent_Identity=30.7692307692308, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33667; Mature: 33667

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQVGARLLARTNRSVALT
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGRQFLVDSRHILSLVNDAAARAERLHQGEAGEIRIGFTSSAPFIRAVSHTFSLFRQSY
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
PGVHMQTREMNTREQIAPLNEGVLDIGLLRNTPLPDTLNREVILHEPLMAMIPREHRLAQ
CCCEEECCCCCCHHHHCCCCCCCEEEHHHCCCCCCCCCCCCEEEECCHHHHCCCHHHHHH
KPVVSLTELAEEPFVFFDPHVGTGLYDDILGLMRRYNLSPTITQEVGEAMTIIGLVAAGL
CHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
GVSILPASFKRVQMSEMRWVPLAEEDAVSEMWLVWPKHHEQSHAVQRFCQLLLLAARRD
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQVGARLLARTNRSVALT
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGRQFLVDSRHILSLVNDAAARAERLHQGEAGEIRIGFTSSAPFIRAVSHTFSLFRQSY
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
PGVHMQTREMNTREQIAPLNEGVLDIGLLRNTPLPDTLNREVILHEPLMAMIPREHRLAQ
CCCEEECCCCCCHHHHCCCCCCCEEEHHHCCCCCCCCCCCCEEEECCHHHHCCCHHHHHH
KPVVSLTELAEEPFVFFDPHVGTGLYDDILGLMRRYNLSPTITQEVGEAMTIIGLVAAGL
CHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
GVSILPASFKRVQMSEMRWVPLAEEDAVSEMWLVWPKHHEQSHAVQRFCQLLLLAARRD
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]