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The map label for this gene is bioD2 [H]

Identifier: 194735939

GI number: 194735939

Start: 1533909

End: 1534604

Strand: Direct

Name: bioD2 [H]

Synonym: SeSA_A1591

Alternate gene names: 194735939

Gene position: 1533909-1534604 (Clockwise)

Preceding gene: 194736803

Following gene: 194734916

Centisome position: 32.57

GC content: 52.3

Gene sequence:

>696_bases
ATGCTGAAGCGTTTCTTTATTACAGGTACAGACACTTCTGTTGGGAAGACGGTGGTTTCCCGCGCATTACTACAAGCGTT
AGCGTCGGGTGGTAAGAGCGTTGCAGGGTATAAACCTGTCGCTAAAGGGAGCAAAGAGACTGCAGAAGGGATGCGCAACA
AAGACGCGCTGGTATTGCAAAGCGTCTCTTCGCTGGAGTTGCCGTATGAGGCGATTAATCCGATCGCGTTAAGCGAAGAA
GAAAGCAGCGTCGCGCATAGCTGTCCTATAAATTACACCTTGCTGTCCAACGGTCTGGCGAGCCTGAGCGATAAAGTGGA
CCACGTGGTTGTGGAGGGTACTGGCGGCTGGCGTAGTTTGATGAATGATTTACGTCCATTGTCCGAATGGGTGGTACAGG
AACAGTTACCGGTATTGATGGTGGTAGGGATTCAGGAGGGGTGCATTAATCATGCGCTACTGACTGCGCAAGCTGTCGCT
AACGATGGACTGCCATTAATTGGCTGGGTGGCGAATCGTATCAATCCTGGCCTGGCGCATTATGCTGAAATTATTGATGT
GCTTGGGAAAAAACTGCCAGCGCCGCTGATTGGCGAGTTGCCGTACCTGCCGCGCGCCGAGCAGCGTGAGCTGGGGCAGT
ATATTCGTTTGTCAATGCTCGGCAGCGTGCTGGCGGTAGATAGAATCATGGCGTAA

Upstream 100 bases:

>100_bases
TTGCGCTAACTCAAGCTGATTGCCCTTGCCATATCTTAGACTTTCTCCACTGTATTATTTTCCTGGCTTATATTTTCGAA
GCATAACGGTGGAGTTAGTG

Downstream 100 bases:

>100_bases
CGTTCGCGAGAGCACTGACGCGACTACACAGGCAATCAGCAGACCGGGGAGTAACTGATACTCGCCGGTCATTTCACAAA
TCATCAGGGTGGACATTATC

Product: putative dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASGGKSVAGYKPVAKGSKETAEGMRNKDALVLQSVSSLELPYEAINPIALSEE
ESSVAHSCPINYTLLSNGLASLSDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAVA
NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLSMLGSVLAVDRIMA

Sequences:

>Translated_231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASGGKSVAGYKPVAKGSKETAEGMRNKDALVLQSVSSLELPYEAINPIALSEE
ESSVAHSCPINYTLLSNGLASLSDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAVA
NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLSMLGSVLAVDRIMA
>Mature_231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASGGKSVAGYKPVAKGSKETAEGMRNKDALVLQSVSSLELPYEAINPIALSEE
ESSVAHSCPINYTLLSNGLASLSDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAVA
NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLSMLGSVLAVDRIMA

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081944, Length=229, Percent_Identity=93.4497816593886, Blast_Score=443, Evalue=1e-126,
Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=49.7777777777778, Blast_Score=221, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 24752; Mature: 24752

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRFFITGTDTSVGKTVVSRALLQALASGGKSVAGYKPVAKGSKETAEGMRNKDALVLQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
SVSSLELPYEAINPIALSEEESSVAHSCPINYTLLSNGLASLSDKVDHVVVEGTGGWRSL
HHHHHCCCHHHCCCCEECCCHHHHHHCCCCCHHHHHCCHHHHHHCCCEEEEECCCCHHHH
MNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAVANDGLPLIGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
YAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLSMLGSVLAVDRIMA
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKRFFITGTDTSVGKTVVSRALLQALASGGKSVAGYKPVAKGSKETAEGMRNKDALVLQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
SVSSLELPYEAINPIALSEEESSVAHSCPINYTLLSNGLASLSDKVDHVVVEGTGGWRSL
HHHHHCCCHHHCCCCEECCCHHHHHHCCCCCHHHHHCCHHHHHHCCCEEEEECCCCHHHH
MNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAVANDGLPLIGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
YAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLSMLGSVLAVDRIMA
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]