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The map label for this gene is truD [H]

Identifier: 194735561

GI number: 194735561

Start: 2974558

End: 2975607

Strand: Reverse

Name: truD [H]

Synonym: SeSA_A3079

Alternate gene names: 194735561

Gene position: 2975607-2974558 (Counterclockwise)

Preceding gene: 194738272

Following gene: 194734748

Centisome position: 63.19

GC content: 54.0

Gene sequence:

>1050_bases
ATGACAGAGTTTGACAATCTCACCTGGCTGCACGGAAAACCACAAGGTAGCGGATTGCTGAAAGCGAATCCGGAAGATTT
TGTGGTGGTTGAAGATTTAGGCTTTACGCCGGATGGTGAAGGCGAGCACATTCTGCTGCGTATTTTGAAAAACGGTTGCA
ATACCCGTTTTGTCGCTGACGCGCTGGCGAAATTCCTGAAAATTCACGCTCGCGAAGTGAGCTTTGCCGGGCAAAAAGAT
AAACACGCCGTTACCGAGCAGTGGCTGTGCGCGCGCGTGCCGGGGAAAGAGATGCCCGATTTCAGCGCCTTTCAGCTGGA
AGGTTGTAAAGTGCTGGAATACGCGCGTCACAAGCGTAAGCTGCGTTTAGGCGCGCTGAAAGGCAATGCCTTTACGCTGG
TGTTGCGTGAAATAAGCGATCGCCGCGATGTCGAAACGCGATTGCAGGCGATTCGCGATGGCGGCGTGCCGAACTATTTT
GGCGCACAGCGATTCGGCATTGGTGGCAGCAACTTACAGGGCGCGCTGCGCTGGGCGCAAAGTAACGCGCCGGTGCGCGA
TCGCAATAAACGCAGTTTTTGGTTGTCGGCGGCACGTAGCGCGTTGTTTAATCAAATTGTTCACCAGCGGCTGAAGAAAC
CAGACTTTAATCAAGTTGTTGACGGCGATGCGCTACAATTAGCGGGGCGTGGAAGCTGGTTCGTCGCGACATCTGAAGAA
CTGCCTGAATTGCAGCGCCGGGTAGACGAAAAAGAGCTGATGATTACAGCGTCGTTGCCGGGCAGCGGAGAGTGGGGAAC
ACAGCGTGCGGCGCTGGCGTTTGAGCAAGATGCCATTGCCCAGGAAACGGTGTTGCAATCGCTGTTGCTGCGCGAAAAAG
TAGAGGCGTCGCGCAGAGCGATGCTGCTTTATCCGCAACAATTAAGCTGGAACTGGTGGGATGACGTTACCGTCGAGTTA
CGTTTCTGGCTGCCCGCAGGCAGTTTTGCCACCAGCGTAGTAAGGGAACTGATCAACACAATGGGTGATTATGCGCATAT
TGCTGAGTAA

Upstream 100 bases:

>100_bases
ATGTTAACGTGAAGGCGACGACGACGGAAAAGCTCGGATTTACCGGACGCGGAGAAGGCATTGCCTGCGAAGCGGTCGCG
TTGCTGATGAAGGCGGCGAA

Downstream 100 bases:

>100_bases
CGATGACGGGGTTCACGCGCCCGGTATACAAACGCTGGCGAAAGCGCTGCGTGAGTTTGCTGATGTACAGGTCGTAGCCC
CGGATCGTAACCGCAGTGGC

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTEFDNLTWLHGKPQGSGLLKANPEDFVVVEDLGFTPDGEGEHILLRILKNGCNTRFVADALAKFLKIHAREVSFAGQKD
KHAVTEQWLCARVPGKEMPDFSAFQLEGCKVLEYARHKRKLRLGALKGNAFTLVLREISDRRDVETRLQAIRDGGVPNYF
GAQRFGIGGSNLQGALRWAQSNAPVRDRNKRSFWLSAARSALFNQIVHQRLKKPDFNQVVDGDALQLAGRGSWFVATSEE
LPELQRRVDEKELMITASLPGSGEWGTQRAALAFEQDAIAQETVLQSLLLREKVEASRRAMLLYPQQLSWNWWDDVTVEL
RFWLPAGSFATSVVRELINTMGDYAHIAE

Sequences:

>Translated_349_residues
MTEFDNLTWLHGKPQGSGLLKANPEDFVVVEDLGFTPDGEGEHILLRILKNGCNTRFVADALAKFLKIHAREVSFAGQKD
KHAVTEQWLCARVPGKEMPDFSAFQLEGCKVLEYARHKRKLRLGALKGNAFTLVLREISDRRDVETRLQAIRDGGVPNYF
GAQRFGIGGSNLQGALRWAQSNAPVRDRNKRSFWLSAARSALFNQIVHQRLKKPDFNQVVDGDALQLAGRGSWFVATSEE
LPELQRRVDEKELMITASLPGSGEWGTQRAALAFEQDAIAQETVLQSLLLREKVEASRRAMLLYPQQLSWNWWDDVTVEL
RFWLPAGSFATSVVRELINTMGDYAHIAE
>Mature_348_residues
TEFDNLTWLHGKPQGSGLLKANPEDFVVVEDLGFTPDGEGEHILLRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDK
HAVTEQWLCARVPGKEMPDFSAFQLEGCKVLEYARHKRKLRLGALKGNAFTLVLREISDRRDVETRLQAIRDGGVPNYFG
AQRFGIGGSNLQGALRWAQSNAPVRDRNKRSFWLSAARSALFNQIVHQRLKKPDFNQVVDGDALQLAGRGSWFVATSEEL
PELQRRVDEKELMITASLPGSGEWGTQRAALAFEQDAIAQETVLQSLLLREKVEASRRAMLLYPQQLSWNWWDDVTVELR
FWLPAGSFATSVVRELINTMGDYAHIAE

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI50727002, Length=132, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789102, Length=349, Percent_Identity=89.1117478510029, Blast_Score=642, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324817, Length=144, Percent_Identity=31.25, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 39333; Mature: 39202

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFDNLTWLHGKPQGSGLLKANPEDFVVVEDLGFTPDGEGEHILLRILKNGCNTRFVAD
CCCCCCEEEECCCCCCCCCEECCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHH
ALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDFSAFQLEGCKVLEYARHKRK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHH
LRLGALKGNAFTLVLREISDRRDVETRLQAIRDGGVPNYFGAQRFGIGGSNLQGALRWAQ
EEEEEECCCEEEEEEHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHC
SNAPVRDRNKRSFWLSAARSALFNQIVHQRLKKPDFNQVVDGDALQLAGRGSWFVATSEE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEECCCCEEEECCHH
LPELQRRVDEKELMITASLPGSGEWGTQRAALAFEQDAIAQETVLQSLLLREKVEASRRA
HHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
MLLYPQQLSWNWWDDVTVELRFWLPAGSFATSVVRELINTMGDYAHIAE
EEEECHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
TEFDNLTWLHGKPQGSGLLKANPEDFVVVEDLGFTPDGEGEHILLRILKNGCNTRFVAD
CCCCCEEEECCCCCCCCCEECCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHH
ALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDFSAFQLEGCKVLEYARHKRK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHH
LRLGALKGNAFTLVLREISDRRDVETRLQAIRDGGVPNYFGAQRFGIGGSNLQGALRWAQ
EEEEEECCCEEEEEEHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHC
SNAPVRDRNKRSFWLSAARSALFNQIVHQRLKKPDFNQVVDGDALQLAGRGSWFVATSEE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEECCCCEEEECCHH
LPELQRRVDEKELMITASLPGSGEWGTQRAALAFEQDAIAQETVLQSLLLREKVEASRRA
HHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
MLLYPQQLSWNWWDDVTVELRFWLPAGSFATSVVRELINTMGDYAHIAE
EEEECHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA