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The map label for this gene is tyrS [H]

Identifier: 194735512

GI number: 194735512

Start: 1487403

End: 1488677

Strand: Direct

Name: tyrS [H]

Synonym: SeSA_A1546

Alternate gene names: 194735512

Gene position: 1487403-1488677 (Clockwise)

Preceding gene: 194735612

Following gene: 194734833

Centisome position: 31.59

GC content: 52.78

Gene sequence:

>1275_bases
ATGGCAAGCAGTAACTTGATTAAACAATTGCAAGAGCGGGGGCTGGTAGCCCAGGTGACGGACGAAGACGCGTTGGCAGA
GCGACTGGCGCAAGGCCCGATCGCGCTCTATTGCGGCTTCGATCCTACCGCTGACAGCTTGCATTTGGGGCATCTGGTTC
CATTGTTATGCCTGAAACGCTTCCAGCAGGCAGGGCACAAACCTGTTGCGCTGGTAGGCGGCGCGACCGGTCTGATTGGC
GACCCGAGCTTCAAAGCCGCCGAGCGTAAACTGAACACTGAAGAGACCGTTCAGGAGTGGGTGGCGAAAATTCGTAAACA
GGTTGCCCCATTCCTGGACTTTGATTGCGGCGAAAACTCCGCCATCGCGGCCAATAACTATGACTGGTTCGGCAGCATGA
ATGTGCTGACTTTCCTGCGCGATATCGGTAAACATTTTTCCGTTAATCAGATGATCAACAAAGAAGCGGTGAAGCAGCGT
CTGAACCGTGACGATCAGGGTATCTCTTTCACGGAGTTCTCTTATAACCTGCTACAGGGGTACGACTTCGCTTGTCTGAA
TAAACTGCACGGCGTTGCCTTACAGATTGGCGGTTCTGACCAGTGGGGGAACATCACTTCCGGTATCGACCTGACCCGTC
GCCTGCACCAGAATCAGGTATTTGGTTTGACCGTTCCACTGATCACCAAAGCTGACGGCACGAAATTCGGTAAAACCGAA
GGCGGCGCTGTCTGGCTGGATCCGAAGAAAACCAGTCCGTACAAATTCTACCAGTTCTGGATTAACACCGCAGATGCCGA
TGTTTATCGCTTCCTGAAATTCTTCACTTTTATGGACATTGAAGAGATCAATGCCCTGGAAGAAGAAGATAAAAACAGCG
GGAAAGCGCCGCGCGCGCAGTATGTGCTGGCCGAGCAGGTGACGCGCCTGGTTCATGGTGAAGAAGGGCTGGTCGCGGCG
AAACGGATCACTGAATGTCTGTTCAGCGGTTCACTGAGCGCGCTGAGCGAAGCTGACTTTGAGCAGTTGGCGCAGGACGG
CGTACCGATGGTTGAAATGGAAAAAGGCGCCGATCTGATGCAGGCGCTGGTAGACGCCGAGCTCCAGCCGTCACGTGGTC
AGGCGCGTAAGACTATCGCCTCCAACGCTGTCACTATCAATGGTGAAAAACAATCCGATCCTGAATACATCTTTAACGAT
GAGGACCGTCTGTTTGGCCGCTATACCTTATTACGTCGCGGCAAAAAGAACTATTGTCTGATTTGCTGGAAATAA

Upstream 100 bases:

>100_bases
TGGTGCGGATGAATCCGCCGTTTTATGCTATGTCTCTTTCGCGTCTGGCGAAAAGTCGTGTACCGGCAAAGGTGCAGTCG
TTTTATACATGGAGATTTTG

Downstream 100 bases:

>100_bases
GGCATTAAGTAACAGGGAGTGAGAAATCACTCCCTTATTTTTGATGTTCAGGTAAAAAAATGAAGAATATCCTCGCTATC
CAGTCCCATGTTGTTTTCGG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MASSNLIKQLQERGLVAQVTDEDALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIG
DPSFKAAERKLNTEETVQEWVAKIRKQVAPFLDFDCGENSAIAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNRDDQGISFTEFSYNLLQGYDFACLNKLHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLVAA
KRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLMQALVDAELQPSRGQARKTIASNAVTINGEKQSDPEYIFND
EDRLFGRYTLLRRGKKNYCLICWK

Sequences:

>Translated_424_residues
MASSNLIKQLQERGLVAQVTDEDALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIG
DPSFKAAERKLNTEETVQEWVAKIRKQVAPFLDFDCGENSAIAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNRDDQGISFTEFSYNLLQGYDFACLNKLHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLVAA
KRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLMQALVDAELQPSRGQARKTIASNAVTINGEKQSDPEYIFND
EDRLFGRYTLLRRGKKNYCLICWK
>Mature_423_residues
ASSNLIKQLQERGLVAQVTDEDALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGD
PSFKAAERKLNTEETVQEWVAKIRKQVAPFLDFDCGENSAIAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQRL
NRDDQGISFTEFSYNLLQGYDFACLNKLHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTEG
GAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLVAAK
RITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLMQALVDAELQPSRGQARKTIASNAVTINGEKQSDPEYIFNDE
DRLFGRYTLLRRGKKNYCLICWK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=407, Percent_Identity=37.5921375921376, Blast_Score=252, Evalue=4e-67,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=96.4622641509434, Blast_Score=854, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25145802, Length=411, Percent_Identity=35.7664233576642, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI25145805, Length=334, Percent_Identity=33.2335329341317, Blast_Score=168, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=36.3013698630137, Blast_Score=273, Evalue=3e-74,
Organism=Drosophila melanogaster, GI20130383, Length=447, Percent_Identity=37.1364653243848, Blast_Score=298, Evalue=4e-81,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47282; Mature: 47151

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSNLIKQLQERGLVAQVTDEDALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
CCCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVAKIRKQVAPFLDFDCGENS
HHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
AIAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDQGISFTEFSYNLLQG
EEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHC
YDFACLNKLHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
CCHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCC
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIEEINALEEEDKNSGKAPRAQ
CCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHH
YVLAEQVTRLVHGEEGLVAAKRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHH
QALVDAELQPSRGQARKTIASNAVTINGEKQSDPEYIFNDEDRLFGRYTLLRRGKKNYCL
HHHHCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEE
ICWK
EEEC
>Mature Secondary Structure 
ASSNLIKQLQERGLVAQVTDEDALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
CCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVAKIRKQVAPFLDFDCGENS
HHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
AIAANNYDWFGSMNVLTFLRDIGKHFSVNQMINKEAVKQRLNRDDQGISFTEFSYNLLQG
EEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHC
YDFACLNKLHGVALQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
CCHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCC
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMDIEEINALEEEDKNSGKAPRAQ
CCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHH
YVLAEQVTRLVHGEEGLVAAKRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHH
QALVDAELQPSRGQARKTIASNAVTINGEKQSDPEYIFNDEDRLFGRYTLLRRGKKNYCL
HHHHCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEE
ICWK
EEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA