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The map label for this gene is ygbJ [H]

Identifier: 194735274

GI number: 194735274

Start: 2966041

End: 2966964

Strand: Reverse

Name: ygbJ [H]

Synonym: SeSA_A3068

Alternate gene names: 194735274

Gene position: 2966964-2966041 (Counterclockwise)

Preceding gene: 194734084

Following gene: 194734714

Centisome position: 63.01

GC content: 59.74

Gene sequence:

>924_bases
ATGACAACAGGAACTGATTTTCATGTCGGCATTGTGGGGCTTGGCTCAATGGGAATGGGCGCTGCGCGTTCATGCCTGCG
CGCCGGGCTTTCCACATGGGGGGCCGATCTTAACCCGCAAGCGTGCGCGAATCTGCTCGCTGAAGGGGCTTGTGGGGCTG
CCGCCAGCGCCAGGGAGTTCGCCGGCGTCGTGGATGCGTTAGTCATTCTGGTCGTCAATGCCGCCCAGGTCAGACAGGTG
TTATTCGGTGAGGATGGCGTCGCCCATCTGATGAAACCCGGAAGCGCGGTGATGGTTTCGTCTACGATCTCAAGCGCCGA
CGCGCAAGAGATCGCCGCGGCGTTGACGGCATTGAATCTTAACATACTGGATGCGCCGGTTTCCGGCGGCGCGGTAAAAG
CGGCGCAGGGCGAAATGACGGTGATGGCCTCCGGGAACGAGGCGGCGTTTACCCGCCTGAAGCCTGTGCTGGACGCCGTT
GCCAGCAACGTTTATCGCATCAGCGATACGCCGGGCGCAGGCTCAACCGTCAAAATCATTCATCAACTGCTGGCTGGCGT
GCATATTGCGGCGGCGGCCGAAGCGATGGCGCTGGCCGCGCGGGCCGGTATTCCTCTGGACGTGATGTATGACGTCGTGA
CCCATGCCGCCGGCAACTCCTGGATGTTCGAAAACCGTATGCAGCATGTCGTTGACGGCGACTATACCCCGCATTCGGCC
GTCGACATTTTTGTCAAAGATTTGGGGCTGGTTGCCGACACGGCGAAAGCGCTGCGCTTCCCCTTACCGCTGGCCTCTAC
GGCGTTAAATATGTTCACCAGCGCCAGTAACGCCGGTTATGGCAAAGAGGATGACAGCGCTGTGATCAAAATTTTCTCGG
GGATTACTCTGCCTGGCGTCACGCCTGAGGAGCCGTCATGTTAA

Upstream 100 bases:

>100_bases
TTTTGTTATTTTTTGTGAAACAACTCACCGCAATTGTTTACAAAAACGCCTATATTCACATAACAAAACATAATATCACG
TAAATTAACATGGAGTGATA

Downstream 100 bases:

>100_bases
AAATTGGCGTCATTGCCGATGATTTTACCGGCGCGACTGACATCGCCAGTTTTCTGGTCGAAAACGGAATGCCGACAGTG
CAGATCAATGATGTCCCAAC

Product: 6-phosphogluconate dehydrogenase NAD-binding

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTTGTDFHVGIVGLGSMGMGAARSCLRAGLSTWGADLNPQACANLLAEGACGAAASAREFAGVVDALVILVVNAAQVRQV
LFGEDGVAHLMKPGSAVMVSSTISSADAQEIAAALTALNLNILDAPVSGGAVKAAQGEMTVMASGNEAAFTRLKPVLDAV
ASNVYRISDTPGAGSTVKIIHQLLAGVHIAAAAEAMALAARAGIPLDVMYDVVTHAAGNSWMFENRMQHVVDGDYTPHSA
VDIFVKDLGLVADTAKALRFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGVTPEEPSC

Sequences:

>Translated_307_residues
MTTGTDFHVGIVGLGSMGMGAARSCLRAGLSTWGADLNPQACANLLAEGACGAAASAREFAGVVDALVILVVNAAQVRQV
LFGEDGVAHLMKPGSAVMVSSTISSADAQEIAAALTALNLNILDAPVSGGAVKAAQGEMTVMASGNEAAFTRLKPVLDAV
ASNVYRISDTPGAGSTVKIIHQLLAGVHIAAAAEAMALAARAGIPLDVMYDVVTHAAGNSWMFENRMQHVVDGDYTPHSA
VDIFVKDLGLVADTAKALRFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGVTPEEPSC
>Mature_306_residues
TTGTDFHVGIVGLGSMGMGAARSCLRAGLSTWGADLNPQACANLLAEGACGAAASAREFAGVVDALVILVVNAAQVRQVL
FGEDGVAHLMKPGSAVMVSSTISSADAQEIAAALTALNLNILDAPVSGGAVKAAQGEMTVMASGNEAAFTRLKPVLDAVA
SNVYRISDTPGAGSTVKIIHQLLAGVHIAAAAEAMALAARAGIPLDVMYDVVTHAAGNSWMFENRMQHVVDGDYTPHSAV
DIFVKDLGLVADTAKALRFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGVTPEEPSC

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=28.5223367697594, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI40556376, Length=290, Percent_Identity=23.1034482758621, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1789092, Length=299, Percent_Identity=81.9397993311037, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI145693186, Length=282, Percent_Identity=33.3333333333333, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1786719, Length=284, Percent_Identity=29.9295774647887, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=30.1369863013699, Blast_Score=120, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17557316, Length=271, Percent_Identity=28.7822878228782, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24655230, Length=298, Percent_Identity=28.8590604026846, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922568, Length=298, Percent_Identity=28.8590604026846, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=25.0883392226148, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31252; Mature: 31121

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGTDFHVGIVGLGSMGMGAARSCLRAGLSTWGADLNPQACANLLAEGACGAAASAREF
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHH
AGVVDALVILVVNAAQVRQVLFGEDGVAHLMKPGSAVMVSSTISSADAQEIAAALTALNL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECHHCCCCHHHHHHHHHHHCC
NILDAPVSGGAVKAAQGEMTVMASGNEAAFTRLKPVLDAVASNVYRISDTPGAGSTVKII
EEEECCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHH
HQLLAGVHIAAAAEAMALAARAGIPLDVMYDVVTHAAGNSWMFENRMQHVVDGDYTPHSA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHH
VDIFVKDLGLVADTAKALRFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCC
TPEEPSC
CCCCCCC
>Mature Secondary Structure 
TTGTDFHVGIVGLGSMGMGAARSCLRAGLSTWGADLNPQACANLLAEGACGAAASAREF
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHH
AGVVDALVILVVNAAQVRQVLFGEDGVAHLMKPGSAVMVSSTISSADAQEIAAALTALNL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECHHCCCCHHHHHHHHHHHCC
NILDAPVSGGAVKAAQGEMTVMASGNEAAFTRLKPVLDAVASNVYRISDTPGAGSTVKII
EEEECCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHH
HQLLAGVHIAAAAEAMALAARAGIPLDVMYDVVTHAAGNSWMFENRMQHVVDGDYTPHSA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHH
VDIFVKDLGLVADTAKALRFPLPLASTALNMFTSASNAGYGKEDDSAVIKIFSGITLPGV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCC
TPEEPSC
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]