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The map label for this gene is recE [H]

Identifier: 194734908

GI number: 194734908

Start: 1944300

End: 1945406

Strand: Direct

Name: recE [H]

Synonym: SeSA_A2022

Alternate gene names: 194734908

Gene position: 1944300-1945406 (Clockwise)

Preceding gene: 194737529

Following gene: 194734720

Centisome position: 41.29

GC content: 51.22

Gene sequence:

>1107_bases
GTGCCTGATAACACCCCAACAACCCATGAAGAAAATGCAGCAAGGCTTCGTCAGGCGGGCAAATGTCTGCGGGATATTGA
GGCAGGGAGATTTCAGTGTGATGAAGAAAAACCGCAACCGACAGGCGAACTGGCAGATGAACCAGCAACGCCTGAAGCAG
TGGAACAGGACACAACTGAACATCATCCGGATCCACAGCCGCTGGAGAATGAGCCACCTGTAAGCCAGACAGAAGCAGGC
TACCAGAAAATACGGGCAGAACTGCACGAAGCACGTAAAAACATTCCACCCAAAAACCCGGTTGATGTTGGTAAACAACT
GGCAGCCGTGCGCGGTGAATATGCCGAAGACATCAGCGACCCGAACGATCCGAAGTGGGTTCCTAACAATTACAGCGCCT
CAAATCAGGGTGAAAAAGAAGAAGTGGTGCCGGAGGAAAAACAACTAGCAGCAGAGCCGGAGGCTGTCACCAGAAACGCG
GACGGGACTTTCGATGTATCAGCGCTATTTCCGCCCCCCTCAAACCAGACCGAAAAAACGGAAGCCAGAACAGAAAGAGA
TGGAGAAACGCCGAAAGAGAGCAACCAGCAGGAAACGGCTGGCGATACAGGGCAGGAAATTACAACGGACGGTGGATCAG
GTACTGGCGGTGATGAAGCTGGCGAAGCGGCAGATCCCGTAGAAAACGGAAATTTCACTGTCCCTGATGATATACAGCCA
GGCATTTACTATGACATCCCTAACGAGGCGTATCACGCTGGCCCGGGGGTCAGTAAATCACAGCTTGATGATATCGCAGA
TACACCAGCAATTTATCTTTGGCGCAAAAATGCCCCCGTGGACACGGAGAAAACAAAATCTCTCGATACAGGAACGGCTT
TTCACTGCCGGGTACTGGAACCAGAGGAATTCAGTAAACGCTTCATCATCGCACCGGAGTTTAACCGCCGTACCAGTGCA
GGAAAAGAAGAAGAGAAAACCTTTCTGGAAGAGTGCGCACGGACAGGAATAACCGTGCTTACGGCAGAAGAAGGCCGGAA
AATCGAACTTATGTACCAGAGTGTGATGGCGTTAACCGAGTGCATTGCTGGAGAAGTTGATCAGTGA

Upstream 100 bases:

>100_bases
TGGTGTAATTCTCCAGCCCAGTAATCCTGATCTTCGCCGGACGTGGTGCGAAAGCTTTGTTGCCGTTCATGGCGTTCGCG
ATCCGGACTGGTACGAATAT

Downstream 100 bases:

>100_bases
GTCGAAAAAAGTACGATGCTAACCTCCCGAGGAACCTCACCTACCGTAAGGCCAGTAAATCTTTTTTCTGGCGTAACCCG
GTAACTGACAAGGAATTTCC

Product: exodeoxyribonuclease 8

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MPDNTPTTHEENAARLRQAGKCLRDIEAGRFQCDEEKPQPTGELADEPATPEAVEQDTTEHHPDPQPLENEPPVSQTEAG
YQKIRAELHEARKNIPPKNPVDVGKQLAAVRGEYAEDISDPNDPKWVPNNYSASNQGEKEEVVPEEKQLAAEPEAVTRNA
DGTFDVSALFPPPSNQTEKTEARTERDGETPKESNQQETAGDTGQEITTDGGSGTGGDEAGEAADPVENGNFTVPDDIQP
GIYYDIPNEAYHAGPGVSKSQLDDIADTPAIYLWRKNAPVDTEKTKSLDTGTAFHCRVLEPEEFSKRFIIAPEFNRRTSA
GKEEEKTFLEECARTGITVLTAEEGRKIELMYQSVMALTECIAGEVDQ

Sequences:

>Translated_368_residues
MPDNTPTTHEENAARLRQAGKCLRDIEAGRFQCDEEKPQPTGELADEPATPEAVEQDTTEHHPDPQPLENEPPVSQTEAG
YQKIRAELHEARKNIPPKNPVDVGKQLAAVRGEYAEDISDPNDPKWVPNNYSASNQGEKEEVVPEEKQLAAEPEAVTRNA
DGTFDVSALFPPPSNQTEKTEARTERDGETPKESNQQETAGDTGQEITTDGGSGTGGDEAGEAADPVENGNFTVPDDIQP
GIYYDIPNEAYHAGPGVSKSQLDDIADTPAIYLWRKNAPVDTEKTKSLDTGTAFHCRVLEPEEFSKRFIIAPEFNRRTSA
GKEEEKTFLEECARTGITVLTAEEGRKIELMYQSVMALTECIAGEVDQ
>Mature_367_residues
PDNTPTTHEENAARLRQAGKCLRDIEAGRFQCDEEKPQPTGELADEPATPEAVEQDTTEHHPDPQPLENEPPVSQTEAGY
QKIRAELHEARKNIPPKNPVDVGKQLAAVRGEYAEDISDPNDPKWVPNNYSASNQGEKEEVVPEEKQLAAEPEAVTRNAD
GTFDVSALFPPPSNQTEKTEARTERDGETPKESNQQETAGDTGQEITTDGGSGTGGDEAGEAADPVENGNFTVPDDIQPG
IYYDIPNEAYHAGPGVSKSQLDDIADTPAIYLWRKNAPVDTEKTKSLDTGTAFHCRVLEPEEFSKRFIIAPEFNRRTSAG
KEEEKTFLEECARTGITVLTAEEGRKIELMYQSVMALTECIAGEVDQ

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=349, Percent_Identity=56.4469914040115, Blast_Score=337, Evalue=7e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 40333; Mature: 40202

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDNTPTTHEENAARLRQAGKCLRDIEAGRFQCDEEKPQPTGELADEPATPEAVEQDTTE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHH
HHPDPQPLENEPPVSQTEAGYQKIRAELHEARKNIPPKNPVDVGKQLAAVRGEYAEDISD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PNDPKWVPNNYSASNQGEKEEVVPEEKQLAAEPEAVTRNADGTFDVSALFPPPSNQTEKT
CCCCCCCCCCCCCCCCCCHHHCCCCHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCHHH
EARTERDGETPKESNQQETAGDTGQEITTDGGSGTGGDEAGEAADPVENGNFTVPDDIQP
HHHHHHCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GIYYDIPNEAYHAGPGVSKSQLDDIADTPAIYLWRKNAPVDTEKTKSLDTGTAFHCRVLE
CEEEECCCCHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHCCCCCCCEEEEEEEC
PEEFSKRFIIAPEFNRRTSAGKEEEKTFLEECARTGITVLTAEEGRKIELMYQSVMALTE
HHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHH
CIAGEVDQ
HHHCCCCC
>Mature Secondary Structure 
PDNTPTTHEENAARLRQAGKCLRDIEAGRFQCDEEKPQPTGELADEPATPEAVEQDTTE
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHH
HHPDPQPLENEPPVSQTEAGYQKIRAELHEARKNIPPKNPVDVGKQLAAVRGEYAEDISD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PNDPKWVPNNYSASNQGEKEEVVPEEKQLAAEPEAVTRNADGTFDVSALFPPPSNQTEKT
CCCCCCCCCCCCCCCCCCHHHCCCCHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCHHH
EARTERDGETPKESNQQETAGDTGQEITTDGGSGTGGDEAGEAADPVENGNFTVPDDIQP
HHHHHHCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GIYYDIPNEAYHAGPGVSKSQLDDIADTPAIYLWRKNAPVDTEKTKSLDTGTAFHCRVLE
CEEEECCCCHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHCCCCCCCEEEEEEEC
PEEFSKRFIIAPEFNRRTSAGKEEEKTFLEECARTGITVLTAEEGRKIELMYQSVMALTE
HHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHH
CIAGEVDQ
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]