Click here to switch to the map view.

The map label for this gene is intE [H]

Identifier: 194734720

GI number: 194734720

Start: 1945403

End: 1946482

Strand: Direct

Name: intE [H]

Synonym: SeSA_A2023

Alternate gene names: 194734720

Gene position: 1945403-1946482 (Clockwise)

Preceding gene: 194734908

Following gene: 194734509

Centisome position: 41.31

GC content: 47.96

Gene sequence:

>1080_bases
GTGAGTCGAAAAAAGTACGATGCTAACCTCCCGAGGAACCTCACCTACCGTAAGGCCAGTAAATCTTTTTTCTGGCGTAA
CCCGGTAACTGACAAGGAATTTCCGCTCGGTCAGATCGCCCGCAGGGACGCTATCACACAGACCATAGAGGCAAACAACT
TCATAGCGCAAAACCACACGCCAGTGGCGCTTATTGAAAAGCTAAAAGGAACGGACTCATTCACTGTGTCCGCATGGATT
GATCGCTATGAGGTTTTATTACAGCGCCGGAGTCTGTCGGTTAATACCTACAAGATTCGCGGTAATCAATTAGTGACCGT
ACGCGAAAAAATGGGGGAAATAATACTGGCAGAAGTAACAACCCGGCACATTGCCAAGTTTCTTGAGTCGTGGATAACCG
AGGGAAAAAACACTATGGCGGGAGCAATGAGATCAGTTCTATCTGACATGTTCAGAGAAGCTATTGTCGAAGGGCATATT
GTGAAAAACCCGGTGGAAGCAACCCGGATACCAGAGGTTAAGGTTGCCAGGGAACGCTTGCAACTGGAAACGTATAACGC
CACACGAGCGGCAGCAGAGCATATGCCTGCATGGTTCCCTCTCGCGATGGATTTAGCGCTCGTTACTGGTCAACGTAGGG
AGGATATCGTAAATATGAAATTTAGTGATGTTTTTGACAACCGCTTATACGTAACTCAGATTAAAACCGGAATGAAAATA
GCCATTCCCCTCTCCCTGACACTTCGGGCGACGGGGTTACGTCTGGGAACGGTAATCGATCGCTGCCGACTGGTAAGCCG
CACTGATTTCATGATCAGTGCCGGAATCAGGAAAAATAGCCCGACCGGGAATATTCACCCGGATGGGCTGACAAAGAAAT
TTGTAAAAGCCAGAAAAATTTCAGGCGTTAAATTTAGTGATAACCCACCGACATTTCACGAGATCCGTAGCCTGGCTGGT
CGGCTGTACAAAGACGAACGCGTCGAGGAATTCGCTCAAAAACTACTGGGCCACACCTCAGAGAACACCACGAAACTCTA
TCTCGATGAACGCGATAATAAAGCTTACGTGATGCTCTGA

Upstream 100 bases:

>100_bases
CAGGAATAACCGTGCTTACGGCAGAAGAAGGCCGGAAAATCGAACTTATGTACCAGAGTGTGATGGCGTTAACCGAGTGC
ATTGCTGGAGAAGTTGATCA

Downstream 100 bases:

>100_bases
TTTTGTTGTAAAAGAAATGTTAAACTGGATTTGGATGTGATATAAACAAAAAGACCGGAATACAGAAATTCGAGGAAATT
TCGAGGAATTTCGAGGGGAA

Product: phage integrase

Products: NA

Alternate protein names: Int(Lambda); Prophage e14 integrase [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSRKKYDANLPRNLTYRKASKSFFWRNPVTDKEFPLGQIARRDAITQTIEANNFIAQNHTPVALIEKLKGTDSFTVSAWI
DRYEVLLQRRSLSVNTYKIRGNQLVTVREKMGEIILAEVTTRHIAKFLESWITEGKNTMAGAMRSVLSDMFREAIVEGHI
VKNPVEATRIPEVKVARERLQLETYNATRAAAEHMPAWFPLAMDLALVTGQRREDIVNMKFSDVFDNRLYVTQIKTGMKI
AIPLSLTLRATGLRLGTVIDRCRLVSRTDFMISAGIRKNSPTGNIHPDGLTKKFVKARKISGVKFSDNPPTFHEIRSLAG
RLYKDERVEEFAQKLLGHTSENTTKLYLDERDNKAYVML

Sequences:

>Translated_359_residues
MSRKKYDANLPRNLTYRKASKSFFWRNPVTDKEFPLGQIARRDAITQTIEANNFIAQNHTPVALIEKLKGTDSFTVSAWI
DRYEVLLQRRSLSVNTYKIRGNQLVTVREKMGEIILAEVTTRHIAKFLESWITEGKNTMAGAMRSVLSDMFREAIVEGHI
VKNPVEATRIPEVKVARERLQLETYNATRAAAEHMPAWFPLAMDLALVTGQRREDIVNMKFSDVFDNRLYVTQIKTGMKI
AIPLSLTLRATGLRLGTVIDRCRLVSRTDFMISAGIRKNSPTGNIHPDGLTKKFVKARKISGVKFSDNPPTFHEIRSLAG
RLYKDERVEEFAQKLLGHTSENTTKLYLDERDNKAYVML
>Mature_358_residues
SRKKYDANLPRNLTYRKASKSFFWRNPVTDKEFPLGQIARRDAITQTIEANNFIAQNHTPVALIEKLKGTDSFTVSAWID
RYEVLLQRRSLSVNTYKIRGNQLVTVREKMGEIILAEVTTRHIAKFLESWITEGKNTMAGAMRSVLSDMFREAIVEGHIV
KNPVEATRIPEVKVARERLQLETYNATRAAAEHMPAWFPLAMDLALVTGQRREDIVNMKFSDVFDNRLYVTQIKTGMKIA
IPLSLTLRATGLRLGTVIDRCRLVSRTDFMISAGIRKNSPTGNIHPDGLTKKFVKARKISGVKFSDNPPTFHEIRSLAGR
LYKDERVEEFAQKLLGHTSENTTKLYLDERDNKAYVML

Specific function: Integrase from the cryptic lambdoic prophage e14. Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the h

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1787386, Length=358, Percent_Identity=38.268156424581, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016177
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR015094
- InterPro:   IPR023109
- InterPro:   IPR013087 [H]

Pfam domain/function: PF09003 Phage_integ_N; PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 41006; Mature: 40875

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKKYDANLPRNLTYRKASKSFFWRNPVTDKEFPLGQIARRDAITQTIEANNFIAQNHT
CCCCCCCCCCCCCCCHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCC
PVALIEKLKGTDSFTVSAWIDRYEVLLQRRSLSVNTYKIRGNQLVTVREKMGEIILAEVT
HHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHHHCCEEEHHHH
TRHIAKFLESWITEGKNTMAGAMRSVLSDMFREAIVEGHIVKNPVEATRIPEVKVARERL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHH
QLETYNATRAAAEHMPAWFPLAMDLALVTGQRREDIVNMKFSDVFDNRLYVTQIKTGMKI
HHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHCCCEEEEEECCCCEE
AIPLSLTLRATGLRLGTVIDRCRLVSRTDFMISAGIRKNSPTGNIHPDGLTKKFVKARKI
EEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
SGVKFSDNPPTFHEIRSLAGRLYKDERVEEFAQKLLGHTSENTTKLYLDERDNKAYVML
CCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEC
>Mature Secondary Structure 
SRKKYDANLPRNLTYRKASKSFFWRNPVTDKEFPLGQIARRDAITQTIEANNFIAQNHT
CCCCCCCCCCCCCCHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCC
PVALIEKLKGTDSFTVSAWIDRYEVLLQRRSLSVNTYKIRGNQLVTVREKMGEIILAEVT
HHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHHHCCEEEHHHH
TRHIAKFLESWITEGKNTMAGAMRSVLSDMFREAIVEGHIVKNPVEATRIPEVKVARERL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHH
QLETYNATRAAAEHMPAWFPLAMDLALVTGQRREDIVNMKFSDVFDNRLYVTQIKTGMKI
HHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHCCCEEEEEECCCCEE
AIPLSLTLRATGLRLGTVIDRCRLVSRTDFMISAGIRKNSPTGNIHPDGLTKKFVKARKI
EEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
SGVKFSDNPPTFHEIRSLAGRLYKDERVEEFAQKLLGHTSENTTKLYLDERDNKAYVML
CCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]