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The map label for this gene is ptsP [H]

Identifier: 194734275

GI number: 194734275

Start: 3064185

End: 3066431

Strand: Reverse

Name: ptsP [H]

Synonym: SeSA_A3167

Alternate gene names: 194734275

Gene position: 3066431-3064185 (Counterclockwise)

Preceding gene: 194734774

Following gene: 194736348

Centisome position: 65.12

GC content: 56.83

Gene sequence:

>2247_bases
ATGCTCACTCGCCTGCGCGAAATAGTCGAAAAAGTGGCCAGTGCGCCGCGTCTGAACGAGGCGCTGAATATTCTGGTCAC
GGATATCTGTCTTGCGATGGATACAGAGGTTTGCTCGGTTTACCTGGCCGATCATGACCGACGCTGTTATTACCTGATGG
CGACGCGGGGGTTGAAAAAACCGCGTGGTCGAACCGTTGCGCTTGCGTTTGATGAGGGGATCGTCGGTCTGGTGGGCAGG
CTGGCGGAACCCATTAACCTTGCCGATGCGCAAAAACATCCCAGCTTTAAATACATTCCTTCGGTAAAAGAGGAACGTTT
TCGCGCGTTTCTCGGCGTGCCGATCATTCAGCGTCGCCAACTTCTCGGCGTGCTGGTCGTACAGCAGCGCGAATTGCGCC
AGTACGATGAAAGCGAAGAGTCTTTCCTCGTCACGCTCGCTACGCAGATGGCGGCTATCCTGTCACAGTCGCAGGTCACA
GCGCTGTTCGGGCAATACCGGCAGACGCGAATCCGCGCGCTTCCTGCGGCGCCGGGCGTGGCTATCGCAACGGGCTGGCA
AGACGCGACCATGCCGCTGATGGAACAGGTTTACGAAGCGTCTACGCTGGATACCTCTCTTGAACGCGAACGACTGACCG
GCGCGCTGGAAGAGGCGGCGAACGAATTCCGACGATACAGCAAACGCTTTGCCGCCGGCGCGCAAAAAGAGACGGCGGCC
ATTTTCGATCTGTACTCACACCTGCTTTCAGATGCCAGACTGCGTCGCGAACTCTTCGCCGAGGTCGACAAAGGCGCTGT
TGCGGAGTGGGCCGTTAAAAAAATCATCGAAAAATTTGCCGAGCAATTTGCGGCGCTAACCGATAACTACCTCAAAGAAC
GTGCGGGAGACTTACGCACGCTGGGGCAGCGGCTGTTATTCCACCTTGATGATTCAGTTCAGGGGCCAAACGCTTGGCCG
GAACGTTTTATTCTGGTCGCGGATGAGCTGTCGGCGACCACCCTGGCGGAGCTGCCGCAGGACAGGCTGGCGGGCGTTGT
AGTGCGCGACGGCGCGGCAAACTCGCATGCGGCGATTATGGTGCGCGCGCTCGGTATCCCGACCGTCATGGGCGCGGATA
TCCAACCTTCGGTGCTACATCGCCGTACGTTGGTGGTGGATGGCTATCGCGGTGAATTACTGGTCGATCCTGAACCCGTG
CTTATTCAGGAATATCAGCGGCTCATTAGTGAAGAGATAGAACTCAGTCGTCTGGCGGAGGACGACGTCAATCTTCCCGC
CCAACTGAAAAGCGGCGAGCGAGTCAAAGTGATGCTGAATGCCGGGCTCAGTCCCGAGCATGAGGAAAAGTTAGGCAGTC
GGATCGACGGTATCGGCCTGTACCGTACTGAAATTCCCTTTATGCTGCAAAGCGGGTTTCCCTCGGAAGAAGAGCAGGTG
GCGCAGTATCAAGGGATGCTGCAAATGTTTAACGATAAACCGGTGACGCTGCGGACCCTGGATGTGGGAGCGGATAAGCA
ACTGCCCTATATGCCGATCAGCGAGGAGAACCCGTGTCTGGGCTGGCGCGGGATCCGCATTACGCTGGATCAGCCGGAGA
TATTTTTGATCCAGGTCCGCGCGATGCTGCGTGCCAATGCGGCGACTGGCAACCTCAGTATTTTGTTGCCGATGGTCACC
AGTATTGATGAAGTCGATGAAGCGCGGCGTCTGATCGAACGCGCCGGGCGCGAGGTCGAGGAGATGATCGGCTATGCGAT
CCCGAAACCGCGTATCGGCATTATGCTGGAAGTACCGTCAATGGTATTTATGCTGCCGCATCTGGCTAACCGGATCGACT
TTATCTCGGTCGGTACCAACGATTTAACACAATATATCCTGGCGGTAGATCGCAACAACACTCGCGTGGCCAGTATTTAT
GACAGTCTGCATCCGGCTATGCTGCGCGCGCTGTCCATGATTGCACAGGAAGCCGAAAAACATGGTCTCGATCTTCGCTT
GTGCGGCGAGATGGCGGGCGATCCGATGTGCGTGGCGATTCTTATCGGTCTGGGATATCGCCATCTTTCGATGAATGGCC
GTTCGGTAGCGCGTGTGAAATATCTGCTGCGGCATATCGATTTTGAAGACGCGCAAACCCTTGCCAGACGCAGCCTTGAG
GCGCAGATGGCGACCGAGGTGCGTCATCAGGTGGCGGCGTTTATGGAGCGCCGCGGGATGGGGGGATTGATTCGCGGAGG
GTTGTAA

Upstream 100 bases:

>100_bases
GAAAGAGTTTGCAAGTGTTGTCATGGCGCTTCAGGATAATCCCCCTAAGCTACAAAGCGCGCCTGCTTATCGACGTAAAA
GAGGTTAAGCCACGCAAATT

Downstream 100 bases:

>100_bases
CGACTGGAGCGGGAAAAGAGAGTGACTGTGGATTCACGGCGCAGACAACACGCCTGCAAGATGAAAGACAATGCGTATAC
ATATCTTTTAACGGTAATCG

Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKKPRGRTVALAFDEGIVGLVGR
LAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQVT
ALFGQYRQTRIRALPAAPGVAIATGWQDATMPLMEQVYEASTLDTSLERERLTGALEEAANEFRRYSKRFAAGAQKETAA
IFDLYSHLLSDARLRRELFAEVDKGAVAEWAVKKIIEKFAEQFAALTDNYLKERAGDLRTLGQRLLFHLDDSVQGPNAWP
ERFILVADELSATTLAELPQDRLAGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLVVDGYRGELLVDPEPV
LIQEYQRLISEEIELSRLAEDDVNLPAQLKSGERVKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLSILLPMVT
SIDEVDEARRLIERAGREVEEMIGYAIPKPRIGIMLEVPSMVFMLPHLANRIDFISVGTNDLTQYILAVDRNNTRVASIY
DSLHPAMLRALSMIAQEAEKHGLDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARVKYLLRHIDFEDAQTLARRSLE
AQMATEVRHQVAAFMERRGMGGLIRGGL

Sequences:

>Translated_748_residues
MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKKPRGRTVALAFDEGIVGLVGR
LAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQVT
ALFGQYRQTRIRALPAAPGVAIATGWQDATMPLMEQVYEASTLDTSLERERLTGALEEAANEFRRYSKRFAAGAQKETAA
IFDLYSHLLSDARLRRELFAEVDKGAVAEWAVKKIIEKFAEQFAALTDNYLKERAGDLRTLGQRLLFHLDDSVQGPNAWP
ERFILVADELSATTLAELPQDRLAGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLVVDGYRGELLVDPEPV
LIQEYQRLISEEIELSRLAEDDVNLPAQLKSGERVKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLSILLPMVT
SIDEVDEARRLIERAGREVEEMIGYAIPKPRIGIMLEVPSMVFMLPHLANRIDFISVGTNDLTQYILAVDRNNTRVASIY
DSLHPAMLRALSMIAQEAEKHGLDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARVKYLLRHIDFEDAQTLARRSLE
AQMATEVRHQVAAFMERRGMGGLIRGGL
>Mature_748_residues
MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKKPRGRTVALAFDEGIVGLVGR
LAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQVT
ALFGQYRQTRIRALPAAPGVAIATGWQDATMPLMEQVYEASTLDTSLERERLTGALEEAANEFRRYSKRFAAGAQKETAA
IFDLYSHLLSDARLRRELFAEVDKGAVAEWAVKKIIEKFAEQFAALTDNYLKERAGDLRTLGQRLLFHLDDSVQGPNAWP
ERFILVADELSATTLAELPQDRLAGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLVVDGYRGELLVDPEPV
LIQEYQRLISEEIELSRLAEDDVNLPAQLKSGERVKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLSILLPMVT
SIDEVDEARRLIERAGREVEEMIGYAIPKPRIGIMLEVPSMVFMLPHLANRIDFISVGTNDLTQYILAVDRNNTRVASIY
DSLHPAMLRALSMIAQEAEKHGLDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARVKYLLRHIDFEDAQTLARRSLE
AQMATEVRHQVAAFMERRGMGGLIRGGL

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=748, Percent_Identity=94.9197860962567, Blast_Score=1459, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=579, Percent_Identity=34.1968911917098, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI48994992, Length=502, Percent_Identity=32.8685258964143, Blast_Score=271, Evalue=9e-74,
Organism=Escherichia coli, GI1788726, Length=523, Percent_Identity=32.1223709369025, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1787994, Length=408, Percent_Identity=25.7352941176471, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI226510935, Length=207, Percent_Identity=26.5700483091787, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83628; Mature: 83628

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKK
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHEEEEECCCCCEEEEEECCCCCC
PRGRTVALAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQVTALFGQYRQTRIRALPAAPGV
HHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AIATGWQDATMPLMEQVYEASTLDTSLERERLTGALEEAANEFRRYSKRFAAGAQKETAA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IFDLYSHLLSDARLRRELFAEVDKGAVAEWAVKKIIEKFAEQFAALTDNYLKERAGDLRT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LGQRLLFHLDDSVQGPNAWPERFILVADELSATTLAELPQDRLAGVVVRDGAANSHAAIM
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHCCHHHHCEEEEECCCCCCCCEEE
VRALGIPTVMGADIQPSVLHRRTLVVDGYRGELLVDPEPVLIQEYQRLISEEIELSRLAE
EEECCCCCEECCCCCHHHHHCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
DDVNLPAQLKSGERVKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
CCCCCCHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCEEEHHCCCHHHHCCCCCHHHHH
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHHH
AMLRANAATGNLSILLPMVTSIDEVDEARRLIERAGREVEEMIGYAIPKPRIGIMLEVPS
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEECHH
MVFMLPHLANRIDFISVGTNDLTQYILAVDRNNTRVASIYDSLHPAMLRALSMIAQEAEK
HHHHHHHHHCCCEEEECCCHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
HGLDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARVKYLLRHIDFEDAQTLARRSLE
CCCCEEEEHHHCCCHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
AQMATEVRHQVAAFMERRGMGGLIRGGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLADHDRRCYYLMATRGLKK
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHEEEEECCCCCEEEEEECCCCCC
PRGRTVALAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSVKEERFRAFLGVPIIQRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQVTALFGQYRQTRIRALPAAPGV
HHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AIATGWQDATMPLMEQVYEASTLDTSLERERLTGALEEAANEFRRYSKRFAAGAQKETAA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IFDLYSHLLSDARLRRELFAEVDKGAVAEWAVKKIIEKFAEQFAALTDNYLKERAGDLRT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LGQRLLFHLDDSVQGPNAWPERFILVADELSATTLAELPQDRLAGVVVRDGAANSHAAIM
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHCCHHHHCEEEEECCCCCCCCEEE
VRALGIPTVMGADIQPSVLHRRTLVVDGYRGELLVDPEPVLIQEYQRLISEEIELSRLAE
EEECCCCCEECCCCCHHHHHCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
DDVNLPAQLKSGERVKVMLNAGLSPEHEEKLGSRIDGIGLYRTEIPFMLQSGFPSEEEQV
CCCCCCHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCEEEHHCCCHHHHCCCCCHHHHH
AQYQGMLQMFNDKPVTLRTLDVGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHHH
AMLRANAATGNLSILLPMVTSIDEVDEARRLIERAGREVEEMIGYAIPKPRIGIMLEVPS
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEECHH
MVFMLPHLANRIDFISVGTNDLTQYILAVDRNNTRVASIYDSLHPAMLRALSMIAQEAEK
HHHHHHHHHCCCEEEECCCHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
HGLDLRLCGEMAGDPMCVAILIGLGYRHLSMNGRSVARVKYLLRHIDFEDAQTLARRSLE
CCCCEEEEHHHCCCHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
AQMATEVRHQVAAFMERRGMGGLIRGGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]