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The map label for this gene is shiA [H]

Identifier: 194734221

GI number: 194734221

Start: 250369

End: 251670

Strand: Direct

Name: shiA [H]

Synonym: SeSA_A0236

Alternate gene names: 194734221

Gene position: 250369-251670 (Clockwise)

Preceding gene: 194735751

Following gene: 194735516

Centisome position: 5.32

GC content: 50.92

Gene sequence:

>1302_bases
ATGTCCGGAACGTATAATGCTACTATTCGCCGGGTGGTGATTTCCGCCTGGATAGGAAACTCCATTGAATATTATGATTT
TCTATTATACGGCCTGGCAAGCGCGCTGGTATTTGGCCCGCTTTTTTTCCCTGGCGCCAGCCCATTGACTGCAACGTTAT
CTTCTTTTGCCAGTTTCGGCGTGGGATTTATTTCCCGGCCGCTGGGCGCGCTATTTTTTGGCAACAGGGGAGATACGCTT
GGGCGTAAAAACACGTTATTAATCACGTTAGGTGGGATGGGCGCGGTCACGTTTCTCATCGGTTGCCTGCCTTCTTACGC
CAGTATTGGCGCGCTGGCGCCTGCCTTACTGGTTATTCTGCGTTTTCTGCAGGGATTTATGGTTGGAGGCGAATGGGGAG
GCGCCATGTTAATGGTCGTGGAATATGCTGCTGGAAAGCACCGGGGGCGGTTAAGCGCGCTATCGCAAACAGGAGGACTC
ACGGGACAATTATTGGCGACAGGCGCATTTATAGTCGTGACCCAATTACCGAAGGATGCGTTGTTATTGTGGGGATGGCG
TATTCCTTTTCTGCTCAGTGCGCTTTTGGTGCTGCCTGGATTATATATGCGTCATCGTCTGGATGAGACGCCTGTCTTCC
GTGCGTTTAAAAAGCAGCAGGCCATTAAGCATATGCAGCAAAAAGAAGAAAGGCCGGTGGTCAAAGTGGTACGAGAACAA
TGGCGCAGCATTTTGCTGATTATAATTCTACGTTTTGCGGAAAGCGTTCCCTTTTTTCTTGCCACCGTCTTCGCGGTTTC
CTGGGCGACGACGCAGCTTGGGATTGCCAGCCTGACTATTCTGTACATTGTAATGTTCACCTGTCTGCTGGCGTATCCCA
TGCACGTGCTATTTGGCATCATGTCGGATCGGCGTGGCTGCCGTCAGGTGTATATTTTCGGCGCGCTTTTTGTCGCGGCA
ATGGCTTTTCCCTTTTTCTGGCTACTGGAAAGCCGTTCGCTCATACTGATGACAATGGGCTACGTTTTGCTTATTAACAT
TGGTCATAATTCACTGAATGCCGTGCAACCTTCTTTTTTCGCCGGACTGTTTCATCCGCCTGTACGCTACAGCGGTTCAT
CAATTGGCGCTCAGTTGGGGGCGGTTGTGGCAGGGGGATTCACGCCGTTTATCGCTAAAGCGTTAAGCGCAGTCTATGAT
AACAGTTGGACTTTGGTTGCCGGTTATGTCGTTTTGACGGCGTTGGCTTCGGCATTTGCGGCAAAGATTGCGCCGGAAAC
CGTGTTACCGCATTCGCCGTAA

Upstream 100 bases:

>100_bases
AAGTAATTCAACGCGATATTCCGTTTATAGAGAAAGCGTTTGAGGTCAGTGTGGAAAATTGACCGGAGGTAGTGGTCGAT
AACCTTGAGAGGCGGAGTCT

Downstream 100 bases:

>100_bases
TGCAAAGGGGGGCTGTCGCCCCCGCTTTTCACTCGCTTAATCAATAGTGCGCAATAATTCGTTAATGCCCACTTTGCCAC
GCGTTTTGGCGTCGACTTTT

Product: shikimate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSGTYNATIRRVVISAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFGVGFISRPLGALFFGNRGDTL
GRKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVILRFLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGL
TGQLLATGAFIVVTQLPKDALLLWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAIKHMQQKEERPVVKVVREQ
WRSILLIIILRFAESVPFFLATVFAVSWATTQLGIASLTILYIVMFTCLLAYPMHVLFGIMSDRRGCRQVYIFGALFVAA
MAFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQPSFFAGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYD
NSWTLVAGYVVLTALASAFAAKIAPETVLPHSP

Sequences:

>Translated_433_residues
MSGTYNATIRRVVISAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFGVGFISRPLGALFFGNRGDTL
GRKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVILRFLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGL
TGQLLATGAFIVVTQLPKDALLLWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAIKHMQQKEERPVVKVVREQ
WRSILLIIILRFAESVPFFLATVFAVSWATTQLGIASLTILYIVMFTCLLAYPMHVLFGIMSDRRGCRQVYIFGALFVAA
MAFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQPSFFAGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYD
NSWTLVAGYVVLTALASAFAAKIAPETVLPHSP
>Mature_432_residues
SGTYNATIRRVVISAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFGVGFISRPLGALFFGNRGDTLG
RKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVILRFLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGLT
GQLLATGAFIVVTQLPKDALLLWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAIKHMQQKEERPVVKVVREQW
RSILLIIILRFAESVPFFLATVFAVSWATTQLGIASLTILYIVMFTCLLAYPMHVLFGIMSDRRGCRQVYIFGALFVAAM
AFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQPSFFAGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYDN
SWTLVAGYVVLTALASAFAAKIAPETVLPHSP

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=419, Percent_Identity=41.0501193317422, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1790550, Length=408, Percent_Identity=33.8235294117647, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789941, Length=399, Percent_Identity=33.5839598997494, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1788942, Length=405, Percent_Identity=30.3703703703704, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87082404, Length=403, Percent_Identity=24.317617866005, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46973; Mature: 46842

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTYNATIRRVVISAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFG
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VGFISRPLGALFFGNRGDTLGRKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVIL
HHHHHCCHHHHEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGLTGQLLATGAFIVVTQLPKDA
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHCCEEEEEEECCCHH
LLLWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAIKHMQQKEERPVVKVVREQ
HHHHCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
WRSILLIIILRFAESVPFFLATVFAVSWATTQLGIASLTILYIVMFTCLLAYPMHVLFGI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSDRRGCRQVYIFGALFVAAMAFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQPSFF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCCCCCCCHHHH
AGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYDNSWTLVAGYVVLTALASAFA
HHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AKIAPETVLPHSP
HHCCCCCCCCCCC
>Mature Secondary Structure 
SGTYNATIRRVVISAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFG
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VGFISRPLGALFFGNRGDTLGRKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVIL
HHHHHCCHHHHEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGLTGQLLATGAFIVVTQLPKDA
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHCCEEEEEEECCCHH
LLLWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAIKHMQQKEERPVVKVVREQ
HHHHCCHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
WRSILLIIILRFAESVPFFLATVFAVSWATTQLGIASLTILYIVMFTCLLAYPMHVLFGI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSDRRGCRQVYIFGALFVAAMAFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQPSFF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCCCCCCCHHHH
AGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYDNSWTLVAGYVVLTALASAFA
HHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AKIAPETVLPHSP
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]