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The map label for this gene is yuxG [H]

Identifier: 194334602

GI number: 194334602

Start: 1968951

End: 1971068

Strand: Reverse

Name: yuxG [H]

Synonym: Paes_1803

Alternate gene names: 194334602

Gene position: 1971068-1968951 (Counterclockwise)

Preceding gene: 194334603

Following gene: 194334601

Centisome position: 78.44

GC content: 54.3

Gene sequence:

>2118_bases
ATGCAAAACCTCTGGGATACTTCAGGCTGCGTCTCATGTATCGAAGCGCTTTGTTCGGGGACCGAAATACCTCGTGAGCT
CTGTGAACTTGTCTATGCGTCACGTCTTCTCGGTCGCGAAAACAGGCTGGTCATGCATGGCGGAGGTAATACATCGGTCA
AAAGCGAGCTTACCGATGTTCTGGGCAACAAGGCCAATGTGCTGTTTATCAAAGGCAGCGGAGTTGATCTCAAAGAGATT
GTTCCGCACGATTTTACTCCTGTCCGGCTTGAGCCGATGCAGAGATTGCTCAAGCTGATCCACGAGGGGAGGGGCCATAG
CGAAGAGGACCTTCGTCGTTTTTCCAACAAGGAGTTCAAGAACTTTCTTTTTTTGAACCTGTTCAGCCTGAACGACAATA
TGGTGCAGAAGGGTTTGACGCCATCGATCGAGACCCTCTTGCATGCATTCCTTCCCCATCGCTATATTTTCCATACTCAT
GCACAGGCATTGCTCACCTTGAGCAATCAGCCGGATGGTGAAGCGCTCTGCAGGGAGGTGCTTGGTGATCGTATGGGTAT
TGTGCCCTATATCCGGCCCGGTATCGAGCTTGCATGCTCTGCCGAGCGGGTCTATGCCAACCACGCTGACATAGAAGGCC
TGGTGCTGCTCAAACACGGTCTGGTGACGTTCGGCGAGAGTGCAAAAGATGCCTACGATCGAATGATCTCTGCTATCAGC
ACAATGGAGGATCGCATCGACAGGGCCGGTCGGAAGGTGTTTGCGCAGACGGAACTTCCCGGATCGCTGATGGCGGTCGA
GCATGTCGCTCCGGTTATTCGCGGTGCATGCGTTCAGGAACGCACGCCACGTAGCAGAGACTACGATACGTTTATTCTCG
ACTTCCGTACGTCGCCCGATATTATCGAATATGTCAACAGAGCCGATCTGGCACAACTCAGCGCCAAAGGCGCTATGACC
CCTGACTTCATCATCAGGACCAAGAACCGGCCTCTCGTGGTTCCGGCACCTGATGCTGCCGATCCCGATGCATTCCGTCG
CGATGTGCATGAGGCTGTTGACGCCTATAAAAAAGAGTATACAGCCTATTTCGAGCGCCAGAAGGCGGCGAGCGGCATGG
ACGTCGAGATGCTCGACCCGCTGCCCAGAGTTGCGCTGGTTCCCGGTCTCGGGCTTTTCGGTCTGGGCAGGACTCTTCGC
GACGCCCGCGTGAATGCCGATATCGCAGAAAGTTCTGCCGAAGCGGTGCTGAAGGCCGAAAGCGTTGGTTCCTTCGAGTC
CATCAGTGAAAAAGAGGTGTTCGAGATTGAGTATTGGGAGATGGAGCAGGCCAAAGTCAAAAAAGTCCGTCACGATGTCT
TTGCAGGCCGTGTTGCAATGGTTACCGGCGCTGCAGGCGGGATCGGTCTTGCGACAGCCAAAGCATTCAGGGAAAAAGGC
GCTGAAATTGTCATCCTGGACCTCAATCAGGAATCCCTTGATAAAGCGTGTGAAGAGATCGGCCCCGACACGCTGGCCAT
TGCATGTGACGTCACCGATCGAAGCGCGATTGCCGAAGCCTTCAACCGCACGTGTCGGATGTTCGGCGGGATTGATATTG
TTGTTTCCAATGTAGGCATCGCCATTCAGGGCAGGATCGGCGATGTCGACGAAGCGCTTCTTCGCCGGAGTTTCGAGTTG
AATTTCTTTTCTCATCAGACTATTTCACAGCACGCTGTTGCTGTCATGCGACGGCAGGGTATCGGCGGTAACCTGCTCTA
CAACGTTTCAAAACAGGCTGTCAATCCCGGACCGGATTTTGGAGCATACGGACTGCCAAAGGCTGCGACCCTCTTTCTCG
TTCGCCAGTATGCGCTTGATCACGGCAGGGACGGTATCAGGGCAAACGGCATCAATGCCGATCGTATCCGTACCGGTCTT
CTCAATGAAGAGATGATCGCAAAGCGCTCGAAAGCCAGGGGACTGAGCCCTGAAGAGTATATGGCAGGCAACCTTCTCAA
GCTCGAGGTGACGGCTGAAGATGTCGCCCAGGCGTTCGTCCACCTTGCGCTCGAGCAGAAGACAACCGGTTCGATCACCA
CCGTTGATGGCGGCAACATTGCAGCAGCCCTCAGATAA

Upstream 100 bases:

>100_bases
AATGTGTATCGTCATGCCTTGGTCCTGAAAAGGCTGAGGAGCTCATAGAGCTTTTACTTTATAATCGACACCGGCGACGG
TGTGAGAAGTTACAATAGTC

Downstream 100 bases:

>100_bases
CGCCCATAGACCTAACGATTTCAACAACTCAAAAGCGGAGCTACACTATGCCAGAAGCTGATCAGGGAAAACAGGAAAAA
AATTATCACACCGACGTCCC

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MQNLWDTSGCVSCIEALCSGTEIPRELCELVYASRLLGRENRLVMHGGGNTSVKSELTDVLGNKANVLFIKGSGVDLKEI
VPHDFTPVRLEPMQRLLKLIHEGRGHSEEDLRRFSNKEFKNFLFLNLFSLNDNMVQKGLTPSIETLLHAFLPHRYIFHTH
AQALLTLSNQPDGEALCREVLGDRMGIVPYIRPGIELACSAERVYANHADIEGLVLLKHGLVTFGESAKDAYDRMISAIS
TMEDRIDRAGRKVFAQTELPGSLMAVEHVAPVIRGACVQERTPRSRDYDTFILDFRTSPDIIEYVNRADLAQLSAKGAMT
PDFIIRTKNRPLVVPAPDAADPDAFRRDVHEAVDAYKKEYTAYFERQKAASGMDVEMLDPLPRVALVPGLGLFGLGRTLR
DARVNADIAESSAEAVLKAESVGSFESISEKEVFEIEYWEMEQAKVKKVRHDVFAGRVAMVTGAAGGIGLATAKAFREKG
AEIVILDLNQESLDKACEEIGPDTLAIACDVTDRSAIAEAFNRTCRMFGGIDIVVSNVGIAIQGRIGDVDEALLRRSFEL
NFFSHQTISQHAVAVMRRQGIGGNLLYNVSKQAVNPGPDFGAYGLPKAATLFLVRQYALDHGRDGIRANGINADRIRTGL
LNEEMIAKRSKARGLSPEEYMAGNLLKLEVTAEDVAQAFVHLALEQKTTGSITTVDGGNIAAALR

Sequences:

>Translated_705_residues
MQNLWDTSGCVSCIEALCSGTEIPRELCELVYASRLLGRENRLVMHGGGNTSVKSELTDVLGNKANVLFIKGSGVDLKEI
VPHDFTPVRLEPMQRLLKLIHEGRGHSEEDLRRFSNKEFKNFLFLNLFSLNDNMVQKGLTPSIETLLHAFLPHRYIFHTH
AQALLTLSNQPDGEALCREVLGDRMGIVPYIRPGIELACSAERVYANHADIEGLVLLKHGLVTFGESAKDAYDRMISAIS
TMEDRIDRAGRKVFAQTELPGSLMAVEHVAPVIRGACVQERTPRSRDYDTFILDFRTSPDIIEYVNRADLAQLSAKGAMT
PDFIIRTKNRPLVVPAPDAADPDAFRRDVHEAVDAYKKEYTAYFERQKAASGMDVEMLDPLPRVALVPGLGLFGLGRTLR
DARVNADIAESSAEAVLKAESVGSFESISEKEVFEIEYWEMEQAKVKKVRHDVFAGRVAMVTGAAGGIGLATAKAFREKG
AEIVILDLNQESLDKACEEIGPDTLAIACDVTDRSAIAEAFNRTCRMFGGIDIVVSNVGIAIQGRIGDVDEALLRRSFEL
NFFSHQTISQHAVAVMRRQGIGGNLLYNVSKQAVNPGPDFGAYGLPKAATLFLVRQYALDHGRDGIRANGINADRIRTGL
LNEEMIAKRSKARGLSPEEYMAGNLLKLEVTAEDVAQAFVHLALEQKTTGSITTVDGGNIAAALR
>Mature_705_residues
MQNLWDTSGCVSCIEALCSGTEIPRELCELVYASRLLGRENRLVMHGGGNTSVKSELTDVLGNKANVLFIKGSGVDLKEI
VPHDFTPVRLEPMQRLLKLIHEGRGHSEEDLRRFSNKEFKNFLFLNLFSLNDNMVQKGLTPSIETLLHAFLPHRYIFHTH
AQALLTLSNQPDGEALCREVLGDRMGIVPYIRPGIELACSAERVYANHADIEGLVLLKHGLVTFGESAKDAYDRMISAIS
TMEDRIDRAGRKVFAQTELPGSLMAVEHVAPVIRGACVQERTPRSRDYDTFILDFRTSPDIIEYVNRADLAQLSAKGAMT
PDFIIRTKNRPLVVPAPDAADPDAFRRDVHEAVDAYKKEYTAYFERQKAASGMDVEMLDPLPRVALVPGLGLFGLGRTLR
DARVNADIAESSAEAVLKAESVGSFESISEKEVFEIEYWEMEQAKVKKVRHDVFAGRVAMVTGAAGGIGLATAKAFREKG
AEIVILDLNQESLDKACEEIGPDTLAIACDVTDRSAIAEAFNRTCRMFGGIDIVVSNVGIAIQGRIGDVDEALLRRSFEL
NFFSHQTISQHAVAVMRRQGIGGNLLYNVSKQAVNPGPDFGAYGLPKAATLFLVRQYALDHGRDGIRANGINADRIRTGL
LNEEMIAKRSKARGLSPEEYMAGNLLKLEVTAEDVAQAFVHLALEQKTTGSITTVDGGNIAAALR

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG3347

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=193, Percent_Identity=29.0155440414508, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI126723191, Length=202, Percent_Identity=26.7326732673267, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI31542939, Length=203, Percent_Identity=29.5566502463054, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI7705925, Length=250, Percent_Identity=28.8, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI224922801, Length=147, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI5031737, Length=193, Percent_Identity=27.979274611399, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI59889578, Length=235, Percent_Identity=31.4893617021277, Blast_Score=67, Evalue=7e-11,
Organism=Homo sapiens, GI33667109, Length=195, Percent_Identity=28.7179487179487, Blast_Score=67, Evalue=8e-11,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=30.4347826086957, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87082100, Length=255, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789378, Length=191, Percent_Identity=29.8429319371728, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786812, Length=259, Percent_Identity=28.1853281853282, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=190, Percent_Identity=32.6315789473684, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI193204405, Length=201, Percent_Identity=32.3383084577114, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17562906, Length=200, Percent_Identity=33.5, Blast_Score=86, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17531453, Length=210, Percent_Identity=31.4285714285714, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17560220, Length=202, Percent_Identity=32.6732673267327, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=200, Percent_Identity=31.5, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17544670, Length=272, Percent_Identity=28.3088235294118, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=265, Percent_Identity=29.4339622641509, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=205, Percent_Identity=27.3170731707317, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17560150, Length=199, Percent_Identity=27.6381909547739, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=223, Percent_Identity=28.6995515695067, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=199, Percent_Identity=28.1407035175879, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17563726, Length=202, Percent_Identity=27.2277227722772, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=186, Percent_Identity=28.494623655914, Blast_Score=66, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322227, Length=237, Percent_Identity=25.7383966244726, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21355319, Length=224, Percent_Identity=29.9107142857143, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24663911, Length=205, Percent_Identity=25.8536585365854, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI28571387, Length=243, Percent_Identity=24.6913580246914, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24641232, Length=239, Percent_Identity=25.1046025104602, Blast_Score=68, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR013454
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short; PF00596 Aldolase_II [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 77424; Mature: 77424

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLWDTSGCVSCIEALCSGTEIPRELCELVYASRLLGRENRLVMHGGGNTSVKSELTDV
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
LGNKANVLFIKGSGVDLKEIVPHDFTPVRLEPMQRLLKLIHEGRGHSEEDLRRFSNKEFK
HCCCCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHH
NFLFLNLFSLNDNMVQKGLTPSIETLLHAFLPHRYIFHTHAQALLTLSNQPDGEALCREV
HEEEEEEEECCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCHHHHHHHH
LGDRMGIVPYIRPGIELACSAERVYANHADIEGLVLLKHGLVTFGESAKDAYDRMISAIS
HCCCCCCCCCCCCCCCEEECHHHHHCCCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHHH
TMEDRIDRAGRKVFAQTELPGSLMAVEHVAPVIRGACVQERTPRSRDYDTFILDFRTSPD
HHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCH
IIEYVNRADLAQLSAKGAMTPDFIIRTKNRPLVVPAPDAADPDAFRRDVHEAVDAYKKEY
HHHHHCHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
TAYFERQKAASGMDVEMLDPLPRVALVPGLGLFGLGRTLRDARVNADIAESSAEAVLKAE
HHHHHHHHHCCCCCCHHHCCCCCHHHHCCCCHHHHCHHHHHHHCCCHHHHHHHHHHHHHH
SVGSFESISEKEVFEIEYWEMEQAKVKKVRHDVFAGRVAMVTGAAGGIGLATAKAFREKG
CCCCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCC
AEIVILDLNQESLDKACEEIGPDTLAIACDVTDRSAIAEAFNRTCRMFGGIDIVVSNVGI
CEEEEEECCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCE
AIQGRIGDVDEALLRRSFELNFFSHQTISQHAVAVMRRQGIGGNLLYNVSKQAVNPGPDF
EEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCCC
GAYGLPKAATLFLVRQYALDHGRDGIRANGINADRIRTGLLNEEMIAKRSKARGLSPEEY
CCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHCCCCHHHHHHHHHHCCCCHHHH
MAGNLLKLEVTAEDVAQAFVHLALEQKTTGSITTVDGGNIAAALR
HCCCEEEEEECHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECC
>Mature Secondary Structure
MQNLWDTSGCVSCIEALCSGTEIPRELCELVYASRLLGRENRLVMHGGGNTSVKSELTDV
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
LGNKANVLFIKGSGVDLKEIVPHDFTPVRLEPMQRLLKLIHEGRGHSEEDLRRFSNKEFK
HCCCCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHH
NFLFLNLFSLNDNMVQKGLTPSIETLLHAFLPHRYIFHTHAQALLTLSNQPDGEALCREV
HEEEEEEEECCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCHHHHHHHH
LGDRMGIVPYIRPGIELACSAERVYANHADIEGLVLLKHGLVTFGESAKDAYDRMISAIS
HCCCCCCCCCCCCCCCEEECHHHHHCCCCCCCEEEEEECCHHHCCCCHHHHHHHHHHHHH
TMEDRIDRAGRKVFAQTELPGSLMAVEHVAPVIRGACVQERTPRSRDYDTFILDFRTSPD
HHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCH
IIEYVNRADLAQLSAKGAMTPDFIIRTKNRPLVVPAPDAADPDAFRRDVHEAVDAYKKEY
HHHHHCHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
TAYFERQKAASGMDVEMLDPLPRVALVPGLGLFGLGRTLRDARVNADIAESSAEAVLKAE
HHHHHHHHHCCCCCCHHHCCCCCHHHHCCCCHHHHCHHHHHHHCCCHHHHHHHHHHHHHH
SVGSFESISEKEVFEIEYWEMEQAKVKKVRHDVFAGRVAMVTGAAGGIGLATAKAFREKG
CCCCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCC
AEIVILDLNQESLDKACEEIGPDTLAIACDVTDRSAIAEAFNRTCRMFGGIDIVVSNVGI
CEEEEEECCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCE
AIQGRIGDVDEALLRRSFELNFFSHQTISQHAVAVMRRQGIGGNLLYNVSKQAVNPGPDF
EEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCCCCC
GAYGLPKAATLFLVRQYALDHGRDGIRANGINADRIRTGLLNEEMIAKRSKARGLSPEEY
CCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHCCCCHHHHHHHHHHCCCCHHHH
MAGNLLKLEVTAEDVAQAFVHLALEQKTTGSITTVDGGNIAAALR
HCCCEEEEEECHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 8188684 [H]