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The map label for this gene is mrdA [H]

Identifier: 194333543

GI number: 194333543

Start: 755213

End: 757135

Strand: Reverse

Name: mrdA [H]

Synonym: Paes_0709

Alternate gene names: 194333543

Gene position: 757135-755213 (Counterclockwise)

Preceding gene: 194333544

Following gene: 194333542

Centisome position: 30.13

GC content: 50.18

Gene sequence:

>1923_bases
ATGGATAAAATTCAGCAAAGAACACTGAACGTGTCATTGATTGTCATTGCTGTTTTTGTCGCTCTCTTTGCCCGTCTGGT
CTATCTGCAGGTTTTTGAGTACAAGGAACTCGGTTCGATTTCCGATGCAAACAGTCTCCGCAGAATCTGGGTTCAACCGC
CGAGAGGCCGTATGATCGACAGAAACGGCGACATTATCGTCACCAATCAGCCACTCTACACGATCAAGGTGATTCCTTCG
GAGTTCAGCGACGAGAGAAAGGAACTCATGGCCCTGCTGTTAAACATCAACCCGGACGAACTGGAATCGAGAATTCAGAA
AGGATATCGATACAACCGCTTCGCTCCCGTTGTCGTCGGCAGAGATATCGCTCAGGAGGATATGATGCGCCTGAATGAAA
ATCTCTGGCAGCTACCCGGAGTGCTCATCGAAGTTGAGAACAAAAGAAAATATTCCAGTCCGGTCAATGCGTCACATATC
CTCGGCTACCTGAACCTCATTTCAAAAAAGCAGCTGGAAACCATGGCCGACAAAGGCTATACGCCTGATGATAAAACCGG
AAGCAAGGGACTCGAAAAATTTTATGAAGAACCGTTACGAGGCAGCAAAGGAGTCCGATACGAACTGGTCAACTCCGTTG
GAAGAGTTGTCGGCAAGTTTGAGAATGGTCAAAAAGACGAACCCTTCATCAATGGCAACGACCTCTACCTGACACTGGAC
ACCGGGCTTCAGGAGCTTGCCGAGACGCTTCTGAAAAAGACAGAAAAATCCGGTGCTGTCGTCGCTATAGACCCGAACTC
CGGAGGCATTCTGGCTATGGCCAGCGAACCGGATTACGATCTTGAAATCCTTAACGGGAGAACCGACAGTAAAGAATGGA
GCAAAATTGTACGCGACCCCAGAAAACCTCTTTTCAACCGCGCTATTCAGGCAACATATCCTCCTGGATCCATCTACAAG
CTGCTCCTTGCTATCGCAGGACTTGAAACAAAGGCCATCAGTCCTGACAAAAGGATCTTCTGCAGCGGGGTGTTCAGGTT
GGGAAGAGGCCGCTTTCTCTGCCATGGTGGACAAGGGCACGGATCAGTCGACATGAAAAAAGCTATCATAGAATCATGCA
ATACCTACTTCTACAACCTGATTTTCGATGTCGGTTTTGAAAAATGGTCAGAATACGGCAGAATGTTCGGGTTTGGCAAA
ACAACAGGCCTCGACCTTCCCGGCGAACGTTCAGCCCCGCTCCCCTCACCTGAATACTACGACAAGCGATACGGCAAAGG
CAAATGGACAAAAGGATACCTTGTCAGCCTTTCCATCGGTCAGGGAGAGCTTGGAACAACACCGCTCCAGCTTGCCGCAT
TTACAGCGACAATCGCAAACAGAGGGACCTACTATCAGCCTCACCTGGTCAAGGGGTACCGCGAGGCAAAAAACGACAAC
TATGTCCAACTCGACTTCTCGGAAGAGAAACTGCCTGTATCCGAAGAAACCTTTGAGTTCATTGCTGAATCAATGGCTGG
CGTTGTCGAGAAAGGCACAGGGCGGCTCGCAGCCATTGATGGCATAAGAGTGGCAGGAAAAACCGGCACGGCGCAGAACC
CTCACGGCAAAGACCATGCATGGTTTGTCGCATTTGCTCCAGTTGAAAAACCGGTGATCGCCGTAGCTGTTCTGGTAGAA
AATGCCGGCTATGGCGGTAGCATCTCCGCGCCGATAGCGCGAGACATTATCGATTTATACATCAACGGGCCCGACACAAC
AGCAACTGCAGTCGATTCAACCGCAGCCATAACGTCCGACAGAGCCACACCAGCCGCAACTCCCGCTCGGATAATTGCCA
CGCAAAACGTTCAGGATTCGGCGCTGACGGCGATCCCCGACAGTCTGAAAAAGCTCAACAACAACGAGACGAATGCTTTT
TAA

Upstream 100 bases:

>100_bases
CCTTTTGTATACGGTCGGTGTTGCGACACTGATGAATATGATCATAGCCGTCATAGCCTACAGGCTTCTTTTAAGAAATA
CTTTTATTCTCAACTGACGA

Downstream 100 bases:

>100_bases
AACAGATCCATCGCAAATACAGCGATTTCTTGAAGACACCAGCAATATCAGAACAGGCTCTACCCCGGGAGTCTACTTCC
CGCAAAACGCCGATGAGGTC

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MDKIQQRTLNVSLIVIAVFVALFARLVYLQVFEYKELGSISDANSLRRIWVQPPRGRMIDRNGDIIVTNQPLYTIKVIPS
EFSDERKELMALLLNINPDELESRIQKGYRYNRFAPVVVGRDIAQEDMMRLNENLWQLPGVLIEVENKRKYSSPVNASHI
LGYLNLISKKQLETMADKGYTPDDKTGSKGLEKFYEEPLRGSKGVRYELVNSVGRVVGKFENGQKDEPFINGNDLYLTLD
TGLQELAETLLKKTEKSGAVVAIDPNSGGILAMASEPDYDLEILNGRTDSKEWSKIVRDPRKPLFNRAIQATYPPGSIYK
LLLAIAGLETKAISPDKRIFCSGVFRLGRGRFLCHGGQGHGSVDMKKAIIESCNTYFYNLIFDVGFEKWSEYGRMFGFGK
TTGLDLPGERSAPLPSPEYYDKRYGKGKWTKGYLVSLSIGQGELGTTPLQLAAFTATIANRGTYYQPHLVKGYREAKNDN
YVQLDFSEEKLPVSEETFEFIAESMAGVVEKGTGRLAAIDGIRVAGKTGTAQNPHGKDHAWFVAFAPVEKPVIAVAVLVE
NAGYGGSISAPIARDIIDLYINGPDTTATAVDSTAAITSDRATPAATPARIIATQNVQDSALTAIPDSLKKLNNNETNAF

Sequences:

>Translated_640_residues
MDKIQQRTLNVSLIVIAVFVALFARLVYLQVFEYKELGSISDANSLRRIWVQPPRGRMIDRNGDIIVTNQPLYTIKVIPS
EFSDERKELMALLLNINPDELESRIQKGYRYNRFAPVVVGRDIAQEDMMRLNENLWQLPGVLIEVENKRKYSSPVNASHI
LGYLNLISKKQLETMADKGYTPDDKTGSKGLEKFYEEPLRGSKGVRYELVNSVGRVVGKFENGQKDEPFINGNDLYLTLD
TGLQELAETLLKKTEKSGAVVAIDPNSGGILAMASEPDYDLEILNGRTDSKEWSKIVRDPRKPLFNRAIQATYPPGSIYK
LLLAIAGLETKAISPDKRIFCSGVFRLGRGRFLCHGGQGHGSVDMKKAIIESCNTYFYNLIFDVGFEKWSEYGRMFGFGK
TTGLDLPGERSAPLPSPEYYDKRYGKGKWTKGYLVSLSIGQGELGTTPLQLAAFTATIANRGTYYQPHLVKGYREAKNDN
YVQLDFSEEKLPVSEETFEFIAESMAGVVEKGTGRLAAIDGIRVAGKTGTAQNPHGKDHAWFVAFAPVEKPVIAVAVLVE
NAGYGGSISAPIARDIIDLYINGPDTTATAVDSTAAITSDRATPAATPARIIATQNVQDSALTAIPDSLKKLNNNETNAF
>Mature_640_residues
MDKIQQRTLNVSLIVIAVFVALFARLVYLQVFEYKELGSISDANSLRRIWVQPPRGRMIDRNGDIIVTNQPLYTIKVIPS
EFSDERKELMALLLNINPDELESRIQKGYRYNRFAPVVVGRDIAQEDMMRLNENLWQLPGVLIEVENKRKYSSPVNASHI
LGYLNLISKKQLETMADKGYTPDDKTGSKGLEKFYEEPLRGSKGVRYELVNSVGRVVGKFENGQKDEPFINGNDLYLTLD
TGLQELAETLLKKTEKSGAVVAIDPNSGGILAMASEPDYDLEILNGRTDSKEWSKIVRDPRKPLFNRAIQATYPPGSIYK
LLLAIAGLETKAISPDKRIFCSGVFRLGRGRFLCHGGQGHGSVDMKKAIIESCNTYFYNLIFDVGFEKWSEYGRMFGFGK
TTGLDLPGERSAPLPSPEYYDKRYGKGKWTKGYLVSLSIGQGELGTTPLQLAAFTATIANRGTYYQPHLVKGYREAKNDN
YVQLDFSEEKLPVSEETFEFIAESMAGVVEKGTGRLAAIDGIRVAGKTGTAQNPHGKDHAWFVAFAPVEKPVIAVAVLVE
NAGYGGSISAPIARDIIDLYINGPDTTATAVDSTAAITSDRATPAATPARIIATQNVQDSALTAIPDSLKKLNNNETNAF

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=625, Percent_Identity=30.24, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1786272, Length=588, Percent_Identity=27.2108843537415, Blast_Score=148, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 70740; Mature: 70740

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIQQRTLNVSLIVIAVFVALFARLVYLQVFEYKELGSISDANSLRRIWVQPPRGRMID
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCEEC
RNGDIIVTNQPLYTIKVIPSEFSDERKELMALLLNINPDELESRIQKGYRYNRFAPVVVG
CCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEC
RDIAQEDMMRLNENLWQLPGVLIEVENKRKYSSPVNASHILGYLNLISKKQLETMADKGY
CHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
TPDDKTGSKGLEKFYEEPLRGSKGVRYELVNSVGRVVGKFENGQKDEPFINGNDLYLTLD
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEEC
TGLQELAETLLKKTEKSGAVVAIDPNSGGILAMASEPDYDLEILNGRTDSKEWSKIVRDP
HHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHCC
RKPLFNRAIQATYPPGSIYKLLLAIAGLETKAISPDKRIFCSGVFRLGRGRFLCHGGQGH
CHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCC
GSVDMKKAIIESCNTYFYNLIFDVGFEKWSEYGRMFGFGKTTGLDLPGERSAPLPSPEYY
CCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
DKRYGKGKWTKGYLVSLSIGQGELGTTPLQLAAFTATIANRGTYYQPHLVKGYREAKNDN
HHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
YVQLDFSEEKLPVSEETFEFIAESMAGVVEKGTGRLAAIDGIRVAGKTGTAQNPHGKDHA
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCCCCCCCCE
WFVAFAPVEKPVIAVAVLVENAGYGGSISAPIARDIIDLYINGPDTTATAVDSTAAITSD
EEEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHCEEEEEECCCCCCHHHCCCHHHCCCC
RATPAATPARIIATQNVQDSALTAIPDSLKKLNNNETNAF
CCCCCCCCEEEEEECCCCCHHHHHCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MDKIQQRTLNVSLIVIAVFVALFARLVYLQVFEYKELGSISDANSLRRIWVQPPRGRMID
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCEEC
RNGDIIVTNQPLYTIKVIPSEFSDERKELMALLLNINPDELESRIQKGYRYNRFAPVVVG
CCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEC
RDIAQEDMMRLNENLWQLPGVLIEVENKRKYSSPVNASHILGYLNLISKKQLETMADKGY
CHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
TPDDKTGSKGLEKFYEEPLRGSKGVRYELVNSVGRVVGKFENGQKDEPFINGNDLYLTLD
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEEC
TGLQELAETLLKKTEKSGAVVAIDPNSGGILAMASEPDYDLEILNGRTDSKEWSKIVRDP
HHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHCC
RKPLFNRAIQATYPPGSIYKLLLAIAGLETKAISPDKRIFCSGVFRLGRGRFLCHGGQGH
CHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCC
GSVDMKKAIIESCNTYFYNLIFDVGFEKWSEYGRMFGFGKTTGLDLPGERSAPLPSPEYY
CCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
DKRYGKGKWTKGYLVSLSIGQGELGTTPLQLAAFTATIANRGTYYQPHLVKGYREAKNDN
HHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
YVQLDFSEEKLPVSEETFEFIAESMAGVVEKGTGRLAAIDGIRVAGKTGTAQNPHGKDHA
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCCCCCCCCE
WFVAFAPVEKPVIAVAVLVENAGYGGSISAPIARDIIDLYINGPDTTATAVDSTAAITSD
EEEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHCEEEEEECCCCCCHHHCCCHHHCCCC
RATPAATPARIIATQNVQDSALTAIPDSLKKLNNNETNAF
CCCCCCCCEEEEEECCCCCHHHHHCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]