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The map label for this gene is dfrA [H]

Identifier: 194333519

GI number: 194333519

Start: 729401

End: 730447

Strand: Reverse

Name: dfrA [H]

Synonym: Paes_0685

Alternate gene names: 194333519

Gene position: 730447-729401 (Counterclockwise)

Preceding gene: 194333520

Following gene: 194333512

Centisome position: 29.07

GC content: 52.91

Gene sequence:

>1047_bases
ATGGCACAACATCCAATCGTCTGCGTCACCGGCGCTACTGGTTTTATTGCAGCCCATCTGATCAAAAAACTGCTGCAACA
GGGCTACGCTGTACGGGGAACCGTCAGGAAACTTTCAAAACACAGCAGTTACCGGTTTCTTGAAAACCTCGACGGAGCGG
CAGAACGCCTGGAGCTCATCGAGGCCGATCTGCTCTCCCCCGGCTCTTTCGATGCAGCCATCGGGGGAACGCAATATGTC
ATGCACACGGCAAGTCCTTACCTGATAAACGTCAGAGACCCGCAAAAAAACCTCGTAGAACCTGCACTGGAGGGAACACG
CTCGGTGCTCGAATCATGCAGAAAAGCCTCAAGCGTCAAACGCGTCGTGCTCACCTCATCAATCGCCGCTATCACCGGCG
CACCGGACAGTAACAGGGTCTTCACTGAAAATGACTGGAACACAACCTCCTCGCTGAAAGAAAACCCGTATCACTACTCA
AAAACCCTTGCCGAGCGGGCCGCATGGGAGTTCATGCAGGAGCAGCCGGGTTTTGACCTGGTTGTCATCAACCCCTTCAT
GGTGATCGGTCCTTCTCTCGGAGCCTCGCTGAACACGACCAATCAGATGATACGCGACATTATAACCGGCGTCTATCCCG
GCATTCTTGATGTCAACTGGGGATTTGTCGATGTCCGTGATGTCGCCCTGGCTCATATCCGTGCCATAGAAAAACCCGCA
GCCCATGGTCGATACCTCTGTTCAGCAGAAGCGCTGGATATGCGTAACGTGGTCGCAATCCTCAGAAAGGCAGGGTATGG
AAACGACTATAAGTTGCCCTCTCTCGACCTGTCGGGAAAAGCAGCGACAACACTCATGAAACTCGTCTCATGGAGCCAGC
CACGCGATACCGGTGCCTACCTGAGAACACATCTCGGACGTCGTATGCGCTACGATAACGATAAAATCCGAAAGGAACTC
GACATCCAATTCCGAAATATCGAAAGCTCACTGCTTGAAACCGTCGAAGACCTTATCAGCTGGGGGCATCTGAAGAAGAA
GAGCTGA

Upstream 100 bases:

>100_bases
GTCAGCTTCCTCCAACACCAGGACCTGCCTGTTGTCATGAAGGCAATCAAAGCGCTGGCCGAATCCAGATATCATAAAAA
CAAGGAAAGCAACTCCTGAT

Downstream 100 bases:

>100_bases
AGAGCGCTTGAACGATCATCGAGCATGGTGCGGACCGGAACAAAGTCCGATCACTGACACGTCGTTGAGCCTGAACCTCT
GATCGCCTGCCTAACGTTCG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MAQHPIVCVTGATGFIAAHLIKKLLQQGYAVRGTVRKLSKHSSYRFLENLDGAAERLELIEADLLSPGSFDAAIGGTQYV
MHTASPYLINVRDPQKNLVEPALEGTRSVLESCRKASSVKRVVLTSSIAAITGAPDSNRVFTENDWNTTSSLKENPYHYS
KTLAERAAWEFMQEQPGFDLVVINPFMVIGPSLGASLNTTNQMIRDIITGVYPGILDVNWGFVDVRDVALAHIRAIEKPA
AHGRYLCSAEALDMRNVVAILRKAGYGNDYKLPSLDLSGKAATTLMKLVSWSQPRDTGAYLRTHLGRRMRYDNDKIRKEL
DIQFRNIESSLLETVEDLISWGHLKKKS

Sequences:

>Translated_348_residues
MAQHPIVCVTGATGFIAAHLIKKLLQQGYAVRGTVRKLSKHSSYRFLENLDGAAERLELIEADLLSPGSFDAAIGGTQYV
MHTASPYLINVRDPQKNLVEPALEGTRSVLESCRKASSVKRVVLTSSIAAITGAPDSNRVFTENDWNTTSSLKENPYHYS
KTLAERAAWEFMQEQPGFDLVVINPFMVIGPSLGASLNTTNQMIRDIITGVYPGILDVNWGFVDVRDVALAHIRAIEKPA
AHGRYLCSAEALDMRNVVAILRKAGYGNDYKLPSLDLSGKAATTLMKLVSWSQPRDTGAYLRTHLGRRMRYDNDKIRKEL
DIQFRNIESSLLETVEDLISWGHLKKKS
>Mature_347_residues
AQHPIVCVTGATGFIAAHLIKKLLQQGYAVRGTVRKLSKHSSYRFLENLDGAAERLELIEADLLSPGSFDAAIGGTQYVM
HTASPYLINVRDPQKNLVEPALEGTRSVLESCRKASSVKRVVLTSSIAAITGAPDSNRVFTENDWNTTSSLKENPYHYSK
TLAERAAWEFMQEQPGFDLVVINPFMVIGPSLGASLNTTNQMIRDIITGVYPGILDVNWGFVDVRDVALAHIRAIEKPAA
HGRYLCSAEALDMRNVVAILRKAGYGNDYKLPSLDLSGKAATTLMKLVSWSQPRDTGAYLRTHLGRRMRYDNDKIRKELD
IQFRNIESSLLETVEDLISWGHLKKKS

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987463, Length=346, Percent_Identity=34.393063583815, Blast_Score=193, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321281, Length=364, Percent_Identity=33.5164835164835, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6320750, Length=358, Percent_Identity=34.9162011173184, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6321399, Length=369, Percent_Identity=31.7073170731707, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6324421, Length=359, Percent_Identity=31.1977715877437, Blast_Score=142, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 38619; Mature: 38487

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQHPIVCVTGATGFIAAHLIKKLLQQGYAVRGTVRKLSKHSSYRFLENLDGAAERLELI
CCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EADLLSPGSFDAAIGGTQYVMHTASPYLINVRDPQKNLVEPALEGTRSVLESCRKASSVK
HHHHCCCCCCCHHCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVVLTSSIAAITGAPDSNRVFTENDWNTTSSLKENPYHYSKTLAERAAWEFMQEQPGFDL
HHHHHHHHHHEECCCCCCCEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCE
VVINPFMVIGPSLGASLNTTNQMIRDIITGVYPGILDVNWGFVDVRDVALAHIRAIEKPA
EEECCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCEEHHHHHHHHHHHHHHCCC
AHGRYLCSAEALDMRNVVAILRKAGYGNDYKLPSLDLSGKAATTLMKLVSWSQPRDTGAY
CCCCEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
LRTHLGRRMRYDNDKIRKELDIQFRNIESSLLETVEDLISWGHLKKKS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AQHPIVCVTGATGFIAAHLIKKLLQQGYAVRGTVRKLSKHSSYRFLENLDGAAERLELI
CCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EADLLSPGSFDAAIGGTQYVMHTASPYLINVRDPQKNLVEPALEGTRSVLESCRKASSVK
HHHHCCCCCCCHHCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RVVLTSSIAAITGAPDSNRVFTENDWNTTSSLKENPYHYSKTLAERAAWEFMQEQPGFDL
HHHHHHHHHHEECCCCCCCEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCE
VVINPFMVIGPSLGASLNTTNQMIRDIITGVYPGILDVNWGFVDVRDVALAHIRAIEKPA
EEECCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCEEHHHHHHHHHHHHHHCCC
AHGRYLCSAEALDMRNVVAILRKAGYGNDYKLPSLDLSGKAATTLMKLVSWSQPRDTGAY
CCCCEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHH
LRTHLGRRMRYDNDKIRKELDIQFRNIESSLLETVEDLISWGHLKKKS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]