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The map label for this gene is prfB [H]

Identifier: 194333053

GI number: 194333053

Start: 215896

End: 217041

Strand: Reverse

Name: prfB [H]

Synonym: Paes_0209

Alternate gene names: 194333053

Gene position: 217041-215896 (Counterclockwise)

Preceding gene: 194333058

Following gene: 194333052

Centisome position: 8.64

GC content: 52.09

Gene sequence:

>1146_bases
ATGCAAGAGGCTCGCAATACCACATTTATCACTCAAGAAGAAAGGATCTCATTATGTTTGAAGGAGAAAAAGAGAGGCTG
CACGAACTGCATCACCGTGTTGAACAGTTACGGGGGTATCTTTGACATCGATGAACGACTGAAAAAAATAGAAGAACTCG
AGCAGATCACAGCCGAACCCGACTTCTGGAACGATCAGAAGGAAGCCCACAGAATCGTCAAAGAAATCAATGAGCACAAG
ACCTGGACAGATGATTTCCAGAAAATTGCAGCCGACGTCGCCTCGCGCCAGGAAGAAGTCGAAATAGCTGAAGAGCTCGA
AGACGAATCACTGGGCTCAGAAATCGCCGGAGGAATTGATTCGATCGAAAAAGAGATCACCGCACTGGAATCGAAAAACA
TGCTCTCCGGCAAAGACGATCCAGGTAATGCGCTGCTGACCATCAATGCCGGTGCTGGCGGAACCGAAGCACAGGACTGG
GCGCAGATGCTCTACCGCATGTACATGCGCTGGGCTGAACGCAAAGGCTACAAGGTGTTCATGGACGACTACCAGGAAGG
CGAAGGAGCAGGCATTAAAACCGCAACACTCGAAATTGTCGGGCCCTATGCATACGGCTACCTGAAAGCCGAAAACGGCG
TCCACCGTCTGGTCAGAGTCTCGCCCTTCGACTCCAACGCCCGGCGTCATACATCGTTCGCCAGTGTCTACACCTATCCT
GAAGCCCCGCCCGATGCGGAAATTGACATCCGTAAGGAAGACCTTGAACTCTCCACCTTCCGAAGCGGCGGTAAAGGCGG
CCAGAACGTCAACAAGGTTGAAACCGCAGTGCGCATCAAGCACATCCCATCCGGCATCGTCGTCTCCTGCCAGCAGGAAC
GCTCTCAGTTCCAGAACCGTGAACGGGCAATAAAAATGCTGATGGCACAGCTCTACAAGAAACAGCGCGAAGAAGAGGAA
GCGCGAAAGCAGGCTGTGGAAGGTCAGAAAATGAAAATCGAATGGGGAAGCCAGATCCGCAGCTACGTCATGGACGACCG
GCGTATCAAGGACCACCGGACAAACCATGAGCGGCACGATATCGAAGCCGTGCTCGACGGAGATATCGACGACTTTATCG
AAAAGTATCTTTCGGAATTCTCCTGA

Upstream 100 bases:

>100_bases
GGCACGTTTACCCATCCCGGTACGACGTAACGTCATCCATCCTCTGCCACTCTTTGATTTCCATCAAAAAAATTGATTCT
TTAAAGAGATCTTAGAGCGC

Downstream 100 bases:

>100_bases
CCGCAACTATGACCGGCTCCTCCATCACGGTGCGTTTCGGCATCGTCGCCGACATTCACATGTCTGATTGCGCTGCAGAA
AAAGCCCGGACTGTCGAAGA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MQEARNTTFITQEERISLCLKEKKRGCTNCITVLNSYGGIFDIDERLKKIEELEQITAEPDFWNDQKEAHRIVKEINEHK
TWTDDFQKIAADVASRQEEVEIAEELEDESLGSEIAGGIDSIEKEITALESKNMLSGKDDPGNALLTINAGAGGTEAQDW
AQMLYRMYMRWAERKGYKVFMDDYQEGEGAGIKTATLEIVGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYTYP
EAPPDAEIDIRKEDLELSTFRSGGKGGQNVNKVETAVRIKHIPSGIVVSCQQERSQFQNRERAIKMLMAQLYKKQREEEE
ARKQAVEGQKMKIEWGSQIRSYVMDDRRIKDHRTNHERHDIEAVLDGDIDDFIEKYLSEFS

Sequences:

>Translated_381_residues
MQEARNTTFITQEERISLCLKEKKRGCTNCITVLNSYGGIFDIDERLKKIEELEQITAEPDFWNDQKEAHRIVKEINEHK
TWTDDFQKIAADVASRQEEVEIAEELEDESLGSEIAGGIDSIEKEITALESKNMLSGKDDPGNALLTINAGAGGTEAQDW
AQMLYRMYMRWAERKGYKVFMDDYQEGEGAGIKTATLEIVGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYTYP
EAPPDAEIDIRKEDLELSTFRSGGKGGQNVNKVETAVRIKHIPSGIVVSCQQERSQFQNRERAIKMLMAQLYKKQREEEE
ARKQAVEGQKMKIEWGSQIRSYVMDDRRIKDHRTNHERHDIEAVLDGDIDDFIEKYLSEFS
>Mature_381_residues
MQEARNTTFITQEERISLCLKEKKRGCTNCITVLNSYGGIFDIDERLKKIEELEQITAEPDFWNDQKEAHRIVKEINEHK
TWTDDFQKIAADVASRQEEVEIAEELEDESLGSEIAGGIDSIEKEITALESKNMLSGKDDPGNALLTINAGAGGTEAQDW
AQMLYRMYMRWAERKGYKVFMDDYQEGEGAGIKTATLEIVGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYTYP
EAPPDAEIDIRKEDLELSTFRSGGKGGQNVNKVETAVRIKHIPSGIVVSCQQERSQFQNRERAIKMLMAQLYKKQREEEE
ARKQAVEGQKMKIEWGSQIRSYVMDDRRIKDHRTNHERHDIEAVLDGDIDDFIEKYLSEFS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=286, Percent_Identity=36.013986013986, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI34577120, Length=252, Percent_Identity=35.3174603174603, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI166795305, Length=182, Percent_Identity=34.6153846153846, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=46.6076696165192, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1787462, Length=247, Percent_Identity=40.8906882591093, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17542784, Length=263, Percent_Identity=31.9391634980989, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=246, Percent_Identity=36.5853658536585, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI19921226, Length=263, Percent_Identity=37.6425855513308, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 43650; Mature: 43650

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEARNTTFITQEERISLCLKEKKRGCTNCITVLNSYGGIFDIDERLKKIEELEQITAEP
CCCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHCCC
DFWNDQKEAHRIVKEINEHKTWTDDFQKIAADVASRQEEVEIAEELEDESLGSEIAGGID
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SIEKEITALESKNMLSGKDDPGNALLTINAGAGGTEAQDWAQMLYRMYMRWAERKGYKVF
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
MDDYQEGEGAGIKTATLEIVGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYTYP
ECCCCCCCCCCCEEEEEEEECCHHHEEEECCCCCEEEEEECCCCCCCHHHHHHHHEEECC
EAPPDAEIDIRKEDLELSTFRSGGKGGQNVNKVETAVRIKHIPSGIVVSCQQERSQFQNR
CCCCCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHHHH
ERAIKMLMAQLYKKQREEEEARKQAVEGQKMKIEWGSQIRSYVMDDRRIKDHRTNHERHD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCHHHHHHCCCCHHHH
IEAVLDGDIDDFIEKYLSEFS
HHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQEARNTTFITQEERISLCLKEKKRGCTNCITVLNSYGGIFDIDERLKKIEELEQITAEP
CCCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHCCC
DFWNDQKEAHRIVKEINEHKTWTDDFQKIAADVASRQEEVEIAEELEDESLGSEIAGGID
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
SIEKEITALESKNMLSGKDDPGNALLTINAGAGGTEAQDWAQMLYRMYMRWAERKGYKVF
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
MDDYQEGEGAGIKTATLEIVGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYTYP
ECCCCCCCCCCCEEEEEEEECCHHHEEEECCCCCEEEEEECCCCCCCHHHHHHHHEEECC
EAPPDAEIDIRKEDLELSTFRSGGKGGQNVNKVETAVRIKHIPSGIVVSCQQERSQFQNR
CCCCCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHHHH
ERAIKMLMAQLYKKQREEEEARKQAVEGQKMKIEWGSQIRSYVMDDRRIKDHRTNHERHD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCHHHHHHCCCCHHHH
IEAVLDGDIDDFIEKYLSEFS
HHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]