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The map label for this gene is yhfE [H]

Identifier: 194332959

GI number: 194332959

Start: 114369

End: 115487

Strand: Reverse

Name: yhfE [H]

Synonym: Paes_0112

Alternate gene names: 194332959

Gene position: 115487-114369 (Counterclockwise)

Preceding gene: 194332960

Following gene: 194332956

Centisome position: 4.6

GC content: 56.03

Gene sequence:

>1119_bases
ATGATCACTATCGATACCGAATACCTCAAAACAACCCTCCTGAAACTGCTCGACATTCCAAGCCCGACCGGCTATACCGA
TGAAATCGTCCACGCTGTCGGCAGGGAGCTGGAACGCATGGGGGTCCCGTTCGAACTCACCCGCCGCGGAGCGATCCGTG
CAACGCTCAAGGGGGCGCAGCCAAAACTTGACAGGGCTATCGTCTCGCACCTCGACACACTCGGCGCCATGGTGAGAGAA
CTCAAACCCAATGGCAGGCTGGGAATCATGATGATAGGCCACTGGTCGGCCAGATTTGCTGAAGGCGCGAGAGTCACCAT
CTTTACCGATAAACGCTCCTTCCGCGGCACGATCCAGCCTCTCAAGGCATCCGGGCACGTCTACAACGACGAGATAGATA
CCCAGCCAGTCTCATGGGACAATCTTGAGGTACGGATCGATGAAAAACTCTCTACCCGGGAAGATCTCGAAGCGCTCGGC
TTCAATGTAGGCGACTTCATTGCCGTGGACAGCAGCCCGGAACTCACCTCCAGCGGATTCATCAAGGCACGCCATCTCGA
CAACAAAGCCGGTGTTGCCTGCCAGCTCACGACAATCAAGGCGATCGTTGACGCAGGCATAACACTTCCTGTCACCTGCC
ACCCGCTCTTTACCATCTCCGAAGAAGAAGGGACAGGCGCCTCGTCGATTCTGCACGGCGATGTCGCCGCCATGGTCGCT
ATAGACAATTCAACGGTCGCACCGGGGCAGAACTCCAGGGAAGACAGCGTTACCATCGCCATGCGCGATCAGGGCGCGAT
CTACGATTACCACCTCACCCATCGTCTTATCAGCCTCTGCGAAGACAACGCCATTCCCTATGTCCGCGATGTTTTCCGCC
ATTACAGGTCTGACGCTGCTTCTGCACTCTCTGCCGGAAACGATATCCGCACCGGGCTTATCTGCTTCGGGCTGGATGCT
TCGCACGGCCATGAAAGAACCCATATCGATTCACTCATAGCCATCTCCCGGCTTCTATGCGCCTACCTGCAAAGCACCCC
GAGAATCAAGCACGACAGCAACAAACTCGGGCCGCTGGAAGGCTTTCCAATGCTTTCAGTCAACAAAAAAACGCGTTAA

Upstream 100 bases:

>100_bases
AAGCCAATGAGCGGCCGGGCCTTGCCAATCATGAGCCCCAGCCGACCGCCGAACGGTTTATCGATTTTCTTTTTCCTCAA
AGCATTGCAAGGACTCTTCC

Downstream 100 bases:

>100_bases
CCGCCGCATCCTGTTCAACCGGGCATATGACCTTTATCAGACATATGTCCGGCTGCATCCCAGCCGAGCTCCTTGTCCTT
GTGCTCTTCGCGTTTCTCCG

Product: hydrolase, peptidase M42 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MITIDTEYLKTTLLKLLDIPSPTGYTDEIVHAVGRELERMGVPFELTRRGAIRATLKGAQPKLDRAIVSHLDTLGAMVRE
LKPNGRLGIMMIGHWSARFAEGARVTIFTDKRSFRGTIQPLKASGHVYNDEIDTQPVSWDNLEVRIDEKLSTREDLEALG
FNVGDFIAVDSSPELTSSGFIKARHLDNKAGVACQLTTIKAIVDAGITLPVTCHPLFTISEEEGTGASSILHGDVAAMVA
IDNSTVAPGQNSREDSVTIAMRDQGAIYDYHLTHRLISLCEDNAIPYVRDVFRHYRSDAASALSAGNDIRTGLICFGLDA
SHGHERTHIDSLIAISRLLCAYLQSTPRIKHDSNKLGPLEGFPMLSVNKKTR

Sequences:

>Translated_372_residues
MITIDTEYLKTTLLKLLDIPSPTGYTDEIVHAVGRELERMGVPFELTRRGAIRATLKGAQPKLDRAIVSHLDTLGAMVRE
LKPNGRLGIMMIGHWSARFAEGARVTIFTDKRSFRGTIQPLKASGHVYNDEIDTQPVSWDNLEVRIDEKLSTREDLEALG
FNVGDFIAVDSSPELTSSGFIKARHLDNKAGVACQLTTIKAIVDAGITLPVTCHPLFTISEEEGTGASSILHGDVAAMVA
IDNSTVAPGQNSREDSVTIAMRDQGAIYDYHLTHRLISLCEDNAIPYVRDVFRHYRSDAASALSAGNDIRTGLICFGLDA
SHGHERTHIDSLIAISRLLCAYLQSTPRIKHDSNKLGPLEGFPMLSVNKKTR
>Mature_372_residues
MITIDTEYLKTTLLKLLDIPSPTGYTDEIVHAVGRELERMGVPFELTRRGAIRATLKGAQPKLDRAIVSHLDTLGAMVRE
LKPNGRLGIMMIGHWSARFAEGARVTIFTDKRSFRGTIQPLKASGHVYNDEIDTQPVSWDNLEVRIDEKLSTREDLEALG
FNVGDFIAVDSSPELTSSGFIKARHLDNKAGVACQLTTIKAIVDAGITLPVTCHPLFTISEEEGTGASSILHGDVAAMVA
IDNSTVAPGQNSREDSVTIAMRDQGAIYDYHLTHRLISLCEDNAIPYVRDVFRHYRSDAASALSAGNDIRTGLICFGLDA
SHGHERTHIDSLIAISRLLCAYLQSTPRIKHDSNKLGPLEGFPMLSVNKKTR

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=168, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: NA

Molecular weight: Translated: 40800; Mature: 40800

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIDTEYLKTTLLKLLDIPSPTGYTDEIVHAVGRELERMGVPFELTRRGAIRATLKGAQ
CEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCC
PKLDRAIVSHLDTLGAMVRELKPNGRLGIMMIGHWSARFAEGARVTIFTDKRSFRGTIQP
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCCCEEEEEECCCCCCCCCCE
LKASGHVYNDEIDTQPVSWDNLEVRIDEKLSTREDLEALGFNVGDFIAVDSSPELTSSGF
ECCCCCEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCC
IKARHLDNKAGVACQLTTIKAIVDAGITLPVTCHPLFTISEEEGTGASSILHGDVAAMVA
EEEEECCCCCCCEEEEHHHHHHHHCCCEEEEEECEEEEEECCCCCCHHHHHHCCEEEEEE
IDNSTVAPGQNSREDSVTIAMRDQGAIYDYHLTHRLISLCEDNAIPYVRDVFRHYRSDAA
ECCCEECCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SALSAGNDIRTGLICFGLDASHGHERTHIDSLIAISRLLCAYLQSTPRIKHDSNKLGPLE
HHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GFPMLSVNKKTR
CCCEEECCCCCC
>Mature Secondary Structure
MITIDTEYLKTTLLKLLDIPSPTGYTDEIVHAVGRELERMGVPFELTRRGAIRATLKGAQ
CEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCC
PKLDRAIVSHLDTLGAMVRELKPNGRLGIMMIGHWSARFAEGARVTIFTDKRSFRGTIQP
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCCCEEEEEECCCCCCCCCCE
LKASGHVYNDEIDTQPVSWDNLEVRIDEKLSTREDLEALGFNVGDFIAVDSSPELTSSGF
ECCCCCEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCC
IKARHLDNKAGVACQLTTIKAIVDAGITLPVTCHPLFTISEEEGTGASSILHGDVAAMVA
EEEEECCCCCCCEEEEHHHHHHHHCCCEEEEEECEEEEEECCCCCCHHHHHHCCEEEEEE
IDNSTVAPGQNSREDSVTIAMRDQGAIYDYHLTHRLISLCEDNAIPYVRDVFRHYRSDAA
ECCCEECCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SALSAGNDIRTGLICFGLDASHGHERTHIDSLIAISRLLCAYLQSTPRIKHDSNKLGPLE
HHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GFPMLSVNKKTR
CCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]