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The map label for this gene is amy [H]

Identifier: 194332951

GI number: 194332951

Start: 103392

End: 105353

Strand: Reverse

Name: amy [H]

Synonym: Paes_0104

Alternate gene names: 194332951

Gene position: 105353-103392 (Counterclockwise)

Preceding gene: 194332954

Following gene: 194332949

Centisome position: 4.19

GC content: 57.59

Gene sequence:

>1962_bases
ATGACAGAGCAGAGCAATGAGCACCATACCATCGAAAGACGCCTCGATCAGATAGATCTCAACGAGCTTTGCCGCGGCAG
AAGCTTTCACCCGTCGCCCGTATCGTGGGGTGACGAAGTCCTCTATTTCCTGTTTCTCGACCGGTTCTCGGACGCAAAAG
AGTACGGAGGATTCACCGACCGGGAAGGCAGGCCTGTCGATAGCGGTGAAAACGGACGTTCGACGCCGCTCTTCAACTTC
GAAGATGATGCTGCTCGCGCATCGAGGAGTGAATGGTTCGAATCAGGAAAAGGATGGTGCGGCGGCACTCTTGCGGGTCT
GCGTGACAAACTTGGCTACCTGAAACGGCTTGGCATCACAGCACTGTGGATCAGTCCCGTCTTCAAACAGGTCACCGGCA
GCAACGACTATCACGGCTACGGCATCCAGAACTTTCTTGACGTTGATCCGCATTTCGGAACCCGGCAAGAGCTCAGAGAA
CTCGTGCAGGCAGCGCACGCTGAAGGCATACGGGTCATTCTCGATATCATCGTCAACCACGCTGGAGACGTCTTCGCCTA
TAGCGGCAACGAAAGGCGCAACTACAATAACGGAGTGGAATTTCCCGTGCAGGGGTTCCGTCGATTCAGCGGCGAAGAAG
GAAGCATTCCATTCAGGACCGTGAGCCCTGAAGAAGAGCAGGAGCTCTGGCCTGACGGAGCCGTCTGGCCTGCGGAGCTG
CAAGAGCCCGGCAACTGGCGGAAAAAGGGCGAAATCGGCAACTGGGACGGGTTTCCCGACTATGTCGAGGGTGATTTCCT
TTCGCTCAAAGACCTGCATCTCGGCAATGGGATCAGCGACCCCTCGGCCGGTCAGGATATCGGCAGAAGAATCAGCGGAT
TCTCTCCGTCCGAAACCCTGGCGCACCTTATCAAGGTCTACCGCTTCTGGATTGCCTATGCAGACATCGACGGCTACCGT
CTCGACACCGTCAAACACATGGAACCCGGAGCGGTCCGTCTCTTTGTCAACGCCATTCATGAATTCGCCCAGTCGGTCGG
CAAGGAGAACTTCACCGTTATCGGAGAAATCACGGGAGGACGCGCTCTTGCCTTCGAAACGCTTGAAACCACCGGCCTTG
ATGCAGCACTCGGCATCAACGACGTTTCCGACAAGCTCGAATTTCTCGCTAAAGGGTGGCGCAGCCCCGGCCACCCCGAA
ACACCTGAACAGGAAGGCTACTTCGATATCTTCCGCAACAGTCTCCAGGACTGCAAGAGCAGCCATCAGTGGTATGGCAA
TCATATCGTCACCATGTTCGACGATCACGACCAGGTCGGCGTACGGCACAAATTCCGTTTCTGCGGACAGGGAGAACAGA
GCTACACCCTGCTGCCGGCAGCACTCGGGCTCAATCTTGCGACGATGGGTATCCCCTGCCTCTACTACGGAACCGAACAG
GCCTTCAACGGCGCCGACCACCGCGACAACGACGACTCGTACAGTGATGTCTTCCTGCGGGAGTGCATGTTCGGAGGAGC
GTTCGGATCGATGCAGAGCACCGGACGCCACTTCTTCAACGAATCGCATGAGATCTACCGCTTCATCAGTCGCCTTTCCG
CCCTGAGATCGCAACACCTTGCGCTGAGACGGGGACGTCAGTACCTCCGGCAGGTATCAGCCTCGGGTCATGACAACGAC
TTTCACTACCCGCAGCCCCTTGGCGGCGAACTGCGCTGGATCATCGCATGGTCCCGCATCTTCGCAGACCGGGAGTACCT
CTGTGCCTGCAATACCGACCCGCGCCACCCCCTGACGCTCTGGGCCACAGTGGACAGTTCGCTCCACCGCTCCGGCGAGA
CCATGAGCTGCCTGTTCAGCTCGGACCGGGAGCAGGAAGGAACATCCTCTGACATTGAAGCACGCAACGGCAAGGCGATT
GCCGTAACAGTGCCTCCGGGAGGGTTCGTGGTCTATCATTGA

Upstream 100 bases:

>100_bases
ACTTTTCTCACGCTGAGTCAGATATTCACGCAAACGTCACAGGACCAACACAAAAAATGCGTATTTTTCGTCCATTTATC
AACCCGACGCCCCAATCATT

Downstream 100 bases:

>100_bases
AACGGTAGAGAACGGGTCGGTGAACAAGAGGTTACCGACCCTGAACTGAAGGGGAAGCCGACAAGTCTACTTTCTTCGGC
CAGCCGCCTGGCGGGCTGCA

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MTEQSNEHHTIERRLDQIDLNELCRGRSFHPSPVSWGDEVLYFLFLDRFSDAKEYGGFTDREGRPVDSGENGRSTPLFNF
EDDAARASRSEWFESGKGWCGGTLAGLRDKLGYLKRLGITALWISPVFKQVTGSNDYHGYGIQNFLDVDPHFGTRQELRE
LVQAAHAEGIRVILDIIVNHAGDVFAYSGNERRNYNNGVEFPVQGFRRFSGEEGSIPFRTVSPEEEQELWPDGAVWPAEL
QEPGNWRKKGEIGNWDGFPDYVEGDFLSLKDLHLGNGISDPSAGQDIGRRISGFSPSETLAHLIKVYRFWIAYADIDGYR
LDTVKHMEPGAVRLFVNAIHEFAQSVGKENFTVIGEITGGRALAFETLETTGLDAALGINDVSDKLEFLAKGWRSPGHPE
TPEQEGYFDIFRNSLQDCKSSHQWYGNHIVTMFDDHDQVGVRHKFRFCGQGEQSYTLLPAALGLNLATMGIPCLYYGTEQ
AFNGADHRDNDDSYSDVFLRECMFGGAFGSMQSTGRHFFNESHEIYRFISRLSALRSQHLALRRGRQYLRQVSASGHDND
FHYPQPLGGELRWIIAWSRIFADREYLCACNTDPRHPLTLWATVDSSLHRSGETMSCLFSSDREQEGTSSDIEARNGKAI
AVTVPPGGFVVYH

Sequences:

>Translated_653_residues
MTEQSNEHHTIERRLDQIDLNELCRGRSFHPSPVSWGDEVLYFLFLDRFSDAKEYGGFTDREGRPVDSGENGRSTPLFNF
EDDAARASRSEWFESGKGWCGGTLAGLRDKLGYLKRLGITALWISPVFKQVTGSNDYHGYGIQNFLDVDPHFGTRQELRE
LVQAAHAEGIRVILDIIVNHAGDVFAYSGNERRNYNNGVEFPVQGFRRFSGEEGSIPFRTVSPEEEQELWPDGAVWPAEL
QEPGNWRKKGEIGNWDGFPDYVEGDFLSLKDLHLGNGISDPSAGQDIGRRISGFSPSETLAHLIKVYRFWIAYADIDGYR
LDTVKHMEPGAVRLFVNAIHEFAQSVGKENFTVIGEITGGRALAFETLETTGLDAALGINDVSDKLEFLAKGWRSPGHPE
TPEQEGYFDIFRNSLQDCKSSHQWYGNHIVTMFDDHDQVGVRHKFRFCGQGEQSYTLLPAALGLNLATMGIPCLYYGTEQ
AFNGADHRDNDDSYSDVFLRECMFGGAFGSMQSTGRHFFNESHEIYRFISRLSALRSQHLALRRGRQYLRQVSASGHDND
FHYPQPLGGELRWIIAWSRIFADREYLCACNTDPRHPLTLWATVDSSLHRSGETMSCLFSSDREQEGTSSDIEARNGKAI
AVTVPPGGFVVYH
>Mature_652_residues
TEQSNEHHTIERRLDQIDLNELCRGRSFHPSPVSWGDEVLYFLFLDRFSDAKEYGGFTDREGRPVDSGENGRSTPLFNFE
DDAARASRSEWFESGKGWCGGTLAGLRDKLGYLKRLGITALWISPVFKQVTGSNDYHGYGIQNFLDVDPHFGTRQELREL
VQAAHAEGIRVILDIIVNHAGDVFAYSGNERRNYNNGVEFPVQGFRRFSGEEGSIPFRTVSPEEEQELWPDGAVWPAELQ
EPGNWRKKGEIGNWDGFPDYVEGDFLSLKDLHLGNGISDPSAGQDIGRRISGFSPSETLAHLIKVYRFWIAYADIDGYRL
DTVKHMEPGAVRLFVNAIHEFAQSVGKENFTVIGEITGGRALAFETLETTGLDAALGINDVSDKLEFLAKGWRSPGHPET
PEQEGYFDIFRNSLQDCKSSHQWYGNHIVTMFDDHDQVGVRHKFRFCGQGEQSYTLLPAALGLNLATMGIPCLYYGTEQA
FNGADHRDNDDSYSDVFLRECMFGGAFGSMQSTGRHFFNESHEIYRFISRLSALRSQHLALRRGRQYLRQVSASGHDNDF
HYPQPLGGELRWIIAWSRIFADREYLCACNTDPRHPLTLWATVDSSLHRSGETMSCLFSSDREQEGTSSDIEARNGKAIA
VTVPPGGFVVYH

Specific function: Since Only Maltooligosaccharides Up To A Chain Length Of 6 Glucose Units Are Actively Transported Through The Cytoplasmic Membrane VIa The Membrane-Bound Complex Of Three Proteins, Malf, Malg, And Malk, Longer Maltooligosaccharides Must First Be Degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=83, Percent_Identity=39.7590361445783, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1789995, Length=345, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790687, Length=412, Percent_Identity=24.7572815533981, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1786604, Length=112, Percent_Identity=35.7142857142857, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322245, Length=86, Percent_Identity=37.2093023255814, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322241, Length=85, Percent_Identity=36.4705882352941, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322021, Length=85, Percent_Identity=36.4705882352941, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324416, Length=85, Percent_Identity=36.4705882352941, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321726, Length=85, Percent_Identity=36.4705882352941, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321731, Length=100, Percent_Identity=32, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319776, Length=85, Percent_Identity=35.2941176470588, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24586587, Length=91, Percent_Identity=42.8571428571429, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24586597, Length=99, Percent_Identity=38.3838383838384, Blast_Score=74, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24586591, Length=91, Percent_Identity=39.5604395604396, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24586599, Length=91, Percent_Identity=37.3626373626374, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI221330053, Length=82, Percent_Identity=39.0243902439024, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24586593, Length=91, Percent_Identity=39.5604395604396, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583749, Length=91, Percent_Identity=39.5604395604396, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583747, Length=91, Percent_Identity=39.5604395604396, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45549022, Length=106, Percent_Identity=33.9622641509434, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586589, Length=91, Percent_Identity=36.2637362637363, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005323 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF03714 PUD [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 73595; Mature: 73464

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQSNEHHTIERRLDQIDLNELCRGRSFHPSPVSWGDEVLYFLFLDRFSDAKEYGGFTD
CCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
REGRPVDSGENGRSTPLFNFEDDAARASRSEWFESGKGWCGGTLAGLRDKLGYLKRLGIT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHH
ALWISPVFKQVTGSNDYHGYGIQNFLDVDPHFGTRQELRELVQAAHAEGIRVILDIIVNH
HHHHHHHHHHHCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
AGDVFAYSGNERRNYNNGVEFPVQGFRRFSGEEGSIPFRTVSPEEEQELWPDGAVWPAEL
CCCEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCHHHHCCCCCCCCCHHH
QEPGNWRKKGEIGNWDGFPDYVEGDFLSLKDLHLGNGISDPSAGQDIGRRISGFSPSETL
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHEECCCCCCCCCCCHHHHHHHCCCCHHHHH
AHLIKVYRFWIAYADIDGYRLDTVKHMEPGAVRLFVNAIHEFAQSVGKENFTVIGEITGG
HHHHHHHHHHHHHCCCCCEEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
RALAFETLETTGLDAALGINDVSDKLEFLAKGWRSPGHPETPEQEGYFDIFRNSLQDCKS
CEEEEEHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SHQWYGNHIVTMFDDHDQVGVRHKFRFCGQGEQSYTLLPAALGLNLATMGIPCLYYGTEQ
HHHCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCEEEEHHHHCCCHHHHCCCEEEECCHH
AFNGADHRDNDDSYSDVFLRECMFGGAFGSMQSTGRHFFNESHEIYRFISRLSALRSQHL
HCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ALRRGRQYLRQVSASGHDNDFHYPQPLGGELRWIIAWSRIFADREYLCACNTDPRHPLTL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCEEEECCCCCCCCEEE
WATVDSSLHRSGETMSCLFSSDREQEGTSSDIEARNGKAIAVTVPPGGFVVYH
EEECCHHHHHCCCCEEEHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEC
>Mature Secondary Structure 
TEQSNEHHTIERRLDQIDLNELCRGRSFHPSPVSWGDEVLYFLFLDRFSDAKEYGGFTD
CCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
REGRPVDSGENGRSTPLFNFEDDAARASRSEWFESGKGWCGGTLAGLRDKLGYLKRLGIT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHH
ALWISPVFKQVTGSNDYHGYGIQNFLDVDPHFGTRQELRELVQAAHAEGIRVILDIIVNH
HHHHHHHHHHHCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
AGDVFAYSGNERRNYNNGVEFPVQGFRRFSGEEGSIPFRTVSPEEEQELWPDGAVWPAEL
CCCEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCHHHHCCCCCCCCCHHH
QEPGNWRKKGEIGNWDGFPDYVEGDFLSLKDLHLGNGISDPSAGQDIGRRISGFSPSETL
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHEECCCCCCCCCCCHHHHHHHCCCCHHHHH
AHLIKVYRFWIAYADIDGYRLDTVKHMEPGAVRLFVNAIHEFAQSVGKENFTVIGEITGG
HHHHHHHHHHHHHCCCCCEEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
RALAFETLETTGLDAALGINDVSDKLEFLAKGWRSPGHPETPEQEGYFDIFRNSLQDCKS
CEEEEEHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SHQWYGNHIVTMFDDHDQVGVRHKFRFCGQGEQSYTLLPAALGLNLATMGIPCLYYGTEQ
HHHCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCEEEEHHHHCCCHHHHCCCEEEECCHH
AFNGADHRDNDDSYSDVFLRECMFGGAFGSMQSTGRHFFNESHEIYRFISRLSALRSQHL
HCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ALRRGRQYLRQVSASGHDNDFHYPQPLGGELRWIIAWSRIFADREYLCACNTDPRHPLTL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCEEEECCCCCCCCEEE
WATVDSSLHRSGETMSCLFSSDREQEGTSSDIEARNGKAIAVTVPPGGFVVYH
EEECCHHHHHCCCCEEEHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8424949; 8485150 [H]