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The map label for this gene is nifB [H]

Identifier: 193213269

GI number: 193213269

Start: 1777887

End: 1779161

Strand: Direct

Name: nifB [H]

Synonym: Cpar_1626

Alternate gene names: 193213269

Gene position: 1777887-1779161 (Clockwise)

Preceding gene: 193213268

Following gene: 193213270

Centisome position: 77.66

GC content: 61.18

Gene sequence:

>1275_bases
ATGACACTCAACATCCAGAACCACCCGTGCTTCAACGACTCCGCGAGGCACACCTTCGGAAGAATCCACCTGCCGGTCGC
GCCGAAATGCAACATCCAGTGCAACTACTGCAACCGGAAGTTCGACTGCATGAACGAGAACCGCCCCGGCGTGTCGAGCA
AGGTGCTCTCGCCGAAGCAGGCGCTCTACTACCTCGACAACGCCATCAAGCTCTCGCCGAACATCTCGGTGGTGGGCATC
GCAGGCCCCGGTGACCCGTTCGCTAACCCGGAGGAGACGATGGAGACCCTGCGGCTGGTGCGCGAAAAGTACCCCGACAT
GCTGCTCTGCGTCGCCACCAACGGCCTCGACGTGCTGCCCTACATCGAAGAACTTGCCGAGCTGAAGGTGAGCCACGTAA
CGCTCACGATCAACGCCATCGATCCGGAGATCGGCCAGGAGATCTACGCCTGGGTACGCTACCAGAAGCGCATGTACCGC
GACCGTCAGGCTGCCGAACTGCTGCTCGAAAACCAGCTTGCCGCCCTGCAGAAGCTCAAGAGGTTCGGTGTCACGGCCAA
GGTGAACTCGATCATCATCCCCGGCATCAACGACAAGCACGTGGTCGAAGTGGCCCGCCAGGTCGCCTCGATGGGCGCGG
ACATCCTGAACGCCCTGCCCTACTACAACACCACTGAGACGGTCTTCGAGAACATTCCCGAACCCGATCCGATGATGGTC
AGGGAGATTCAGGACGAAGCTGGCAAACTGCTGCCGCAGATGAAGCACTGCGCCCGCTGCCGAGCTGACGCGGCTGGAAT
CATCGGCGAAATCAACAGCGACGAGATGATGCAGAAGCTCGCCGAAGCCTCGCTCATGCCGAAAAATCCCGACGAGCACC
GCCCCTACATCGCCGTTGCCAGCCTTGAAGGCGTGCTCATCAACCAGCACCTCGGCGAAGCTGACAGGTTTTTGATCTAC
GAGCTTAACGAGGACAAAAAAGGCTGCACACTGGTCGATTCGCGCCCGGCCCCGCCCGCCGGTGGCGGCAAGGCTCGCTG
GGAAGCGCTCGCCGCGAAGCTCTCCGACTGCCGCGCCGTGCTGGTCAACAGCGCAGGCGACGCCCCGCAAATGGTGCTCA
AAGCCAGTGGCGTCGATGTGATGTCGCTCGAAGGGGTGATCGAAGAGGCGGTGTACGGCGTCTTCACCGGTCAGGATCTC
AAGCACCTGATGAAAAGCAGCCAGATCCACGCCTGCAAAACAAGCTGCAGCGGCGACGGCAACGGGTGCGATTAA

Upstream 100 bases:

>100_bases
AGGCTACACGTACATCTAAAGGAAAACGCATGAAGTAATCACCCGGCATCTCGATCCAGAAAACGGAGACGCCGGAAAAG
TCTGAAGGGAGCAATAGATC

Downstream 100 bases:

>100_bases
CAATAAAAAGATCAGTCGGATCAGTCAGATCTGAGCGATTCGACTGATCTGGCCCCCGTTTTAAAAACCGGGGCAACTGA
AAAAAATGTAAGAGCAAATA

Product: nitrogenase cofactor biosynthesis protein NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTLNIQNHPCFNDSARHTFGRIHLPVAPKCNIQCNYCNRKFDCMNENRPGVSSKVLSPKQALYYLDNAIKLSPNISVVGI
AGPGDPFANPEETMETLRLVREKYPDMLLCVATNGLDVLPYIEELAELKVSHVTLTINAIDPEIGQEIYAWVRYQKRMYR
DRQAAELLLENQLAALQKLKRFGVTAKVNSIIIPGINDKHVVEVARQVASMGADILNALPYYNTTETVFENIPEPDPMMV
REIQDEAGKLLPQMKHCARCRADAAGIIGEINSDEMMQKLAEASLMPKNPDEHRPYIAVASLEGVLINQHLGEADRFLIY
ELNEDKKGCTLVDSRPAPPAGGGKARWEALAAKLSDCRAVLVNSAGDAPQMVLKASGVDVMSLEGVIEEAVYGVFTGQDL
KHLMKSSQIHACKTSCSGDGNGCD

Sequences:

>Translated_424_residues
MTLNIQNHPCFNDSARHTFGRIHLPVAPKCNIQCNYCNRKFDCMNENRPGVSSKVLSPKQALYYLDNAIKLSPNISVVGI
AGPGDPFANPEETMETLRLVREKYPDMLLCVATNGLDVLPYIEELAELKVSHVTLTINAIDPEIGQEIYAWVRYQKRMYR
DRQAAELLLENQLAALQKLKRFGVTAKVNSIIIPGINDKHVVEVARQVASMGADILNALPYYNTTETVFENIPEPDPMMV
REIQDEAGKLLPQMKHCARCRADAAGIIGEINSDEMMQKLAEASLMPKNPDEHRPYIAVASLEGVLINQHLGEADRFLIY
ELNEDKKGCTLVDSRPAPPAGGGKARWEALAAKLSDCRAVLVNSAGDAPQMVLKASGVDVMSLEGVIEEAVYGVFTGQDL
KHLMKSSQIHACKTSCSGDGNGCD
>Mature_423_residues
TLNIQNHPCFNDSARHTFGRIHLPVAPKCNIQCNYCNRKFDCMNENRPGVSSKVLSPKQALYYLDNAIKLSPNISVVGIA
GPGDPFANPEETMETLRLVREKYPDMLLCVATNGLDVLPYIEELAELKVSHVTLTINAIDPEIGQEIYAWVRYQKRMYRD
RQAAELLLENQLAALQKLKRFGVTAKVNSIIIPGINDKHVVEVARQVASMGADILNALPYYNTTETVFENIPEPDPMMVR
EIQDEAGKLLPQMKHCARCRADAAGIIGEINSDEMMQKLAEASLMPKNPDEHRPYIAVASLEGVLINQHLGEADRFLIYE
LNEDKKGCTLVDSRPAPPAGGGKARWEALAAKLSDCRAVLVNSAGDAPQMVLKASGVDVMSLEGVIEEAVYGVFTGQDLK
HLMKSSQIHACKTSCSGDGNGCD

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 46677; Mature: 46546

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNIQNHPCFNDSARHTFGRIHLPVAPKCNIQCNYCNRKFDCMNENRPGVSSKVLSPKQ
CEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCHHHCCHHH
ALYYLDNAIKLSPNISVVGIAGPGDPFANPEETMETLRLVREKYPDMLLCVATNGLDVLP
HHHHHCCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
YIEELAELKVSHVTLTINAIDPEIGQEIYAWVRYQKRMYRDRQAAELLLENQLAALQKLK
HHHHHHHCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFGVTAKVNSIIIPGINDKHVVEVARQVASMGADILNALPYYNTTETVFENIPEPDPMMV
HCCCEEEECEEEECCCCCHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHCCCCCCHHHH
REIQDEAGKLLPQMKHCARCRADAAGIIGEINSDEMMQKLAEASLMPKNPDEHRPYIAVA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEE
SLEGVLINQHLGEADRFLIYELNEDKKGCTLVDSRPAPPAGGGKARWEALAAKLSDCRAV
ECCCEEEHHHCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHEE
LVNSAGDAPQMVLKASGVDVMSLEGVIEEAVYGVFTGQDLKHLMKSSQIHACKTSCSGDG
EECCCCCCHHHHHEECCCCEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCCCC
NGCD
CCCC
>Mature Secondary Structure 
TLNIQNHPCFNDSARHTFGRIHLPVAPKCNIQCNYCNRKFDCMNENRPGVSSKVLSPKQ
EECCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCHHHCCHHH
ALYYLDNAIKLSPNISVVGIAGPGDPFANPEETMETLRLVREKYPDMLLCVATNGLDVLP
HHHHHCCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHH
YIEELAELKVSHVTLTINAIDPEIGQEIYAWVRYQKRMYRDRQAAELLLENQLAALQKLK
HHHHHHHCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFGVTAKVNSIIIPGINDKHVVEVARQVASMGADILNALPYYNTTETVFENIPEPDPMMV
HCCCEEEECEEEECCCCCHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHCCCCCCHHHH
REIQDEAGKLLPQMKHCARCRADAAGIIGEINSDEMMQKLAEASLMPKNPDEHRPYIAVA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEE
SLEGVLINQHLGEADRFLIYELNEDKKGCTLVDSRPAPPAGGGKARWEALAAKLSDCRAV
ECCCEEEHHHCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHEE
LVNSAGDAPQMVLKASGVDVMSLEGVIEEAVYGVFTGQDLKHLMKSSQIHACKTSCSGDG
EECCCCCCHHHHHEECCCCEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCCCC
NGCD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]