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The map label for this gene is nifK2 [H]

Identifier: 193213268

GI number: 193213268

Start: 1776438

End: 1777805

Strand: Direct

Name: nifK2 [H]

Synonym: Cpar_1625

Alternate gene names: 193213268

Gene position: 1776438-1777805 (Clockwise)

Preceding gene: 193213267

Following gene: 193213269

Centisome position: 77.6

GC content: 61.04

Gene sequence:

>1368_bases
ATGACCACGAACGCTTCGACCATGACGGCCAAAACGGCCACGCAGAACGCATGCAAGCTCTGCACTCCGCTCGGCGCGTG
CCTGGCCTTCCGCGGCATCGAAAAGTGCGTACCGTTCCTGCACGGCTCGCAGGGGTGTGCCACCTACATCCGCCGCTACC
TCATCAGCCACTACAAGGAGCCGATCGACATCGCCTCATCCAACTTCAACGAAGAGACGGCGGTCTTCGGCGGCAGCCAC
AACCTGAAGCTGGGGCTGAAAAACGTCACCCAGCAGTACAAGCCGCAGGTGATCGGCATCGCGACGACCTGCCTGTCGGA
GACCATCGGCGACGACGTGCCGATGATTCTGAAGGACTACAAGGCGATCATGGGTGAGGCCGATCTGCCGACCATGATCT
TCGCCTCGACGCCGAGCTACACCGGCAGCCACATCGACGGCTTCCACACCGCCGTACGCTCCGCCGTGCAGACCTTCGCC
ACCGGCGGCGCGAAGAAGAACATGCTCAACCTCTTTTCGGGAATGATTTCGCCGGCGGACATCCGCTACCTGAAGGAGAT
TCTCAAGGAGTTCGGAATGCCGTTCATGCTGCTGCCGGACTACTCGCAGACGCTCGACGGCGGCCCGTGGGGCGAATACC
ACCGCATTCCGCCCGGCGGCACGCCGACCAGCGCCATCGCCGACAGCGGCAGCGCGGCAGCCAGCATCGAGTTCGGCTCG
ACGCTCGAAGCTAAAAAATCGGCGGCAGGCTACCTCGAAGAGACCTTCGGAGTTCCACGCCACAGCATGCCCCTGCCGAT
CGGCATCAAGGCAACCGACCGCTTCTTCGCGCTGCTCGAAGAGCTGACCGAGAAACCGATGCCTGAAAAGTATGAAGATG
AGCGCCGCCGCCTGGTTGACGCCTACGCCGACGGACACAAGTACGTCTTCGGCAGAAAGGCGATCCTCTACGGCGAGGAG
GATCTGGTGATTTCGATGGCCGCCTTCCTGCGCGAAATCGGCGTCGTGCCAGTGCTCTGCGCCTCGGGCGGCAAGAGCGG
GCTGATGAAAAAGCGGATGCTGGAGCTGATTCCCGATCTTGAAGAGCTTGAAATCCAGGTGCGCGAAGGCGTTGACTTCG
TGGACATCGAAAACGAGGCCAAGGTGCTCAAGCCGGACTTTCTCATCGGCAACAGCAAAGGGTTCACGATGTCGCGCAAG
AACGAGCTTCAGCTCATCAGGATCGGCTTCCCGATTCACGATCGCTTCGGCGGCCAGCGCCTACACCACCTCGGCTACAA
AGGCACGCAGGAGCTGTTCGACCGCATCGTCAACACCGTCATCGAGTATCGCCAGAACTCGTCACCGGTAGGCTACACGT
ACATCTAA

Upstream 100 bases:

>100_bases
CGCTCGCCGGATTCATCGGCATGTACTATTTCGCCAAGGAGGTTTACGAATCGGTGATGAGCCCCGTGTGGCAGTTCGCC
CCGAGAAAAGGAGGTGTCAA

Downstream 100 bases:

>100_bases
AGGAAAACGCATGAAGTAATCACCCGGCATCTCGATCCAGAAAACGGAGACGCCGGAAAAGTCTGAAGGGAGCAATAGAT
CATGACACTCAACATCCAGA

Product: nitrogenase

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTTNASTMTAKTATQNACKLCTPLGACLAFRGIEKCVPFLHGSQGCATYIRRYLISHYKEPIDIASSNFNEETAVFGGSH
NLKLGLKNVTQQYKPQVIGIATTCLSETIGDDVPMILKDYKAIMGEADLPTMIFASTPSYTGSHIDGFHTAVRSAVQTFA
TGGAKKNMLNLFSGMISPADIRYLKEILKEFGMPFMLLPDYSQTLDGGPWGEYHRIPPGGTPTSAIADSGSAAASIEFGS
TLEAKKSAAGYLEETFGVPRHSMPLPIGIKATDRFFALLEELTEKPMPEKYEDERRRLVDAYADGHKYVFGRKAILYGEE
DLVISMAAFLREIGVVPVLCASGGKSGLMKKRMLELIPDLEELEIQVREGVDFVDIENEAKVLKPDFLIGNSKGFTMSRK
NELQLIRIGFPIHDRFGGQRLHHLGYKGTQELFDRIVNTVIEYRQNSSPVGYTYI

Sequences:

>Translated_455_residues
MTTNASTMTAKTATQNACKLCTPLGACLAFRGIEKCVPFLHGSQGCATYIRRYLISHYKEPIDIASSNFNEETAVFGGSH
NLKLGLKNVTQQYKPQVIGIATTCLSETIGDDVPMILKDYKAIMGEADLPTMIFASTPSYTGSHIDGFHTAVRSAVQTFA
TGGAKKNMLNLFSGMISPADIRYLKEILKEFGMPFMLLPDYSQTLDGGPWGEYHRIPPGGTPTSAIADSGSAAASIEFGS
TLEAKKSAAGYLEETFGVPRHSMPLPIGIKATDRFFALLEELTEKPMPEKYEDERRRLVDAYADGHKYVFGRKAILYGEE
DLVISMAAFLREIGVVPVLCASGGKSGLMKKRMLELIPDLEELEIQVREGVDFVDIENEAKVLKPDFLIGNSKGFTMSRK
NELQLIRIGFPIHDRFGGQRLHHLGYKGTQELFDRIVNTVIEYRQNSSPVGYTYI
>Mature_454_residues
TTNASTMTAKTATQNACKLCTPLGACLAFRGIEKCVPFLHGSQGCATYIRRYLISHYKEPIDIASSNFNEETAVFGGSHN
LKLGLKNVTQQYKPQVIGIATTCLSETIGDDVPMILKDYKAIMGEADLPTMIFASTPSYTGSHIDGFHTAVRSAVQTFAT
GGAKKNMLNLFSGMISPADIRYLKEILKEFGMPFMLLPDYSQTLDGGPWGEYHRIPPGGTPTSAIADSGSAAASIEFGST
LEAKKSAAGYLEETFGVPRHSMPLPIGIKATDRFFALLEELTEKPMPEKYEDERRRLVDAYADGHKYVFGRKAILYGEED
LVISMAAFLREIGVVPVLCASGGKSGLMKKRMLELIPDLEELEIQVREGVDFVDIENEAKVLKPDFLIGNSKGFTMSRKN
ELQLIRIGFPIHDRFGGQRLHHLGYKGTQELFDRIVNTVIEYRQNSSPVGYTYI

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 50158; Mature: 50027

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNASTMTAKTATQNACKLCTPLGACLAFRGIEKCVPFLHGSQGCATYIRRYLISHYKE
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
PIDIASSNFNEETAVFGGSHNLKLGLKNVTQQYKPQVIGIATTCLSETIGDDVPMILKDY
CHHHHCCCCCCCCEEECCCCCEEECHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHHH
KAIMGEADLPTMIFASTPSYTGSHIDGFHTAVRSAVQTFATGGAKKNMLNLFSGMISPAD
HHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
IRYLKEILKEFGMPFMLLPDYSQTLDGGPWGEYHRIPPGGTPTSAIADSGSAAASIEFGS
HHHHHHHHHHHCCCEEECCCCHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCC
TLEAKKSAAGYLEETFGVPRHSMPLPIGIKATDRFFALLEELTEKPMPEKYEDERRRLVD
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AYADGHKYVFGRKAILYGEEDLVISMAAFLREIGVVPVLCASGGKSGLMKKRMLELIPDL
HHHCCCEEEEECEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCH
EELEIQVREGVDFVDIENEAKVLKPDFLIGNSKGFTMSRKNELQLIRIGFPIHDRFGGQR
HHHHHHHHCCCCEEEECCCCCEECCCEEEECCCCCEECCCCCEEEEEECCCHHHHCCCHH
LHHLGYKGTQELFDRIVNTVIEYRQNSSPVGYTYI
HHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECC
>Mature Secondary Structure 
TTNASTMTAKTATQNACKLCTPLGACLAFRGIEKCVPFLHGSQGCATYIRRYLISHYKE
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
PIDIASSNFNEETAVFGGSHNLKLGLKNVTQQYKPQVIGIATTCLSETIGDDVPMILKDY
CHHHHCCCCCCCCEEECCCCCEEECHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHHH
KAIMGEADLPTMIFASTPSYTGSHIDGFHTAVRSAVQTFATGGAKKNMLNLFSGMISPAD
HHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
IRYLKEILKEFGMPFMLLPDYSQTLDGGPWGEYHRIPPGGTPTSAIADSGSAAASIEFGS
HHHHHHHHHHHCCCEEECCCCHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCC
TLEAKKSAAGYLEETFGVPRHSMPLPIGIKATDRFFALLEELTEKPMPEKYEDERRRLVD
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AYADGHKYVFGRKAILYGEEDLVISMAAFLREIGVVPVLCASGGKSGLMKKRMLELIPDL
HHHCCCEEEEECEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCH
EELEIQVREGVDFVDIENEAKVLKPDFLIGNSKGFTMSRKNELQLIRIGFPIHDRFGGQR
HHHHHHHHCCCCEEEECCCCCEECCCEEEECCCCCEECCCCCEEEEEECCCHHHHCCCHH
LHHLGYKGTQELFDRIVNTVIEYRQNSSPVGYTYI
HHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550408; 7961410 [H]