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The map label for this gene is nifE [H]

Identifier: 193213267

GI number: 193213267

Start: 1775056

End: 1776441

Strand: Direct

Name: nifE [H]

Synonym: Cpar_1624

Alternate gene names: 193213267

Gene position: 1775056-1776441 (Clockwise)

Preceding gene: 193213266

Following gene: 193213268

Centisome position: 77.54

GC content: 56.93

Gene sequence:

>1386_bases
ATGAAGGGAAAGGAAGTAATGGATACGCAGAACATCAGTCTGCTTGAGGGCAGGCAAAAACAGGTCTACGAGAAGACCGC
GGGTGCTCCGGAAGGTGACATCGCGTGCGAAAAGACCAGCCTCTCCGGCTCGGTCAGCCAGCGGGCCTGCGTCTTCTGCG
GCTCACGTGTCGTGCTCTATCCGGTAGCCGACGCTATTCACATCGTACACGGCCCGATCGGTTGCGCCGCCTACACCTGG
GACATCCGGGGAGCGGTTTCGTCGGGACCCGAACTGCACAGGCTCAGCTTTTCATCCGACCTCCAGGAGATGGACGTCAT
CTACGGCGGCGAAAAGAAGCTCTACAAATCTCTGATCGAACTGATCGATCAATACCAACCCAAAGCGGCATTCATCTACT
CGACCTGCATCATCGGCCTCATCGGAGACGATATCGATGCGGTCTGCAAGAAAGTTGCCAAAGAGAAAGGAATCCCGGTG
CTTCCGGTGCATTCCGAAGGATTCAAGGGCACCAAGAAGGATGGCTACAAAGCCGCCTGCAACGCCCTCATGAAGCTGAT
CGGCCAGGGTTCGACCGAAGGCATCAGCAAATACAGCATTAACATTCTCGGCGAATTCAACCTCGCCGGAGAAGCCTGGA
TCATCCGGGAATATTACGAGAAAATGGGCATCCAGGTGGTCGCCACCATGACCGGCGACGGCCGCATCGACGACATCCGC
CGCTCGCACGGCGCTTCGCTGAACGTGGTGCAGTGCTCCGGCTCGATGACCACCCTTGCCAAAGAGATGGAAGAGAAGTA
CGGCATTCCCTACATCAGGGTCTCCTACTTCGGCTTCGAGGATATGTCCAAATCACTCTACGACGTAGCCCAGCACTTCC
CCGAAAGGCCCGACATTATGGAGAAGGCCAAGGAGATCGTGCGTGACGAGATCAAGCAATACTACCCCGAGATGCAGAAG
TTCAAAGCTGCCCTGACCGGGAAGAAAGCCGCGATCTACGTCGGCGGAGCCTTCAAGACCTTCTCGCTCATCAAAGCCCT
GCGCACCGTCGGCATGTCGGTGGTGCTGGCCGGTTCGCAGACCGGCAACAAGCAGGACTACAAAAACCTCAAGGAGATGT
GCGACGAGGGCACCGTCATCGTTGACGACTCCAACCCGGTCGAGCTCTCCAAGTTCATCCTTGAAAAAGAGGCTGACCTG
CTCATCGGCGGCGTCAAGGAGCGCCCTATCGCCTTCAAGCTCGGCGTCGCTTTCTGCGACCACAACCACGAGCGCAAGAT
TCCGCTCGCCGGATTCATCGGCATGTACTATTTCGCCAAGGAGGTTTACGAATCGGTGATGAGCCCCGTGTGGCAGTTCG
CCCCGAGAAAAGGAGGTGTCAAATGA

Upstream 100 bases:

>100_bases
AGAACACTGTCCGGCTCGTCCGCGAACCGCTCTCGACGACAACGTATCGACAAGTTGACAACACAACCATTCCCCTTCGC
CAAAACGGAGGGGGATGACA

Downstream 100 bases:

>100_bases
CCACGAACGCTTCGACCATGACGGCCAAAACGGCCACGCAGAACGCATGCAAGCTCTGCACTCCGCTCGGCGCGTGCCTG
GCCTTCCGCGGCATCGAAAA

Product: nitrogenase MoFe cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKGKEVMDTQNISLLEGRQKQVYEKTAGAPEGDIACEKTSLSGSVSQRACVFCGSRVVLYPVADAIHIVHGPIGCAAYTW
DIRGAVSSGPELHRLSFSSDLQEMDVIYGGEKKLYKSLIELIDQYQPKAAFIYSTCIIGLIGDDIDAVCKKVAKEKGIPV
LPVHSEGFKGTKKDGYKAACNALMKLIGQGSTEGISKYSINILGEFNLAGEAWIIREYYEKMGIQVVATMTGDGRIDDIR
RSHGASLNVVQCSGSMTTLAKEMEEKYGIPYIRVSYFGFEDMSKSLYDVAQHFPERPDIMEKAKEIVRDEIKQYYPEMQK
FKAALTGKKAAIYVGGAFKTFSLIKALRTVGMSVVLAGSQTGNKQDYKNLKEMCDEGTVIVDDSNPVELSKFILEKEADL
LIGGVKERPIAFKLGVAFCDHNHERKIPLAGFIGMYYFAKEVYESVMSPVWQFAPRKGGVK

Sequences:

>Translated_461_residues
MKGKEVMDTQNISLLEGRQKQVYEKTAGAPEGDIACEKTSLSGSVSQRACVFCGSRVVLYPVADAIHIVHGPIGCAAYTW
DIRGAVSSGPELHRLSFSSDLQEMDVIYGGEKKLYKSLIELIDQYQPKAAFIYSTCIIGLIGDDIDAVCKKVAKEKGIPV
LPVHSEGFKGTKKDGYKAACNALMKLIGQGSTEGISKYSINILGEFNLAGEAWIIREYYEKMGIQVVATMTGDGRIDDIR
RSHGASLNVVQCSGSMTTLAKEMEEKYGIPYIRVSYFGFEDMSKSLYDVAQHFPERPDIMEKAKEIVRDEIKQYYPEMQK
FKAALTGKKAAIYVGGAFKTFSLIKALRTVGMSVVLAGSQTGNKQDYKNLKEMCDEGTVIVDDSNPVELSKFILEKEADL
LIGGVKERPIAFKLGVAFCDHNHERKIPLAGFIGMYYFAKEVYESVMSPVWQFAPRKGGVK
>Mature_461_residues
MKGKEVMDTQNISLLEGRQKQVYEKTAGAPEGDIACEKTSLSGSVSQRACVFCGSRVVLYPVADAIHIVHGPIGCAAYTW
DIRGAVSSGPELHRLSFSSDLQEMDVIYGGEKKLYKSLIELIDQYQPKAAFIYSTCIIGLIGDDIDAVCKKVAKEKGIPV
LPVHSEGFKGTKKDGYKAACNALMKLIGQGSTEGISKYSINILGEFNLAGEAWIIREYYEKMGIQVVATMTGDGRIDDIR
RSHGASLNVVQCSGSMTTLAKEMEEKYGIPYIRVSYFGFEDMSKSLYDVAQHFPERPDIMEKAKEIVRDEIKQYYPEMQK
FKAALTGKKAAIYVGGAFKTFSLIKALRTVGMSVVLAGSQTGNKQDYKNLKEMCDEGTVIVDDSNPVELSKFILEKEADL
LIGGVKERPIAFKLGVAFCDHNHERKIPLAGFIGMYYFAKEVYESVMSPVWQFAPRKGGVK

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 50897; Mature: 50897

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKEVMDTQNISLLEGRQKQVYEKTAGAPEGDIACEKTSLSGSVSQRACVFCGSRVVLY
CCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHCCCCEEEE
PVADAIHIVHGPIGCAAYTWDIRGAVSSGPELHRLSFSSDLQEMDVIYGGEKKLYKSLIE
EHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHH
LIDQYQPKAAFIYSTCIIGLIGDDIDAVCKKVAKEKGIPVLPVHSEGFKGTKKDGYKAAC
HHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHH
NALMKLIGQGSTEGISKYSINILGEFNLAGEAWIIREYYEKMGIQVVATMTGDGRIDDIR
HHHHHHHCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCHHHHH
RSHGASLNVVQCSGSMTTLAKEMEEKYGIPYIRVSYFGFEDMSKSLYDVAQHFPERPDIM
HHCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCHH
EKAKEIVRDEIKQYYPEMQKFKAALTGKKAAIYVGGAFKTFSLIKALRTVGMSVVLAGSQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCC
TGNKQDYKNLKEMCDEGTVIVDDSNPVELSKFILEKEADLLIGGVKERPIAFKLGVAFCD
CCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEECCCCCCCEEEEEEEHEEC
HNHERKIPLAGFIGMYYFAKEVYESVMSPVWQFAPRKGGVK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKGKEVMDTQNISLLEGRQKQVYEKTAGAPEGDIACEKTSLSGSVSQRACVFCGSRVVLY
CCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHCCCCEEEE
PVADAIHIVHGPIGCAAYTWDIRGAVSSGPELHRLSFSSDLQEMDVIYGGEKKLYKSLIE
EHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHH
LIDQYQPKAAFIYSTCIIGLIGDDIDAVCKKVAKEKGIPVLPVHSEGFKGTKKDGYKAAC
HHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHH
NALMKLIGQGSTEGISKYSINILGEFNLAGEAWIIREYYEKMGIQVVATMTGDGRIDDIR
HHHHHHHCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCHHHHH
RSHGASLNVVQCSGSMTTLAKEMEEKYGIPYIRVSYFGFEDMSKSLYDVAQHFPERPDIM
HHCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCHH
EKAKEIVRDEIKQYYPEMQKFKAALTGKKAAIYVGGAFKTFSLIKALRTVGMSVVLAGSQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCC
TGNKQDYKNLKEMCDEGTVIVDDSNPVELSKFILEKEADLLIGGVKERPIAFKLGVAFCD
CCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEECCCCCCCEEEEEEEHEEC
HNHERKIPLAGFIGMYYFAKEVYESVMSPVWQFAPRKGGVK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2726466 [H]