Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 193213250

GI number: 193213250

Start: 1755001

End: 1755756

Strand: Direct

Name: hisF

Synonym: Cpar_1605

Alternate gene names: 193213250

Gene position: 1755001-1755756 (Clockwise)

Preceding gene: 193213239

Following gene: 193213253

Centisome position: 76.66

GC content: 57.41

Gene sequence:

>756_bases
ATGTTAGCCAAACGAATCATCCCCTGCCTCGACGTCCGCGACGGACGGGTTGTGAAAGGAATCAATTTCGAAGGTCTCCG
TGACGCGGGTTCGATTCTCGAACAGGCCCGGTTTTACAACAATGAAATGGCCGACGAACTGGTCTTTCTCGATATTTCCG
CATCCATCGAATCGAGAAGGACAACGCTTGAGGAGGTGCTGAAAGTTTCAGGCGAGGTGTTCATTCCACTGACGGTCGGC
GGAGGCATCAGCTCGGTCGAGCGGGCGCGCGACGCCTTTTTGCACGGTGCAGACAAGGTGTCGGTCAATACCGCTGCGGT
GAACGATCCAGTGCTGATTTCGCGCATCGCCGAGCGCTTCGGCTCGCAGGCTGTAGTGGTGGCGATTGACGTCAAGAAGG
TGAATGGTGAGTACATCGTGCACACCCATTCAGGCAAAACGCCGACGCAGTACGAGGCGCTGGAGTGGGCACACCGGGTG
CAGGAGCTTGGCGCGGGGGAAATTCTCTTGACCAGCATGGATCGCGACGGCACGCAGGAGGGCTACGACAACGAGATTCT
CAAACGCATCTCGACCTCGGTGCACATTCCGGTGATCGCTTCAGGCGGCGCGGGCAACCTCGAACATCTTTACGAAGGTT
TCACCAAAGGCCACGCCGATGCGGCGCTTGCGGCTTCGATCTTCCACTTCCGCACCTACTCCATCAGAGAGGCAAAAGAG
TACCTTCGCGACAAGGGTATTGCGGTCAGGCTTTGA

Upstream 100 bases:

>100_bases
GAAGACTTTAATATATTAGTTGCAGATTCAAAGTTTTGTGATTTCTTGAGTCAGGCACCGGCTCTACGACCGGATTTTCC
GACTCCCAAATTCTGCCAAG

Downstream 100 bases:

>100_bases
CCGCCTTGATCCAGTCGATCTGAAGGTTGAAATTGCCCTTCTGCTGCTCGGCCACAAGGAAGCAGATCTGAATCACGCTC
GACGAGTCGAACGGCGCGTC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNEMADELVFLDISASIESRRTTLEEVLKVSGEVFIPLTVG
GGISSVERARDAFLHGADKVSVNTAAVNDPVLISRIAERFGSQAVVVAIDVKKVNGEYIVHTHSGKTPTQYEALEWAHRV
QELGAGEILLTSMDRDGTQEGYDNEILKRISTSVHIPVIASGGAGNLEHLYEGFTKGHADAALAASIFHFRTYSIREAKE
YLRDKGIAVRL

Sequences:

>Translated_251_residues
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNEMADELVFLDISASIESRRTTLEEVLKVSGEVFIPLTVG
GGISSVERARDAFLHGADKVSVNTAAVNDPVLISRIAERFGSQAVVVAIDVKKVNGEYIVHTHSGKTPTQYEALEWAHRV
QELGAGEILLTSMDRDGTQEGYDNEILKRISTSVHIPVIASGGAGNLEHLYEGFTKGHADAALAASIFHFRTYSIREAKE
YLRDKGIAVRL
>Mature_251_residues
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNEMADELVFLDISASIESRRTTLEEVLKVSGEVFIPLTVG
GGISSVERARDAFLHGADKVSVNTAAVNDPVLISRIAERFGSQAVVVAIDVKKVNGEYIVHTHSGKTPTQYEALEWAHRV
QELGAGEILLTSMDRDGTQEGYDNEILKRISTSVHIPVIASGGAGNLEHLYEGFTKGHADAALAASIFHFRTYSIREAKE
YLRDKGIAVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=47.4708171206226, Blast_Score=233, Evalue=6e-63,
Organism=Escherichia coli, GI87082028, Length=212, Percent_Identity=25.9433962264151, Blast_Score=62, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.3503184713376, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_CHLP8 (B3QQ00)

Other databases:

- EMBL:   CP001099
- RefSeq:   YP_001999203.1
- ProteinModelPortal:   B3QQ00
- GeneID:   6420547
- GenomeReviews:   CP001099_GR
- KEGG:   cpc:Cpar_1605
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27494; Mature: 27494

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNEMADELVFLDISASIESRR
CCHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCHHHCEEEEEEECCCHHHHH
TTLEEVLKVSGEVFIPLTVGGGISSVERARDAFLHGADKVSVNTAAVNDPVLISRIAERF
HHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
GSQAVVVAIDVKKVNGEYIVHTHSGKTPTQYEALEWAHRVQELGAGEILLTSMDRDGTQE
CCCEEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDNEILKRISTSVHIPVIASGGAGNLEHLYEGFTKGHADAALAASIFHFRTYSIREAKE
CCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
YLRDKGIAVRL
HHHHCCCEEEC
>Mature Secondary Structure
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNEMADELVFLDISASIESRR
CCHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCHHHCEEEEEEECCCHHHHH
TTLEEVLKVSGEVFIPLTVGGGISSVERARDAFLHGADKVSVNTAAVNDPVLISRIAERF
HHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHH
GSQAVVVAIDVKKVNGEYIVHTHSGKTPTQYEALEWAHRVQELGAGEILLTSMDRDGTQE
CCCEEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDNEILKRISTSVHIPVIASGGAGNLEHLYEGFTKGHADAALAASIFHFRTYSIREAKE
CCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
YLRDKGIAVRL
HHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA