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The map label for this gene is gltK [H]

Identifier: 190894984

GI number: 190894984

Start: 703156

End: 703818

Strand: Reverse

Name: gltK [H]

Synonym: RHECIAT_PC0000650

Alternate gene names: 190894984

Gene position: 703818-703156 (Counterclockwise)

Preceding gene: 190894985

Following gene: 190894983

Centisome position: 64.48

GC content: 61.24

Gene sequence:

>663_bases
ATGAACTACACGTTCGATTTCAGCGCCGTCTCCATCGCCCCGCTGCTGCAGGGGCTGTGGGTTTCGCTGCAGCTGACGGC
GGCCGCGAACATCATCGGCATCGTTCTGGGCTTCGGATTGGCCCTGCTGATCATGAGCCCGATCAAGATCGTGCGGCTGC
CGTTCATGCTGTTCGTCGAATTCTTCCGCTGCACGCCGGCCATCGTCCAGATCGTCTGGATATTCTATTGCGTGCCGATG
CTGTTCGATGTCTTCCTCGATCCGATGACGATGGGCATTATGGCCCTCGGGCTCAATCTGATGGCGTTCAATGCCGAGGC
CTATCGTGCCTCGATCCAGGCGGTGCCGCGCGAACAGCTCGACGCCGGCATCGCGCTCGGCCTCAATCCGCTGCAGCGGG
TGCTCTACATCGTCTTCCCGACGGCGTTCCGCGCCTCGATCCCGGTGCTTTTGACCAACGGCATCGTCATCTTCCAGCAG
AGCGCGCTAGTCGCGATCGTGGCGATCCAGGACCTGATGTACCAGGGCAAGACGCTGGCGACGGAGACTTATCGTCCGAT
CGAGACCTTCACCGTCGTCGCCCTCATCTATTTCGCCGTGTCTTTCCCGATCACCCAGATCGTCGGCCTGCTCGAACGCC
GCCGCCAGATCCTCGTCAGCTAG

Upstream 100 bases:

>100_bases
CGAGGACGGCCCGCCCGAGCAGGTGCTCGGCGCTCCGCGCGAGGAACGCACCAAGGAATTCATCGACCGCGTCTTCCACA
TCAAGCAGTAGGAGGCACAG

Downstream 100 bases:

>100_bases
GAGGCCAGCATGATGACGCTCGATTTCTCGATCCTGCTGCGCTTCGAAAACGCGCTGCTCCTTGGCCTGTGGATGACGAT
CAAGCTGACGATCATCTGCG

Product: putative amino acid ABC transporter permease

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MNYTFDFSAVSIAPLLQGLWVSLQLTAAANIIGIVLGFGLALLIMSPIKIVRLPFMLFVEFFRCTPAIVQIVWIFYCVPM
LFDVFLDPMTMGIMALGLNLMAFNAEAYRASIQAVPREQLDAGIALGLNPLQRVLYIVFPTAFRASIPVLLTNGIVIFQQ
SALVAIVAIQDLMYQGKTLATETYRPIETFTVVALIYFAVSFPITQIVGLLERRRQILVS

Sequences:

>Translated_220_residues
MNYTFDFSAVSIAPLLQGLWVSLQLTAAANIIGIVLGFGLALLIMSPIKIVRLPFMLFVEFFRCTPAIVQIVWIFYCVPM
LFDVFLDPMTMGIMALGLNLMAFNAEAYRASIQAVPREQLDAGIALGLNPLQRVLYIVFPTAFRASIPVLLTNGIVIFQQ
SALVAIVAIQDLMYQGKTLATETYRPIETFTVVALIYFAVSFPITQIVGLLERRRQILVS
>Mature_220_residues
MNYTFDFSAVSIAPLLQGLWVSLQLTAAANIIGIVLGFGLALLIMSPIKIVRLPFMLFVEFFRCTPAIVQIVWIFYCVPM
LFDVFLDPMTMGIMALGLNLMAFNAEAYRASIQAVPREQLDAGIALGLNPLQRVLYIVFPTAFRASIPVLLTNGIVIFQQ
SALVAIVAIQDLMYQGKTLATETYRPIETFTVVALIYFAVSFPITQIVGLLERRRQILVS

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786873, Length=221, Percent_Identity=31.6742081447964, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787030, Length=223, Percent_Identity=29.5964125560538, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788226, Length=184, Percent_Identity=32.0652173913043, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788646, Length=226, Percent_Identity=26.1061946902655, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788645, Length=207, Percent_Identity=26.5700483091787, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786874, Length=212, Percent_Identity=28.3018867924528, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787087, Length=120, Percent_Identity=34.1666666666667, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787086, Length=214, Percent_Identity=26.6355140186916, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082239, Length=211, Percent_Identity=27.0142180094787, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 24430; Mature: 24430

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTFDFSAVSIAPLLQGLWVSLQLTAAANIIGIVLGFGLALLIMSPIKIVRLPFMLFVE
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFRCTPAIVQIVWIFYCVPMLFDVFLDPMTMGIMALGLNLMAFNAEAYRASIQAVPREQL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHCCHHHH
DAGIALGLNPLQRVLYIVFPTAFRASIPVLLTNGIVIFQQSALVAIVAIQDLMYQGKTLA
CCCHHCCCHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEECHHHHHHHHHHHHHHCCCHHH
TETYRPIETFTVVALIYFAVSFPITQIVGLLERRRQILVS
HCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYTFDFSAVSIAPLLQGLWVSLQLTAAANIIGIVLGFGLALLIMSPIKIVRLPFMLFVE
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFRCTPAIVQIVWIFYCVPMLFDVFLDPMTMGIMALGLNLMAFNAEAYRASIQAVPREQL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHCCHHHH
DAGIALGLNPLQRVLYIVFPTAFRASIPVLLTNGIVIFQQSALVAIVAIQDLMYQGKTLA
CCCHHCCCHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEECHHHHHHHHHHHHHHCCCHHH
TETYRPIETFTVVALIYFAVSFPITQIVGLLERRRQILVS
HCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]