Click here to switch to the map view.

The map label for this gene is prfB [H]

Identifier: 190571741

GI number: 190571741

Start: 1463197

End: 1464204

Strand: Reverse

Name: prfB [H]

Synonym: WPa_1375

Alternate gene names: 190571741

Gene position: 1464204-1463197 (Counterclockwise)

Preceding gene: 190571746

Following gene: 190571738

Centisome position: 98.77

GC content: 36.11

Gene sequence:

>1008_bases
TTGCGTCTTGAAGAGCTGGATTCTCAAGCGTCGAATGATAATCTGTGGCAAGATAATCAAAAAGCACAAGAGATTTTAAA
AGAACGCTCTAAAATTAAGCATGAAATAGAATCGTTTTCAAAGCTAGAAAGCGATTACAACGATTCTATCAGCTTGATGA
GATCTGCTATTGATGAGAATGATGAAGAATTTTTTTCTGAAGTTAAAGGTGAGTTAGTAAAGCTAGAGAAATTAATCAAA
CTTAAAGAGACAGAATCCTTATTTACTGGTGAAGCAGATAACAATAACTGCTTTTTAGAAATCCACTCAGGAGCCGGTGG
AACAGAGAGCAATGATTGGACTGAGATGCTCATGCGCATGTATACAAGATGGGCAGAAATTTATCACAATTTTAAAGTTG
AAGTTGTAGAAAAATTAGATGGTGATGCAGTTGGTATAAAATCTACAACAATAAAGATCATCGGAGAAAAAGCTTACGGG
TGGGCAAAAAGCGAAAGTGGAGTTCATAGATTGGTTAGAATATCACCATTTGATGCAAATGGTAAACGTCATACCAGTTT
TGCAAGTGTAGGAGTAACTCCAGTGATTGAAGATTCAATAGATATTGTTGTGGATGAAAAGGATTTAAAGATCGACACTT
ACCGTGCTTCTGGAGCAGGTGGTCAGCATGTCAATAAGACTGAAAGCGCGGTACGTATTACGCATATTCCAACAGGCGTT
GTAGCTCAATGCCAAAATGGTCGTTCTCAACATAAAAATAAAGATGAAGCACTTAAACTACTTAAAGGACGTTTATATCA
AATTGAATTGGAAAAAAAAGAACAGAAAATGGCCGAAGAATATGGCAAGAAATGCGATATAGGTTGGGGCAGTCAGATCA
GATCGTATGTTATGCATCCATATCAAATGGTAAAGGATTTAAGGACTGGACATGAAGTTGGCAATATAAATTCCGTTTTT
GATGGCAATATAGATTGTTTCATAGTTAGTGTATTGGAGTTAAAATGA

Upstream 100 bases:

>100_bases
AGTATAATGAAAACCCATCCAGAAATCCTTGAATACTTTCAAGGCTTAAATAAAAGTATTTCTCTTATCAGGAGGTGTCT
TTGACATAGAAAAATTGAAG

Downstream 100 bases:

>100_bases
GATTTATTAAAAAGCTAAGTTTTATGCTCTGCTAATAAGGGGAAAAGAAAACCAAAATCTATTGAGAAAAATGGAACAAT
TTTGTGAATATATCTATTAT

Product: protein chain release factor b

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MRLEELDSQASNDNLWQDNQKAQEILKERSKIKHEIESFSKLESDYNDSISLMRSAIDENDEEFFSEVKGELVKLEKLIK
LKETESLFTGEADNNNCFLEIHSGAGGTESNDWTEMLMRMYTRWAEIYHNFKVEVVEKLDGDAVGIKSTTIKIIGEKAYG
WAKSESGVHRLVRISPFDANGKRHTSFASVGVTPVIEDSIDIVVDEKDLKIDTYRASGAGGQHVNKTESAVRITHIPTGV
VAQCQNGRSQHKNKDEALKLLKGRLYQIELEKKEQKMAEEYGKKCDIGWGSQIRSYVMHPYQMVKDLRTGHEVGNINSVF
DGNIDCFIVSVLELK

Sequences:

>Translated_335_residues
MRLEELDSQASNDNLWQDNQKAQEILKERSKIKHEIESFSKLESDYNDSISLMRSAIDENDEEFFSEVKGELVKLEKLIK
LKETESLFTGEADNNNCFLEIHSGAGGTESNDWTEMLMRMYTRWAEIYHNFKVEVVEKLDGDAVGIKSTTIKIIGEKAYG
WAKSESGVHRLVRISPFDANGKRHTSFASVGVTPVIEDSIDIVVDEKDLKIDTYRASGAGGQHVNKTESAVRITHIPTGV
VAQCQNGRSQHKNKDEALKLLKGRLYQIELEKKEQKMAEEYGKKCDIGWGSQIRSYVMHPYQMVKDLRTGHEVGNINSVF
DGNIDCFIVSVLELK
>Mature_335_residues
MRLEELDSQASNDNLWQDNQKAQEILKERSKIKHEIESFSKLESDYNDSISLMRSAIDENDEEFFSEVKGELVKLEKLIK
LKETESLFTGEADNNNCFLEIHSGAGGTESNDWTEMLMRMYTRWAEIYHNFKVEVVEKLDGDAVGIKSTTIKIIGEKAYG
WAKSESGVHRLVRISPFDANGKRHTSFASVGVTPVIEDSIDIVVDEKDLKIDTYRASGAGGQHVNKTESAVRITHIPTGV
VAQCQNGRSQHKNKDEALKLLKGRLYQIELEKKEQKMAEEYGKKCDIGWGSQIRSYVMHPYQMVKDLRTGHEVGNINSVF
DGNIDCFIVSVLELK

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=248, Percent_Identity=37.5, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI34577120, Length=292, Percent_Identity=35.6164383561644, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI166795305, Length=148, Percent_Identity=41.2162162162162, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI2367172, Length=331, Percent_Identity=50.4531722054381, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1787462, Length=244, Percent_Identity=39.7540983606557, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=258, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=235, Percent_Identity=36.1702127659575, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19921226, Length=304, Percent_Identity=33.8815789473684, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37969; Mature: 37969

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEELDSQASNDNLWQDNQKAQEILKERSKIKHEIESFSKLESDYNDSISLMRSAIDEN
CCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
DEEFFSEVKGELVKLEKLIKLKETESLFTGEADNNNCFLEIHSGAGGTESNDWTEMLMRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
YTRWAEIYHNFKVEVVEKLDGDAVGIKSTTIKIIGEKAYGWAKSESGVHRLVRISPFDAN
HHHHHHHHHCCHHHHHHHCCCCEECCCCEEEEEEECHHCCCCCCCCCCEEEEEECCCCCC
GKRHTSFASVGVTPVIEDSIDIVVDEKDLKIDTYRASGAGGQHVNKTESAVRITHIPTGV
CCCCCCHHHCCCCEEECCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCHH
VAQCQNGRSQHKNKDEALKLLKGRLYQIELEKKEQKMAEEYGKKCDIGWGSQIRSYVMHP
HHHHHCCHHHCCCHHHHHHHHHCCEEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHCH
YQMVKDLRTGHEVGNINSVFDGNIDCFIVSVLELK
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MRLEELDSQASNDNLWQDNQKAQEILKERSKIKHEIESFSKLESDYNDSISLMRSAIDEN
CCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
DEEFFSEVKGELVKLEKLIKLKETESLFTGEADNNNCFLEIHSGAGGTESNDWTEMLMRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
YTRWAEIYHNFKVEVVEKLDGDAVGIKSTTIKIIGEKAYGWAKSESGVHRLVRISPFDAN
HHHHHHHHHCCHHHHHHHCCCCEECCCCEEEEEEECHHCCCCCCCCCCEEEEEECCCCCC
GKRHTSFASVGVTPVIEDSIDIVVDEKDLKIDTYRASGAGGQHVNKTESAVRITHIPTGV
CCCCCCHHHCCCCEEECCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCHH
VAQCQNGRSQHKNKDEALKLLKGRLYQIELEKKEQKMAEEYGKKCDIGWGSQIRSYVMHP
HHHHHCCHHHCCCHHHHHHHHHCCEEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHCH
YQMVKDLRTGHEVGNINSVFDGNIDCFIVSVLELK
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA