| Definition | Wolbachia endosymbiont of Culex quinquefasciatus Pel, complete genome. |
|---|---|
| Accession | NC_010981 |
| Length | 1,482,455 |
Click here to switch to the map view.
The map label for this gene is topA [H]
Identifier: 190571637
GI number: 190571637
Start: 1336502
End: 1338952
Strand: Reverse
Name: topA [H]
Synonym: WPa_1262
Alternate gene names: 190571637
Gene position: 1338952-1336502 (Counterclockwise)
Preceding gene: 190571641
Following gene: 190571636
Centisome position: 90.32
GC content: 36.52
Gene sequence:
>2451_bases ATGGCATTATTAATAGTTGAGTCGCCAGCAAAGGCAAAGACGATAGGTAAATATTTGAGTAAAGAATTCAAAGTGGCTGC ATCTTTTGGCCACGTCAGAGATCTGCCAGCAAAGAATGGTTCTGTTGATCCTGATAATGACTTTGCCATGAAGTATGAGA TTATTGAAAAAGCAGAAAAGTATGTAAAAGAGTTAGTCAAGGAAGCTAGTAAAACATCAGATATATACCTTGCAACAGAT CCAGACAGAGAAGGGGAAGCAATAGCTTGGAATGTGATAGAGGCACTAAAGGAAAGAAAAGCAATCAATGATGAAAGTAA CATTCATAGAGTAGTCTTTAACGAAATAACAAAAAGAGCAGTGCAAGAAGCTATAAAAAATCCACGTGAAATTAATATGG ACTTAGTGCGTGCACAGCAAGCACGCAGAGCTTTGGATTATCTAGTTGGATTTAGCTTGTCACCGCTGCTGTGGACAAAA TTGTCGGGAAGCAAATCTGCAGGGCGAGTGCAGTCTGTTGCATTAAAGCTTATATGCGAACGAGAAGATGAAATTAGTAA ATTTATAACACAGGAGTATTGGAGCATAAAGGCAGAAATGCAAAATAGCAAAGATGAGGCTTTTTTTGCTATGCTAAGCC ACTATGACAATAAAAAGCTAGAAAAATTTGATATTAAGAATGAAGAAGAGGCAAAGAACTTAGTCAGGGAGATCGAGTCA AGGCAGTATGCTGTAAGCACAGTAGAACGCAAGCAAGTTAAGAGAAACCCGCTTCCTCCATTTATCACCTCAAGTCTTCA GCAAGATGCAGTGAATAAACTGTATTTTAATGTGAAAAATGTTATGCGAGTAGCGCAAAATTTATATGAAGGTATCAATA TTGGTGGTGAAACCGTAGGGTTGATAACTTACATGCGTACAGATGGGTTTCATATTGCAGATGAGGCTATAAACTCAATC AGGGGATCAATTAAGTCATTATATGGTGATAAATATTTACCGCAGTCTCCTCGTAAATATGTAAAAAAGGTCAAAAATGC TCAAGAAGCACATGAAGCAATCCGGCCAACTGATATTAATAGAACACCGGGTAGTATTAAGGATTACTTAACGCCAGAGC AATTTAAATTATACGATTTAATCTGGAAAAGAACCATCGCAAGTCAAATGGAATCGGCGATTCTTGATCAAGTGGTAGTT GAAATTAGTTCTATTGACCAGAAAGTGATTCTGCGAGCAAGTGGATCAAGTATATTCTTTGATGGTTTTTATAAAGTTTA TCAAGATAACATAGAAGCCGAAAATGAAGGCCTGCTACCTGCCATGAAGGAAGAGGAAGCGTGCAAGTTGATTTCTGTTG ATCCGAAACAGCATTTTACTCAACCGCCACCTCGTTATAGTGAAGCGAGTATAGTAAAAAAAATGGAAGAAGTCGGTATA GGTCGCCCATCAACTTATGCAACAATTATTTCGGTATTACAAGATCGTGAATATGTTTCATTGGATAACAAAAGATTTAT TCCAAGCAGCCGCGGTAAGATCGTGACTATATTTTTGGAAACTTTTTTTCAGCGTTGTGTAGAGTATGACTTTACAGCAC AAATGGAAGAAAGGCTTGATTCAATCTCAAATGGACATGCAGATTGGAAAAAAGAACTAGGCTATTTTTGGGTGCCATTT TTTAATCATGTAAATTCTGTTAAGCAAATGACACATGATGAGATTTTTAGTGGTATTCATGATTTGGTAGTCAATTGGTT TTGCTCAGAGGAAGGAAAGGAAGAAATAGGTAAAAAATGTCCTAATTGCTCTGGTGGTATATTGAAATTAAATTTTGGAA AAGCCGGTGTGTTTCTTGGATGTTCTAACTATCCTGCATGCAATCATACAAAAGAAATTACGGGCAGTAATGACAATTCA GAATATCCAAAAAGTTTGGGTATAGATGATGTGACAGGCCAAGAGGTAATGATCAAAAAAGGTCCTTTTGGACTTTATCT GGAGTTTAATAGTGAGTCAGAAAAGAAAAAAAAGATTTCTGTACCAAAAGATATAAATGTTAATGATATTGATCTAAATA CCGCCACTCGATTACTTTCCCTGCCGAAAATAATCGGAGAGCACCCTGAAACTGGAAAAGAAGTAAAAATAGGTCTTGGA CGATTTGGGTACTATATTCTCTATGATGGTCGATACTTTTCTCTTAAAAAAAGCTCTAAGGAGGTATTAGACACACAATT AAACGAAGCTATACAAATTATTGAGAGCAGCCCACGCAAAGAGCTAAAATCTCTTGGTTTTAATGAAAAGGGAAAAGAAG TTTTTATCTGCAACGGTAGGTACGGATTCTATATAAAATGCGGTAAAACAAACGTTGCTTTGGGCAAGAATGCAGATATT GAAAGTATAGATCTGAAGAAGGCTTTAGAGTTAATTAAGAATAAAAAGTAG
Upstream 100 bases:
>100_bases ACATCAAACAATTACTTAGAAAAATTGGAAAGACCATTAAAATATTTTTTTACTTTTTATTCTATATATTTCAACATATT ACCTTTAAGTACTAATAATT
Downstream 100 bases:
>100_bases ACTTCTTGCATAACCATTCTTTATATGTTCTTTATTGTTGTTCCAGCGCTACACGCTGGAACTGAGAAAAAAAGAGACTG AATGACAGGAAAGAAGTTAC
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 816; Mature: 815
Protein sequence:
>816_residues MALLIVESPAKAKTIGKYLSKEFKVAASFGHVRDLPAKNGSVDPDNDFAMKYEIIEKAEKYVKELVKEASKTSDIYLATD PDREGEAIAWNVIEALKERKAINDESNIHRVVFNEITKRAVQEAIKNPREINMDLVRAQQARRALDYLVGFSLSPLLWTK LSGSKSAGRVQSVALKLICEREDEISKFITQEYWSIKAEMQNSKDEAFFAMLSHYDNKKLEKFDIKNEEEAKNLVREIES RQYAVSTVERKQVKRNPLPPFITSSLQQDAVNKLYFNVKNVMRVAQNLYEGINIGGETVGLITYMRTDGFHIADEAINSI RGSIKSLYGDKYLPQSPRKYVKKVKNAQEAHEAIRPTDINRTPGSIKDYLTPEQFKLYDLIWKRTIASQMESAILDQVVV EISSIDQKVILRASGSSIFFDGFYKVYQDNIEAENEGLLPAMKEEEACKLISVDPKQHFTQPPPRYSEASIVKKMEEVGI GRPSTYATIISVLQDREYVSLDNKRFIPSSRGKIVTIFLETFFQRCVEYDFTAQMEERLDSISNGHADWKKELGYFWVPF FNHVNSVKQMTHDEIFSGIHDLVVNWFCSEEGKEEIGKKCPNCSGGILKLNFGKAGVFLGCSNYPACNHTKEITGSNDNS EYPKSLGIDDVTGQEVMIKKGPFGLYLEFNSESEKKKKISVPKDINVNDIDLNTATRLLSLPKIIGEHPETGKEVKIGLG RFGYYILYDGRYFSLKKSSKEVLDTQLNEAIQIIESSPRKELKSLGFNEKGKEVFICNGRYGFYIKCGKTNVALGKNADI ESIDLKKALELIKNKK
Sequences:
>Translated_816_residues MALLIVESPAKAKTIGKYLSKEFKVAASFGHVRDLPAKNGSVDPDNDFAMKYEIIEKAEKYVKELVKEASKTSDIYLATD PDREGEAIAWNVIEALKERKAINDESNIHRVVFNEITKRAVQEAIKNPREINMDLVRAQQARRALDYLVGFSLSPLLWTK LSGSKSAGRVQSVALKLICEREDEISKFITQEYWSIKAEMQNSKDEAFFAMLSHYDNKKLEKFDIKNEEEAKNLVREIES RQYAVSTVERKQVKRNPLPPFITSSLQQDAVNKLYFNVKNVMRVAQNLYEGINIGGETVGLITYMRTDGFHIADEAINSI RGSIKSLYGDKYLPQSPRKYVKKVKNAQEAHEAIRPTDINRTPGSIKDYLTPEQFKLYDLIWKRTIASQMESAILDQVVV EISSIDQKVILRASGSSIFFDGFYKVYQDNIEAENEGLLPAMKEEEACKLISVDPKQHFTQPPPRYSEASIVKKMEEVGI GRPSTYATIISVLQDREYVSLDNKRFIPSSRGKIVTIFLETFFQRCVEYDFTAQMEERLDSISNGHADWKKELGYFWVPF FNHVNSVKQMTHDEIFSGIHDLVVNWFCSEEGKEEIGKKCPNCSGGILKLNFGKAGVFLGCSNYPACNHTKEITGSNDNS EYPKSLGIDDVTGQEVMIKKGPFGLYLEFNSESEKKKKISVPKDINVNDIDLNTATRLLSLPKIIGEHPETGKEVKIGLG RFGYYILYDGRYFSLKKSSKEVLDTQLNEAIQIIESSPRKELKSLGFNEKGKEVFICNGRYGFYIKCGKTNVALGKNADI ESIDLKKALELIKNKK >Mature_815_residues ALLIVESPAKAKTIGKYLSKEFKVAASFGHVRDLPAKNGSVDPDNDFAMKYEIIEKAEKYVKELVKEASKTSDIYLATDP DREGEAIAWNVIEALKERKAINDESNIHRVVFNEITKRAVQEAIKNPREINMDLVRAQQARRALDYLVGFSLSPLLWTKL SGSKSAGRVQSVALKLICEREDEISKFITQEYWSIKAEMQNSKDEAFFAMLSHYDNKKLEKFDIKNEEEAKNLVREIESR QYAVSTVERKQVKRNPLPPFITSSLQQDAVNKLYFNVKNVMRVAQNLYEGINIGGETVGLITYMRTDGFHIADEAINSIR GSIKSLYGDKYLPQSPRKYVKKVKNAQEAHEAIRPTDINRTPGSIKDYLTPEQFKLYDLIWKRTIASQMESAILDQVVVE ISSIDQKVILRASGSSIFFDGFYKVYQDNIEAENEGLLPAMKEEEACKLISVDPKQHFTQPPPRYSEASIVKKMEEVGIG RPSTYATIISVLQDREYVSLDNKRFIPSSRGKIVTIFLETFFQRCVEYDFTAQMEERLDSISNGHADWKKELGYFWVPFF NHVNSVKQMTHDEIFSGIHDLVVNWFCSEEGKEEIGKKCPNCSGGILKLNFGKAGVFLGCSNYPACNHTKEITGSNDNSE YPKSLGIDDVTGQEVMIKKGPFGLYLEFNSESEKKKKISVPKDINVNDIDLNTATRLLSLPKIIGEHPETGKEVKIGLGR FGYYILYDGRYFSLKKSSKEVLDTQLNEAIQIIESSPRKELKSLGFNEKGKEVFICNGRYGFYIKCGKTNVALGKNADIE SIDLKKALELIKNKK
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI4507635, Length=628, Percent_Identity=22.9299363057325, Blast_Score=117, Evalue=3e-26, Organism=Homo sapiens, GI10835218, Length=600, Percent_Identity=23.5, Blast_Score=108, Evalue=3e-23, Organism=Escherichia coli, GI1787529, Length=654, Percent_Identity=41.743119266055, Blast_Score=484, Evalue=1e-137, Organism=Escherichia coli, GI1788061, Length=622, Percent_Identity=24.7588424437299, Blast_Score=116, Evalue=7e-27, Organism=Caenorhabditis elegans, GI32563869, Length=556, Percent_Identity=25, Blast_Score=133, Evalue=4e-31, Organism=Caenorhabditis elegans, GI17555378, Length=521, Percent_Identity=24.1842610364683, Blast_Score=109, Evalue=6e-24, Organism=Saccharomyces cerevisiae, GI6323263, Length=560, Percent_Identity=22.8571428571429, Blast_Score=96, Evalue=3e-20,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR006171 [H]
Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 92604; Mature: 92473
Theoretical pI: Translated: 8.06; Mature: 8.06
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MALLIVESPAKAKTIGKYLSKEFKVAASFGHVRDLPAKNGSVDPDNDFAMKYEIIEKAEK CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHH YVKELVKEASKTSDIYLATDPDREGEAIAWNVIEALKERKAINDESNIHRVVFNEITKRA HHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH VQEAIKNPREINMDLVRAQQARRALDYLVGFSLSPLLWTKLSGSKSAGRVQSVALKLICE HHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCHHHHHHHHHHHHC REDEISKFITQEYWSIKAEMQNSKDEAFFAMLSHYDNKKLEKFDIKNEEEAKNLVREIES CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHH RQYAVSTVERKQVKRNPLPPFITSSLQQDAVNKLYFNVKNVMRVAQNLYEGINIGGETVG HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE LITYMRTDGFHIADEAINSIRGSIKSLYGDKYLPQSPRKYVKKVKNAQEAHEAIRPTDIN EEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC RTPGSIKDYLTPEQFKLYDLIWKRTIASQMESAILDQVVVEISSIDQKVILRASGSSIFF CCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEE DGFYKVYQDNIEAENEGLLPAMKEEEACKLISVDPKQHFTQPPPRYSEASIVKKMEEVGI HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCCCCHHHHHHHHHHHCC GRPSTYATIISVLQDREYVSLDNKRFIPSSRGKIVTIFLETFFQRCVEYDFTAQMEERLD CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHH SISNGHADWKKELGYFWVPFFNHVNSVKQMTHDEIFSGIHDLVVNWFCSEEGKEEIGKKC HHCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC PNCSGGILKLNFGKAGVFLGCSNYPACNHTKEITGSNDNSEYPKSLGIDDVTGQEVMIKK CCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE GPFGLYLEFNSESEKKKKISVPKDINVNDIDLNTATRLLSLPKIIGEHPETGKEVKIGLG CCEEEEEEECCCCCHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCEEEEEEC RFGYYILYDGRYFSLKKSSKEVLDTQLNEAIQIIESSPRKELKSLGFNEKGKEVFICNGR CCEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCC YGFYIKCGKTNVALGKNADIESIDLKKALELIKNKK EEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHCCC >Mature Secondary Structure ALLIVESPAKAKTIGKYLSKEFKVAASFGHVRDLPAKNGSVDPDNDFAMKYEIIEKAEK EEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHH YVKELVKEASKTSDIYLATDPDREGEAIAWNVIEALKERKAINDESNIHRVVFNEITKRA HHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH VQEAIKNPREINMDLVRAQQARRALDYLVGFSLSPLLWTKLSGSKSAGRVQSVALKLICE HHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCHHHHHHHHHHHHC REDEISKFITQEYWSIKAEMQNSKDEAFFAMLSHYDNKKLEKFDIKNEEEAKNLVREIES CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHH RQYAVSTVERKQVKRNPLPPFITSSLQQDAVNKLYFNVKNVMRVAQNLYEGINIGGETVG HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE LITYMRTDGFHIADEAINSIRGSIKSLYGDKYLPQSPRKYVKKVKNAQEAHEAIRPTDIN EEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC RTPGSIKDYLTPEQFKLYDLIWKRTIASQMESAILDQVVVEISSIDQKVILRASGSSIFF CCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEE DGFYKVYQDNIEAENEGLLPAMKEEEACKLISVDPKQHFTQPPPRYSEASIVKKMEEVGI HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCCCCHHHHHHHHHHHCC GRPSTYATIISVLQDREYVSLDNKRFIPSSRGKIVTIFLETFFQRCVEYDFTAQMEERLD CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHH SISNGHADWKKELGYFWVPFFNHVNSVKQMTHDEIFSGIHDLVVNWFCSEEGKEEIGKKC HHCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC PNCSGGILKLNFGKAGVFLGCSNYPACNHTKEITGSNDNSEYPKSLGIDDVTGQEVMIKK CCCCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE GPFGLYLEFNSESEKKKKISVPKDINVNDIDLNTATRLLSLPKIIGEHPETGKEVKIGLG CCEEEEEEECCCCCHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCEEEEEEC RFGYYILYDGRYFSLKKSSKEVLDTQLNEAIQIIESSPRKELKSLGFNEKGKEVFICNGR CCEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCC YGFYIKCGKTNVALGKNADIESIDLKKALELIKNKK EEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA