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The map label for this gene is pyrG [H]

Identifier: 190571612

GI number: 190571612

Start: 1307576

End: 1309183

Strand: Reverse

Name: pyrG [H]

Synonym: WPa_1235

Alternate gene names: 190571612

Gene position: 1309183-1307576 (Counterclockwise)

Preceding gene: 190571613

Following gene: 190571611

Centisome position: 88.31

GC content: 35.26

Gene sequence:

>1608_bases
ATGAAAGAAACCAAGTTTATCTTTGTTACAGGTGGAGTTGTTTCATCACTTGGTAAGGGTTTAGTTGCTTCAAGTGTGGG
AGCACTTCTTCAAGCTCATGGATTCAATGTTCGCATCAGAAAGCTTGACCCATATCTCAACATTGATCCTGGAACAATGA
GCCCAGCTCAGCACGGGGAAGTATTTGTTACTGAGGATGGTGCTGAAACTGATTTAGACCTTGGACATTACGAGCGTTTT
ACTAAAATTAAAGCAACCAAGGACGACAATATAACAACTGGTAAAGTATATAATAAATTATTAAAGAAGGAAAGGTGTGG
TGATTATCTAGGCAAAACTGTGCAAATCATTCCTCATGTGACAGATTTAATCAAATCTTTCATTTTTAATGGTACAGAAG
ATTTAGATTTTGTAATATGCGAAATAGGCGGAACCGTAGGTGATATTGAAAGCCAACCGTTTTTGGAAGCTATACGCCAA
GTTAATTATAAACTAGGAAAGCAAAAAGTTATCCTTATTCACTTAACTTTAATACCATATCTCACTGCAGCACAAGAATT
AAAGACAAAACCGACACAGCATTCAGTTCGAGAGTTAAACTCTGCGGGGTTACAACCAGATATTATATTATGTCGCAGTG
AAAAAGAAATTTCCGATAATCAAAAAGAAAAGATAGCCAATCTTTGCAATGTTTCTTTATCTAACGTAATACCTGCTCCT
GATGTAAACCATATATACGAGTTACCGTCCTTGTATAATCAATGTGGGCTCGGGGCACAAATTTTGGATCACTTTCACTT
AAGTAAGCCAAAACCAAGCTTACCTGAATGGGACCAAATAGTACACTCTATGAAACATCCAACACAAGAAGTTATCGTTT
CCATAGTAGGAAAATATACTGAATTTCCTGACGCATATAAATCACTGATTGAAGCATTAAACCATGGTGCAATTAGTAAT
AAAGCTAGAGTGAAGATAAATTGGGTCAACTCAAGAGAAGAGAATGGAAAATCTATAGATGCAAAGTTTATCAGAGAAAA
ATTACAAAGCTCTAATTCAGTCCTTGTTCCAGGAGGATTTGGTGATAATGGAATAGAAGGTAAAATATTAGCAATAAACT
ATGCCCGTGTAAATAACATTCCTTTTTTAGGAATATGTCTTGGTATGCAGCTTGCAGTGATTGAATTTGCTCGTAACGTT
ATTAAGCTTGAAGATGTACACTCTGAAGAATTTTATACTTGCAAACACCCAATCATCAAGCTCGCTGATGGTAAGAACGT
TGATCTTGGTGGAACCATGAGACTTGGAACATACAAGTGTAATGTAAATCCAAATTCAAAAATGGCAAATGTGTATAGTA
GTGTCACTATTTCAGAAAGACACAGACACAGATATATAGTTAATTTAGATTATAAAAATAACTTAGAAGAGAATGGAATG
ATATGCAGTGGTATATCAGAAGATAAAACATGCATAGAGGCAGTGGAGCTAAAAAATCACTCATGGTTTATTGGTGTGCA
ATTTCATCCAGAATTTCAATCAAGACCATTTTCTCCTCATCCTCTCTTTACATCATTTATTAAGGCAACTATTGACAATA
GAGTTTAA

Upstream 100 bases:

>100_bases
GATTATGTTCAAAAGATGTACATAAAAAATCAATTGCAGAGAAGATTATATCAGAAAAGAAGCAAGAAGGCCAATCAACT
TCTGTTCCATTTGAAAATTA

Downstream 100 bases:

>100_bases
TAGAACTTAATAAACTCTATTCTAATAGAGAGGTAAAAATGTTACTAAGAAATAGTAAAGATAGCATTACATATGGATCT
GCGACCTTCGAGGAATTGAA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MKETKFIFVTGGVVSSLGKGLVASSVGALLQAHGFNVRIRKLDPYLNIDPGTMSPAQHGEVFVTEDGAETDLDLGHYERF
TKIKATKDDNITTGKVYNKLLKKERCGDYLGKTVQIIPHVTDLIKSFIFNGTEDLDFVICEIGGTVGDIESQPFLEAIRQ
VNYKLGKQKVILIHLTLIPYLTAAQELKTKPTQHSVRELNSAGLQPDIILCRSEKEISDNQKEKIANLCNVSLSNVIPAP
DVNHIYELPSLYNQCGLGAQILDHFHLSKPKPSLPEWDQIVHSMKHPTQEVIVSIVGKYTEFPDAYKSLIEALNHGAISN
KARVKINWVNSREENGKSIDAKFIREKLQSSNSVLVPGGFGDNGIEGKILAINYARVNNIPFLGICLGMQLAVIEFARNV
IKLEDVHSEEFYTCKHPIIKLADGKNVDLGGTMRLGTYKCNVNPNSKMANVYSSVTISERHRHRYIVNLDYKNNLEENGM
ICSGISEDKTCIEAVELKNHSWFIGVQFHPEFQSRPFSPHPLFTSFIKATIDNRV

Sequences:

>Translated_535_residues
MKETKFIFVTGGVVSSLGKGLVASSVGALLQAHGFNVRIRKLDPYLNIDPGTMSPAQHGEVFVTEDGAETDLDLGHYERF
TKIKATKDDNITTGKVYNKLLKKERCGDYLGKTVQIIPHVTDLIKSFIFNGTEDLDFVICEIGGTVGDIESQPFLEAIRQ
VNYKLGKQKVILIHLTLIPYLTAAQELKTKPTQHSVRELNSAGLQPDIILCRSEKEISDNQKEKIANLCNVSLSNVIPAP
DVNHIYELPSLYNQCGLGAQILDHFHLSKPKPSLPEWDQIVHSMKHPTQEVIVSIVGKYTEFPDAYKSLIEALNHGAISN
KARVKINWVNSREENGKSIDAKFIREKLQSSNSVLVPGGFGDNGIEGKILAINYARVNNIPFLGICLGMQLAVIEFARNV
IKLEDVHSEEFYTCKHPIIKLADGKNVDLGGTMRLGTYKCNVNPNSKMANVYSSVTISERHRHRYIVNLDYKNNLEENGM
ICSGISEDKTCIEAVELKNHSWFIGVQFHPEFQSRPFSPHPLFTSFIKATIDNRV
>Mature_535_residues
MKETKFIFVTGGVVSSLGKGLVASSVGALLQAHGFNVRIRKLDPYLNIDPGTMSPAQHGEVFVTEDGAETDLDLGHYERF
TKIKATKDDNITTGKVYNKLLKKERCGDYLGKTVQIIPHVTDLIKSFIFNGTEDLDFVICEIGGTVGDIESQPFLEAIRQ
VNYKLGKQKVILIHLTLIPYLTAAQELKTKPTQHSVRELNSAGLQPDIILCRSEKEISDNQKEKIANLCNVSLSNVIPAP
DVNHIYELPSLYNQCGLGAQILDHFHLSKPKPSLPEWDQIVHSMKHPTQEVIVSIVGKYTEFPDAYKSLIEALNHGAISN
KARVKINWVNSREENGKSIDAKFIREKLQSSNSVLVPGGFGDNGIEGKILAINYARVNNIPFLGICLGMQLAVIEFARNV
IKLEDVHSEEFYTCKHPIIKLADGKNVDLGGTMRLGTYKCNVNPNSKMANVYSSVTISERHRHRYIVNLDYKNNLEENGM
ICSGISEDKTCIEAVELKNHSWFIGVQFHPEFQSRPFSPHPLFTSFIKATIDNRV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=547, Percent_Identity=44.0585009140768, Blast_Score=461, Evalue=1e-130,
Organism=Homo sapiens, GI28559085, Length=549, Percent_Identity=42.8051001821494, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI28559083, Length=549, Percent_Identity=42.8051001821494, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI221316689, Length=549, Percent_Identity=42.8051001821494, Blast_Score=440, Evalue=1e-123,
Organism=Escherichia coli, GI1789142, Length=540, Percent_Identity=50.9259259259259, Blast_Score=561, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI25148299, Length=604, Percent_Identity=37.9139072847682, Blast_Score=399, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6319432, Length=562, Percent_Identity=41.4590747330961, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6322563, Length=563, Percent_Identity=41.0301953818828, Blast_Score=425, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24664469, Length=549, Percent_Identity=44.8087431693989, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI21357815, Length=493, Percent_Identity=43.4077079107505, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59714; Mature: 59714

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETKFIFVTGGVVSSLGKGLVASSVGALLQAHGFNVRIRKLDPYLNIDPGTMSPAQHGE
CCCCEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCC
VFVTEDGAETDLDLGHYERFTKIKATKDDNITTGKVYNKLLKKERCGDYLGKTVQIIPHV
EEEECCCCCCCCCCHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TDLIKSFIFNGTEDLDFVICEIGGTVGDIESQPFLEAIRQVNYKLGKQKVILIHLTLIPY
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHH
LTAAQELKTKPTQHSVRELNSAGLQPDIILCRSEKEISDNQKEKIANLCNVSLSNVIPAP
HHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHCCCCC
DVNHIYELPSLYNQCGLGAQILDHFHLSKPKPSLPEWDQIVHSMKHPTQEVIVSIVGKYT
CHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHC
EFPDAYKSLIEALNHGAISNKARVKINWVNSREENGKSIDAKFIREKLQSSNSVLVPGGF
CCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEECCCC
GDNGIEGKILAINYARVNNIPFLGICLGMQLAVIEFARNVIKLEDVHSEEFYTCKHPIIK
CCCCCCCEEEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHEEHHHCCCCCEEECCCCEEE
LADGKNVDLGGTMRLGTYKCNVNPNSKMANVYSSVTISERHRHRYIVNLDYKNNLEENGM
ECCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCEEECCCCCEEEEEECCCCCCCCCCE
ICSGISEDKTCIEAVELKNHSWFIGVQFHPEFQSRPFSPHPLFTSFIKATIDNRV
EEECCCCCHHHHHHHHCCCCEEEEEEEECCHHCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKETKFIFVTGGVVSSLGKGLVASSVGALLQAHGFNVRIRKLDPYLNIDPGTMSPAQHGE
CCCCEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCC
VFVTEDGAETDLDLGHYERFTKIKATKDDNITTGKVYNKLLKKERCGDYLGKTVQIIPHV
EEEECCCCCCCCCCHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TDLIKSFIFNGTEDLDFVICEIGGTVGDIESQPFLEAIRQVNYKLGKQKVILIHLTLIPY
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHH
LTAAQELKTKPTQHSVRELNSAGLQPDIILCRSEKEISDNQKEKIANLCNVSLSNVIPAP
HHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHCCHHHHCCCCC
DVNHIYELPSLYNQCGLGAQILDHFHLSKPKPSLPEWDQIVHSMKHPTQEVIVSIVGKYT
CHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHC
EFPDAYKSLIEALNHGAISNKARVKINWVNSREENGKSIDAKFIREKLQSSNSVLVPGGF
CCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEECCCC
GDNGIEGKILAINYARVNNIPFLGICLGMQLAVIEFARNVIKLEDVHSEEFYTCKHPIIK
CCCCCCCEEEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHEEHHHCCCCCEEECCCCEEE
LADGKNVDLGGTMRLGTYKCNVNPNSKMANVYSSVTISERHRHRYIVNLDYKNNLEENGM
ECCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCEEECCCCCEEEEEECCCCCCCCCCE
ICSGISEDKTCIEAVELKNHSWFIGVQFHPEFQSRPFSPHPLFTSFIKATIDNRV
EEECCCCCHHHHHHHHCCCCEEEEEEEECCHHCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA