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The map label for this gene is ubiE [H]

Identifier: 190571599

GI number: 190571599

Start: 1295455

End: 1296153

Strand: Direct

Name: ubiE [H]

Synonym: WPa_1222

Alternate gene names: 190571599

Gene position: 1295455-1296153 (Clockwise)

Preceding gene: 190571595

Following gene: 190571600

Centisome position: 87.39

GC content: 33.48

Gene sequence:

>699_bases
ATGGATAAATCACAACTAGTTAAAGAGGTTTTCGATTCTGTAGCAAATTGCTACGACATCATGAATGATATAATGAGTCT
TGGAATGCACAGGCTATGGAAAGAGAAGATGGTAAGTAGTGTGCATTTTAAAAAGAACTCCAAGGTTTTGGATATTGCTG
GAGGAACCGGAGATATAGCTATAAGAATAGCAAGAGGTGAACCAAGTGCTCAAGTTACAGTATGTGATATAAATCAAAAC
ATGCTGGACAAAGGGCGTGATAAAGCAATAAATTCAAATCAGCTAAATTTCAATTGGGTATGTGCAAATGCAGAGAGCTT
ACCATTTGAAGATTCTGAATTTGATTATTATACAGTAGCTTTTGGTATTCGAAACTTTTCTGACCGTAAAAAGGCTTTAA
GTGAAGCATATAGGGTATTGAAGCAAAATGGACAATTTGTCTGCTTGGAATTTGCCCCTATGCATTATCAAAATGAGATA
TTCACCAAATTTTATGACTTATACTCATTTAAAGTAATTCCTAAAATTGGCAGCATAGTTGCTAAAGATAAGGGTTCTTA
TGAATATCTAGTAAAAAGCATAAGAGAATTTCCAACTCAGACTAACTTTAAAATGGAAATTGAAGAGGTAGGCTTTAAGA
ATGTTGAGTTCCACAATATGAGTTATGGAATAGTAGCATTACATATTGGAACAAAATAA

Upstream 100 bases:

>100_bases
CTGGATTAAATATGTTTAATTGTAGTATATTTTTCTCATTAGTATATTAATTTAACTGCTAAAGTTTAAAATAATTATGT
AAGCAATCTCTATATAAAAT

Downstream 100 bases:

>100_bases
ACTCTGGTCTATTCAGTAGTTTTTTTGCTAACTTTCTGTAATTTCGCAGCGTTTTTCCTTCAGAAGCTAAAGTTTCTTGA
ACGTATTGTTCTAAGGAAGT

Product: ubiquinone/menaquinone biosynthesis methlytransferase UbiE

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MDKSQLVKEVFDSVANCYDIMNDIMSLGMHRLWKEKMVSSVHFKKNSKVLDIAGGTGDIAIRIARGEPSAQVTVCDINQN
MLDKGRDKAINSNQLNFNWVCANAESLPFEDSEFDYYTVAFGIRNFSDRKKALSEAYRVLKQNGQFVCLEFAPMHYQNEI
FTKFYDLYSFKVIPKIGSIVAKDKGSYEYLVKSIREFPTQTNFKMEIEEVGFKNVEFHNMSYGIVALHIGTK

Sequences:

>Translated_232_residues
MDKSQLVKEVFDSVANCYDIMNDIMSLGMHRLWKEKMVSSVHFKKNSKVLDIAGGTGDIAIRIARGEPSAQVTVCDINQN
MLDKGRDKAINSNQLNFNWVCANAESLPFEDSEFDYYTVAFGIRNFSDRKKALSEAYRVLKQNGQFVCLEFAPMHYQNEI
FTKFYDLYSFKVIPKIGSIVAKDKGSYEYLVKSIREFPTQTNFKMEIEEVGFKNVEFHNMSYGIVALHIGTK
>Mature_232_residues
MDKSQLVKEVFDSVANCYDIMNDIMSLGMHRLWKEKMVSSVHFKKNSKVLDIAGGTGDIAIRIARGEPSAQVTVCDINQN
MLDKGRDKAINSNQLNFNWVCANAESLPFEDSEFDYYTVAFGIRNFSDRKKALSEAYRVLKQNGQFVCLEFAPMHYQNEI
FTKFYDLYSFKVIPKIGSIVAKDKGSYEYLVKSIREFPTQTNFKMEIEEVGFKNVEFHNMSYGIVALHIGTK

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=266, Percent_Identity=43.2330827067669, Blast_Score=222, Evalue=2e-58,
Organism=Escherichia coli, GI48994983, Length=234, Percent_Identity=46.5811965811966, Blast_Score=215, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17557039, Length=234, Percent_Identity=49.1452991452991, Blast_Score=237, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6323526, Length=247, Percent_Identity=42.1052631578947, Blast_Score=183, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24641702, Length=246, Percent_Identity=47.9674796747967, Blast_Score=234, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26586; Mature: 26586

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS01183 UBIE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSQLVKEVFDSVANCYDIMNDIMSLGMHRLWKEKMVSSVHFKKNSKVLDIAGGTGDIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEE
IRIARGEPSAQVTVCDINQNMLDKGRDKAINSNQLNFNWVCANAESLPFEDSEFDYYTVA
EEEECCCCCCEEEEEECCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEE
FGIRNFSDRKKALSEAYRVLKQNGQFVCLEFAPMHYQNEIFTKFYDLYSFKVIPKIGSIV
EECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEHHHHHHEE
AKDKGSYEYLVKSIREFPTQTNFKMEIEEVGFKNVEFHNMSYGIVALHIGTK
ECCCCCHHHHHHHHHHCCCCCCCEEEHHHCCCCCEEEECCCEEEEEEEECCC
>Mature Secondary Structure
MDKSQLVKEVFDSVANCYDIMNDIMSLGMHRLWKEKMVSSVHFKKNSKVLDIAGGTGDIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEE
IRIARGEPSAQVTVCDINQNMLDKGRDKAINSNQLNFNWVCANAESLPFEDSEFDYYTVA
EEEECCCCCCEEEEEECCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEE
FGIRNFSDRKKALSEAYRVLKQNGQFVCLEFAPMHYQNEIFTKFYDLYSFKVIPKIGSIV
EECCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEHHHHHHEE
AKDKGSYEYLVKSIREFPTQTNFKMEIEEVGFKNVEFHNMSYGIVALHIGTK
ECCCCCHHHHHHHHHHCCCCCCCEEEHHHCCCCCEEEECCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA