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The map label for this gene is iscS [H]

Identifier: 190571407

GI number: 190571407

Start: 1082446

End: 1083687

Strand: Direct

Name: iscS [H]

Synonym: WPa_1015

Alternate gene names: 190571407

Gene position: 1082446-1083687 (Clockwise)

Preceding gene: 190571406

Following gene: 190571410

Centisome position: 73.02

GC content: 37.52

Gene sequence:

>1242_bases
ATGAATATGGAAAAAATACGCAATGCTAAAATAAAATTACCAATATTTCTCGATTATCAATCTACTACTAAAGTAGATCC
TCGTGTTTTGGAGGTGATGATACCTTATTTTGGTGAGTTTAGTAATGCACACTCTCGTAGTCATTCATTTGGTTGGACAG
CTGAGGAAGCAGTAGAAAAAGCAAGGAAGCACATTGCTGATTTAGTGAATGCAGACAGCAAAGAAATCGTCTTTACCTCG
GGTGCAACCGAATCAAATAACATGGCTATCAAAGGTGTTGCTCATTTTTATAAGAATAAGGGAAATCATATAATAACTGT
CTGCACGGAGCATAAGTGCGTTTTAGATTCTTGTCGACATCTGGAAAATGAAGGCTTTAAAGTTACATATTTGTCCGTTA
AGCAAAACGGAATTATAGATTTGTTGAAACTTGAGGAGGCAATCACTGATAAAACAATTCTGATTTCAGTGATGATGGCA
AATAATGAGATCGGAGTAATGCAACCTGTCAAAGAAATTGGTGCAATGTGTAGAAAGCATAATATATTTTTTCATACAGA
CGCTGCTCAAAGCTTTGGAAAAGTTCCAATAGACGTAAATGAAATGAACATTGATCTTATGAGCCTTTCTAGCCATAAAA
TTTATGGACCTATGGGAATAGGTGCATTATATGTCCGTAGAAAAAATCCTCGTGTTAGGTTGACGCCACTAATTAGTGGT
GGTGGACAGGAGAGAGGCATGCGCTCTGGAACAGTTCCTACTCCCCTTGCAGTTGGTTTTGGTGAAGCGGCACGTATCGC
TAAAGAAGAAATGGAAATAGAAGCAAGCAAATTAGAAGAGTTGAGAGATATTTTGTACAACAAAATAAAAGAGGCATTTC
CTGATGTTGTTTTAAATGGTGACTACGAAAATAGGATTCCAGGTAACCTTAACTTAAGTTTTCCTTATGTTGAGGGTGAG
TCTCTTATCATGGCAATCAAGGATTTAGCAGTAAGCTCTGGTTCTGCATGCACATCTGCATCTCTTGAACCTTCTTATGT
CATAAGATCACTAAACAATGGCCACGATCTTGAGCATTCATCAATTAGATTTGGCCTTGGCCGATTTACAACTAAAGATG
AAGTTCTATATGCAGCAGATCTTGTTACTAAAAATGTTGGACGGCTAAGAGAAATGAGCCCACTTTTGGAAATGGTACAA
GAGGGAATAGATTTAAGCACCGTAAAATGGGATTCTCATTGA

Upstream 100 bases:

>100_bases
GGAGAGAGCGCTGACAACTTTCGTTTAGGCAAGATTATATCTTGAAAAAATTAAGCTTTAGTGCTACTAAATATTGTAAC
TTCTATGCTGAAACCTGACT

Downstream 100 bases:

>100_bases
AGAATAAGACGTTAACCATTAGCAGAGTGATGGTTGTCTAAGCTCTTTCTATGGGTGAAATCGGAAACTTAGCTTTAAGT
TCTTTGTAGATGCCACGGAA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MNMEKIRNAKIKLPIFLDYQSTTKVDPRVLEVMIPYFGEFSNAHSRSHSFGWTAEEAVEKARKHIADLVNADSKEIVFTS
GATESNNMAIKGVAHFYKNKGNHIITVCTEHKCVLDSCRHLENEGFKVTYLSVKQNGIIDLLKLEEAITDKTILISVMMA
NNEIGVMQPVKEIGAMCRKHNIFFHTDAAQSFGKVPIDVNEMNIDLMSLSSHKIYGPMGIGALYVRRKNPRVRLTPLISG
GGQERGMRSGTVPTPLAVGFGEAARIAKEEMEIEASKLEELRDILYNKIKEAFPDVVLNGDYENRIPGNLNLSFPYVEGE
SLIMAIKDLAVSSGSACTSASLEPSYVIRSLNNGHDLEHSSIRFGLGRFTTKDEVLYAADLVTKNVGRLREMSPLLEMVQ
EGIDLSTVKWDSH

Sequences:

>Translated_413_residues
MNMEKIRNAKIKLPIFLDYQSTTKVDPRVLEVMIPYFGEFSNAHSRSHSFGWTAEEAVEKARKHIADLVNADSKEIVFTS
GATESNNMAIKGVAHFYKNKGNHIITVCTEHKCVLDSCRHLENEGFKVTYLSVKQNGIIDLLKLEEAITDKTILISVMMA
NNEIGVMQPVKEIGAMCRKHNIFFHTDAAQSFGKVPIDVNEMNIDLMSLSSHKIYGPMGIGALYVRRKNPRVRLTPLISG
GGQERGMRSGTVPTPLAVGFGEAARIAKEEMEIEASKLEELRDILYNKIKEAFPDVVLNGDYENRIPGNLNLSFPYVEGE
SLIMAIKDLAVSSGSACTSASLEPSYVIRSLNNGHDLEHSSIRFGLGRFTTKDEVLYAADLVTKNVGRLREMSPLLEMVQ
EGIDLSTVKWDSH
>Mature_413_residues
MNMEKIRNAKIKLPIFLDYQSTTKVDPRVLEVMIPYFGEFSNAHSRSHSFGWTAEEAVEKARKHIADLVNADSKEIVFTS
GATESNNMAIKGVAHFYKNKGNHIITVCTEHKCVLDSCRHLENEGFKVTYLSVKQNGIIDLLKLEEAITDKTILISVMMA
NNEIGVMQPVKEIGAMCRKHNIFFHTDAAQSFGKVPIDVNEMNIDLMSLSSHKIYGPMGIGALYVRRKNPRVRLTPLISG
GGQERGMRSGTVPTPLAVGFGEAARIAKEEMEIEASKLEELRDILYNKIKEAFPDVVLNGDYENRIPGNLNLSFPYVEGE
SLIMAIKDLAVSSGSACTSASLEPSYVIRSLNNGHDLEHSSIRFGLGRFTTKDEVLYAADLVTKNVGRLREMSPLLEMVQ
EGIDLSTVKWDSH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=401, Percent_Identity=60.8478802992519, Blast_Score=536, Evalue=1e-152,
Organism=Homo sapiens, GI156713448, Length=408, Percent_Identity=32.5980392156863, Blast_Score=205, Evalue=6e-53,
Organism=Escherichia coli, GI48994898, Length=406, Percent_Identity=62.5615763546798, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1787970, Length=234, Percent_Identity=27.3504273504274, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789175, Length=230, Percent_Identity=28.2608695652174, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=402, Percent_Identity=61.9402985074627, Blast_Score=541, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=33.2317073170732, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=60.0997506234414, Blast_Score=506, Evalue=1e-144,
Organism=Drosophila melanogaster, GI20129463, Length=401, Percent_Identity=64.8379052369077, Blast_Score=551, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45952; Mature: 45952

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMEKIRNAKIKLPIFLDYQSTTKVDPRVLEVMIPYFGEFSNAHSRSHSFGWTAEEAVEK
CCHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
ARKHIADLVNADSKEIVFTSGATESNNMAIKGVAHFYKNKGNHIITVCTEHKCVLDSCRH
HHHHHHHHHCCCCCEEEEECCCCCCCCEEEHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
LENEGFKVTYLSVKQNGIIDLLKLEEAITDKTILISVMMANNEIGVMQPVKEIGAMCRKH
HCCCCEEEEEEEEECCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHC
NIFFHTDAAQSFGKVPIDVNEMNIDLMSLSSHKIYGPMGIGALYVRRKNPRVRLTPLISG
CEEEEEHHHHHHCCCCEEECCCEEEEEECCCCCEECCCCCCEEEEEECCCCEEEEEEECC
GGQERGMRSGTVPTPLAVGFGEAARIAKEEMEIEASKLEELRDILYNKIKEAFPDVVLNG
CCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
DYENRIPGNLNLSFPYVEGESLIMAIKDLAVSSGSACTSASLEPSYVIRSLNNGHDLEHS
CCCCCCCCCEEEEECEECCCHHEEEEHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
SIRFGLGRFTTKDEVLYAADLVTKNVGRLREMSPLLEMVQEGIDLSTVKWDSH
CEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MNMEKIRNAKIKLPIFLDYQSTTKVDPRVLEVMIPYFGEFSNAHSRSHSFGWTAEEAVEK
CCHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
ARKHIADLVNADSKEIVFTSGATESNNMAIKGVAHFYKNKGNHIITVCTEHKCVLDSCRH
HHHHHHHHHCCCCCEEEEECCCCCCCCEEEHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
LENEGFKVTYLSVKQNGIIDLLKLEEAITDKTILISVMMANNEIGVMQPVKEIGAMCRKH
HCCCCEEEEEEEEECCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHC
NIFFHTDAAQSFGKVPIDVNEMNIDLMSLSSHKIYGPMGIGALYVRRKNPRVRLTPLISG
CEEEEEHHHHHHCCCCEEECCCEEEEEECCCCCEECCCCCCEEEEEECCCCEEEEEEECC
GGQERGMRSGTVPTPLAVGFGEAARIAKEEMEIEASKLEELRDILYNKIKEAFPDVVLNG
CCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
DYENRIPGNLNLSFPYVEGESLIMAIKDLAVSSGSACTSASLEPSYVIRSLNNGHDLEHS
CCCCCCCCCEEEEECEECCCHHEEEEHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
SIRFGLGRFTTKDEVLYAADLVTKNVGRLREMSPLLEMVQEGIDLSTVKWDSH
CEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA