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The map label for this gene is thrS [H]

Identifier: 190571398

GI number: 190571398

Start: 1072790

End: 1074691

Strand: Reverse

Name: thrS [H]

Synonym: WPa_1006

Alternate gene names: 190571398

Gene position: 1074691-1072790 (Counterclockwise)

Preceding gene: 190571399

Following gene: 190571397

Centisome position: 72.49

GC content: 37.22

Gene sequence:

>1902_bases
ATGATAAAAGTTACCTTTTTAGCCGAACAAAAAGTAAAAGAATACAGTGGTAGAGTCACTGGCTTTGATATATTACAACC
GGATGCTTTGAGAGAAGCAATTGCCTTTAAGGTAAATGGTGAGTTGTATGATCTCTCACGTGAAATTGAATCTGATACAG
AGATAGAGGTAATACAACTGAGTGACGAAGCGGGTTTAGATATAATAAGGCATGATGCTGCTCATATAATGGCGCAGGCA
GTAAAAGAGCTCTTTCCTAATACTCAGATTACTATCGGGCCAACAATTCAAGATGGTTTCTACTATGATTTTGCTACAGA
TCGTACCTTTACCACGGACGATCTTGCTGCAATAGAAAAAAAAATGAAGGAAATTATAAAAAGTAACCACAGATTCGTCC
GAGAAGTTTGGACTCGTAAGCAGGCAATTGATTTCTTTAGTGATATAGGTGAAAGATACAAGGTTGATATTGTCTCATCA
ATACCAGAAAACGAAAACCTAACTGTTTATAAACAAGGCAACTTTGTAGACCTATGTCGTGGTCCGCACTCACCATCAAC
TGGCAGAGTTAAAGCGTTTAAACTTATGAAAGTGGCAGGTGCATACTGGCGTGGCGATTCTAAGGGCCCAATGTTGCAAA
GAATATATGGCACCGCGTGGAGAAATAAGGATGAATTAAATACTTATCTTAAACGTCTGGAGGAAGCAGAAAAACGCGAC
CACCGCAAAATTGCTAAGGATATGGACTTATTTCACATTCAAGAGGAAGCTGTTGGGCAGATTTTTTGGCATGAACAGGG
ATATACTTTATATAATGTTCTTGAGTCTTACATCAGAAAAAAGTTAATGAACAATGGCTACTTTGAGGTGAAAACCCCTA
TTTTAGTAAGCAAAGAGCTGTGGGAAAAATCGGGACATTGGGATAAGTTTCGTGAAAATATGTTCATTATTGATGAATCT
GAAAGTAAAAAGCTAGCAATAAAACCCATGAATTGCCCTTGTCATGTGCAGATTTTTAATTCTCACATCAGAAGCTATCG
TGATTTACCGATACGTATGGCAGAGTTTGGCACATGCCATAGAAATGAAAGCTCAGGCTCATTGCATGGACTGATGCGAG
TGCGTGGTTTTACGCAAGACGATGCACATATTTTTTGTATGGAAAAACAAGTGAATTCTGAAACTGTAAAGTTTTGTGAC
CTTTTAAAAGAAGTATATTCAGAACTTGGGTTCAATGAAATTTCTGTGAAATTTTCAGACCGTCCAGATACTAGATCAGG
TAATGATGAAGTGTGGGATAGAGCTGAAAGAGCACTGCTTGAAGCAGTTAAAGAAGCAGGGCTTAGTTATGAACTTAATC
CTGGTGAAGGTGCATTTTATGGTCCAAAGTTAGAGTTTATTTTGAAAGATGCAATAGGTAGAAATTGGCAATGTGGAACG
TTGCAAGTTGATTTTATTTTACCGGAGCGGCTTGGAGCTTTTTATATAGGAACAGACGGGCACAAGCATCACCCTGTCAT
GTTACATAGGGCAATTCTTGGAACTTTTGAGCGTTTTATTGGAATTTTAATAGAAAATTATGCAGGAAAATTTCCAGTTT
GGCTTGCTCCAACACAGCTTGCTATTCTGACCATTACAAATGAGTCTGACGGTTATGCTACAGAAATCAGCAACGTTTTA
AAAGAACAAGGTGTGAGAATCAAAACTGATTTGACCAATGAAAAAATTAGCTATAAGATACGTTTGCATAGTTCAAGTAA
GGTACCTATATTATGGATCATAGGCAAAAATGAAGTTACAAATAAAACTGTGTCAGTGAGAAATTTAGGATCGGAAAAAC
AGGAGTCTTTTTCTTTGGAGAATGCTACTGAATTGCTGTTAAAGATTATTAATTTGAATTAA

Upstream 100 bases:

>100_bases
CTCACCTTTTGGTGTCATTCCAGCGCGTGGCGCTGGAATCTAGAAAAGAAAGAAACATGGATCCCAGTGTCAAGCACTGG
GATGATAAAAGGTCTAATGA

Downstream 100 bases:

>100_bases
TGGAGCTAAAATTTGCAAGTCAAAAAGAACAATAAAAACAGAATTAATGAACTCATCACAGCTAAGGAGGTACGCTTAGT
TGATCATAGTGGTGAAATGG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MIKVTFLAEQKVKEYSGRVTGFDILQPDALREAIAFKVNGELYDLSREIESDTEIEVIQLSDEAGLDIIRHDAAHIMAQA
VKELFPNTQITIGPTIQDGFYYDFATDRTFTTDDLAAIEKKMKEIIKSNHRFVREVWTRKQAIDFFSDIGERYKVDIVSS
IPENENLTVYKQGNFVDLCRGPHSPSTGRVKAFKLMKVAGAYWRGDSKGPMLQRIYGTAWRNKDELNTYLKRLEEAEKRD
HRKIAKDMDLFHIQEEAVGQIFWHEQGYTLYNVLESYIRKKLMNNGYFEVKTPILVSKELWEKSGHWDKFRENMFIIDES
ESKKLAIKPMNCPCHVQIFNSHIRSYRDLPIRMAEFGTCHRNESSGSLHGLMRVRGFTQDDAHIFCMEKQVNSETVKFCD
LLKEVYSELGFNEISVKFSDRPDTRSGNDEVWDRAERALLEAVKEAGLSYELNPGEGAFYGPKLEFILKDAIGRNWQCGT
LQVDFILPERLGAFYIGTDGHKHHPVMLHRAILGTFERFIGILIENYAGKFPVWLAPTQLAILTITNESDGYATEISNVL
KEQGVRIKTDLTNEKISYKIRLHSSSKVPILWIIGKNEVTNKTVSVRNLGSEKQESFSLENATELLLKIINLN

Sequences:

>Translated_633_residues
MIKVTFLAEQKVKEYSGRVTGFDILQPDALREAIAFKVNGELYDLSREIESDTEIEVIQLSDEAGLDIIRHDAAHIMAQA
VKELFPNTQITIGPTIQDGFYYDFATDRTFTTDDLAAIEKKMKEIIKSNHRFVREVWTRKQAIDFFSDIGERYKVDIVSS
IPENENLTVYKQGNFVDLCRGPHSPSTGRVKAFKLMKVAGAYWRGDSKGPMLQRIYGTAWRNKDELNTYLKRLEEAEKRD
HRKIAKDMDLFHIQEEAVGQIFWHEQGYTLYNVLESYIRKKLMNNGYFEVKTPILVSKELWEKSGHWDKFRENMFIIDES
ESKKLAIKPMNCPCHVQIFNSHIRSYRDLPIRMAEFGTCHRNESSGSLHGLMRVRGFTQDDAHIFCMEKQVNSETVKFCD
LLKEVYSELGFNEISVKFSDRPDTRSGNDEVWDRAERALLEAVKEAGLSYELNPGEGAFYGPKLEFILKDAIGRNWQCGT
LQVDFILPERLGAFYIGTDGHKHHPVMLHRAILGTFERFIGILIENYAGKFPVWLAPTQLAILTITNESDGYATEISNVL
KEQGVRIKTDLTNEKISYKIRLHSSSKVPILWIIGKNEVTNKTVSVRNLGSEKQESFSLENATELLLKIINLN
>Mature_633_residues
MIKVTFLAEQKVKEYSGRVTGFDILQPDALREAIAFKVNGELYDLSREIESDTEIEVIQLSDEAGLDIIRHDAAHIMAQA
VKELFPNTQITIGPTIQDGFYYDFATDRTFTTDDLAAIEKKMKEIIKSNHRFVREVWTRKQAIDFFSDIGERYKVDIVSS
IPENENLTVYKQGNFVDLCRGPHSPSTGRVKAFKLMKVAGAYWRGDSKGPMLQRIYGTAWRNKDELNTYLKRLEEAEKRD
HRKIAKDMDLFHIQEEAVGQIFWHEQGYTLYNVLESYIRKKLMNNGYFEVKTPILVSKELWEKSGHWDKFRENMFIIDES
ESKKLAIKPMNCPCHVQIFNSHIRSYRDLPIRMAEFGTCHRNESSGSLHGLMRVRGFTQDDAHIFCMEKQVNSETVKFCD
LLKEVYSELGFNEISVKFSDRPDTRSGNDEVWDRAERALLEAVKEAGLSYELNPGEGAFYGPKLEFILKDAIGRNWQCGT
LQVDFILPERLGAFYIGTDGHKHHPVMLHRAILGTFERFIGILIENYAGKFPVWLAPTQLAILTITNESDGYATEISNVL
KEQGVRIKTDLTNEKISYKIRLHSSSKVPILWIIGKNEVTNKTVSVRNLGSEKQESFSLENATELLLKIINLN

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=574, Percent_Identity=39.7212543554007, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI38202255, Length=612, Percent_Identity=38.562091503268, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI20070344, Length=652, Percent_Identity=35.2760736196319, Blast_Score=425, Evalue=1e-119,
Organism=Escherichia coli, GI1788013, Length=633, Percent_Identity=49.6050552922591, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=166, Percent_Identity=24.0963855421687, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71984192, Length=613, Percent_Identity=38.4991843393148, Blast_Score=456, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71984184, Length=613, Percent_Identity=38.4991843393148, Blast_Score=456, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322112, Length=619, Percent_Identity=39.0953150242326, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6322654, Length=418, Percent_Identity=33.9712918660287, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24583839, Length=633, Percent_Identity=38.3886255924171, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24583842, Length=633, Percent_Identity=38.3886255924171, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI28574141, Length=633, Percent_Identity=38.3886255924171, Blast_Score=466, Evalue=1e-131,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 72757; Mature: 72757

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVTFLAEQKVKEYSGRVTGFDILQPDALREAIAFKVNGELYDLSREIESDTEIEVIQL
CEEEEEEHHHHHHHHCCCCCEEECCCCHHHHHHEEEEECCCEEEHHHHCCCCCCEEEEEE
SDEAGLDIIRHDAAHIMAQAVKELFPNTQITIGPTIQDGFYYDFATDRTFTTDDLAAIEK
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCEEEEECCCCCCCHHHHHHHHH
KMKEIIKSNHRFVREVWTRKQAIDFFSDIGERYKVDIVSSIPENENLTVYKQGNFVDLCR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHCCCCCCCCEEEEECCCEEEECC
GPHSPSTGRVKAFKLMKVAGAYWRGDSKGPMLQRIYGTAWRNKDELNTYLKRLEEAEKRD
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HRKIAKDMDLFHIQEEAVGQIFWHEQGYTLYNVLESYIRKKLMNNGYFEVKTPILVSKEL
HHHHHHHHHHEEECHHHHCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCEEECHHH
WEKSGHWDKFRENMFIIDESESKKLAIKPMNCPCHVQIFNSHIRSYRDLPIRMAEFGTCH
HHCCCCHHHHHCCEEEEECCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEHHHCCCCC
RNESSGSLHGLMRVRGFTQDDAHIFCMEKQVNSETVKFCDLLKEVYSELGFNEISVKFSD
CCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
RPDTRSGNDEVWDRAERALLEAVKEAGLSYELNPGEGAFYGPKLEFILKDAIGRNWQCGT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECCHHHHHHHHHCCCCCCCCE
LQVDFILPERLGAFYIGTDGHKHHPVMLHRAILGTFERFIGILIENYAGKFPVWLAPTQL
EEEEEEEHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEE
AILTITNESDGYATEISNVLKEQGVRIKTDLTNEKISYKIRLHSSSKVPILWIIGKNEVT
EEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCCEEEEECCCCCC
NKTVSVRNLGSEKQESFSLENATELLLKIINLN
CCEEEEHHCCCCHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKVTFLAEQKVKEYSGRVTGFDILQPDALREAIAFKVNGELYDLSREIESDTEIEVIQL
CEEEEEEHHHHHHHHCCCCCEEECCCCHHHHHHEEEEECCCEEEHHHHCCCCCCEEEEEE
SDEAGLDIIRHDAAHIMAQAVKELFPNTQITIGPTIQDGFYYDFATDRTFTTDDLAAIEK
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCEEEEECCCCCCCHHHHHHHHH
KMKEIIKSNHRFVREVWTRKQAIDFFSDIGERYKVDIVSSIPENENLTVYKQGNFVDLCR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHCCCCCCCCEEEEECCCEEEECC
GPHSPSTGRVKAFKLMKVAGAYWRGDSKGPMLQRIYGTAWRNKDELNTYLKRLEEAEKRD
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HRKIAKDMDLFHIQEEAVGQIFWHEQGYTLYNVLESYIRKKLMNNGYFEVKTPILVSKEL
HHHHHHHHHHEEECHHHHCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCEEECHHH
WEKSGHWDKFRENMFIIDESESKKLAIKPMNCPCHVQIFNSHIRSYRDLPIRMAEFGTCH
HHCCCCHHHHHCCEEEEECCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCEEHHHCCCCC
RNESSGSLHGLMRVRGFTQDDAHIFCMEKQVNSETVKFCDLLKEVYSELGFNEISVKFSD
CCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
RPDTRSGNDEVWDRAERALLEAVKEAGLSYELNPGEGAFYGPKLEFILKDAIGRNWQCGT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECCHHHHHHHHHCCCCCCCCE
LQVDFILPERLGAFYIGTDGHKHHPVMLHRAILGTFERFIGILIENYAGKFPVWLAPTQL
EEEEEEEHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEE
AILTITNESDGYATEISNVLKEQGVRIKTDLTNEKISYKIRLHSSSKVPILWIIGKNEVT
EEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCCEEEEECCCCCC
NKTVSVRNLGSEKQESFSLENATELLLKIINLN
CCEEEEHHCCCCHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA