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The map label for this gene is purB [H]

Identifier: 190571060

GI number: 190571060

Start: 718699

End: 720000

Strand: Reverse

Name: purB [H]

Synonym: WPa_0654

Alternate gene names: 190571060

Gene position: 720000-718699 (Counterclockwise)

Preceding gene: 190571061

Following gene: 190571059

Centisome position: 48.57

GC content: 34.49

Gene sequence:

>1302_bases
ATGATTCCACGCTATAGCCGCAAAGAAATCTCTTCTATTTGGGAAGAAAAAAATAAGTTCAACATATGGCTCAAAATAGA
AAAATTAGCATGTGAAGCTCAAGCAAAATTAAAAGTTATTCCAAATGATGTTGCTGAAAAGCTCTCTGGTGCTATTGAAT
TTGATATTGAGCGTATTAACGAAATTGAATCCATTGTAAAACATGATGTTATAGCCTTTTTGACATATATTGCTGAAAAA
GCGGGAGTTGATGTTCGTTATCTCCATTACGGAATGACAAGTTCTGATGTTTTAGATACATGCTTTGCAGTGCAGTTGAA
AGAGTCATGTGATATTTTACTCAAGAATCTAAAAAATTTACTTATAGTATTGAAAAAAAAAGCTGAGGACTATAAAGATA
TTGTTTGTGTTGGGCGCAGTCATGGAATGCATGCAGAACCAATAACTCTTGGATTAAAATTTGCTAGATTTTATGCTGAA
TTTAAACGCAATTATCAGAGATTAATTAGCGCGCAAAAAGAGATCTCAATTTGTAAAATATCAGGTGCAGTAGGTAATTT
TACAAATATTAATCCATTTGTTGAAGAGTATGTAGCGAAAGAAATGGGACTCATACCTGAAACCATATCGTCTCAAGTCA
TCCCTCGTGATAGACATGCCATGTTCTTTTCAATTTTGGGAGTAATTGCAAGCTCAATAGAAAATATTGCAATTGAGATT
CGTCATTTGCAAAGAACTGAAGTCGGCGAAATCTCTGAATATTTTTCCACTGGACAGAAGGGAAGTTCTGCTATGCCGCA
TAAGTGTAATCCTATTTTAAGTGAGAATTTGACTGGGCTCTCACGCTTAGTACGCAGCTACGTTTTTCCTGCATTAGAAA
ATGTTGCATTATGGCACGAACGAGATATATCGCACTCGTCTGTGGAAAGGTGCATTGCTCCTGATGCTTGTATAGCAATG
GATTTTGCTTTAGTACGATTAACAGATTTGATAGATAAATTGGTGATCAATAAGGAAAATATTGAAAAGAACTTAAATTC
TTCAAAAGGCTTGGTTTTTTCACAACGAGTATTACTCGAGTTGGTAAATAGTGGTTTGGCGAGGGAAGAGGCGTATAAAA
TTGTTCAGAGCAATGCAATGAAAGTGAAACAAAACAATAGTGATTTTCTGGCCGAACTGAAACAAGATAAATCCTTACTT
GAAGTTATTAACTCTAAAAAACTTGAATCTTTGTTTGATTTGAAGTATTATACGAAGCATGTAGATCATATATATAGCAA
GGTTTTTAATGAGGCTGAGTAG

Upstream 100 bases:

>100_bases
GAAATGTCTTCCATTGCTAGGCTGGTATCATCCCAGTGCTCCCCTCATGTCATTCCAGTGCATGACACTGGAATGACACC
ATTATTGGAATAAATCAAAA

Downstream 100 bases:

>100_bases
CTCAGTGGTAGAGCAGGAGGATCATAATCTCTTGGTCGGGGGTTCAAATCCCTCCTCAGCCACTCAAGCTAAGCTATGGT
TTGGCGATAGCAGCGCATTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIPRYSRKEISSIWEEKNKFNIWLKIEKLACEAQAKLKVIPNDVAEKLSGAIEFDIERINEIESIVKHDVIAFLTYIAEK
AGVDVRYLHYGMTSSDVLDTCFAVQLKESCDILLKNLKNLLIVLKKKAEDYKDIVCVGRSHGMHAEPITLGLKFARFYAE
FKRNYQRLISAQKEISICKISGAVGNFTNINPFVEEYVAKEMGLIPETISSQVIPRDRHAMFFSILGVIASSIENIAIEI
RHLQRTEVGEISEYFSTGQKGSSAMPHKCNPILSENLTGLSRLVRSYVFPALENVALWHERDISHSSVERCIAPDACIAM
DFALVRLTDLIDKLVINKENIEKNLNSSKGLVFSQRVLLELVNSGLAREEAYKIVQSNAMKVKQNNSDFLAELKQDKSLL
EVINSKKLESLFDLKYYTKHVDHIYSKVFNEAE

Sequences:

>Translated_433_residues
MIPRYSRKEISSIWEEKNKFNIWLKIEKLACEAQAKLKVIPNDVAEKLSGAIEFDIERINEIESIVKHDVIAFLTYIAEK
AGVDVRYLHYGMTSSDVLDTCFAVQLKESCDILLKNLKNLLIVLKKKAEDYKDIVCVGRSHGMHAEPITLGLKFARFYAE
FKRNYQRLISAQKEISICKISGAVGNFTNINPFVEEYVAKEMGLIPETISSQVIPRDRHAMFFSILGVIASSIENIAIEI
RHLQRTEVGEISEYFSTGQKGSSAMPHKCNPILSENLTGLSRLVRSYVFPALENVALWHERDISHSSVERCIAPDACIAM
DFALVRLTDLIDKLVINKENIEKNLNSSKGLVFSQRVLLELVNSGLAREEAYKIVQSNAMKVKQNNSDFLAELKQDKSLL
EVINSKKLESLFDLKYYTKHVDHIYSKVFNEAE
>Mature_433_residues
MIPRYSRKEISSIWEEKNKFNIWLKIEKLACEAQAKLKVIPNDVAEKLSGAIEFDIERINEIESIVKHDVIAFLTYIAEK
AGVDVRYLHYGMTSSDVLDTCFAVQLKESCDILLKNLKNLLIVLKKKAEDYKDIVCVGRSHGMHAEPITLGLKFARFYAE
FKRNYQRLISAQKEISICKISGAVGNFTNINPFVEEYVAKEMGLIPETISSQVIPRDRHAMFFSILGVIASSIENIAIEI
RHLQRTEVGEISEYFSTGQKGSSAMPHKCNPILSENLTGLSRLVRSYVFPALENVALWHERDISHSSVERCIAPDACIAM
DFALVRLTDLIDKLVINKENIEKNLNSSKGLVFSQRVLLELVNSGLAREEAYKIVQSNAMKVKQNNSDFLAELKQDKSLL
EVINSKKLESLFDLKYYTKHVDHIYSKVFNEAE

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=450, Percent_Identity=23.3333333333333, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI183227688, Length=406, Percent_Identity=23.6453201970443, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1787376, Length=360, Percent_Identity=29.7222222222222, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787896, Length=201, Percent_Identity=30.3482587064677, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=401, Percent_Identity=26.6832917705736, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI32564234, Length=361, Percent_Identity=27.7008310249307, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17553882, Length=304, Percent_Identity=25.3289473684211, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI32565146, Length=298, Percent_Identity=25.8389261744966, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323391, Length=422, Percent_Identity=24.8815165876777, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6324993, Length=175, Percent_Identity=30.8571428571429, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24647570, Length=442, Percent_Identity=23.7556561085973, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24640179, Length=311, Percent_Identity=27.0096463022508, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24640177, Length=217, Percent_Identity=31.7972350230415, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI78710009, Length=283, Percent_Identity=27.5618374558304, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49216; Mature: 49216

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRKEISSIWEEKNKFNIWLKIEKLACEAQAKLKVIPNDVAEKLSGAIEFDIERIN
CCCCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHEEECCHHHHHHHCCCHHHHHHHHH
EIESIVKHDVIAFLTYIAEKAGVDVRYLHYGMTSSDVLDTCFAVQLKESCDILLKNLKNL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LIVLKKKAEDYKDIVCVGRSHGMHAEPITLGLKFARFYAEFKRNYQRLISAQKEISICKI
HHHHHHHHCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
SGAVGNFTNINPFVEEYVAKEMGLIPETISSQVIPRDRHAMFFSILGVIASSIENIAIEI
ECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVGEISEYFSTGQKGSSAMPHKCNPILSENLTGLSRLVRSYVFPALENVALWHE
HHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERCIAPDACIAMDFALVRLTDLIDKLVINKENIEKNLNSSKGLVFSQRVLLE
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHH
LVNSGLAREEAYKIVQSNAMKVKQNNSDFLAELKQDKSLLEVINSKKLESLFDLKYYTKH
HHHCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDHIYSKVFNEAE
HHHHHHHHHCCCC
>Mature Secondary Structure
MIPRYSRKEISSIWEEKNKFNIWLKIEKLACEAQAKLKVIPNDVAEKLSGAIEFDIERIN
CCCCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHEEECCHHHHHHHCCCHHHHHHHHH
EIESIVKHDVIAFLTYIAEKAGVDVRYLHYGMTSSDVLDTCFAVQLKESCDILLKNLKNL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LIVLKKKAEDYKDIVCVGRSHGMHAEPITLGLKFARFYAEFKRNYQRLISAQKEISICKI
HHHHHHHHCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
SGAVGNFTNINPFVEEYVAKEMGLIPETISSQVIPRDRHAMFFSILGVIASSIENIAIEI
ECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVGEISEYFSTGQKGSSAMPHKCNPILSENLTGLSRLVRSYVFPALENVALWHE
HHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERCIAPDACIAMDFALVRLTDLIDKLVINKENIEKNLNSSKGLVFSQRVLLE
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHH
LVNSGLAREEAYKIVQSNAMKVKQNNSDFLAELKQDKSLLEVINSKKLESLFDLKYYTKH
HHHCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDHIYSKVFNEAE
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571; 10673438 [H]