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The map label for this gene is pccA [H]

Identifier: 190571025

GI number: 190571025

Start: 669476

End: 671446

Strand: Direct

Name: pccA [H]

Synonym: WPa_0617

Alternate gene names: 190571025

Gene position: 669476-671446 (Clockwise)

Preceding gene: 190571024

Following gene: 190571032

Centisome position: 45.16

GC content: 36.07

Gene sequence:

>1971_bases
ATGATAAGAAAGAAGTATAGTAAGATTTTAGTAGCGAACAGAGGGGAGATTGCCTGCAGAATAATCAAAACTGCGCACAA
GATGGGCATATCTTGTGTGTCTGTGTACTCAGATGCAGATGCAAATTCTGTGCATGTAAAGCAAGCAGATGAGTCAAGGC
ACATTGGCCCTTCGCCTGCTTATCTTAGTTACTTAAATATCGAAAAAATATGCGAAGTAGCAGTTGAAACAGGTGCCGAG
GCGGTTCATCCTGGCTATGGGTTTTTAGCAGAAAATCCAGATTTTCCCCGTGCTCTGGAAAAACATAACATAGATTTCAT
TGGTCCAAGTGCAGAAACAATAGAAGTTACAGCCAATAAAATAACGGCAAAAGAAGAGGCAAGAAAAGCTGGAGTAAATG
TAGTGCCAGGATATATGGGTAAGATCGAAGATGCTGCCCACGCAGCCATCGTTGCTGAAGAGATTGGTTTTCCCGTTATG
CTCAAAGCTGCATCAGGTGGTGGTGGCAAAGGAATGCGAATTGTATATTCCAAAAAAGAAATTGAACTAGCATTTACATC
AGCAACAAATGAAGCAGAGAAAAGTTTTAAAGATGGCAGTATTTTTATAGAGAAATATATAGAGCTGCCAAGACACATTG
AAATACAAATCATAGCAGATAAATACGGCAATATAGTGTGTCTTGGGGAGAGAGAGTGCTCGGTACAGAGGAATAACCAG
AAAATAATAGAAGAAACACCAAGCCCATTTATTAGTGAAGAAGTAAGACAAAAAATGTATGCTCAATGTGTTTCTTTGGC
AAAGCAAGTTGATTATTTTTCAGCAGGTACTGTCGAGTTTGTTGTAGATAAGAACCAAAACTTCTATTTTCTTGAGGTAA
ATACGAGATTACAAGTTGAGCATCCAGTCACAGAATTTGTCACTGGAATAGATATAGTGGAAGAGATGATCAGAATTTCT
TGCGGAGAAAAATTAAGATTTGGTCAGAATGATATTAAACTTATTGGCTCTGCAATAGAAAGCAGAATTTGCGCTGAAGA
TCCATCAAAGAGATTTTTTCCTTCCAGCGGAAGAATCAAATATTACGACAAACCAGATGATGTAAGAATAGATGATGGAG
TAGCTAGCGGTTCAGAAATCAGCATGTTCTACGACTCAATGATTGCAAAAATTATAACCTATGGAAAAGATAGAATAGAA
GCAATCAGCAGAATGCAAAAAGCATTGTCTGAATGTTATATAGAAGGAGTAACAAATAATATAGAATTTCTAGAATCCAT
TTTCCATCATCCAAACTTTATTGCAGCAAAACTCCACACAAGATTCATTCCAGACCATTATCCTAGTGGGTTTCAAGGCG
ATTTTATCACAGAGGAATATATTAAAATATTTATTTTTGCTGCACTGTATGTTCACTTAGAAAATGAGGGAAAGTATTAC
AGTAAGTCAACAGACGAAGCATTCATAGTGAAAATAGATGACAATGAGTACTCCGTAAATGCAAAGTATCAAGATAACAC
ATTAACAACAGTATATAACCACAATAAATACTCCGTGGTGGGTAAGTGGAAATCAAGTTACAGATTACTATATATCACAA
TTAATGATGATACCAATATAGCACTTAAAATAGAAAGACAAGGTAGCAAATACTTTATAAGACACGCAGGTATGAAAGCT
AAATGTTGTGTATTGAAACCTCATGTAGCTGAACTAAGCAAATTAATGCTAAACAACAAAACAGAAGGGATTTCAGTAGA
CGCTGTAAAATCTCCAATATCCGGTTTATTGGTTAAATTACACGTCAATGCTGGAGATCAGGTAGAGGTAGGACAACCTC
TCTTTGTTGTGGAGGCAATGAAAATGGAAAATATAATATGTGCTGAATCAGAAATGGTGATAAAAAATATCCCTGTTAAA
GAAGGAAAAAATATACATGCTGGTGATTTGGTATTAGATCTGATTTCTTAA

Upstream 100 bases:

>100_bases
GTTATGCAACTTTTAGATAGTGGTTTAGCCCCAGTGTGAAGTATTTGCAGGTAGTAATATAGAAATTTACTTTCTTAACT
TAAGAAAGTATACTTATAAG

Downstream 100 bases:

>100_bases
ATAACGCAAGCAGTATAAACCTGATCCAAATAAGATAAATGAAAACATTTGATGGGCTATAGCAATAGCCATTGGTATAT
GCAGCAACAAAGTGACTATT

Product: propionyl-CoA carboxylase, alpha subunit

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MIRKKYSKILVANRGEIACRIIKTAHKMGISCVSVYSDADANSVHVKQADESRHIGPSPAYLSYLNIEKICEVAVETGAE
AVHPGYGFLAENPDFPRALEKHNIDFIGPSAETIEVTANKITAKEEARKAGVNVVPGYMGKIEDAAHAAIVAEEIGFPVM
LKAASGGGGKGMRIVYSKKEIELAFTSATNEAEKSFKDGSIFIEKYIELPRHIEIQIIADKYGNIVCLGERECSVQRNNQ
KIIEETPSPFISEEVRQKMYAQCVSLAKQVDYFSAGTVEFVVDKNQNFYFLEVNTRLQVEHPVTEFVTGIDIVEEMIRIS
CGEKLRFGQNDIKLIGSAIESRICAEDPSKRFFPSSGRIKYYDKPDDVRIDDGVASGSEISMFYDSMIAKIITYGKDRIE
AISRMQKALSECYIEGVTNNIEFLESIFHHPNFIAAKLHTRFIPDHYPSGFQGDFITEEYIKIFIFAALYVHLENEGKYY
SKSTDEAFIVKIDDNEYSVNAKYQDNTLTTVYNHNKYSVVGKWKSSYRLLYITINDDTNIALKIERQGSKYFIRHAGMKA
KCCVLKPHVAELSKLMLNNKTEGISVDAVKSPISGLLVKLHVNAGDQVEVGQPLFVVEAMKMENIICAESEMVIKNIPVK
EGKNIHAGDLVLDLIS

Sequences:

>Translated_656_residues
MIRKKYSKILVANRGEIACRIIKTAHKMGISCVSVYSDADANSVHVKQADESRHIGPSPAYLSYLNIEKICEVAVETGAE
AVHPGYGFLAENPDFPRALEKHNIDFIGPSAETIEVTANKITAKEEARKAGVNVVPGYMGKIEDAAHAAIVAEEIGFPVM
LKAASGGGGKGMRIVYSKKEIELAFTSATNEAEKSFKDGSIFIEKYIELPRHIEIQIIADKYGNIVCLGERECSVQRNNQ
KIIEETPSPFISEEVRQKMYAQCVSLAKQVDYFSAGTVEFVVDKNQNFYFLEVNTRLQVEHPVTEFVTGIDIVEEMIRIS
CGEKLRFGQNDIKLIGSAIESRICAEDPSKRFFPSSGRIKYYDKPDDVRIDDGVASGSEISMFYDSMIAKIITYGKDRIE
AISRMQKALSECYIEGVTNNIEFLESIFHHPNFIAAKLHTRFIPDHYPSGFQGDFITEEYIKIFIFAALYVHLENEGKYY
SKSTDEAFIVKIDDNEYSVNAKYQDNTLTTVYNHNKYSVVGKWKSSYRLLYITINDDTNIALKIERQGSKYFIRHAGMKA
KCCVLKPHVAELSKLMLNNKTEGISVDAVKSPISGLLVKLHVNAGDQVEVGQPLFVVEAMKMENIICAESEMVIKNIPVK
EGKNIHAGDLVLDLIS
>Mature_656_residues
MIRKKYSKILVANRGEIACRIIKTAHKMGISCVSVYSDADANSVHVKQADESRHIGPSPAYLSYLNIEKICEVAVETGAE
AVHPGYGFLAENPDFPRALEKHNIDFIGPSAETIEVTANKITAKEEARKAGVNVVPGYMGKIEDAAHAAIVAEEIGFPVM
LKAASGGGGKGMRIVYSKKEIELAFTSATNEAEKSFKDGSIFIEKYIELPRHIEIQIIADKYGNIVCLGERECSVQRNNQ
KIIEETPSPFISEEVRQKMYAQCVSLAKQVDYFSAGTVEFVVDKNQNFYFLEVNTRLQVEHPVTEFVTGIDIVEEMIRIS
CGEKLRFGQNDIKLIGSAIESRICAEDPSKRFFPSSGRIKYYDKPDDVRIDDGVASGSEISMFYDSMIAKIITYGKDRIE
AISRMQKALSECYIEGVTNNIEFLESIFHHPNFIAAKLHTRFIPDHYPSGFQGDFITEEYIKIFIFAALYVHLENEGKYY
SKSTDEAFIVKIDDNEYSVNAKYQDNTLTTVYNHNKYSVVGKWKSSYRLLYITINDDTNIALKIERQGSKYFIRHAGMKA
KCCVLKPHVAELSKLMLNNKTEGISVDAVKSPISGLLVKLHVNAGDQVEVGQPLFVVEAMKMENIICAESEMVIKNIPVK
EGKNIHAGDLVLDLIS

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG4770

COG function: function code I; Acetyl/propionyl-CoA carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI189095269, Length=669, Percent_Identity=44.2451420029895, Blast_Score=559, Evalue=1e-159,
Organism=Homo sapiens, GI65506442, Length=669, Percent_Identity=44.2451420029895, Blast_Score=558, Evalue=1e-159,
Organism=Homo sapiens, GI295821183, Length=668, Percent_Identity=42.2155688622754, Blast_Score=512, Evalue=1e-145,
Organism=Homo sapiens, GI116805327, Length=671, Percent_Identity=40.2384500745156, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI106049528, Length=456, Percent_Identity=43.2017543859649, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI106049295, Length=456, Percent_Identity=43.2017543859649, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI106049292, Length=456, Percent_Identity=43.2017543859649, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI38679974, Length=502, Percent_Identity=31.4741035856574, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI38679960, Length=502, Percent_Identity=31.2749003984064, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI38679971, Length=502, Percent_Identity=31.4741035856574, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI38679977, Length=502, Percent_Identity=31.4741035856574, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI38679967, Length=502, Percent_Identity=31.4741035856574, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI134142062, Length=500, Percent_Identity=30.8, Blast_Score=225, Evalue=8e-59,
Organism=Escherichia coli, GI1789654, Length=429, Percent_Identity=44.2890442890443, Blast_Score=357, Evalue=2e-99,
Organism=Escherichia coli, GI1786216, Length=279, Percent_Identity=21.8637992831541, Blast_Score=62, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17567343, Length=677, Percent_Identity=41.2112259970458, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71987519, Length=650, Percent_Identity=40.6153846153846, Blast_Score=477, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17562816, Length=456, Percent_Identity=44.2982456140351, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71997163, Length=740, Percent_Identity=27.972972972973, Blast_Score=242, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI71997168, Length=706, Percent_Identity=28.1869688385269, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI133931226, Length=704, Percent_Identity=27.9829545454545, Blast_Score=233, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=439, Percent_Identity=45.3302961275626, Blast_Score=382, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6319695, Length=455, Percent_Identity=42.8571428571429, Blast_Score=332, Evalue=8e-92,
Organism=Saccharomyces cerevisiae, GI6321376, Length=455, Percent_Identity=43.7362637362637, Blast_Score=327, Evalue=4e-90,
Organism=Saccharomyces cerevisiae, GI6323863, Length=675, Percent_Identity=28.8888888888889, Blast_Score=234, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6324343, Length=736, Percent_Identity=27.7173913043478, Blast_Score=219, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24651757, Length=674, Percent_Identity=37.6854599406528, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651759, Length=634, Percent_Identity=36.4353312302839, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24652212, Length=456, Percent_Identity=43.421052631579, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24652210, Length=456, Percent_Identity=43.421052631579, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24652214, Length=456, Percent_Identity=43.421052631579, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI19921944, Length=456, Percent_Identity=43.421052631579, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24652216, Length=456, Percent_Identity=43.421052631579, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI281363050, Length=456, Percent_Identity=43.421052631579, Blast_Score=355, Evalue=7e-98,
Organism=Drosophila melanogaster, GI24652224, Length=456, Percent_Identity=43.421052631579, Blast_Score=355, Evalue=7e-98,
Organism=Drosophila melanogaster, GI24652222, Length=456, Percent_Identity=43.421052631579, Blast_Score=355, Evalue=7e-98,
Organism=Drosophila melanogaster, GI24652220, Length=456, Percent_Identity=43.421052631579, Blast_Score=355, Evalue=7e-98,
Organism=Drosophila melanogaster, GI24652218, Length=456, Percent_Identity=43.421052631579, Blast_Score=355, Evalue=7e-98,
Organism=Drosophila melanogaster, GI161076409, Length=498, Percent_Identity=31.1244979919679, Blast_Score=225, Evalue=8e-59,
Organism=Drosophila melanogaster, GI161076407, Length=498, Percent_Identity=31.1244979919679, Blast_Score=225, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24586460, Length=498, Percent_Identity=31.1244979919679, Blast_Score=225, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24586458, Length=498, Percent_Identity=31.1244979919679, Blast_Score=225, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 73390; Mature: 73390

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKKYSKILVANRGEIACRIIKTAHKMGISCVSVYSDADANSVHVKQADESRHIGPSPA
CCCCCCCEEEEECCCCEEEHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCH
YLSYLNIEKICEVAVETGAEAVHPGYGFLAENPDFPRALEKHNIDFIGPSAETIEVTANK
HEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCCCCEEEEEECH
ITAKEEARKAGVNVVPGYMGKIEDAAHAAIVAEEIGFPVMLKAASGGGGKGMRIVYSKKE
HHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCE
IELAFTSATNEAEKSFKDGSIFIEKYIELPRHIEIQIIADKYGNIVCLGERECSVQRNNQ
EEEEEECCCCHHHHCCCCCHHHHHHHHCCCCEEEEEEEEECCCCEEEEECCCCCCCCCCC
KIIEETPSPFISEEVRQKMYAQCVSLAKQVDYFSAGTVEFVVDKNQNFYFLEVNTRLQVE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCEEEEC
HPVTEFVTGIDIVEEMIRISCGEKLRFGQNDIKLIGSAIESRICAEDPSKRFFPSSGRIK
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
YYDKPDDVRIDDGVASGSEISMFYDSMIAKIITYGKDRIEAISRMQKALSECYIEGVTNN
EECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
IEFLESIFHHPNFIAAKLHTRFIPDHYPSGFQGDFITEEYIKIFIFAALYVHLENEGKYY
HHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCEE
SKSTDEAFIVKIDDNEYSVNAKYQDNTLTTVYNHNKYSVVGKWKSSYRLLYITINDDTNI
CCCCCCEEEEEECCCCEEEEEEEECCEEEEEEECCEEEEEEEECCCEEEEEEEECCCCEE
ALKIERQGSKYFIRHAGMKAKCCVLKPHVAELSKLMLNNKTEGISVDAVKSPISGLLVKL
EEEEEECCCEEEEEECCCCCEEEEECCCHHHHHHHHHCCCCCCEEEHHHHCCCCCEEEEE
HVNAGDQVEVGQPLFVVEAMKMENIICAESEMVIKNIPVKEGKNIHAGDLVLDLIS
EECCCCCEECCCCEEEEEHHHHCCEEECCCCEEEECCCCCCCCCCCHHHHHHEECC
>Mature Secondary Structure
MIRKKYSKILVANRGEIACRIIKTAHKMGISCVSVYSDADANSVHVKQADESRHIGPSPA
CCCCCCCEEEEECCCCEEEHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCH
YLSYLNIEKICEVAVETGAEAVHPGYGFLAENPDFPRALEKHNIDFIGPSAETIEVTANK
HEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCCCCEEEEEECH
ITAKEEARKAGVNVVPGYMGKIEDAAHAAIVAEEIGFPVMLKAASGGGGKGMRIVYSKKE
HHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEECCE
IELAFTSATNEAEKSFKDGSIFIEKYIELPRHIEIQIIADKYGNIVCLGERECSVQRNNQ
EEEEEECCCCHHHHCCCCCHHHHHHHHCCCCEEEEEEEEECCCCEEEEECCCCCCCCCCC
KIIEETPSPFISEEVRQKMYAQCVSLAKQVDYFSAGTVEFVVDKNQNFYFLEVNTRLQVE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCEEEEC
HPVTEFVTGIDIVEEMIRISCGEKLRFGQNDIKLIGSAIESRICAEDPSKRFFPSSGRIK
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
YYDKPDDVRIDDGVASGSEISMFYDSMIAKIITYGKDRIEAISRMQKALSECYIEGVTNN
EECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
IEFLESIFHHPNFIAAKLHTRFIPDHYPSGFQGDFITEEYIKIFIFAALYVHLENEGKYY
HHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCEE
SKSTDEAFIVKIDDNEYSVNAKYQDNTLTTVYNHNKYSVVGKWKSSYRLLYITINDDTNI
CCCCCCEEEEEECCCCEEEEEEEECCEEEEEEECCEEEEEEEECCCEEEEEEEECCCCEE
ALKIERQGSKYFIRHAGMKAKCCVLKPHVAELSKLMLNNKTEGISVDAVKSPISGLLVKL
EEEEEECCCEEEEEECCCCCEEEEECCCHHHHHHHHHCCCCCCEEEHHHHCCCCCEEEEE
HVNAGDQVEVGQPLFVVEAMKMENIICAESEMVIKNIPVKEGKNIHAGDLVLDLIS
EECCCCCEECCCCEEEEEHHHHCCEEECCCCEEEECCCCCCCCCCCHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]