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The map label for this gene is mrdA [H]

Identifier: 190570985

GI number: 190570985

Start: 619039

End: 620595

Strand: Reverse

Name: mrdA [H]

Synonym: WPa_0573

Alternate gene names: 190570985

Gene position: 620595-619039 (Counterclockwise)

Preceding gene: 190570986

Following gene: 190570980

Centisome position: 41.86

GC content: 35.39

Gene sequence:

>1557_bases
ATGTGGACAAAAAACAAGGTCTTCAACCGTAGGTCATTCATATTAGGTGGTATTCAGCTTACTATTTCTGCTATTTTTAG
TTGTAGGTTGTATACTTTACAAGTACGAGATAGACAAAAATATGAAGTGCTATCCAATAATAATAGGATAAAAGTTGTTA
CTATTGTGCCTAAACGAGGCAGGATTTTGGATAGGAATAGCGTTGAACTCGCAGTAAACAAAATTTCGTATATTGTTTTA
TTTGATGGGCAAAAAACCTTTACTAGGGAAGTTGATTTGCAGATGTTATCAGAAATTAAACCTGATACAACAAAATCATC
AGATACAAAAATAACTGCTCTTTATAAACGTTATTACCCGTTTGGTTCGATATGTTCTCATATAATCGGATATACAAAAA
AACAGCAAGACATAAATGAAGCAGGGGTTAGTGGAATTGAATATACATATGATCATGTATTAAAAGGCAAGACCGGAAAA
TCTGAGCAGGAAATAAATTCTAAGAAGCGTATCATGAGAGAGTTATCTAGCATACCGCAGCAAGATGGACAAGATATACA
GCTGACAATTGATGTTGATTTACAGAAGAAAATTGCAGAGATATTTAAAGATCACCAAGGTTCCACAGTGGTAATTGATG
TAAATAACGGAGAAATTTTAGCATTATATAATTCACCTTCTTACGATAATAATCTTTTCACTAGCAGATTATCAAATGAA
ACTTGGAAAAATTTAAACAATCCTTCATTACCGCTTGTAAACCGTGCATTATCATATCAAATTCCACCTGGTTCAATATT
TAAAATAATAGATGCGCTTGCGGGTTTAAAAGATGGAATAATAACACCAGAAGAAAAGTTCTCGTGTAGGGGCTATATGA
AAATAGGTGAACGAAAATTTCGTTGCCTAAAAAGCAAAGTCCATGGATATGTATCTTTAAACGAGGCAATGGCCTTCTCA
TGCAACACTTACTTTTATAATATAGGAAAAAAAATAAATGTAGATTCTCTACTAGAAATGGCTAGCAAATTTGGTATAGG
AAGTGGACCATTAATTGGAATGTTTAAGGAAGAAGCTCCGGGATTGTTGCCTGATGTGGATTGGCGTGCACGCAAGCTAT
ATTCAGAGTGGTATTTAGGTGATACCATTAACTTAGTTATAGGACAAGGGTATTTACTTACAACGCCATTACAGCTTGCA
GTTCTTGCAGCGAGGGTTGCAACAGGAAAGGAGGTAATTCCCCACATTGAGATGAGTAAATCAGGGCAAGACTTTCTTGA
TATTGATGTGAATCATGAGCATCTTAGTGTGGTTCGAAAAGCTATGTTTGACGTGGTGAATTCTAACTATAGAAAAGGAC
TAGGCAGTATACAAATTGCTGGCAAAACCGGAACACCAGAGATAAACTCTAAAGGTGAAAGTCATAAATTGTTTATCGCT
TATGGCCCCTACCATGATCCGCGCTATGCAATCTCAGTGTTTATAGAACATGGTAAAACTCCACGCCAAGATGTTGCAAT
TGCTAGTGAGATATTGCAATATATGCTTGAAAAATGA

Upstream 100 bases:

>100_bases
AACTTGTTACCTTAAGTGAGTGGGAAGAAAGGGTTTGTGATGTAGTGAAAGCTAGAAATGTGCCAATAAAAGGAGGATGA
GTGCGTTCTAAAGGAATTCT

Downstream 100 bases:

>100_bases
ACTTATGAAATTGCCATATATGTTACTTGTAATTTATATATTCAAATCAGAGCTTTGAGGTTAAAAAAAAGAAACGAAAA
TTGCATAAACCTTTTCAATT

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MWTKNKVFNRRSFILGGIQLTISAIFSCRLYTLQVRDRQKYEVLSNNNRIKVVTIVPKRGRILDRNSVELAVNKISYIVL
FDGQKTFTREVDLQMLSEIKPDTTKSSDTKITALYKRYYPFGSICSHIIGYTKKQQDINEAGVSGIEYTYDHVLKGKTGK
SEQEINSKKRIMRELSSIPQQDGQDIQLTIDVDLQKKIAEIFKDHQGSTVVIDVNNGEILALYNSPSYDNNLFTSRLSNE
TWKNLNNPSLPLVNRALSYQIPPGSIFKIIDALAGLKDGIITPEEKFSCRGYMKIGERKFRCLKSKVHGYVSLNEAMAFS
CNTYFYNIGKKINVDSLLEMASKFGIGSGPLIGMFKEEAPGLLPDVDWRARKLYSEWYLGDTINLVIGQGYLLTTPLQLA
VLAARVATGKEVIPHIEMSKSGQDFLDIDVNHEHLSVVRKAMFDVVNSNYRKGLGSIQIAGKTGTPEINSKGESHKLFIA
YGPYHDPRYAISVFIEHGKTPRQDVAIASEILQYMLEK

Sequences:

>Translated_518_residues
MWTKNKVFNRRSFILGGIQLTISAIFSCRLYTLQVRDRQKYEVLSNNNRIKVVTIVPKRGRILDRNSVELAVNKISYIVL
FDGQKTFTREVDLQMLSEIKPDTTKSSDTKITALYKRYYPFGSICSHIIGYTKKQQDINEAGVSGIEYTYDHVLKGKTGK
SEQEINSKKRIMRELSSIPQQDGQDIQLTIDVDLQKKIAEIFKDHQGSTVVIDVNNGEILALYNSPSYDNNLFTSRLSNE
TWKNLNNPSLPLVNRALSYQIPPGSIFKIIDALAGLKDGIITPEEKFSCRGYMKIGERKFRCLKSKVHGYVSLNEAMAFS
CNTYFYNIGKKINVDSLLEMASKFGIGSGPLIGMFKEEAPGLLPDVDWRARKLYSEWYLGDTINLVIGQGYLLTTPLQLA
VLAARVATGKEVIPHIEMSKSGQDFLDIDVNHEHLSVVRKAMFDVVNSNYRKGLGSIQIAGKTGTPEINSKGESHKLFIA
YGPYHDPRYAISVFIEHGKTPRQDVAIASEILQYMLEK
>Mature_518_residues
MWTKNKVFNRRSFILGGIQLTISAIFSCRLYTLQVRDRQKYEVLSNNNRIKVVTIVPKRGRILDRNSVELAVNKISYIVL
FDGQKTFTREVDLQMLSEIKPDTTKSSDTKITALYKRYYPFGSICSHIIGYTKKQQDINEAGVSGIEYTYDHVLKGKTGK
SEQEINSKKRIMRELSSIPQQDGQDIQLTIDVDLQKKIAEIFKDHQGSTVVIDVNNGEILALYNSPSYDNNLFTSRLSNE
TWKNLNNPSLPLVNRALSYQIPPGSIFKIIDALAGLKDGIITPEEKFSCRGYMKIGERKFRCLKSKVHGYVSLNEAMAFS
CNTYFYNIGKKINVDSLLEMASKFGIGSGPLIGMFKEEAPGLLPDVDWRARKLYSEWYLGDTINLVIGQGYLLTTPLQLA
VLAARVATGKEVIPHIEMSKSGQDFLDIDVNHEHLSVVRKAMFDVVNSNYRKGLGSIQIAGKTGTPEINSKGESHKLFIA
YGPYHDPRYAISVFIEHGKTPRQDVAIASEILQYMLEK

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=603, Percent_Identity=28.6898839137645, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1786272, Length=401, Percent_Identity=26.1845386533666, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 58483; Mature: 58483

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTKNKVFNRRSFILGGIQLTISAIFSCRLYTLQVRDRQKYEVLSNNNRIKVVTIVPKRG
CCCCCCCCCCCHHEEEHHHHHHHHHHHEEEEEEEECCCCHHHHHCCCCEEEEEEEECCCC
RILDRNSVELAVNKISYIVLFDGQKTFTREVDLQMLSEIKPDTTKSSDTKITALYKRYYP
CEECCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCC
FGSICSHIIGYTKKQQDINEAGVSGIEYTYDHVLKGKTGKSEQEINSKKRIMRELSSIPQ
HHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
QDGQDIQLTIDVDLQKKIAEIFKDHQGSTVVIDVNNGEILALYNSPSYDNNLFTSRLSNE
CCCCEEEEEEECCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCH
TWKNLNNPSLPLVNRALSYQIPPGSIFKIIDALAGLKDGIITPEEKFSCRGYMKIGERKF
HHHCCCCCCCHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
RCLKSKVHGYVSLNEAMAFSCNTYFYNIGKKINVDSLLEMASKFGIGSGPLIGMFKEEAP
HHHHHHHCCEEEECCEEEEEECEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEHHCCC
GLLPDVDWRARKLYSEWYLGDTINLVIGQGYLLTTPLQLAVLAARVATGKEVIPHIEMSK
CCCCCCCHHHHHHHHHHCCCCEEEEEEECCEEEECHHHHHHHHHHHHCCCHHCCCCCCCC
SGQDFLDIDVNHEHLSVVRKAMFDVVNSNYRKGLGSIQIAGKTGTPEINSKGESHKLFIA
CCCCEEEEECCHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEE
YGPYHDPRYAISVFIEHGKTPRQDVAIASEILQYMLEK
ECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWTKNKVFNRRSFILGGIQLTISAIFSCRLYTLQVRDRQKYEVLSNNNRIKVVTIVPKRG
CCCCCCCCCCCHHEEEHHHHHHHHHHHEEEEEEEECCCCHHHHHCCCCEEEEEEEECCCC
RILDRNSVELAVNKISYIVLFDGQKTFTREVDLQMLSEIKPDTTKSSDTKITALYKRYYP
CEECCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCC
FGSICSHIIGYTKKQQDINEAGVSGIEYTYDHVLKGKTGKSEQEINSKKRIMRELSSIPQ
HHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
QDGQDIQLTIDVDLQKKIAEIFKDHQGSTVVIDVNNGEILALYNSPSYDNNLFTSRLSNE
CCCCEEEEEEECCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCH
TWKNLNNPSLPLVNRALSYQIPPGSIFKIIDALAGLKDGIITPEEKFSCRGYMKIGERKF
HHHCCCCCCCHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
RCLKSKVHGYVSLNEAMAFSCNTYFYNIGKKINVDSLLEMASKFGIGSGPLIGMFKEEAP
HHHHHHHCCEEEECCEEEEEECEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEHHCCC
GLLPDVDWRARKLYSEWYLGDTINLVIGQGYLLTTPLQLAVLAARVATGKEVIPHIEMSK
CCCCCCCHHHHHHHHHHCCCCEEEEEEECCEEEECHHHHHHHHHHHHCCCHHCCCCCCCC
SGQDFLDIDVNHEHLSVVRKAMFDVVNSNYRKGLGSIQIAGKTGTPEINSKGESHKLFIA
CCCCEEEEECCHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEE
YGPYHDPRYAISVFIEHGKTPRQDVAIASEILQYMLEK
ECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]