Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 190570951

GI number: 190570951

Start: 573149

End: 574504

Strand: Direct

Name: murD [H]

Synonym: WPa_0533

Alternate gene names: 190570951

Gene position: 573149-574504 (Clockwise)

Preceding gene: 190570950

Following gene: 190570952

Centisome position: 38.66

GC content: 34.88

Gene sequence:

>1356_bases
ATGGTTCGCATAAGTTATCTAGAAGTGCAACTAAACAAATACAAAAATCAAAGTGTTGCGGTTTTCGGCCTTGGTAAAAC
TGGTTTGTCCGTTATTAATGCTCTAACAAAAAGCAGTGCAAAAATATATGCATGGGATGATAATAAAGAGCAAATAGCAA
ATGCAAAAAAGATATATAAAGAGTGTAACTTTACTCATCCCAATGAGTATAATTGGCATGAGATAAGCACACTAATTTTG
AGCCCTGGAGTGCCAATACCAACGCATTGGGTAGTAAAACTTGCAAGAAGCTTTGACTGCAAAATAAAATCAGACATTGA
GCTATTTCTTGAAACTAAAACTACAAATCAGAAGGTTATAGGCATTACAGGAACAAATGGCAAATCAACTACCACATCAC
TAATAGGGCACATATTAAAATCCACAGGGAAAAAAGTAGCTATCGGTGGGAATTTAGGTATGCCTATTTTGGATTTAGAA
AGAGATGCAGAAATTTATGTAATTGAATTCTCCTCTTTTCAATTGGAGCTAATGAATGAAATTAATGTGGATATTTCTGT
ATTGCTTAACATCACACCAGACCACATAGATAGACATGGAAGTATGAATAACTATATAGCAACTAAACTAAAATTGATAA
ACGGTAGCAAGATTGCCGTGATAGGATGTGATAACAAAATTACCTCTGATGTATTCAATAAATTCACTGGGAACAAAATT
CCAATCTCAGCTTCGATGTCACCCTCTTTGTCATCCCAGTGCCTAGGCACTGGGAAACTAGAGAATCATAGCTTATCAGT
AAGCGACGTGAAAATAAACCTAGTATCCAATGTAGAAAATATAGTAGCTGCACACGCTGTGTGCAAGTTACTTGGAGTAG
ATAGCAGCACTATTGTCAATGAAATCAAATCCTTTCCAGGGCTAAGACACAGAAATGAATTTCTTGGCAAAATACATAAT
GTACTTTTTATCAACGATAGCAAAGCAACCAATGCAGAATCGACCGAAAAGGCAATTTTATCTTATAAAAACATATATTG
GATTGTTGGCGGAAAAAGCAAAAAAGGCGGAATAGAATCATTAAGCAAGCATTTCACCAAGATTAGAAAAGCTTTTCTTA
TTGGAGAATCAACTGAAGTTTTTGCAAACATTATGGAGAACAAAATAGATTATATGAAGTGCTATAATCTAGAAAACGCA
TTTAAACTGGCTTTTGAAGAGGCCATAAATAGCAAAGAAGAAGTAGCGATATTACTTTCTCCTGCATGTTCTTCTTTTGA
TCAGTGGAAAAATTTTGAAGAGCGCGGTGAAGCATTTTGCAGAATGTTTGCAAATCTCAGGTACAATTATATGTGA

Upstream 100 bases:

>100_bases
TGTCAAGGAGTTTTCTATTGGCTAAGGTTGTGACAATAAAGTTAAGCACTGCCAAAAACTATGGAAGAAGTCATTATAAT
TTGTATTATATCTTTATAAA

Downstream 100 bases:

>100_bases
TGTTTAGTATAATATTGTATGGAACAAAAGTTAATAGTAGATGAGCTGATTAAGGTTATTCCATTTGAAGGAATAAGTGA
TGCAACCTTATTGAAAGTGT

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MVRISYLEVQLNKYKNQSVAVFGLGKTGLSVINALTKSSAKIYAWDDNKEQIANAKKIYKECNFTHPNEYNWHEISTLIL
SPGVPIPTHWVVKLARSFDCKIKSDIELFLETKTTNQKVIGITGTNGKSTTTSLIGHILKSTGKKVAIGGNLGMPILDLE
RDAEIYVIEFSSFQLELMNEINVDISVLLNITPDHIDRHGSMNNYIATKLKLINGSKIAVIGCDNKITSDVFNKFTGNKI
PISASMSPSLSSQCLGTGKLENHSLSVSDVKINLVSNVENIVAAHAVCKLLGVDSSTIVNEIKSFPGLRHRNEFLGKIHN
VLFINDSKATNAESTEKAILSYKNIYWIVGGKSKKGGIESLSKHFTKIRKAFLIGESTEVFANIMENKIDYMKCYNLENA
FKLAFEEAINSKEEVAILLSPACSSFDQWKNFEERGEAFCRMFANLRYNYM

Sequences:

>Translated_451_residues
MVRISYLEVQLNKYKNQSVAVFGLGKTGLSVINALTKSSAKIYAWDDNKEQIANAKKIYKECNFTHPNEYNWHEISTLIL
SPGVPIPTHWVVKLARSFDCKIKSDIELFLETKTTNQKVIGITGTNGKSTTTSLIGHILKSTGKKVAIGGNLGMPILDLE
RDAEIYVIEFSSFQLELMNEINVDISVLLNITPDHIDRHGSMNNYIATKLKLINGSKIAVIGCDNKITSDVFNKFTGNKI
PISASMSPSLSSQCLGTGKLENHSLSVSDVKINLVSNVENIVAAHAVCKLLGVDSSTIVNEIKSFPGLRHRNEFLGKIHN
VLFINDSKATNAESTEKAILSYKNIYWIVGGKSKKGGIESLSKHFTKIRKAFLIGESTEVFANIMENKIDYMKCYNLENA
FKLAFEEAINSKEEVAILLSPACSSFDQWKNFEERGEAFCRMFANLRYNYM
>Mature_451_residues
MVRISYLEVQLNKYKNQSVAVFGLGKTGLSVINALTKSSAKIYAWDDNKEQIANAKKIYKECNFTHPNEYNWHEISTLIL
SPGVPIPTHWVVKLARSFDCKIKSDIELFLETKTTNQKVIGITGTNGKSTTTSLIGHILKSTGKKVAIGGNLGMPILDLE
RDAEIYVIEFSSFQLELMNEINVDISVLLNITPDHIDRHGSMNNYIATKLKLINGSKIAVIGCDNKITSDVFNKFTGNKI
PISASMSPSLSSQCLGTGKLENHSLSVSDVKINLVSNVENIVAAHAVCKLLGVDSSTIVNEIKSFPGLRHRNEFLGKIHN
VLFINDSKATNAESTEKAILSYKNIYWIVGGKSKKGGIESLSKHFTKIRKAFLIGESTEVFANIMENKIDYMKCYNLENA
FKLAFEEAINSKEEVAILLSPACSSFDQWKNFEERGEAFCRMFANLRYNYM

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=459, Percent_Identity=29.6296296296296, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 50246; Mature: 50246

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRISYLEVQLNKYKNQSVAVFGLGKTGLSVINALTKSSAKIYAWDDNKEQIANAKKIYK
CEEEEEEEEEEECCCCCEEEEEEECHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
ECNFTHPNEYNWHEISTLILSPGVPIPTHWVVKLARSFDCKIKSDIELFLETKTTNQKVI
HCCCCCCCCCCHHHEEEEEECCCCCCCHHHHHHHHHHCCCEECCCEEEEEEECCCCCEEE
GITGTNGKSTTTSLIGHILKSTGKKVAIGGNLGMPILDLERDAEIYVIEFSSFQLELMNE
EEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCEEEEEEECCCEEEEHHH
INVDISVLLNITPDHIDRHGSMNNYIATKLKLINGSKIAVIGCDNKITSDVFNKFTGNKI
CCCEEEEEEECCHHHHHCCCCCCCEEEEEEEEECCCEEEEEECCCCHHHHHHHHHCCCEE
PISASMSPSLSSQCLGTGKLENHSLSVSDVKINLVSNVENIVAAHAVCKLLGVDSSTIVN
EEECCCCCCCHHHHCCCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHH
EIKSFPGLRHRNEFLGKIHNVLFINDSKATNAESTEKAILSYKNIYWIVGGKSKKGGIES
HHHHCCCCCHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHEECCEEEEEECCCCCCCHHH
LSKHFTKIRKAFLIGESTEVFANIMENKIDYMKCYNLENAFKLAFEEAINSKEEVAILLS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEC
PACSSFDQWKNFEERGEAFCRMFANLRYNYM
CCCCCHHHHCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVRISYLEVQLNKYKNQSVAVFGLGKTGLSVINALTKSSAKIYAWDDNKEQIANAKKIYK
CEEEEEEEEEEECCCCCEEEEEEECHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
ECNFTHPNEYNWHEISTLILSPGVPIPTHWVVKLARSFDCKIKSDIELFLETKTTNQKVI
HCCCCCCCCCCHHHEEEEEECCCCCCCHHHHHHHHHHCCCEECCCEEEEEEECCCCCEEE
GITGTNGKSTTTSLIGHILKSTGKKVAIGGNLGMPILDLERDAEIYVIEFSSFQLELMNE
EEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCCEEEEEEECCCEEEEHHH
INVDISVLLNITPDHIDRHGSMNNYIATKLKLINGSKIAVIGCDNKITSDVFNKFTGNKI
CCCEEEEEEECCHHHHHCCCCCCCEEEEEEEEECCCEEEEEECCCCHHHHHHHHHCCCEE
PISASMSPSLSSQCLGTGKLENHSLSVSDVKINLVSNVENIVAAHAVCKLLGVDSSTIVN
EEECCCCCCCHHHHCCCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHH
EIKSFPGLRHRNEFLGKIHNVLFINDSKATNAESTEKAILSYKNIYWIVGGKSKKGGIES
HHHHCCCCCHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHEECCEEEEEECCCCCCCHHH
LSKHFTKIRKAFLIGESTEVFANIMENKIDYMKCYNLENAFKLAFEEAINSKEEVAILLS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEC
PACSSFDQWKNFEERGEAFCRMFANLRYNYM
CCCCCHHHHCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA