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The map label for this gene is ksgA

Identifier: 190570949

GI number: 190570949

Start: 571328

End: 572122

Strand: Reverse

Name: ksgA

Synonym: WPa_0531

Alternate gene names: 190570949

Gene position: 572122-571328 (Counterclockwise)

Preceding gene: 190570953

Following gene: 190570948

Centisome position: 38.59

GC content: 33.46

Gene sequence:

>795_bases
ATGAGAAAATTTTTACTGAAGCCAAAAAAGAGTCTAGGGCAAAATTTTATTTTATCGAATGAGATAATAAAAAGAATAGT
TGCCTTAGCCGGTAGCCTGAAAGATTTTAATGTTATTGAAATTGGTCCTGGGTATGGTGCGTTAACAAGAGAAATATTGG
CATATAATCCAAAGTTTCTACTTTCTATAGAAAAAGATAGTAGTTTAGTGAAACATCACGAACAATTGCTAAATGAGCAT
CAGGGAAAATATAGAATTATAGAAGCAGATGCACTTAATGTTGTAGAAAAAGAGCTGGTAGAATGTCCAGTCAAAGTCAT
TGCTAACTTGCCTTACAATATCTCAGTAGCGTTATTTTTAAAGTGGTTAAATAATATAAAATTTTTTACGAATTTGACGT
TAATGTTCCAAAAGGAGGTAGCAGAGCGTATTACAGCAGAACCTAATTCTAAGGATTATGGTTCCCTATCAGTGCTAAGC
CAGTTACTATGCGACATAAAAAAGGAATTTGATATTGAACCTAAGGAATTTTTTCCAAGACCAAAAGTATATTCTTCAGT
AATTACAGTAAAACCTTTACCCACTCCAAGATTTGCAGTAAATTTAGAAACCTTAACAAAGCTAACACGTGCTGTGTTCG
CTCAAAGAAGAAAGATGCTGAGAAATAGCTTGCAAAGTATAACTAGTGATGTCTCAACTACTCTTGAGAATGCTAAACTG
AGTGGAGACGAACGTCCAGAAAGCTTAACTATTGAGCAGTTCTGTTTGCTAGCAAATAATATAATTATGCGATAA

Upstream 100 bases:

>100_bases
TCTACCAATTGAGCTAAGCCGGCATTAAATACACTGACAATATACAATTATTGATGAATTTTCGTCAACTAATTTTTAAA
ATACTGCATAATAAGATTAT

Downstream 100 bases:

>100_bases
ATATATTAGGAGCTACATACCTCTTCTATCCTATTTTGATCTAAAAATAGCTGGAGCCAGGCTAATTTTGCAAAAGCTCT
ATTGCAATTTCATTAATTGG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRKFLLKPKKSLGQNFILSNEIIKRIVALAGSLKDFNVIEIGPGYGALTREILAYNPKFLLSIEKDSSLVKHHEQLLNEH
QGKYRIIEADALNVVEKELVECPVKVIANLPYNISVALFLKWLNNIKFFTNLTLMFQKEVAERITAEPNSKDYGSLSVLS
QLLCDIKKEFDIEPKEFFPRPKVYSSVITVKPLPTPRFAVNLETLTKLTRAVFAQRRKMLRNSLQSITSDVSTTLENAKL
SGDERPESLTIEQFCLLANNIIMR

Sequences:

>Translated_264_residues
MRKFLLKPKKSLGQNFILSNEIIKRIVALAGSLKDFNVIEIGPGYGALTREILAYNPKFLLSIEKDSSLVKHHEQLLNEH
QGKYRIIEADALNVVEKELVECPVKVIANLPYNISVALFLKWLNNIKFFTNLTLMFQKEVAERITAEPNSKDYGSLSVLS
QLLCDIKKEFDIEPKEFFPRPKVYSSVITVKPLPTPRFAVNLETLTKLTRAVFAQRRKMLRNSLQSITSDVSTTLENAKL
SGDERPESLTIEQFCLLANNIIMR
>Mature_264_residues
MRKFLLKPKKSLGQNFILSNEIIKRIVALAGSLKDFNVIEIGPGYGALTREILAYNPKFLLSIEKDSSLVKHHEQLLNEH
QGKYRIIEADALNVVEKELVECPVKVIANLPYNISVALFLKWLNNIKFFTNLTLMFQKEVAERITAEPNSKDYGSLSVLS
QLLCDIKKEFDIEPKEFFPRPKVYSSVITVKPLPTPRFAVNLETLTKLTRAVFAQRRKMLRNSLQSITSDVSTTLENAKL
SGDERPESLTIEQFCLLANNIIMR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=279, Percent_Identity=34.4086021505376, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI7657198, Length=231, Percent_Identity=32.034632034632, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1786236, Length=258, Percent_Identity=34.8837209302326, Blast_Score=135, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25141369, Length=287, Percent_Identity=28.9198606271777, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25146882, Length=210, Percent_Identity=30.952380952381, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=224, Percent_Identity=28.125, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21357273, Length=278, Percent_Identity=31.294964028777, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21358017, Length=243, Percent_Identity=32.0987654320988, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_WOLPP (B3CPY6)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975307.1
- EnsemblBacteria:   EBWOLT00000000832
- GeneID:   6384430
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0531
- GeneTree:   EBGT00050000031097
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30083; Mature: 30083

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 15-15 BINDING 17-17 BINDING 42-42 BINDING 64-64 BINDING 90-90 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKFLLKPKKSLGQNFILSNEIIKRIVALAGSLKDFNVIEIGPGYGALTREILAYNPKFL
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCEEE
LSIEKDSSLVKHHEQLLNEHQGKYRIIEADALNVVEKELVECPVKVIANLPYNISVALFL
EEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KWLNNIKFFTNLTLMFQKEVAERITAEPNSKDYGSLSVLSQLLCDIKKEFDIEPKEFFPR
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCC
PKVYSSVITVKPLPTPRFAVNLETLTKLTRAVFAQRRKMLRNSLQSITSDVSTTLENAKL
CHHHHHEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SGDERPESLTIEQFCLLANNIIMR
CCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKFLLKPKKSLGQNFILSNEIIKRIVALAGSLKDFNVIEIGPGYGALTREILAYNPKFL
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCEEE
LSIEKDSSLVKHHEQLLNEHQGKYRIIEADALNVVEKELVECPVKVIANLPYNISVALFL
EEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
KWLNNIKFFTNLTLMFQKEVAERITAEPNSKDYGSLSVLSQLLCDIKKEFDIEPKEFFPR
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCC
PKVYSSVITVKPLPTPRFAVNLETLTKLTRAVFAQRRKMLRNSLQSITSDVSTTLENAKL
CHHHHHEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SGDERPESLTIEQFCLLANNIIMR
CCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA