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The map label for this gene is purN [H]

Identifier: 190570923

GI number: 190570923

Start: 541077

End: 541643

Strand: Reverse

Name: purN [H]

Synonym: WPa_0503

Alternate gene names: 190570923

Gene position: 541643-541077 (Counterclockwise)

Preceding gene: 190570925

Following gene: 190570918

Centisome position: 36.54

GC content: 36.68

Gene sequence:

>567_bases
ATGAAAAAGATAAAACTCGGAATACTAATTTCAGGTAGAGGATCAAACATGCAGGCTTTAATAGAAGCATGTCAAGATCA
GAATTTCCCTGCTGAAACTGTGTGTGTTATCACAAATAATAGTGAAGCTGGAGGCCTTAAAATAGCAAAGCAAGCAGGAG
TTTCAGCGTTTGTTGTTGAAGATAAGCCACTTGATACTGATAAAATTCATGAAATACTTGTTCAACATAAGGTTGATTTA
ATTTGCCTTGCAGGGTTTATGAGAATTATAAAGGCCAATTTCCTGAATAAATGGCATAACAAAGTTATAAATATTCATCC
CTCTTTACTTCCGTCATTTAAGGGCCTAAATGCTCAGGAGCAAGCTCTGAAAGCGGGTGTAAAAATTACAGGATGTACTG
TGCATTATGTTACTCCTGAAATTGATGCTGGAGCCATAATCGCTCAAGCTACTGTTCCAGTGTTACCAAATGATGATGTT
CACAGTCTCTCAGAGCGCATTCTAGCTGAAGAGCATAAGTGTTATGTAAAAGCAGTAAGATCAATAGTAGAAGGTATTAA
CTATTGA

Upstream 100 bases:

>100_bases
ATTCAGCAGTGTAAAATATTGCTACACAATAGTATTTATTCTAAAATAAACTTTCCTTTTGAGCTATTTTATTAATATCC
ATTCTTACGGCTGCACTAAA

Downstream 100 bases:

>100_bases
CCTTGAGCGTTTCTTCCAACTTCAACAAAAAATAAATTTTCAGGATTCAGTAAAGAACTTGCCAGCTTGTTTACTTTTTC
CAATTGAACATCATTAATGA

Product: phosphoribosylglycinamide formyltransferase, putative

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MKKIKLGILISGRGSNMQALIEACQDQNFPAETVCVITNNSEAGGLKIAKQAGVSAFVVEDKPLDTDKIHEILVQHKVDL
ICLAGFMRIIKANFLNKWHNKVINIHPSLLPSFKGLNAQEQALKAGVKITGCTVHYVTPEIDAGAIIAQATVPVLPNDDV
HSLSERILAEEHKCYVKAVRSIVEGINY

Sequences:

>Translated_188_residues
MKKIKLGILISGRGSNMQALIEACQDQNFPAETVCVITNNSEAGGLKIAKQAGVSAFVVEDKPLDTDKIHEILVQHKVDL
ICLAGFMRIIKANFLNKWHNKVINIHPSLLPSFKGLNAQEQALKAGVKITGCTVHYVTPEIDAGAIIAQATVPVLPNDDV
HSLSERILAEEHKCYVKAVRSIVEGINY
>Mature_188_residues
MKKIKLGILISGRGSNMQALIEACQDQNFPAETVCVITNNSEAGGLKIAKQAGVSAFVVEDKPLDTDKIHEILVQHKVDL
ICLAGFMRIIKANFLNKWHNKVINIHPSLLPSFKGLNAQEQALKAGVKITGCTVHYVTPEIDAGAIIAQATVPVLPNDDV
HSLSERILAEEHKCYVKAVRSIVEGINY

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=189, Percent_Identity=46.031746031746, Blast_Score=173, Evalue=8e-44,
Organism=Homo sapiens, GI209869995, Length=189, Percent_Identity=46.031746031746, Blast_Score=173, Evalue=8e-44,
Organism=Homo sapiens, GI209869993, Length=189, Percent_Identity=46.031746031746, Blast_Score=173, Evalue=8e-44,
Organism=Escherichia coli, GI1788846, Length=183, Percent_Identity=36.0655737704918, Blast_Score=137, Evalue=3e-34,
Organism=Escherichia coli, GI1787483, Length=87, Percent_Identity=40.2298850574713, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=188, Percent_Identity=38.2978723404255, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320616, Length=211, Percent_Identity=26.5402843601896, Blast_Score=68, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24582400, Length=191, Percent_Identity=45.5497382198953, Blast_Score=177, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20490; Mature: 20490

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIKLGILISGRGSNMQALIEACQDQNFPAETVCVITNNSEAGGLKIAKQAGVSAFVVE
CCEEEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEHHHCCCEEEEEE
DKPLDTDKIHEILVQHKVDLICLAGFMRIIKANFLNKWHNKVINIHPSLLPSFKGLNAQE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHCCCCCCCCHHH
QALKAGVKITGCTVHYVTPEIDAGAIIAQATVPVLPNDDVHSLSERILAEEHKCYVKAVR
HHHHCCCEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
SIVEGINY
HHHHHCCC
>Mature Secondary Structure
MKKIKLGILISGRGSNMQALIEACQDQNFPAETVCVITNNSEAGGLKIAKQAGVSAFVVE
CCEEEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEHHHCCCEEEEEE
DKPLDTDKIHEILVQHKVDLICLAGFMRIIKANFLNKWHNKVINIHPSLLPSFKGLNAQE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHCCCCCCCCHHH
QALKAGVKITGCTVHYVTPEIDAGAIIAQATVPVLPNDDVHSLSERILAEEHKCYVKAVR
HHHHCCCEEEEEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
SIVEGINY
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]