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The map label for this gene is cisA [H]

Identifier: 190570740

GI number: 190570740

Start: 320006

End: 321487

Strand: Reverse

Name: cisA [H]

Synonym: WPa_0297

Alternate gene names: 190570740

Gene position: 321487-320006 (Counterclockwise)

Preceding gene: 190570741

Following gene: 190570736

Centisome position: 21.69

GC content: 34.35

Gene sequence:

>1482_bases
ATGCTAAAAGAAGTAAGATGTGCAATATATACGAGAAAATCAAATGAAGATGGTCTAGAACAAAAGTTTAATAGTCTTGA
TGCGCAACGTGTAGCATGTGAAAAATATATAAAAAGCCGAGAAGGATGGGTAGCATTGGCAAAAAGGTACGATGATGGCG
GATATTCAGGAAAAAACTTAGAAAGACCAGCAATAAAAGAATTATTTGAAGATGTAAAGGCAGGAGAAGTAGATTGTGTA
GTAGTATATACGCTCGATAGGCTATCAAGAGAAACAAAAGACAGCATAGAAGTGACATCATTTTTTAGAAGGCACCGAGT
AAATTTTATAGCAGTAACGCAGATATTTGATAATAACACACCAATGGGAAAATTTGTACAAACGGTATTATCAGGAGCAG
CGCAACTAGAAAGAGAAATGATCGTAGAAAGAGTAAAAAATAAAATAGCAACATCAAAAGAACAAGGATTATGGATGGGT
GGAACTTTACCGCTTGGGTATGATGTGAAAGATAAGGAATTAATAATAAATGGAAAAGAAGCAAAGACGGTAAAACATAT
ATTTGAGAGGTATATGGAGCTAAAATCAATGGCAGAATTGGCAAGGGAGTTAAATAGTCAAGGTTACAGAACGAAAGCAA
GGTTTGATATCTTTAAAAAAGCAACGGTGAGGAGAATAATAACAAATCCAATATATATGGGAAAAATACGGCATTATGAG
AAAGAGTATGAAGGAAAACATGAAGCAATAATAGAAGAGGAAAAGTGGCAAAAAGCACAAGAATTAATAAGGAATCAGCC
ATATAGAAAAGCAAAATATGAGGAAGCGCTGCTTAGGGGAATAATTAAGTGCAAAAGCTGTGATGTAAATATGACTTTGA
CGTACTCAAAAAAAGAGAATAAAAGGTACCGATATTATATATGTAATAACCATTTAAGAGGAAAAAGTTGTGAATCGGTA
AATCGAACAGTAGTAGCAGGAAAAGTGGAAAAAGAAGTGATGAAAAGAGCAGAAGATTTATATGAAAAATGTGGAGAAGA
ATGGAAAAATTTAAGTTTTGGAAAACAGAAAGAAGTAGTGAAAAAGTTAATAAAGGGAGTAATGGTAAAAGAAGATGGAA
TAGAGGTGAGCTCAGAGGATAAGGTGGAATTTATACCAATAAAAAAGAAAGGAAACAAATGCACAGTAGTAGAGCCAGAG
GGGAAAACAAACAATGCATTACTGAAAGCAGTGGTGAGGGCTCACCTATGGAAACGTCAGCTAGAGGAAGGAAAATACGC
AAATATAAAAGAGCTGAGTGCTAAAGTTAATATAGGTACAAGACGTATACAACAAATTTTAAGATTAAATTATTTAGCAC
CAAAAATTAAAGAAGATATAGTAAATGGGAGGCAGCCAAGGGGTTTGAGGTTAGTGGATTTAAGAGAAATACCGATGCTG
TGGGGTGAGCAATTAGAGAAATTTTATGGATTAGGGGCGTAA

Upstream 100 bases:

>100_bases
TACGTCATTGTCAGCAGTAGCCGGGAAAATAATGGGAATGAGCTACAACGGACCTTTATTATTCGGGATGCGTGACAAAA
GTGGAAATTGAGGAGAGAGA

Downstream 100 bases:

>100_bases
TTACACCATTTATAAAAAGAACAAAAGGAGAGAAACTGAAAACACCAAAAACTATTGAAAGGTATTGCTGAGAAATAGGA
AAAATAGCCAAATTGCCGCT

Product: site-specific recombinase, resolvase family

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MLKEVRCAIYTRKSNEDGLEQKFNSLDAQRVACEKYIKSREGWVALAKRYDDGGYSGKNLERPAIKELFEDVKAGEVDCV
VVYTLDRLSRETKDSIEVTSFFRRHRVNFIAVTQIFDNNTPMGKFVQTVLSGAAQLEREMIVERVKNKIATSKEQGLWMG
GTLPLGYDVKDKELIINGKEAKTVKHIFERYMELKSMAELARELNSQGYRTKARFDIFKKATVRRIITNPIYMGKIRHYE
KEYEGKHEAIIEEEKWQKAQELIRNQPYRKAKYEEALLRGIIKCKSCDVNMTLTYSKKENKRYRYYICNNHLRGKSCESV
NRTVVAGKVEKEVMKRAEDLYEKCGEEWKNLSFGKQKEVVKKLIKGVMVKEDGIEVSSEDKVEFIPIKKKGNKCTVVEPE
GKTNNALLKAVVRAHLWKRQLEEGKYANIKELSAKVNIGTRRIQQILRLNYLAPKIKEDIVNGRQPRGLRLVDLREIPML
WGEQLEKFYGLGA

Sequences:

>Translated_493_residues
MLKEVRCAIYTRKSNEDGLEQKFNSLDAQRVACEKYIKSREGWVALAKRYDDGGYSGKNLERPAIKELFEDVKAGEVDCV
VVYTLDRLSRETKDSIEVTSFFRRHRVNFIAVTQIFDNNTPMGKFVQTVLSGAAQLEREMIVERVKNKIATSKEQGLWMG
GTLPLGYDVKDKELIINGKEAKTVKHIFERYMELKSMAELARELNSQGYRTKARFDIFKKATVRRIITNPIYMGKIRHYE
KEYEGKHEAIIEEEKWQKAQELIRNQPYRKAKYEEALLRGIIKCKSCDVNMTLTYSKKENKRYRYYICNNHLRGKSCESV
NRTVVAGKVEKEVMKRAEDLYEKCGEEWKNLSFGKQKEVVKKLIKGVMVKEDGIEVSSEDKVEFIPIKKKGNKCTVVEPE
GKTNNALLKAVVRAHLWKRQLEEGKYANIKELSAKVNIGTRRIQQILRLNYLAPKIKEDIVNGRQPRGLRLVDLREIPML
WGEQLEKFYGLGA
>Mature_493_residues
MLKEVRCAIYTRKSNEDGLEQKFNSLDAQRVACEKYIKSREGWVALAKRYDDGGYSGKNLERPAIKELFEDVKAGEVDCV
VVYTLDRLSRETKDSIEVTSFFRRHRVNFIAVTQIFDNNTPMGKFVQTVLSGAAQLEREMIVERVKNKIATSKEQGLWMG
GTLPLGYDVKDKELIINGKEAKTVKHIFERYMELKSMAELARELNSQGYRTKARFDIFKKATVRRIITNPIYMGKIRHYE
KEYEGKHEAIIEEEKWQKAQELIRNQPYRKAKYEEALLRGIIKCKSCDVNMTLTYSKKENKRYRYYICNNHLRGKSCESV
NRTVVAGKVEKEVMKRAEDLYEKCGEEWKNLSFGKQKEVVKKLIKGVMVKEDGIEVSSEDKVEFIPIKKKGNKCTVVEPE
GKTNNALLKAVVRAHLWKRQLEEGKYANIKELSAKVNIGTRRIQQILRLNYLAPKIKEDIVNGRQPRGLRLVDLREIPML
WGEQLEKFYGLGA

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=109, Percent_Identity=39.4495412844037, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 57220; Mature: 57220

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEVRCAIYTRKSNEDGLEQKFNSLDAQRVACEKYIKSREGWVALAKRYDDGGYSGKNL
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCC
ERPAIKELFEDVKAGEVDCVVVYTLDRLSRETKDSIEVTSFFRRHRVNFIAVTQIFDNNT
CCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCC
PMGKFVQTVLSGAAQLEREMIVERVKNKIATSKEQGLWMGGTLPLGYDVKDKELIINGKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCEEECCCCCCCCCCCCCEEEEECHH
AKTVKHIFERYMELKSMAELARELNSQGYRTKARFDIFKKATVRRIITNPIYMGKIRHYE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KEYEGKHEAIIEEEKWQKAQELIRNQPYRKAKYEEALLRGIIKCKSCDVNMTLTYSKKEN
HHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
KRYRYYICNNHLRGKSCESVNRTVVAGKVEKEVMKRAEDLYEKCGEEWKNLSFGKQKEVV
CEEEEEEECCCCCCCCCHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KKLIKGVMVKEDGIEVSSEDKVEFIPIKKKGNKCTVVEPEGKTNNALLKAVVRAHLWKRQ
HHHHHHHEECCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
LEEGKYANIKELSAKVNIGTRRIQQILRLNYLAPKIKEDIVNGRQPRGLRLVDLREIPML
HHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEECHHHHHHH
WGEQLEKFYGLGA
HHHHHHHHHCCCC
>Mature Secondary Structure
MLKEVRCAIYTRKSNEDGLEQKFNSLDAQRVACEKYIKSREGWVALAKRYDDGGYSGKNL
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCC
ERPAIKELFEDVKAGEVDCVVVYTLDRLSRETKDSIEVTSFFRRHRVNFIAVTQIFDNNT
CCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCC
PMGKFVQTVLSGAAQLEREMIVERVKNKIATSKEQGLWMGGTLPLGYDVKDKELIINGKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCEEECCCCCCCCCCCCCEEEEECHH
AKTVKHIFERYMELKSMAELARELNSQGYRTKARFDIFKKATVRRIITNPIYMGKIRHYE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
KEYEGKHEAIIEEEKWQKAQELIRNQPYRKAKYEEALLRGIIKCKSCDVNMTLTYSKKEN
HHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
KRYRYYICNNHLRGKSCESVNRTVVAGKVEKEVMKRAEDLYEKCGEEWKNLSFGKQKEVV
CEEEEEEECCCCCCCCCHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KKLIKGVMVKEDGIEVSSEDKVEFIPIKKKGNKCTVVEPEGKTNNALLKAVVRAHLWKRQ
HHHHHHHEECCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
LEEGKYANIKELSAKVNIGTRRIQQILRLNYLAPKIKEDIVNGRQPRGLRLVDLREIPML
HHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEEECHHHHHHH
WGEQLEKFYGLGA
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]