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The map label for this gene is lipA

Identifier: 190570685

GI number: 190570685

Start: 244878

End: 245750

Strand: Direct

Name: lipA

Synonym: WPa_0235

Alternate gene names: 190570685

Gene position: 244878-245750 (Clockwise)

Preceding gene: 190570684

Following gene: 190570686

Centisome position: 16.52

GC content: 37.11

Gene sequence:

>873_bases
ATGCATAGTAAGCCTACATGGCTCAGAGCAAAAGCTCCAGCTGGTAAAGTATTCAATGAAACCTTAAACACTGTTAAGCT
ACATAACTTACATACAGTATGCGAAGAAGCTGCGTGTCCAAATATTGGTGAATGTTGGAATAAACGTCATGCTACTGTGA
TGATTCTCGGTTCTGTTTGTACTCGTGCTTGTGCGTTTTGCAACGTTGCAACTGGCATTCCTGATAAGCTCGATCCTCAT
GAACCAGAAAATTTAGCAAAAGCAATAAAAAAGTTAAATTTAAAGCACGTTGTCATTACCTCTGTTGACCGTGACGATTT
ATCAGATGGTGGTGCAAATCAGTTTATAAGGTGTATAGAAGAAATTAGAAAGATAACTTCGGAAACAACAATAGAGATTC
TCACTCCTGATTTTTTAAATAAGAAAGGAGCATTTGAAGCAATAGCTATTGCATCACCTGATGTTTATAACCACAACATC
GAAACAGTACCAAGGCTGTATGCAAAAATAAGACCAAGAGCTCGTTATTTTCATTCACTATATTTGCTAAAGATGGTGAA
ACAGATTAATCCTAAACTTTTCACGAAGTCAGGGCTTATGGTTGGTCTTGGAGAAACAAAAGAAGAAATCTTTCAGGTTA
TGGATGATTTACGTAGCGCCGAAGTTGATTTCATTACAATTGGTCAATATCTGCAACCAACTCCAAAGCATGCAAAAATT
GATAGATATGTTACTCCAGAAGAGTTTGAGCATTATAAATATATTGCTTATTCTAAAGGCTTCTTGGTAGTTGCATCAAG
CCCATTAACGCGGTCATCGTACCACGCTGAAGAAGATTTTAACAGACTTGCTGCTGCTAAAACTTCAACCTAA

Upstream 100 bases:

>100_bases
CTCAACACAAAAACAATCAAACTAAGTAAAGAAGCGCAAAAGATAAAAAGAAGACTAAAGAAAGTTTTAGCAAAACAAGA
GGTAGAGCTAGCTGTTTCAG

Downstream 100 bases:

>100_bases
TTTAACCTATCTGTTCAAAGAAATGTGCACTCCCTTTCTTTTCATTCAAGTAGCCTCTTATCCTTCCAGTAGCCCATCGG
TGTTATTTCAGTAACACCTG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MHSKPTWLRAKAPAGKVFNETLNTVKLHNLHTVCEEAACPNIGECWNKRHATVMILGSVCTRACAFCNVATGIPDKLDPH
EPENLAKAIKKLNLKHVVITSVDRDDLSDGGANQFIRCIEEIRKITSETTIEILTPDFLNKKGAFEAIAIASPDVYNHNI
ETVPRLYAKIRPRARYFHSLYLLKMVKQINPKLFTKSGLMVGLGETKEEIFQVMDDLRSAEVDFITIGQYLQPTPKHAKI
DRYVTPEEFEHYKYIAYSKGFLVVASSPLTRSSYHAEEDFNRLAAAKTST

Sequences:

>Translated_290_residues
MHSKPTWLRAKAPAGKVFNETLNTVKLHNLHTVCEEAACPNIGECWNKRHATVMILGSVCTRACAFCNVATGIPDKLDPH
EPENLAKAIKKLNLKHVVITSVDRDDLSDGGANQFIRCIEEIRKITSETTIEILTPDFLNKKGAFEAIAIASPDVYNHNI
ETVPRLYAKIRPRARYFHSLYLLKMVKQINPKLFTKSGLMVGLGETKEEIFQVMDDLRSAEVDFITIGQYLQPTPKHAKI
DRYVTPEEFEHYKYIAYSKGFLVVASSPLTRSSYHAEEDFNRLAAAKTST
>Mature_290_residues
MHSKPTWLRAKAPAGKVFNETLNTVKLHNLHTVCEEAACPNIGECWNKRHATVMILGSVCTRACAFCNVATGIPDKLDPH
EPENLAKAIKKLNLKHVVITSVDRDDLSDGGANQFIRCIEEIRKITSETTIEILTPDFLNKKGAFEAIAIASPDVYNHNI
ETVPRLYAKIRPRARYFHSLYLLKMVKQINPKLFTKSGLMVGLGETKEEIFQVMDDLRSAEVDFITIGQYLQPTPKHAKI
DRYVTPEEFEHYKYIAYSKGFLVVASSPLTRSSYHAEEDFNRLAAAKTST

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=293, Percent_Identity=46.0750853242321, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=46.7213114754098, Blast_Score=241, Evalue=7e-64,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=50, Blast_Score=280, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI32564533, Length=254, Percent_Identity=47.6377952755905, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324770, Length=283, Percent_Identity=46.9964664310954, Blast_Score=263, Evalue=3e-71,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=47.3498233215548, Blast_Score=257, Evalue=7e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_WOLPP (B3CP16)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975043.1
- EnsemblBacteria:   EBWOLT00000001173
- GeneID:   6384759
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0235
- GeneTree:   EBGT00050000030887
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 32679; Mature: 32679

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSKPTWLRAKAPAGKVFNETLNTVKLHNLHTVCEEAACPNIGECWNKRHATVMILGSVC
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEHHHHH
TRACAFCNVATGIPDKLDPHEPENLAKAIKKLNLKHVVITSVDRDDLSDGGANQFIRCIE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHH
EIRKITSETTIEILTPDFLNKKGAFEAIAIASPDVYNHNIETVPRLYAKIRPRARYFHSL
HHHHHHCCCEEEEECHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
YLLKMVKQINPKLFTKSGLMVGLGETKEEIFQVMDDLRSAEVDFITIGQYLQPTPKHAKI
HHHHHHHHHCCCEEECCCCEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCC
DRYVTPEEFEHYKYIAYSKGFLVVASSPLTRSSYHAEEDFNRLAAAKTST
CCCCCHHHHHHEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHSKPTWLRAKAPAGKVFNETLNTVKLHNLHTVCEEAACPNIGECWNKRHATVMILGSVC
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEHHHHH
TRACAFCNVATGIPDKLDPHEPENLAKAIKKLNLKHVVITSVDRDDLSDGGANQFIRCIE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHH
EIRKITSETTIEILTPDFLNKKGAFEAIAIASPDVYNHNIETVPRLYAKIRPRARYFHSL
HHHHHHCCCEEEEECHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
YLLKMVKQINPKLFTKSGLMVGLGETKEEIFQVMDDLRSAEVDFITIGQYLQPTPKHAKI
HHHHHHHHHCCCEEECCCCEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCC
DRYVTPEEFEHYKYIAYSKGFLVVASSPLTRSSYHAEEDFNRLAAAKTST
CCCCCHHHHHHEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA