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The map label for this gene is dnaG [H]

Identifier: 190570640

GI number: 190570640

Start: 191333

End: 193066

Strand: Reverse

Name: dnaG [H]

Synonym: WPa_0182

Alternate gene names: 190570640

Gene position: 193066-191333 (Counterclockwise)

Preceding gene: 190570644

Following gene: 190570639

Centisome position: 13.02

GC content: 34.95

Gene sequence:

>1734_bases
ATGGATCATATAGATATTATAAAGTCAAAATTATTGTTGTCCGACATAGTAGGAAAAAAAGTTAGGTTAATAAAAAGAGG
AGATAGTTTTGTTGGACTCTGCCCATTTCATAACGAAAAAACTCCATCTTTTTCGGTCAGCAATGTTAAGGGCTTATATT
ACTGTTTCGGTTGCTCAGCTCATGGTGATGCGTTTGAATTTATTTCACAAACTGAGGGCTTAAGTTTTAAGGAAGCGTTG
GAAAATTTGGCATCTGTTGCAGGTGTTGAGTTACCCAAAAACCTCAGCTTTGCTAAAGAAAATGACAAACTGTTTTCAGC
ACTGAATTTAGCTGCAAGTTGGTTCGCGCAGAAAAATCGAGGTGTTATGGCTTATTTAAGGCAGCGCAAGATCTCACTCA
AAATCATAGATAAATTTAGAATAGGTTATGCACCAAGTTCTGGCTTGAAGGAATATTTGAACTCCTCTGGTATTAAAGAC
GAGATTTTGATCGATGTTGGGTTAATAAATAAGAATTCTCGTGATTATTTCTATGATCGGCTGATATTTCCTATACACAA
CATCACGGGCAAAGTTATTGGTTTTGGCGGACGTGCGCTGAACTCTGAGCAACAGCCGAAGTATCTAAACAGCCCAGAGA
GTCAGCTCTTTAAGAAAAGAGAAAATTTATATGGATTAAACTTTGCTTTGAGTGAAATACGCAAAAAACAGCATCTATTT
GTTGTTGAAGGATATATGGATGTAATAGCACTACATCAAGCGGGAATTAGCAATACAGTTGCTCCGCTTGGTACCGCAAT
TTCTGCAGAGCAGATAAAAAATCTATGGAAATTTGCTAAAGAAATTTCCATCTGTATGGATGGCGATAGTGCCGGACGTC
ATGCTGCTATTCGAATTGCAGAACTTGTTCTACCGATACTGGAGCCTGGATACACATTGAAGTTCGTAACTTTGCCAAAC
AATAAGGACCCATACGATATATGCAATGAGCTAGAATATAAGACAGAAGACGTACTCTCTGCTTTTAACCACTCAACAGA
ACTGCACTCTGAATATTTATGGCATCACATAATTGGTAGAAATTTGCAAAATTATGAAAAATTTGCTCCGGAAAAATATT
CAATTCTTGAGCACAAGTTCATGAAATATGTCAATGCTATCGGTAACAGCAGTATTAGAAGGTATTATAGAGATTATTTT
TACAATAAGGTCAGTGAATTAAGAAGAAACTTTAAAAAGCTGATTTTTAATAGCAAAGCAACAAAAGGTGAGTATCTTTA
CAATAAATCTCCAGAGCTAATTGAAGCAGAGCAAAATCAGGCCATAATTTTGCGTATAGCCGTAGAATTTCCTGAAATCT
TGAACCGTCCTATATTTTTTGAGCAATTTTCTCATTTCGAATTTACTAATGAGATGAAAAAATTACAACAGTGCGTAATT
AATATGGTAACTAATGAGAGCAACTTTAATAAAGAAATTCTGCTACAGGAGTCTAGTGTTATAAAGTTTATATTTGAAAA
AACTAGCGTACTAAATAGTCAATTAAGTGAGAAGAGATCGGCAGAAACTGTCTGGAATAACATAGTATTACGGAAAGAAT
TAAATGTATTACAAGAAGAAAAAATTAAAGCAAGGCTGGGCGGTAATTTTGACTTAGAGGAAAGGCTAATAGAACAAATA
AAGCAAATAGAGGATAGTATACAAGAAATGCAAATGGAATTTATTCAAAAGTAG

Upstream 100 bases:

>100_bases
GAGCTTTTTGTCAAGCTCTTCTGATCTTTCTGAATAAATTTCGAGTCAGATATGCATAAATTCTATATAATTTTTATCCT
ACCACTACTCATTCGGTAAA

Downstream 100 bases:

>100_bases
ATGGCTTAAGATGTTCAAAATTATTAGAAAAACACTGTATTAGTATTTGCTTAAAATAGCTTATTCTAGTATATTTCTGG
TTTGTTCAACTACGATAAGT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MDHIDIIKSKLLLSDIVGKKVRLIKRGDSFVGLCPFHNEKTPSFSVSNVKGLYYCFGCSAHGDAFEFISQTEGLSFKEAL
ENLASVAGVELPKNLSFAKENDKLFSALNLAASWFAQKNRGVMAYLRQRKISLKIIDKFRIGYAPSSGLKEYLNSSGIKD
EILIDVGLINKNSRDYFYDRLIFPIHNITGKVIGFGGRALNSEQQPKYLNSPESQLFKKRENLYGLNFALSEIRKKQHLF
VVEGYMDVIALHQAGISNTVAPLGTAISAEQIKNLWKFAKEISICMDGDSAGRHAAIRIAELVLPILEPGYTLKFVTLPN
NKDPYDICNELEYKTEDVLSAFNHSTELHSEYLWHHIIGRNLQNYEKFAPEKYSILEHKFMKYVNAIGNSSIRRYYRDYF
YNKVSELRRNFKKLIFNSKATKGEYLYNKSPELIEAEQNQAIILRIAVEFPEILNRPIFFEQFSHFEFTNEMKKLQQCVI
NMVTNESNFNKEILLQESSVIKFIFEKTSVLNSQLSEKRSAETVWNNIVLRKELNVLQEEKIKARLGGNFDLEERLIEQI
KQIEDSIQEMQMEFIQK

Sequences:

>Translated_577_residues
MDHIDIIKSKLLLSDIVGKKVRLIKRGDSFVGLCPFHNEKTPSFSVSNVKGLYYCFGCSAHGDAFEFISQTEGLSFKEAL
ENLASVAGVELPKNLSFAKENDKLFSALNLAASWFAQKNRGVMAYLRQRKISLKIIDKFRIGYAPSSGLKEYLNSSGIKD
EILIDVGLINKNSRDYFYDRLIFPIHNITGKVIGFGGRALNSEQQPKYLNSPESQLFKKRENLYGLNFALSEIRKKQHLF
VVEGYMDVIALHQAGISNTVAPLGTAISAEQIKNLWKFAKEISICMDGDSAGRHAAIRIAELVLPILEPGYTLKFVTLPN
NKDPYDICNELEYKTEDVLSAFNHSTELHSEYLWHHIIGRNLQNYEKFAPEKYSILEHKFMKYVNAIGNSSIRRYYRDYF
YNKVSELRRNFKKLIFNSKATKGEYLYNKSPELIEAEQNQAIILRIAVEFPEILNRPIFFEQFSHFEFTNEMKKLQQCVI
NMVTNESNFNKEILLQESSVIKFIFEKTSVLNSQLSEKRSAETVWNNIVLRKELNVLQEEKIKARLGGNFDLEERLIEQI
KQIEDSIQEMQMEFIQK
>Mature_577_residues
MDHIDIIKSKLLLSDIVGKKVRLIKRGDSFVGLCPFHNEKTPSFSVSNVKGLYYCFGCSAHGDAFEFISQTEGLSFKEAL
ENLASVAGVELPKNLSFAKENDKLFSALNLAASWFAQKNRGVMAYLRQRKISLKIIDKFRIGYAPSSGLKEYLNSSGIKD
EILIDVGLINKNSRDYFYDRLIFPIHNITGKVIGFGGRALNSEQQPKYLNSPESQLFKKRENLYGLNFALSEIRKKQHLF
VVEGYMDVIALHQAGISNTVAPLGTAISAEQIKNLWKFAKEISICMDGDSAGRHAAIRIAELVLPILEPGYTLKFVTLPN
NKDPYDICNELEYKTEDVLSAFNHSTELHSEYLWHHIIGRNLQNYEKFAPEKYSILEHKFMKYVNAIGNSSIRRYYRDYF
YNKVSELRRNFKKLIFNSKATKGEYLYNKSPELIEAEQNQAIILRIAVEFPEILNRPIFFEQFSHFEFTNEMKKLQQCVI
NMVTNESNFNKEILLQESSVIKFIFEKTSVLNSQLSEKRSAETVWNNIVLRKELNVLQEEKIKARLGGNFDLEERLIEQI
KQIEDSIQEMQMEFIQK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=468, Percent_Identity=32.4786324786325, Blast_Score=240, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 66476; Mature: 66476

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHIDIIKSKLLLSDIVGKKVRLIKRGDSFVGLCPFHNEKTPSFSVSNVKGLYYCFGCSA
CCHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCC
HGDAFEFISQTEGLSFKEALENLASVAGVELPKNLSFAKENDKLFSALNLAASWFAQKNR
CCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GVMAYLRQRKISLKIIDKFRIGYAPSSGLKEYLNSSGIKDEILIDVGLINKNSRDYFYDR
CHHHHHHHHCCCEEEEEHHHCCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHH
LIFPIHNITGKVIGFGGRALNSEQQPKYLNSPESQLFKKRENLYGLNFALSEIRKKQHLF
HHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCEE
VVEGYMDVIALHQAGISNTVAPLGTAISAEQIKNLWKFAKEISICMDGDSAGRHAAIRIA
EEEHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHH
ELVLPILEPGYTLKFVTLPNNKDPYDICNELEYKTEDVLSAFNHSTELHSEYLWHHIIGR
HHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
NLQNYEKFAPEKYSILEHKFMKYVNAIGNSSIRRYYRDYFYNKVSELRRNFKKLIFNSKA
CHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TKGEYLYNKSPELIEAEQNQAIILRIAVEFPEILNRPIFFEQFSHFEFTNEMKKLQQCVI
CCCCCCCCCCCCCEECCCCCEEEEEEEEHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHH
NMVTNESNFNKEILLQESSVIKFIFEKTSVLNSQLSEKRSAETVWNNIVLRKELNVLQEE
HHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIKARLGGNFDLEERLIEQIKQIEDSIQEMQMEFIQK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDHIDIIKSKLLLSDIVGKKVRLIKRGDSFVGLCPFHNEKTPSFSVSNVKGLYYCFGCSA
CCHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCC
HGDAFEFISQTEGLSFKEALENLASVAGVELPKNLSFAKENDKLFSALNLAASWFAQKNR
CCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GVMAYLRQRKISLKIIDKFRIGYAPSSGLKEYLNSSGIKDEILIDVGLINKNSRDYFYDR
CHHHHHHHHCCCEEEEEHHHCCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHH
LIFPIHNITGKVIGFGGRALNSEQQPKYLNSPESQLFKKRENLYGLNFALSEIRKKQHLF
HHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCEE
VVEGYMDVIALHQAGISNTVAPLGTAISAEQIKNLWKFAKEISICMDGDSAGRHAAIRIA
EEEHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHH
ELVLPILEPGYTLKFVTLPNNKDPYDICNELEYKTEDVLSAFNHSTELHSEYLWHHIIGR
HHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
NLQNYEKFAPEKYSILEHKFMKYVNAIGNSSIRRYYRDYFYNKVSELRRNFKKLIFNSKA
CHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TKGEYLYNKSPELIEAEQNQAIILRIAVEFPEILNRPIFFEQFSHFEFTNEMKKLQQCVI
CCCCCCCCCCCCCEECCCCCEEEEEEEEHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHH
NMVTNESNFNKEILLQESSVIKFIFEKTSVLNSQLSEKRSAETVWNNIVLRKELNVLQEE
HHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIKARLGGNFDLEERLIEQIKQIEDSIQEMQMEFIQK
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]