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The map label for this gene is cca [H]

Identifier: 190570623

GI number: 190570623

Start: 170916

End: 172142

Strand: Reverse

Name: cca [H]

Synonym: WPa_0164

Alternate gene names: 190570623

Gene position: 172142-170916 (Counterclockwise)

Preceding gene: 190570624

Following gene: 190570618

Centisome position: 11.61

GC content: 35.29

Gene sequence:

>1227_bases
ATGCAAATTGATCACGAAACTAGTGTAATTATTGAGGCCATAGAGAAATTTGGTGGTGAGGCAAGGCTTGTTGGTGGATG
TGTTAGAGATTCAATTTTACAGCGTGACATTCACGACATCGATCTTGCTACTAATCTGCTGCCTAATCAAGCAGTTAAAG
CGTTAAAACTCCGTAATATAAAAACTATTCCAACTGGCTTAAAACATGGAACTATTACTGCGATTTTAAATAAAAGATCC
TTTGAGATTACAACGCTAAGGCATGATGTAAAGTGTGACGGTAGACATGCGAAGGTAGAATTTACTAATGATTGGCAAGC
TGATGCTTCAAGGCGCGACTTTACGTTTAATGCTCTTTATGCAGACAAATATGGCCATATATACGACTACTTTGGTGGCA
TTCAGGACTTAAAAGCACGAAAGTTAAACTTTATAGGTAACGCTGAAGATAGGATTAAGGAAGACTATCTACGTATTTTA
AGAGCGTTTCGTTTTCACGCTAAAATATGCATAGGAGATTTAAGTGATGAAATATTGGATGTGTGCAAAAAGCATTCGCA
CATGATTCAAAACCTCTCTGGAGAGAGAATAAGAGAAGAAATATTTAAACTCTTGGAATGCAATGATCCTGCTCCAACAC
TTAAGAGCATGCAAAAATCTGATGTTTTACAAAAAATTATTCCAAAAGAAGTAAAATGCGAAATTTTATCTTCACCACTT
CTTATCAACACTAAACCTCCTACAAAACCCTCAGCGTATGGCCATACGCCTTTCCTAGATCCCACAATCGATGCACTAAC
AAAATTAGCGTTGCTTATTAGGACTGCTGAAGATAGAGTTAGTCTTGGAGAAGAAGTGAGCAAGTTTTTACGTCTTTCAA
ATAAGCAAAAGAAAAAGCTATTATTTTTATTATCAAACGATATCAAAACAGAGCTTTCAGAAAAAGAGCAAAAGAAATAC
ATATCTTTATTTGGTAAGGAATTATATTGTGACTTAATGAAGATTTGTGGTATTGAGTCTGGAACAAATGTTGATGAGTA
TATTTCATTTGCTCATATATTCAATATTCCAAAATTTCCTTTATCTGGGGATGATCTAATAATTATAGGTCACCAGCCAG
GAAAAAATTTAGGTAGAAACTTGGAATTGCTACGACAACACTGGGAAGACAGCTCCTACACTTTAACAAAGGAGGAGCTG
TTGCTTTATGCTAAAAGTTTTATCTAG

Upstream 100 bases:

>100_bases
CCTCTTATTTTACCACTCTCCTGCCTATATATTTTTATATGAGGATTTTTGCAAAAGTTTCGCATTTTTTGTAACATGAT
TTTCAGTATGTGTAACCTAA

Downstream 100 bases:

>100_bases
ACCTTGCTTCTTGCTTCACACTTACCGGAGTGCTATTAGTGTGACTCATGGAAGAGTTAGGTGTGTATTCTTTTTTAGCT
TTATCTGTTAATTCATATTC

Product: poly A polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQIDHETSVIIEAIEKFGGEARLVGGCVRDSILQRDIHDIDLATNLLPNQAVKALKLRNIKTIPTGLKHGTITAILNKRS
FEITTLRHDVKCDGRHAKVEFTNDWQADASRRDFTFNALYADKYGHIYDYFGGIQDLKARKLNFIGNAEDRIKEDYLRIL
RAFRFHAKICIGDLSDEILDVCKKHSHMIQNLSGERIREEIFKLLECNDPAPTLKSMQKSDVLQKIIPKEVKCEILSSPL
LINTKPPTKPSAYGHTPFLDPTIDALTKLALLIRTAEDRVSLGEEVSKFLRLSNKQKKKLLFLLSNDIKTELSEKEQKKY
ISLFGKELYCDLMKICGIESGTNVDEYISFAHIFNIPKFPLSGDDLIIIGHQPGKNLGRNLELLRQHWEDSSYTLTKEEL
LLYAKSFI

Sequences:

>Translated_408_residues
MQIDHETSVIIEAIEKFGGEARLVGGCVRDSILQRDIHDIDLATNLLPNQAVKALKLRNIKTIPTGLKHGTITAILNKRS
FEITTLRHDVKCDGRHAKVEFTNDWQADASRRDFTFNALYADKYGHIYDYFGGIQDLKARKLNFIGNAEDRIKEDYLRIL
RAFRFHAKICIGDLSDEILDVCKKHSHMIQNLSGERIREEIFKLLECNDPAPTLKSMQKSDVLQKIIPKEVKCEILSSPL
LINTKPPTKPSAYGHTPFLDPTIDALTKLALLIRTAEDRVSLGEEVSKFLRLSNKQKKKLLFLLSNDIKTELSEKEQKKY
ISLFGKELYCDLMKICGIESGTNVDEYISFAHIFNIPKFPLSGDDLIIIGHQPGKNLGRNLELLRQHWEDSSYTLTKEEL
LLYAKSFI
>Mature_408_residues
MQIDHETSVIIEAIEKFGGEARLVGGCVRDSILQRDIHDIDLATNLLPNQAVKALKLRNIKTIPTGLKHGTITAILNKRS
FEITTLRHDVKCDGRHAKVEFTNDWQADASRRDFTFNALYADKYGHIYDYFGGIQDLKARKLNFIGNAEDRIKEDYLRIL
RAFRFHAKICIGDLSDEILDVCKKHSHMIQNLSGERIREEIFKLLECNDPAPTLKSMQKSDVLQKIIPKEVKCEILSSPL
LINTKPPTKPSAYGHTPFLDPTIDALTKLALLIRTAEDRVSLGEEVSKFLRLSNKQKKKLLFLLSNDIKTELSEKEQKKY
ISLFGKELYCDLMKICGIESGTNVDEYISFAHIFNIPKFPLSGDDLIIIGHQPGKNLGRNLELLRQHWEDSSYTLTKEEL
LLYAKSFI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=410, Percent_Identity=35.1219512195122, Blast_Score=204, Evalue=1e-52,
Organism=Escherichia coli, GI87081691, Length=232, Percent_Identity=31.0344827586207, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71995920, Length=404, Percent_Identity=33.9108910891089, Blast_Score=188, Evalue=4e-48,
Organism=Drosophila melanogaster, GI281360112, Length=412, Percent_Identity=33.252427184466, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI21357337, Length=412, Percent_Identity=33.252427184466, Blast_Score=184, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46555; Mature: 46555

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIDHETSVIIEAIEKFGGEARLVGGCVRDSILQRDIHDIDLATNLLPNQAVKALKLRNI
CCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
KTIPTGLKHGTITAILNKRSFEITTLRHDVKCDGRHAKVEFTNDWQADASRRDFTFNALY
CCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEEEEE
ADKYGHIYDYFGGIQDLKARKLNFIGNAEDRIKEDYLRILRAFRFHAKICIGDLSDEILD
ECCCCCHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHH
VCKKHSHMIQNLSGERIREEIFKLLECNDPAPTLKSMQKSDVLQKIIPKEVKCEILSSPL
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCE
LINTKPPTKPSAYGHTPFLDPTIDALTKLALLIRTAEDRVSLGEEVSKFLRLSNKQKKKL
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHE
LFLLSNDIKTELSEKEQKKYISLFGKELYCDLMKICGIESGTNVDEYISFAHIFNIPKFP
EEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
LSGDDLIIIGHQPGKNLGRNLELLRQHWEDSSYTLTKEELLLYAKSFI
CCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHCC
>Mature Secondary Structure
MQIDHETSVIIEAIEKFGGEARLVGGCVRDSILQRDIHDIDLATNLLPNQAVKALKLRNI
CCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
KTIPTGLKHGTITAILNKRSFEITTLRHDVKCDGRHAKVEFTNDWQADASRRDFTFNALY
CCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEEEEE
ADKYGHIYDYFGGIQDLKARKLNFIGNAEDRIKEDYLRILRAFRFHAKICIGDLSDEILD
ECCCCCHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHH
VCKKHSHMIQNLSGERIREEIFKLLECNDPAPTLKSMQKSDVLQKIIPKEVKCEILSSPL
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCE
LINTKPPTKPSAYGHTPFLDPTIDALTKLALLIRTAEDRVSLGEEVSKFLRLSNKQKKKL
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHE
LFLLSNDIKTELSEKEQKKYISLFGKELYCDLMKICGIESGTNVDEYISFAHIFNIPKFP
EEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCC
LSGDDLIIIGHQPGKNLGRNLELLRQHWEDSSYTLTKEELLLYAKSFI
CCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA