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The map label for this gene is prfB [H]

Identifier: 189499218

GI number: 189499218

Start: 226660

End: 227751

Strand: Reverse

Name: prfB [H]

Synonym: Cphamn1_0238

Alternate gene names: 189499218

Gene position: 227751-226660 (Counterclockwise)

Preceding gene: 189499223

Following gene: 189499217

Centisome position: 8.32

GC content: 51.1

Gene sequence:

>1092_bases
TTGAAGGAGCAAAAGAGCGTCTGCACGAGATACACCACCGCATTGAACAGTTACGGGGGTATCTTTGACGTTGACGAACG
TTTGCACAAAATAGGTGAACTCGAGCAGATCACCACAGAAGAAAACTTCTGGGAGAACCAGCAGGAAGCCAACAAGATCG
TAAAAGAGATCAACGACCATAAACTATGGACTGAAGGATTTCTCGCGCTTGAAAAACAAACCGCTGACCTCAGGGAAGAA
CTGGAAATCGCTGAAGAACTCGGGGAAGAGTCACTTGCGGAAGAAATAGACTCGCGCATCGGTTCGATCGAGCAGAAAAT
CGCTGAGATCGAATTCAAAAACATGCTCTCCGGTAAAGACGATCCCGGCAACGCACTGCTCACTATCAATGCCGGTGCTG
GCGGCACCGAAGCACAGGACTGGGCGGAGATGCTCTACCGCATGTACCTGCGCTGGGCGGAACGTAAAGGGTTCAAGATT
TTCACCGATGACTATCAGGAAGGCGATGGCGCAGGCATAAAAACCGCGACGCTTGAGATACAGGGGCCCTATGCATACGG
CTACCTGAAAGCTGAAAACGGCGTCCATCGGCTGGTAAGGGTCTCTCCTTTCGATTCGAACGCTCGCCGCCACACCTCGT
TTGCCAGTGTCTACTCCTACCCGGAAGCGCCGCCCGATGCTGAAATAGAGATTCGCAAGGAGGACCTTGAACTTTCCACC
TTCCGCAGCGGCGGCAAAGGCGGTCAGAACGTCAACAAGGTTGAAACAGCCGTACGCATCAAGCATATCCCTTCCGGGAT
TGTCGTCTCCTGCCAACAGGAGCGTTCGCAGTTCCAGAACCGTGAACGGGCGACAAAAATGCTGCGGGCACAGCTTTACA
AAAAGCAGCGTGAAGAGGAAGAGGCAAGAAAACAGGCCGTTGAGGGCACGAAGAAAAAAATCGAATGGGGTAGCCAGATC
CGCAGCTATGTCATGGATGACAGAAGGATCAAGGACCACAGAACAAATTATGAACGACACGACATCGAAAATGTCCTTGA
CGGCGATATCGACGATTTTATAGAAAAATATCTTTCGGAATTCACCAACTGA

Upstream 100 bases:

>100_bases
AATTGATTTTTTGTTTCACTCATCAAGTAATCCATGAAACCTGCAAAACAGCGCGGTTTAAAAACATTTCACAACGAGAG
GAAGAAAGGAAATACCATGT

Downstream 100 bases:

>100_bases
CTCCAGAAACCAATCACATAACGCTATTAACGATTCACAACCGACACCTATACGGTCCAGGAGACCGAGCAAACGTGCTG
TTGTAGACGGCACCTTGCGA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKEQKSVCTRYTTALNSYGGIFDVDERLHKIGELEQITTEENFWENQQEANKIVKEINDHKLWTEGFLALEKQTADLREE
LEIAEELGEESLAEEIDSRIGSIEQKIAEIEFKNMLSGKDDPGNALLTINAGAGGTEAQDWAEMLYRMYLRWAERKGFKI
FTDDYQEGDGAGIKTATLEIQGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYSYPEAPPDAEIEIRKEDLELST
FRSGGKGGQNVNKVETAVRIKHIPSGIVVSCQQERSQFQNRERATKMLRAQLYKKQREEEEARKQAVEGTKKKIEWGSQI
RSYVMDDRRIKDHRTNYERHDIENVLDGDIDDFIEKYLSEFTN

Sequences:

>Translated_363_residues
MKEQKSVCTRYTTALNSYGGIFDVDERLHKIGELEQITTEENFWENQQEANKIVKEINDHKLWTEGFLALEKQTADLREE
LEIAEELGEESLAEEIDSRIGSIEQKIAEIEFKNMLSGKDDPGNALLTINAGAGGTEAQDWAEMLYRMYLRWAERKGFKI
FTDDYQEGDGAGIKTATLEIQGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYSYPEAPPDAEIEIRKEDLELST
FRSGGKGGQNVNKVETAVRIKHIPSGIVVSCQQERSQFQNRERATKMLRAQLYKKQREEEEARKQAVEGTKKKIEWGSQI
RSYVMDDRRIKDHRTNYERHDIENVLDGDIDDFIEKYLSEFTN
>Mature_363_residues
MKEQKSVCTRYTTALNSYGGIFDVDERLHKIGELEQITTEENFWENQQEANKIVKEINDHKLWTEGFLALEKQTADLREE
LEIAEELGEESLAEEIDSRIGSIEQKIAEIEFKNMLSGKDDPGNALLTINAGAGGTEAQDWAEMLYRMYLRWAERKGFKI
FTDDYQEGDGAGIKTATLEIQGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYSYPEAPPDAEIEIRKEDLELST
FRSGGKGGQNVNKVETAVRIKHIPSGIVVSCQQERSQFQNRERATKMLRAQLYKKQREEEEARKQAVEGTKKKIEWGSQI
RSYVMDDRRIKDHRTNYERHDIENVLDGDIDDFIEKYLSEFTN

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=35.0180505415162, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI34577120, Length=273, Percent_Identity=35.8974358974359, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI166795305, Length=180, Percent_Identity=32.2222222222222, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=46.0176991150442, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI1787462, Length=247, Percent_Identity=40.080971659919, Blast_Score=171, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17542784, Length=264, Percent_Identity=32.5757575757576, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=247, Percent_Identity=35.2226720647773, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19921226, Length=257, Percent_Identity=38.9105058365759, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41783; Mature: 41783

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEQKSVCTRYTTALNSYGGIFDVDERLHKIGELEQITTEENFWENQQEANKIVKEINDH
CCCHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHCC
KLWTEGFLALEKQTADLREELEIAEELGEESLAEEIDSRIGSIEQKIAEIEFKNMLSGKD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DPGNALLTINAGAGGTEAQDWAEMLYRMYLRWAERKGFKIFTDDYQEGDGAGIKTATLEI
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEE
QGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYSYPEAPPDAEIEIRKEDLELST
ECCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHH
FRSGGKGGQNVNKVETAVRIKHIPSGIVVSCQQERSQFQNRERATKMLRAQLYKKQREEE
HHCCCCCCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EARKQAVEGTKKKIEWGSQIRSYVMDDRRIKDHRTNYERHDIENVLDGDIDDFIEKYLSE
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHH
FTN
HCC
>Mature Secondary Structure
MKEQKSVCTRYTTALNSYGGIFDVDERLHKIGELEQITTEENFWENQQEANKIVKEINDH
CCCHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHCC
KLWTEGFLALEKQTADLREELEIAEELGEESLAEEIDSRIGSIEQKIAEIEFKNMLSGKD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DPGNALLTINAGAGGTEAQDWAEMLYRMYLRWAERKGFKIFTDDYQEGDGAGIKTATLEI
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEE
QGPYAYGYLKAENGVHRLVRVSPFDSNARRHTSFASVYSYPEAPPDAEIEIRKEDLELST
ECCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHH
FRSGGKGGQNVNKVETAVRIKHIPSGIVVSCQQERSQFQNRERATKMLRAQLYKKQREEE
HHCCCCCCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EARKQAVEGTKKKIEWGSQIRSYVMDDRRIKDHRTNYERHDIENVLDGDIDDFIEKYLSE
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHH
FTN
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]