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The map label for this gene is amt [H]

Identifier: 189499101

GI number: 189499101

Start: 100610

End: 101944

Strand: Direct

Name: amt [H]

Synonym: Cphamn1_0107

Alternate gene names: 189499101

Gene position: 100610-101944 (Clockwise)

Preceding gene: 189499099

Following gene: 189499102

Centisome position: 3.68

GC content: 51.54

Gene sequence:

>1335_bases
ATGGTTATGGGTAAAAAGCTGATCAGTACCGGATTGTTATTGCTGCTGTTTATGATCGTTGCTGATACGGTCTGGGCTTC
AGACGGGGTCACAGATTCCGGCACAACGGCATGGATGCTGACCGCAACGACTCTTGTTCTGCTCATGGTGCCGGGGCTGG
CAATGTTCTATGGGGGGCTCGTGCGCACAAAAAATGTTCTCGGCACCATGATGCACAGTTTTGCTGCAATGGTTGTTATC
GGGGTATTATGGCCCTTTCTGGGGTATGCATTGAGCTTCGGGCCAAGTGTATTGGGCGGCCTTGTCGGGTGGAATCCTGA
CTATGTGCTGCTTCGAGGAATTGACGATTCAATTCTGGATTCGGGTATTCCTGAATATGTGTTTGCCATGTTTCAGGGGA
AATTCGCTATCATCGCGCCTGCCCTGATAGCTGGTGCTTTTGCTGAAAGAGTCAGCTTCAGAGGGTATGTCGTGTTTATT
GCGCTCTGGAGTCTCGTGGTGTATAGCCCGATCTGTCACTGGGTTTGGGCTGAAGATGGTTTTCTGTTCAATATGGGGCC
GGCAGGGGCTATAGATTTTGCGGGAGGTACCGTCGTGCATATCTCTTCCGGTATCAGCGCTCTTGTGGCCGCACTGTTTC
TCGGTTCAAGGCGTGGATATCCGAAAAATGTCATGCATCCCAACAATCTGGTTATGACGATGATTGGTGCCGGATTGCTC
TGGGTAGGGTGGTTTGGATTCAACGCGGGCAGCGCTGTTGCCAGTGATCTCGATACAGGAAGGGCGCTTACCGTCACACA
GATTGCGGCGGCAGCTGGAGCGGGAGCCTGGATGATCATAGAGTCGCTGCTGTACAAAAAAATAACGACTCTCGGGGTTG
TGTCGGGTATTCTTGCAGGTCTTGTTGCGATAACCCCTGCAGCAGGTGTCGTTCAGCCTGGTGGCGCAATGGTGATCGGT
ATTCTCGCGTCTGTGTTCTGTTTTCTGGCCATTCAGGTGAAAAACCGTGCAGGATATGATGACAGTCTCGACGCGTTCGG
TATTCATGGGATCGGTGGTATTGTGGGTGCTCTCGCACTCACCTTTTTCATTCGTGAAGCCTGGATGGCTGATGCAGCGG
TGGCCGCGGGAGGCAGCTGGACTGTCTGGCAGCAGTTTGGTGTTCAGGCAGCTGCAGTGGTGATTACGATCGTCTACGCG
GCTATTGTCACGATCATTCTGCTCGTTATTATTGAAAAAACCATTGGTTTCCGCTTGAAAGAGACTGATGAAATGACCGG
ACTTGATCAGAGCCTGCATGGGGAGCACGGTTATGGTCTTATCAACCTTAATTAA

Upstream 100 bases:

>100_bases
TAATTATTTATAGTTTATCGTTTGTTAGACTTAAAATTATTTTTAATTTAGTCTCGTTGTTATTAAAAAATAAGGATTAT
AGAAATATCTGTTAAACGTA

Downstream 100 bases:

>100_bases
TTTTCAATACTATGAAGCTGATAACAGCCATAATTCAGGAGGACAAGCTGGATACCGTTCGTGAGGCATTGATCCAGTCG
GATATTTCCAGGATATCAGT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MVMGKKLISTGLLLLLFMIVADTVWASDGVTDSGTTAWMLTATTLVLLMVPGLAMFYGGLVRTKNVLGTMMHSFAAMVVI
GVLWPFLGYALSFGPSVLGGLVGWNPDYVLLRGIDDSILDSGIPEYVFAMFQGKFAIIAPALIAGAFAERVSFRGYVVFI
ALWSLVVYSPICHWVWAEDGFLFNMGPAGAIDFAGGTVVHISSGISALVAALFLGSRRGYPKNVMHPNNLVMTMIGAGLL
WVGWFGFNAGSAVASDLDTGRALTVTQIAAAAGAGAWMIIESLLYKKITTLGVVSGILAGLVAITPAAGVVQPGGAMVIG
ILASVFCFLAIQVKNRAGYDDSLDAFGIHGIGGIVGALALTFFIREAWMADAAVAAGGSWTVWQQFGVQAAAVVITIVYA
AIVTIILLVIIEKTIGFRLKETDEMTGLDQSLHGEHGYGLINLN

Sequences:

>Translated_444_residues
MVMGKKLISTGLLLLLFMIVADTVWASDGVTDSGTTAWMLTATTLVLLMVPGLAMFYGGLVRTKNVLGTMMHSFAAMVVI
GVLWPFLGYALSFGPSVLGGLVGWNPDYVLLRGIDDSILDSGIPEYVFAMFQGKFAIIAPALIAGAFAERVSFRGYVVFI
ALWSLVVYSPICHWVWAEDGFLFNMGPAGAIDFAGGTVVHISSGISALVAALFLGSRRGYPKNVMHPNNLVMTMIGAGLL
WVGWFGFNAGSAVASDLDTGRALTVTQIAAAAGAGAWMIIESLLYKKITTLGVVSGILAGLVAITPAAGVVQPGGAMVIG
ILASVFCFLAIQVKNRAGYDDSLDAFGIHGIGGIVGALALTFFIREAWMADAAVAAGGSWTVWQQFGVQAAAVVITIVYA
AIVTIILLVIIEKTIGFRLKETDEMTGLDQSLHGEHGYGLINLN
>Mature_444_residues
MVMGKKLISTGLLLLLFMIVADTVWASDGVTDSGTTAWMLTATTLVLLMVPGLAMFYGGLVRTKNVLGTMMHSFAAMVVI
GVLWPFLGYALSFGPSVLGGLVGWNPDYVLLRGIDDSILDSGIPEYVFAMFQGKFAIIAPALIAGAFAERVSFRGYVVFI
ALWSLVVYSPICHWVWAEDGFLFNMGPAGAIDFAGGTVVHISSGISALVAALFLGSRRGYPKNVMHPNNLVMTMIGAGLL
WVGWFGFNAGSAVASDLDTGRALTVTQIAAAAGAGAWMIIESLLYKKITTLGVVSGILAGLVAITPAAGVVQPGGAMVIG
ILASVFCFLAIQVKNRAGYDDSLDAFGIHGIGGIVGALALTFFIREAWMADAAVAAGGSWTVWQQFGVQAAAVVITIVYA
AIVTIILLVIIEKTIGFRLKETDEMTGLDQSLHGEHGYGLINLN

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=433, Percent_Identity=38.3371824480369, Blast_Score=261, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI32564042, Length=435, Percent_Identity=28.9655172413793, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI133931100, Length=433, Percent_Identity=27.7136258660508, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17550180, Length=445, Percent_Identity=26.5168539325843, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17550182, Length=442, Percent_Identity=24.4343891402715, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6325396, Length=420, Percent_Identity=34.0476190476191, Blast_Score=227, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=35.3488372093023, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321559, Length=420, Percent_Identity=31.6666666666667, Blast_Score=207, Evalue=3e-54,
Organism=Drosophila melanogaster, GI161078314, Length=415, Percent_Identity=30.6024096385542, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46699; Mature: 46699

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMGKKLISTGLLLLLFMIVADTVWASDGVTDSGTTAWMLTATTLVLLMVPGLAMFYGGL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VRTKNVLGTMMHSFAAMVVIGVLWPFLGYALSFGPSVLGGLVGWNPDYVLLRGIDDSILD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEECCCHHHHH
SGIPEYVFAMFQGKFAIIAPALIAGAFAERVSFRGYVVFIALWSLVVYSPICHWVWAEDG
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECCCC
FLFNMGPAGAIDFAGGTVVHISSGISALVAALFLGSRRGYPKNVMHPNNLVMTMIGAGLL
EEEECCCCCCEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
WVGWFGFNAGSAVASDLDTGRALTVTQIAAAAGAGAWMIIESLLYKKITTLGVVSGILAG
HHHHHCCCCCHHHHHHCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVAITPAAGVVQPGGAMVIGILASVFCFLAIQVKNRAGYDDSLDAFGIHGIGGIVGALAL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
TFFIREAWMADAAVAAGGSWTVWQQFGVQAAAVVITIVYAAIVTIILLVIIEKTIGFRLK
HHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEC
ETDEMTGLDQSLHGEHGYGLINLN
CCHHHCCCCHHCCCCCCEEEEEEC
>Mature Secondary Structure
MVMGKKLISTGLLLLLFMIVADTVWASDGVTDSGTTAWMLTATTLVLLMVPGLAMFYGGL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VRTKNVLGTMMHSFAAMVVIGVLWPFLGYALSFGPSVLGGLVGWNPDYVLLRGIDDSILD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEECCCHHHHH
SGIPEYVFAMFQGKFAIIAPALIAGAFAERVSFRGYVVFIALWSLVVYSPICHWVWAEDG
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECCCC
FLFNMGPAGAIDFAGGTVVHISSGISALVAALFLGSRRGYPKNVMHPNNLVMTMIGAGLL
EEEECCCCCCEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
WVGWFGFNAGSAVASDLDTGRALTVTQIAAAAGAGAWMIIESLLYKKITTLGVVSGILAG
HHHHHCCCCCHHHHHHCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVAITPAAGVVQPGGAMVIGILASVFCFLAIQVKNRAGYDDSLDAFGIHGIGGIVGALAL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
TFFIREAWMADAAVAAGGSWTVWQQFGVQAAAVVITIVYAAIVTIILLVIIEKTIGFRLK
HHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEC
ETDEMTGLDQSLHGEHGYGLINLN
CCHHHCCCCHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]