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The map label for this gene is prfA [H]

Identifier: 189499090

GI number: 189499090

Start: 88279

End: 89352

Strand: Reverse

Name: prfA [H]

Synonym: Cphamn1_0096

Alternate gene names: 189499090

Gene position: 89352-88279 (Counterclockwise)

Preceding gene: 189499091

Following gene: 189499089

Centisome position: 3.27

GC content: 51.3

Gene sequence:

>1074_bases
ATGCTTGAAAAACTTGAAGCGTTAAAGGACAAATACCTTCGGCTTGAAGAACAGCTTTCCGATCCGGAAATCATTGCCGA
CCAGAAGCGTTTCAGAAAGCTCAACAAGGAATACAGCGACCTGAAAGAAATTGTTCAGGGCTATGATCGCTACAAGTCCA
ACAAGGTGCAGATCGAAGAGGTACGCAAGATGCTCAAACAGGAAACAGATCCTGAAATGAAGTCTCTTGCACTGGTCGAA
CTTGAAGAGCTTCAGGAGCTTCAGCCGGAACTCGATCAGCAGTTGAAGCTGCTGCTGTTGCCAAAGGAGGAAGCTGACTC
GAGAAACGTCATCATTGAGATCCGGGCAGGAACCGGGGGAGATGAAGCCGCGCTGTTCGCAACGGACCTCTTGCGAATGT
ACCAGAAATTTGCCGAACGCAAAGGATGGAAATGCGACATGATGGAGTTTAACGAAGCGAGCATTCCGGGAGCATGCAAA
GAAGCCATACTGAGTATCAGCGGTCACGACGTCTATGCAACCATGAAGTTTGAAAGTGGTGTTCACCGTGTACAGAGAGT
GCCTGAAACAGAAACGCAGGGAAGAATCCACACATCGGCGGCCAGTGTCGCCGTGCTCCCGGAAGCTGAAGATGTCGATA
TAGAGATCCGCAAGGATGACCTTCAACTCGACACTTTCCGAAGCGGTGGAAAAGGGGGGCAGAACGTCAACAAGGTCGAG
ACCGCGGTCAGAATAACCCATACCCCGTCAGGTATTGTCGTAGCCTGTCAGGAAGAACGTTCTCAGCTTCAGAACCGCGA
ACGGGCGATGAAAATGCTGCGGGCCAAACTCTACGACCAGCAGCTTGCCGAAAAAAACAGGGAACGCGCGGATCTGCGAA
GATCAATGGTCACGACCGGCGACCGCAGCGCTAAAATCCGTACTTACAATTTCCCTCAGTCGCGAGTAACCGACCATCGC
ATAGGCTATACCAGCCATGCGCTTCCGCAGATACTCGAAGGGAATCTGGACGACATCATTGAAGCACTCAAGCTTCATGA
CCAAACTGCCCGCCTTCAGGCAGAAGCCATTTAG

Upstream 100 bases:

>100_bases
AACAGGTCGGCATGGCTCTGCTTCTGACGCTCTTTTTATTCATTATCATCAATGATATTATCAACCCCTGATGAAGAGGG
GAACGGAGTAAAGGCAGACT

Downstream 100 bases:

>100_bases
CCGGCTTAAATCATCATACGGCCAGCACAGATACAGTATGATCAAAAACATAAAATGTTAGTATAGAGTATGGAAGCACA
CGGGCATACTAAAGGAGAAT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLEKLEALKDKYLRLEEQLSDPEIIADQKRFRKLNKEYSDLKEIVQGYDRYKSNKVQIEEVRKMLKQETDPEMKSLALVE
LEELQELQPELDQQLKLLLLPKEEADSRNVIIEIRAGTGGDEAALFATDLLRMYQKFAERKGWKCDMMEFNEASIPGACK
EAILSISGHDVYATMKFESGVHRVQRVPETETQGRIHTSAASVAVLPEAEDVDIEIRKDDLQLDTFRSGGKGGQNVNKVE
TAVRITHTPSGIVVACQEERSQLQNRERAMKMLRAKLYDQQLAEKNRERADLRRSMVTTGDRSAKIRTYNFPQSRVTDHR
IGYTSHALPQILEGNLDDIIEALKLHDQTARLQAEAI

Sequences:

>Translated_357_residues
MLEKLEALKDKYLRLEEQLSDPEIIADQKRFRKLNKEYSDLKEIVQGYDRYKSNKVQIEEVRKMLKQETDPEMKSLALVE
LEELQELQPELDQQLKLLLLPKEEADSRNVIIEIRAGTGGDEAALFATDLLRMYQKFAERKGWKCDMMEFNEASIPGACK
EAILSISGHDVYATMKFESGVHRVQRVPETETQGRIHTSAASVAVLPEAEDVDIEIRKDDLQLDTFRSGGKGGQNVNKVE
TAVRITHTPSGIVVACQEERSQLQNRERAMKMLRAKLYDQQLAEKNRERADLRRSMVTTGDRSAKIRTYNFPQSRVTDHR
IGYTSHALPQILEGNLDDIIEALKLHDQTARLQAEAI
>Mature_357_residues
MLEKLEALKDKYLRLEEQLSDPEIIADQKRFRKLNKEYSDLKEIVQGYDRYKSNKVQIEEVRKMLKQETDPEMKSLALVE
LEELQELQPELDQQLKLLLLPKEEADSRNVIIEIRAGTGGDEAALFATDLLRMYQKFAERKGWKCDMMEFNEASIPGACK
EAILSISGHDVYATMKFESGVHRVQRVPETETQGRIHTSAASVAVLPEAEDVDIEIRKDDLQLDTFRSGGKGGQNVNKVE
TAVRITHTPSGIVVACQEERSQLQNRERAMKMLRAKLYDQQLAEKNRERADLRRSMVTTGDRSAKIRTYNFPQSRVTDHR
IGYTSHALPQILEGNLDDIIEALKLHDQTARLQAEAI

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=290, Percent_Identity=45.1724137931035, Blast_Score=263, Evalue=1e-70,
Organism=Homo sapiens, GI34577120, Length=353, Percent_Identity=38.5269121813031, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI166795305, Length=194, Percent_Identity=42.2680412371134, Blast_Score=168, Evalue=8e-42,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=48.169014084507, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI2367172, Length=333, Percent_Identity=37.2372372372372, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=284, Percent_Identity=41.5492957746479, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=325, Percent_Identity=40, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI19921226, Length=375, Percent_Identity=38.4, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40853; Mature: 40853

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50222 EF_HAND_2 ; PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKLEALKDKYLRLEEQLSDPEIIADQKRFRKLNKEYSDLKEIVQGYDRYKSNKVQIEE
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHH
VRKMLKQETDPEMKSLALVELEELQELQPELDQQLKLLLLPKEEADSRNVIIEIRAGTGG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECCCCC
DEAALFATDLLRMYQKFAERKGWKCDMMEFNEASIPGACKEAILSISGHDVYATMKFESG
CHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEHHHH
VHRVQRVPETETQGRIHTSAASVAVLPEAEDVDIEIRKDDLQLDTFRSGGKGGQNVNKVE
HHHHHCCCCCCCCCEEEECCCCEEECCCCCCCEEEEECCCCEEHHHCCCCCCCCCHHHHH
TAVRITHTPSGIVVACQEERSQLQNRERAMKMLRAKLYDQQLAEKNRERADLRRSMVTTG
HEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRSAKIRTYNFPQSRVTDHRIGYTSHALPQILEGNLDDIIEALKLHDQTARLQAEAI
CCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MLEKLEALKDKYLRLEEQLSDPEIIADQKRFRKLNKEYSDLKEIVQGYDRYKSNKVQIEE
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHH
VRKMLKQETDPEMKSLALVELEELQELQPELDQQLKLLLLPKEEADSRNVIIEIRAGTGG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECCCCC
DEAALFATDLLRMYQKFAERKGWKCDMMEFNEASIPGACKEAILSISGHDVYATMKFESG
CHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEHHHH
VHRVQRVPETETQGRIHTSAASVAVLPEAEDVDIEIRKDDLQLDTFRSGGKGGQNVNKVE
HHHHHCCCCCCCCCEEEECCCCEEECCCCCCCEEEEECCCCEEHHHCCCCCCCCCHHHHH
TAVRITHTPSGIVVACQEERSQLQNRERAMKMLRAKLYDQQLAEKNRERADLRRSMVTTG
HEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DRSAKIRTYNFPQSRVTDHRIGYTSHALPQILEGNLDDIIEALKLHDQTARLQAEAI
CCCCEEEEECCCHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA