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The map label for this gene is ykfA [H]

Identifier: 189499029

GI number: 189499029

Start: 34128

End: 35078

Strand: Reverse

Name: ykfA [H]

Synonym: Cphamn1_0029

Alternate gene names: 189499029

Gene position: 35078-34128 (Counterclockwise)

Preceding gene: 189499030

Following gene: 189499028

Centisome position: 1.28

GC content: 52.37

Gene sequence:

>951_bases
ATGAGAACCCTGATTCCCAAAGCACTCCGGCATGGTGACACCATCGGGCTCATCTCACCATCGTCTCCTTCTCCCGACCA
CGAAAAAGTCGGTCACGCGGTCTCTTATCTTGAAAAACTCGGCTACCGGGTAAAGCCTGCTGAGCACTTCAACTCCAGAA
CAGAAAACCGCCTGGAGCTTGACCGGCGAAAACTCAGCGACCTCCATGAGATGTTTGCCGATACATCCGTGAAGGCGATT
TTCTGCCTGAGAGGAGGATCAGGAGCCACAAGACTTCTGCAGGATCTCGATTACGAGCTGGTCTCACGAAACCCGAAAAT
CCTCGCAGGGTATTCTGATATCACAGCTCTCTCGATAGGGCTCTTCGCCAAAACCGGCCTGCTGACCTTTTCAGGCCCGA
TGCTTGCGACAGAACTTCACAACCCCAGCGGTTACACAGAAGAGAATTTCTGGAAAATACTTGAATCGACATCGTCTGTT
CACCAGATCATCAACCATCCTGACCATCCCATTAACGTCTACAGGGAAGGCTCTGCCACAGGAACGCTGCTGGCAGGCAA
TCTGACTGTTTTATCCTGCCTGGTAGGCACCCCCTACCTGCCTCCCCTGGATGGCACTCTGCCGATTTTTGAGGATATCA
ATGAAGAGCCTTACAGGATCGACCGGCTCCTTTCCCACTTCCACAATGCAGGTCTTCTCAGCAGCTGCTCAGGCATTCTG
TTCGGCCAATTCAGCAAAGAGATCATAACATTTGATACGCAGCATCCTCTTCTTGATATTCTGCGCTACTACACAGAGAA
AACCTCTCCGAATGTTCCTGTTGTCGCCGGGCTCTCCTACGGACATATCGACGACCTGCTCACACTGCCCGTCGGTGCAA
CCTGCACGCTAAACGCCAGCCACGAAAGAATTTCATTGACGACGTCCCCCGCTGTATGCAGTGACCAGTGA

Upstream 100 bases:

>100_bases
CGGAAGACATTTCATAATCCGGACTACGGAACTAACGCGCTATAAAAAACATCAGTGACAGGAAAAATCTCCCAATCACT
GATGTTTTTTTCTTATACAC

Downstream 100 bases:

>100_bases
TTGTTTATGTGTCAGGTGGGGAGGAGGTGGTTCTGAGTTCTCAGTTCTGAGCGATGAGTGGGAAATGGGAAATGACTAGT
GACTAATGAATAATGAGTGG

Product: Muramoyltetrapeptide carboxypeptidase

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MRTLIPKALRHGDTIGLISPSSPSPDHEKVGHAVSYLEKLGYRVKPAEHFNSRTENRLELDRRKLSDLHEMFADTSVKAI
FCLRGGSGATRLLQDLDYELVSRNPKILAGYSDITALSIGLFAKTGLLTFSGPMLATELHNPSGYTEENFWKILESTSSV
HQIINHPDHPINVYREGSATGTLLAGNLTVLSCLVGTPYLPPLDGTLPIFEDINEEPYRIDRLLSHFHNAGLLSSCSGIL
FGQFSKEIITFDTQHPLLDILRYYTEKTSPNVPVVAGLSYGHIDDLLTLPVGATCTLNASHERISLTTSPAVCSDQ

Sequences:

>Translated_316_residues
MRTLIPKALRHGDTIGLISPSSPSPDHEKVGHAVSYLEKLGYRVKPAEHFNSRTENRLELDRRKLSDLHEMFADTSVKAI
FCLRGGSGATRLLQDLDYELVSRNPKILAGYSDITALSIGLFAKTGLLTFSGPMLATELHNPSGYTEENFWKILESTSSV
HQIINHPDHPINVYREGSATGTLLAGNLTVLSCLVGTPYLPPLDGTLPIFEDINEEPYRIDRLLSHFHNAGLLSSCSGIL
FGQFSKEIITFDTQHPLLDILRYYTEKTSPNVPVVAGLSYGHIDDLLTLPVGATCTLNASHERISLTTSPAVCSDQ
>Mature_316_residues
MRTLIPKALRHGDTIGLISPSSPSPDHEKVGHAVSYLEKLGYRVKPAEHFNSRTENRLELDRRKLSDLHEMFADTSVKAI
FCLRGGSGATRLLQDLDYELVSRNPKILAGYSDITALSIGLFAKTGLLTFSGPMLATELHNPSGYTEENFWKILESTSSV
HQIINHPDHPINVYREGSATGTLLAGNLTVLSCLVGTPYLPPLDGTLPIFEDINEEPYRIDRLLSHFHNAGLLSSCSGIL
FGQFSKEIITFDTQHPLLDILRYYTEKTSPNVPVVAGLSYGHIDDLLTLPVGATCTLNASHERISLTTSPAVCSDQ

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=304, Percent_Identity=32.8947368421053, Blast_Score=155, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 34724; Mature: 34724

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLIPKALRHGDTIGLISPSSPSPDHEKVGHAVSYLEKLGYRVKPAEHFNSRTENRLEL
CCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHH
DRRKLSDLHEMFADTSVKAIFCLRGGSGATRLLQDLDYELVSRNPKILAGYSDITALSIG
HHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCCHHHHCCCCCEEECCCHHHHHHHH
LFAKTGLLTFSGPMLATELHNPSGYTEENFWKILESTSSVHQIINHPDHPINVYREGSAT
HHHHCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
GTLLAGNLTVLSCLVGTPYLPPLDGTLPIFEDINEEPYRIDRLLSHFHNAGLLSSCSGIL
CEEEECHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHH
FGQFSKEIITFDTQHPLLDILRYYTEKTSPNVPVVAGLSYGHIDDLLTLPVGATCTLNAS
HHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHCCCCCEEEECCC
HERISLTTSPAVCSDQ
CCEEEEECCCCCCCCC
>Mature Secondary Structure
MRTLIPKALRHGDTIGLISPSSPSPDHEKVGHAVSYLEKLGYRVKPAEHFNSRTENRLEL
CCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHH
DRRKLSDLHEMFADTSVKAIFCLRGGSGATRLLQDLDYELVSRNPKILAGYSDITALSIG
HHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCCHHHHCCCCCEEECCCHHHHHHHH
LFAKTGLLTFSGPMLATELHNPSGYTEENFWKILESTSSVHQIINHPDHPINVYREGSAT
HHHHCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
GTLLAGNLTVLSCLVGTPYLPPLDGTLPIFEDINEEPYRIDRLLSHFHNAGLLSSCSGIL
CEEEECHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHH
FGQFSKEIITFDTQHPLLDILRYYTEKTSPNVPVVAGLSYGHIDDLLTLPVGATCTLNAS
HHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHCCCCCEEEECCC
HERISLTTSPAVCSDQ
CCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]