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The map label for this gene is garD [H]

Identifier: 189353867

GI number: 189353867

Start: 2172427

End: 2173983

Strand: Reverse

Name: garD [H]

Synonym: BMULJ_05119

Alternate gene names: 189353867

Gene position: 2173983-2172427 (Counterclockwise)

Preceding gene: 189353869

Following gene: 189353866

Centisome position: 87.9

GC content: 69.36

Gene sequence:

>1557_bases
ATGACTGCATCCCCGCTCTACATCCGTGTGCATCCGGACGACAACGTCGCGATCGTCGTCAACGACGGCGGTCTGCCGGA
GGGCGCGACGTTCGCCGACGGGCTGACGCTGCGCGAAGGCGTCCCGCAGGGCCACAAGGTCGCGCTGACCGATCTCGCGG
CCGGCGATCCGATCGTGCGCTACAACGTCGTGATCGGCTACGCGCTGACCGACCTGCGGCGCGGCAGCTGGGTCAACGAG
CGCACGATGCGCATGCCGGAGCCGCCGGGCCTCGACAACCTGCCGCTCGCGACGCGCCGCGCGCCGCCGCTGGCGCCGCT
CGAGGGCTATACGTTCGAGGGCTTCCGCAATCCGGACGGTTCGGTCGGGACGCGCAACATCCTCGCGATCACGACGACGG
TGCAGTGCGTGGCAGGCGTCGTCGAGCATGCGGTGAAGCGGATCAAGGCCGAACTGCTGCCGCGCTATCCGAACGTCGAC
GATGTCGTCGGGCTCGAGCACACGTACGGCTGCGGCGTCGCGATTGACGCGCCCGATGCCGACATCCCGATCCGCACGCT
GCGCAACATCAGCCTGAATCCGAACTTCGGCGGCGAAGTGATGACGGTCAGCCTCGGTTGCGAGAAGCTGCAGCCGGACC
GCCTGCTGCCGCCGGGCGCGATCCCGGTCGTCGCGGCCGAGGGCGAGCCCGACAACGGCGGCGTCGTGTGTCTGCAGGAT
GCGGCGCACGTCGGTTTCCAGTCGATGATCGATTCGATCATGACGATGGCCGAATCGCATCTCGAGCGGCTCAACCGCCG
GCGTCGCGAGACGTGTCCGGCGTCGGATCTCGTCGTCGGCGTGCAGTGCGGCGGCAGCGATGCGTTCTCGGGGCTGACGG
CCAATCCGGCGGTCGGCTTCGCGGCCGACCTGCTCGTGCGCGCCGGTGCGACGATCATGTTCTCCGAAGTGACCGAAGTG
CGCGACGGCGTCGCGCAGCTCACGTCGCGCGCGGCGAACGAGGACGTCGCGCGCGAGATCATCCGCGAGATGGACTGGTA
CGACCGCTATCTGCAGCGCGGCCGCGTCGACCGCAGCGCGAACACGACGCCGGGCAACAAGAAGGGCGGCCTGTCGAACA
TCGTCGAGAAGGCGATGGGCTCGATCGTGAAATCGGGCAGCGCGCCGATCGCCGGCGTCGTGCGACCGGGCGAGCGTGCC
CGGCAAAAGGGGCTGCTCTACACGGCGACGCCCGCGAGCGACTTCATCTGCGGCACGCTGCAGCTCGCGGCCGGCATGAA
CCTGCACGTGTTCACGACGGGCCGCGGCACGCCGTACGGGCTCGCGCAGGTGCCGGTGATCAAGGTCGCGACGCGCAGCG
ATCTCGCGCGCCGCTGGCACGACCTGATGGATCTCGACGCGGGGCGCATCGCGACCGGCGCGGCGACGATCGAGGACGTC
GGCTGGGAGCTGTTCCACATGATGCTCGACGTCGCGAGCGGCAAGCGCCGCACGTGGGCCGAGCAATGGAAGCTCGCGAA
TGCGCTCGTGCTGTTCAATCCGGCGCCGGTGACCTGA

Upstream 100 bases:

>100_bases
TAGTAGGGTCTTTCCGGATAAGGGTTTACGAACTATGCGCGATCGGTGTTCAATGTCGTATGATGACTAACGATATGACT
TGCCGCTTTCAGGAATTCCG

Downstream 100 bases:

>100_bases
CGCGCGGTTATCTCGGTCGAACGATGCGGGCGCCTTCGGGCGCCCGTTGTCGTTTTGGGGCGCCGCGTCGTGCGTCGCGC
GCCGCGTGCGCGCATCGCGA

Product: galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTASPLYIRVHPDDNVAIVVNDGGLPEGATFADGLTLREGVPQGHKVALTDLAAGDPIVRYNVVIGYALTDLRRGSWVNE
RTMRMPEPPGLDNLPLATRRAPPLAPLEGYTFEGFRNPDGSVGTRNILAITTTVQCVAGVVEHAVKRIKAELLPRYPNVD
DVVGLEHTYGCGVAIDAPDADIPIRTLRNISLNPNFGGEVMTVSLGCEKLQPDRLLPPGAIPVVAAEGEPDNGGVVCLQD
AAHVGFQSMIDSIMTMAESHLERLNRRRRETCPASDLVVGVQCGGSDAFSGLTANPAVGFAADLLVRAGATIMFSEVTEV
RDGVAQLTSRAANEDVAREIIREMDWYDRYLQRGRVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGSAPIAGVVRPGERA
RQKGLLYTATPASDFICGTLQLAAGMNLHVFTTGRGTPYGLAQVPVIKVATRSDLARRWHDLMDLDAGRIATGAATIEDV
GWELFHMMLDVASGKRRTWAEQWKLANALVLFNPAPVT

Sequences:

>Translated_518_residues
MTASPLYIRVHPDDNVAIVVNDGGLPEGATFADGLTLREGVPQGHKVALTDLAAGDPIVRYNVVIGYALTDLRRGSWVNE
RTMRMPEPPGLDNLPLATRRAPPLAPLEGYTFEGFRNPDGSVGTRNILAITTTVQCVAGVVEHAVKRIKAELLPRYPNVD
DVVGLEHTYGCGVAIDAPDADIPIRTLRNISLNPNFGGEVMTVSLGCEKLQPDRLLPPGAIPVVAAEGEPDNGGVVCLQD
AAHVGFQSMIDSIMTMAESHLERLNRRRRETCPASDLVVGVQCGGSDAFSGLTANPAVGFAADLLVRAGATIMFSEVTEV
RDGVAQLTSRAANEDVAREIIREMDWYDRYLQRGRVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGSAPIAGVVRPGERA
RQKGLLYTATPASDFICGTLQLAAGMNLHVFTTGRGTPYGLAQVPVIKVATRSDLARRWHDLMDLDAGRIATGAATIEDV
GWELFHMMLDVASGKRRTWAEQWKLANALVLFNPAPVT
>Mature_517_residues
TASPLYIRVHPDDNVAIVVNDGGLPEGATFADGLTLREGVPQGHKVALTDLAAGDPIVRYNVVIGYALTDLRRGSWVNER
TMRMPEPPGLDNLPLATRRAPPLAPLEGYTFEGFRNPDGSVGTRNILAITTTVQCVAGVVEHAVKRIKAELLPRYPNVDD
VVGLEHTYGCGVAIDAPDADIPIRTLRNISLNPNFGGEVMTVSLGCEKLQPDRLLPPGAIPVVAAEGEPDNGGVVCLQDA
AHVGFQSMIDSIMTMAESHLERLNRRRRETCPASDLVVGVQCGGSDAFSGLTANPAVGFAADLLVRAGATIMFSEVTEVR
DGVAQLTSRAANEDVAREIIREMDWYDRYLQRGRVDRSANTTPGNKKGGLSNIVEKAMGSIVKSGSAPIAGVVRPGERAR
QKGLLYTATPASDFICGTLQLAAGMNLHVFTTGRGTPYGLAQVPVIKVATRSDLARRWHDLMDLDAGRIATGAATIEDVG
WELFHMMLDVASGKRRTWAEQWKLANALVLFNPAPVT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=521, Percent_Identity=63.915547024952, Blast_Score=701, Evalue=0.0,
Organism=Escherichia coli, GI1789475, Length=506, Percent_Identity=31.8181818181818, Blast_Score=218, Evalue=9e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 55525; Mature: 55394

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASPLYIRVHPDDNVAIVVNDGGLPEGATFADGLTLREGVPQGHKVALTDLAAGDPIVR
CCCCCEEEEECCCCCEEEEECCCCCCCCCCHHCCCHHHCCCCCCCEEEEEECCCCCCEEE
YNVVIGYALTDLRRGSWVNERTMRMPEPPGLDNLPLATRRAPPLAPLEGYTFEGFRNPDG
EEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCC
SVGTRNILAITTTVQCVAGVVEHAVKRIKAELLPRYPNVDDVVGLEHTYGCGVAIDAPDA
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCC
DIPIRTLRNISLNPNFGGEVMTVSLGCEKLQPDRLLPPGAIPVVAAEGEPDNGGVVCLQD
CCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECC
AAHVGFQSMIDSIMTMAESHLERLNRRRRETCPASDLVVGVQCGGSDAFSGLTANPAVGF
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHCCCCCCCCCHHH
AADLLVRAGATIMFSEVTEVRDGVAQLTSRAANEDVAREIIREMDWYDRYLQRGRVDRSA
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
NTTPGNKKGGLSNIVEKAMGSIVKSGSAPIAGVVRPGERARQKGLLYTATPASDFICGTL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCEEEECCCCCHHHHHH
QLAAGMNLHVFTTGRGTPYGLAQVPVIKVATRSDLARRWHDLMDLDAGRIATGAATIEDV
HHHCCCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCEECCCHHHHHH
GWELFHMMLDVASGKRRTWAEQWKLANALVLFNPAPVT
HHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEECCCCCC
>Mature Secondary Structure 
TASPLYIRVHPDDNVAIVVNDGGLPEGATFADGLTLREGVPQGHKVALTDLAAGDPIVR
CCCCEEEEECCCCCEEEEECCCCCCCCCCHHCCCHHHCCCCCCCEEEEEECCCCCCEEE
YNVVIGYALTDLRRGSWVNERTMRMPEPPGLDNLPLATRRAPPLAPLEGYTFEGFRNPDG
EEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCC
SVGTRNILAITTTVQCVAGVVEHAVKRIKAELLPRYPNVDDVVGLEHTYGCGVAIDAPDA
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCC
DIPIRTLRNISLNPNFGGEVMTVSLGCEKLQPDRLLPPGAIPVVAAEGEPDNGGVVCLQD
CCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECC
AAHVGFQSMIDSIMTMAESHLERLNRRRRETCPASDLVVGVQCGGSDAFSGLTANPAVGF
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHCCCCCCCCCHHH
AADLLVRAGATIMFSEVTEVRDGVAQLTSRAANEDVAREIIREMDWYDRYLQRGRVDRSA
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
NTTPGNKKGGLSNIVEKAMGSIVKSGSAPIAGVVRPGERARQKGLLYTATPASDFICGTL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCEEEECCCCCHHHHHH
QLAAGMNLHVFTTGRGTPYGLAQVPVIKVATRSDLARRWHDLMDLDAGRIATGAATIEDV
HHHCCCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCEECCCHHHHHH
GWELFHMMLDVASGKRRTWAEQWKLANALVLFNPAPVT
HHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]