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The map label for this gene is ilvB [H]

Identifier: 189352345

GI number: 189352345

Start: 394179

End: 395819

Strand: Direct

Name: ilvB [H]

Synonym: BMULJ_03564

Alternate gene names: 189352345

Gene position: 394179-395819 (Clockwise)

Preceding gene: 189352342

Following gene: 189352346

Centisome position: 15.94

GC content: 64.96

Gene sequence:

>1641_bases
ATGAAAGCATCGGATCTGTTCGTGAAGGCGCTGGAGGCCGAGGGCGTCGAGTACGTGTTCGGCATCCCCGGCGAAGAAAA
CCTCGATCTGCTCGAATCGCTGCGGCGCTCGAAGATCCGGCTCGTGCTGACGCGGCACGAACAGGCGGCCGGCTTCATGG
CCGCGACCTACGGCCGCCTGACGGGCCGCACCGGCGTGTGTCTCGCGACGCTCGGGCCCGGCGCGACGAATTTCGTGACG
GCCGCCGCCTATGCGCAGCTCGGCGGGATGCCGATGCTGATGATCACGGGCCAGAAGCCGATCAAGTCCAGCAAGCAGGG
CCACTTCCAGATCGTCGACGTGGTCGGCATGATGCAGCCGCTGACGAAATTCACGCGGCAGATCGTGTCGATCGGCAATA
TTCCGTCGGCGGTGCGCGAGGCGTTCCGGCGCGCGGAGGAGGAGCGGCCGGGCGCCGCGCACCTCGAGCTGCCGGAAGAC
ATCGCGCACGAGGAAGGCGACGGCAAGCCGATCCCGCGCAGCTTCAGCCGGCGGCCGATCGCCGAGCAGAAGGCGGTCGC
GCACGCCGTCGACGCGATCCAGGCCGCGCGTCATCCGCTGCTGATGATCGGCGCGGGCGGCAACCGCAAGACGACCTGCA
AGATGCTGCTCGAATTCGTCGACAAGACGGGCATCCCGTTCTTCACGACGCAGATGGGCAAGGGCGTGATCGACGAGACG
CATCCGCTGTGGCTCGGCAATGCGACATTGTCCGACGGCGATTTCGTGCATCGCGCGATCGAGCATGCGGACTGCATCAT
CAACGTCGGCCACGACGTGATCGAGAAGCCGCCGTTCTTCATGCGCGCGGACGACAAGACCGTGATCCACGTGAACTTCC
TCGGCGCGCAGGTCGACCCCGTGTATTTCCCGCAGATCGAAGTGGTCGGCGACATCGCGAATGCGGTCTGGCAGATGAAG
GAGGCGATCGCGCCGCAGCCGCACTGGGATTTCGAGCGCTTCGCGATGATCAAGGAACATTTCGACGCGCACCTGCAGAA
GGGCCAGCACGATCCGCGCTTCCCGATGTATCCGGTGCGCATCGTCAACGATCTGTACAACGCGTTGCCGGTCGATGGCA
TCGTCTGTCTCGACAACGGGATGTACAAGATCTGGTTCGCGCGCTACTGGCGCGCGCACGAGCCGAACTCGCTGCTGCTC
GACAACGCGCTCGCGTCGATGGGCGCCGGTCTGCCGTCGGCGATCGCGACGAAGATCGTCCATCCGCAGCGCAAGGTGAT
CGCCGTGTGCGGCGACGGCGGCTTCATGATGAATTCGCAGGAGCTCGAGACGGCGGTGCGCCTCGGGCTCGACATCGTCG
TGATGATCCTGCGCGACGACGCGTTCGGCATGATCCGCTGGAAGCAGGAAAACATGAACTTCCCCGATTTCGCGATGACG
CTGAAGAATCCCGACTTCGTGTCGTATGCCCAGAGCTACGGCGCGCACGGCCATCGCGTCGAATCGGCCGATGCGCTCGA
GCCGCTGCTGCGCGAATGCTTCGCATCGCCGGGCGTGCACGTGATCGACGTGCCGGTCGACTATTCCGACAACGAGCGCG
TGCTGAACCGCGAGATCAAGCGGCTGTCGGCGCAGCTCTGA

Upstream 100 bases:

>100_bases
TATCCGGTAGGTAATACGTTCGCGCGCCGTAGGCCGGCGCGCGCCGTCTCAAGCGTCGCGCGTCCGTAAGCCGTGCGCGC
GGCATCAACCGAGAGTCCCC

Downstream 100 bases:

>100_bases
ATCCCCGTTCACCGGAAGGAGCCGTTCCATGCTGAAGGAAACCTATCCGTACTACCTGGCCAACGCGGCCGTCTACGCGA
ACACCGACCTCGAGGTCACG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKASDLFVKALEAEGVEYVFGIPGEENLDLLESLRRSKIRLVLTRHEQAAGFMAATYGRLTGRTGVCLATLGPGATNFVT
AAAYAQLGGMPMLMITGQKPIKSSKQGHFQIVDVVGMMQPLTKFTRQIVSIGNIPSAVREAFRRAEEERPGAAHLELPED
IAHEEGDGKPIPRSFSRRPIAEQKAVAHAVDAIQAARHPLLMIGAGGNRKTTCKMLLEFVDKTGIPFFTTQMGKGVIDET
HPLWLGNATLSDGDFVHRAIEHADCIINVGHDVIEKPPFFMRADDKTVIHVNFLGAQVDPVYFPQIEVVGDIANAVWQMK
EAIAPQPHWDFERFAMIKEHFDAHLQKGQHDPRFPMYPVRIVNDLYNALPVDGIVCLDNGMYKIWFARYWRAHEPNSLLL
DNALASMGAGLPSAIATKIVHPQRKVIAVCGDGGFMMNSQELETAVRLGLDIVVMILRDDAFGMIRWKQENMNFPDFAMT
LKNPDFVSYAQSYGAHGHRVESADALEPLLRECFASPGVHVIDVPVDYSDNERVLNREIKRLSAQL

Sequences:

>Translated_546_residues
MKASDLFVKALEAEGVEYVFGIPGEENLDLLESLRRSKIRLVLTRHEQAAGFMAATYGRLTGRTGVCLATLGPGATNFVT
AAAYAQLGGMPMLMITGQKPIKSSKQGHFQIVDVVGMMQPLTKFTRQIVSIGNIPSAVREAFRRAEEERPGAAHLELPED
IAHEEGDGKPIPRSFSRRPIAEQKAVAHAVDAIQAARHPLLMIGAGGNRKTTCKMLLEFVDKTGIPFFTTQMGKGVIDET
HPLWLGNATLSDGDFVHRAIEHADCIINVGHDVIEKPPFFMRADDKTVIHVNFLGAQVDPVYFPQIEVVGDIANAVWQMK
EAIAPQPHWDFERFAMIKEHFDAHLQKGQHDPRFPMYPVRIVNDLYNALPVDGIVCLDNGMYKIWFARYWRAHEPNSLLL
DNALASMGAGLPSAIATKIVHPQRKVIAVCGDGGFMMNSQELETAVRLGLDIVVMILRDDAFGMIRWKQENMNFPDFAMT
LKNPDFVSYAQSYGAHGHRVESADALEPLLRECFASPGVHVIDVPVDYSDNERVLNREIKRLSAQL
>Mature_546_residues
MKASDLFVKALEAEGVEYVFGIPGEENLDLLESLRRSKIRLVLTRHEQAAGFMAATYGRLTGRTGVCLATLGPGATNFVT
AAAYAQLGGMPMLMITGQKPIKSSKQGHFQIVDVVGMMQPLTKFTRQIVSIGNIPSAVREAFRRAEEERPGAAHLELPED
IAHEEGDGKPIPRSFSRRPIAEQKAVAHAVDAIQAARHPLLMIGAGGNRKTTCKMLLEFVDKTGIPFFTTQMGKGVIDET
HPLWLGNATLSDGDFVHRAIEHADCIINVGHDVIEKPPFFMRADDKTVIHVNFLGAQVDPVYFPQIEVVGDIANAVWQMK
EAIAPQPHWDFERFAMIKEHFDAHLQKGQHDPRFPMYPVRIVNDLYNALPVDGIVCLDNGMYKIWFARYWRAHEPNSLLL
DNALASMGAGLPSAIATKIVHPQRKVIAVCGDGGFMMNSQELETAVRLGLDIVVMILRDDAFGMIRWKQENMNFPDFAMT
LKNPDFVSYAQSYGAHGHRVESADALEPLLRECFASPGVHVIDVPVDYSDNERVLNREIKRLSAQL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=547, Percent_Identity=27.0566727605119, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI21361361, Length=531, Percent_Identity=25.2354048964218, Blast_Score=150, Evalue=3e-36,
Organism=Escherichia coli, GI1790104, Length=543, Percent_Identity=28.9134438305709, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI87081685, Length=554, Percent_Identity=28.3393501805054, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1786717, Length=572, Percent_Identity=24.1258741258741, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1787096, Length=526, Percent_Identity=23.9543726235741, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788716, Length=544, Percent_Identity=24.6323529411765, Blast_Score=122, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17542570, Length=565, Percent_Identity=23.8938053097345, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=565, Percent_Identity=23.3628318584071, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=23.3628318584071, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=567, Percent_Identity=29.8059964726631, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6320816, Length=488, Percent_Identity=22.7459016393443, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321524, Length=488, Percent_Identity=24.3852459016393, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=470, Percent_Identity=24.6808510638298, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=486, Percent_Identity=24.0740740740741, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19922626, Length=482, Percent_Identity=26.3485477178423, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60597; Mature: 60597

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASDLFVKALEAEGVEYVFGIPGEENLDLLESLRRSKIRLVLTRHEQAAGFMAATYGRL
CCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHH
TGRTGVCLATLGPGATNFVTAAAYAQLGGMPMLMITGQKPIKSSKQGHFQIVDVVGMMQP
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHH
LTKFTRQIVSIGNIPSAVREAFRRAEEERPGAAHLELPEDIAHEEGDGKPIPRSFSRRPI
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
AEQKAVAHAVDAIQAARHPLLMIGAGGNRKTTCKMLLEFVDKTGIPFFTTQMGKGVIDET
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCC
HPLWLGNATLSDGDFVHRAIEHADCIINVGHDVIEKPPFFMRADDKTVIHVNFLGAQVDP
CCEEECCCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCEEECCCCEEEEEEEECCCCCC
VYFPQIEVVGDIANAVWQMKEAIAPQPHWDFERFAMIKEHFDAHLQKGQHDPRFPMYPVR
EECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
IVNDLYNALPVDGIVCLDNGMYKIWFARYWRAHEPNSLLLDNALASMGAGLPSAIATKIV
HHHHHHHHCCCCCEEEECCCEEEEHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHC
HPQRKVIAVCGDGGFMMNSQELETAVRLGLDIVVMILRDDAFGMIRWKQENMNFPDFAMT
CCCCEEEEEECCCCEEECHHHHHHHHHHCHHHEEEEEECCCCEEEEEECCCCCCCCEEEE
LKNPDFVSYAQSYGAHGHRVESADALEPLLRECFASPGVHVIDVPVDYSDNERVLNREIK
ECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
RLSAQL
HHHCCC
>Mature Secondary Structure
MKASDLFVKALEAEGVEYVFGIPGEENLDLLESLRRSKIRLVLTRHEQAAGFMAATYGRL
CCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHH
TGRTGVCLATLGPGATNFVTAAAYAQLGGMPMLMITGQKPIKSSKQGHFQIVDVVGMMQP
CCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHH
LTKFTRQIVSIGNIPSAVREAFRRAEEERPGAAHLELPEDIAHEEGDGKPIPRSFSRRPI
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
AEQKAVAHAVDAIQAARHPLLMIGAGGNRKTTCKMLLEFVDKTGIPFFTTQMGKGVIDET
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCC
HPLWLGNATLSDGDFVHRAIEHADCIINVGHDVIEKPPFFMRADDKTVIHVNFLGAQVDP
CCEEECCCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCEEECCCCEEEEEEEECCCCCC
VYFPQIEVVGDIANAVWQMKEAIAPQPHWDFERFAMIKEHFDAHLQKGQHDPRFPMYPVR
EECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
IVNDLYNALPVDGIVCLDNGMYKIWFARYWRAHEPNSLLLDNALASMGAGLPSAIATKIV
HHHHHHHHCCCCCEEEECCCEEEEHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHC
HPQRKVIAVCGDGGFMMNSQELETAVRLGLDIVVMILRDDAFGMIRWKQENMNFPDFAMT
CCCCEEEEEECCCCEEECHHHHHHHHHHCHHHEEEEEECCCCEEEEEECCCCCCCCEEEE
LKNPDFVSYAQSYGAHGHRVESADALEPLLRECFASPGVHVIDVPVDYSDNERVLNREIK
ECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
RLSAQL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]