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The map label for this gene is mmsB [H]

Identifier: 189352310

GI number: 189352310

Start: 359235

End: 360104

Strand: Direct

Name: mmsB [H]

Synonym: BMULJ_03529

Alternate gene names: 189352310

Gene position: 359235-360104 (Clockwise)

Preceding gene: 189352298

Following gene: 189352311

Centisome position: 14.53

GC content: 68.16

Gene sequence:

>870_bases
ATGAAGACGGTAGGCGTGATCGGACTGGGAAACATGGGGCGCGGCATCGCGTCGTCGCTGAAGCGGGCCGGCTTCGATGT
GCTGGGGTTCGATGCGAACGCAGAGAGCGTGCGGCAACTGGCCGACGAAGGCGTGCGGCCCTGCGCGTCGGTTGCGGAGA
TCGCGTCGGCGGTCGACGTGCTGATCCTGTCGCTGCCGACCTCGGCGATCGTCGAAGCGGTCGTGCTCGGCGACGGCGGC
GTCGCCTCGCATGCAAAGCGCGGGCTGATCGTGATCGACACGACGACCGCCGATCCGAACAGCACGCGCAAGGTCGCGGC
CGCGCTCGATGCGCATGGCATCGGCTTCATCGACGGGCCCGTCAGCGGCGGCCCGAAGGGCGCGGCAACCGCGACGATGA
CGATGGTGCTCGGCGGCGCCGACGAGCACATCGCGGCCGTGCAGCCGGTGCTGTCGGCGATCAGCGCGAAACAGGTGCAT
GTCGGCCCCGCGGGCGCGGGCCATGTGACGAAGCTGCTGAACAACCTGCTGACGGGCGTGCATCTGCTCGTCACGAGCGA
AGCGGTCAGAGCCGCGGAGGCGGCGGGCGTCGACAAGGCGCGGCTGATCGAAGCGCTGAGCGGGGGATCCGGACGCAACA
GCGCAACGCTGACGAATTACCCGACGTGGATCCTGAACGAGCGTTTCGATTCGGGCTTCACGATGAAACTGATGCGCAAG
GACATGCGCCTCGCGCTCGAACTGCTGCAGCGCGCACACGTGAGCGCGCCGATCGCAAGCGAAGCGGGCCGCCTGTGGGC
CGCGAGCGCGGAATCGATCGGCGACGCGGAAGACTTCAACCGCATCGTCGAATTCGTCGACGCGCGCTGA

Upstream 100 bases:

>100_bases
ACGGGCCGAAGATCGTCGACAACGGCAGCGGAACCGGCGCGAAACGCAAGCGCGACGCGGCTTCCGCGCGAACCTTCGAA
TGCAACGATCGGGAGCGGCA

Downstream 100 bases:

>100_bases
GCGCCGATCCATCACCACGAGGAAACGATATGGCATCCCCCATCGAACAGGCGCTGCTCGACGCATTTCGACAGTTCTTT
CCCGGCGCACAGACCGTCGG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKTVGVIGLGNMGRGIASSLKRAGFDVLGFDANAESVRQLADEGVRPCASVAEIASAVDVLILSLPTSAIVEAVVLGDGG
VASHAKRGLIVIDTTTADPNSTRKVAAALDAHGIGFIDGPVSGGPKGAATATMTMVLGGADEHIAAVQPVLSAISAKQVH
VGPAGAGHVTKLLNNLLTGVHLLVTSEAVRAAEAAGVDKARLIEALSGGSGRNSATLTNYPTWILNERFDSGFTMKLMRK
DMRLALELLQRAHVSAPIASEAGRLWAASAESIGDAEDFNRIVEFVDAR

Sequences:

>Translated_289_residues
MKTVGVIGLGNMGRGIASSLKRAGFDVLGFDANAESVRQLADEGVRPCASVAEIASAVDVLILSLPTSAIVEAVVLGDGG
VASHAKRGLIVIDTTTADPNSTRKVAAALDAHGIGFIDGPVSGGPKGAATATMTMVLGGADEHIAAVQPVLSAISAKQVH
VGPAGAGHVTKLLNNLLTGVHLLVTSEAVRAAEAAGVDKARLIEALSGGSGRNSATLTNYPTWILNERFDSGFTMKLMRK
DMRLALELLQRAHVSAPIASEAGRLWAASAESIGDAEDFNRIVEFVDAR
>Mature_289_residues
MKTVGVIGLGNMGRGIASSLKRAGFDVLGFDANAESVRQLADEGVRPCASVAEIASAVDVLILSLPTSAIVEAVVLGDGG
VASHAKRGLIVIDTTTADPNSTRKVAAALDAHGIGFIDGPVSGGPKGAATATMTMVLGGADEHIAAVQPVLSAISAKQVH
VGPAGAGHVTKLLNNLLTGVHLLVTSEAVRAAEAAGVDKARLIEALSGGSGRNSATLTNYPTWILNERFDSGFTMKLMRK
DMRLALELLQRAHVSAPIASEAGRLWAASAESIGDAEDFNRIVEFVDAR

Specific function: Catalyzes the conversion of 2-formylglutarate to (S)-2- hydroxymethylglutarate. Has very low activity with (S)-3- hydroxyisobutyrate [H]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=28.719723183391, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI40556376, Length=265, Percent_Identity=25.2830188679245, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1790315, Length=286, Percent_Identity=29.3706293706294, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI145693186, Length=244, Percent_Identity=33.6065573770492, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1786719, Length=285, Percent_Identity=27.3684210526316, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789092, Length=291, Percent_Identity=29.2096219931272, Blast_Score=107, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17557316, Length=297, Percent_Identity=30.976430976431, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24655230, Length=291, Percent_Identity=31.2714776632302, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI19922568, Length=291, Percent_Identity=31.2714776632302, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.291 [H]

Molecular weight: Translated: 29648; Mature: 29648

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVGVIGLGNMGRGIASSLKRAGFDVLGFDANAESVRQLADEGVRPCASVAEIASAVDV
CCCEEEEECCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
LILSLPTSAIVEAVVLGDGGVASHAKRGLIVIDTTTADPNSTRKVAAALDAHGIGFIDGP
HHHHCCHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEECCC
VSGGPKGAATATMTMVLGGADEHIAAVQPVLSAISAKQVHVGPAGAGHVTKLLNNLLTGV
CCCCCCCCHHHEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHH
HLLVTSEAVRAAEAAGVDKARLIEALSGGSGRNSATLTNYPTWILNERFDSGFTMKLMRK
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEECCCCHHEECCCCCCCCHHHHHHH
DMRLALELLQRAHVSAPIASEAGRLWAASAESIGDAEDFNRIVEFVDAR
HHHHHHHHHHHHHCCCCCHHHCCCEEECCHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTVGVIGLGNMGRGIASSLKRAGFDVLGFDANAESVRQLADEGVRPCASVAEIASAVDV
CCCEEEEECCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
LILSLPTSAIVEAVVLGDGGVASHAKRGLIVIDTTTADPNSTRKVAAALDAHGIGFIDGP
HHHHCCHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEECCC
VSGGPKGAATATMTMVLGGADEHIAAVQPVLSAISAKQVHVGPAGAGHVTKLLNNLLTGV
CCCCCCCCHHHEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHH
HLLVTSEAVRAAEAAGVDKARLIEALSGGSGRNSATLTNYPTWILNERFDSGFTMKLMRK
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEECCCCHHEECCCCCCCCHHHHHHH
DMRLALELLQRAHVSAPIASEAGRLWAASAESIGDAEDFNRIVEFVDAR
HHHHHHHHHHHHHCCCCCHHHCCCEEECCHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA