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The map label for this gene is ramA [H]

Identifier: 189352276

GI number: 189352276

Start: 323894

End: 324712

Strand: Direct

Name: ramA [H]

Synonym: BMULJ_03495

Alternate gene names: 189352276

Gene position: 323894-324712 (Clockwise)

Preceding gene: 189352275

Following gene: 189352278

Centisome position: 13.1

GC content: 63.13

Gene sequence:

>819_bases
ATGACCCAACCTGTTGTTCGGCTGTTCCAATCGACGTGCGTGGATGGCGACGTCGCGCACAATCTCGACGCCGCGCTGCG
CGCAATCGCCGACAGTACTGGCCGGGCCGATCTGATCGTGTTCCCGGAAACGTTCATTCCGGGTTTCCCGACGCCGGAAA
ACGTAGCAAAGCTGGCGGAGCCGATCGATGGCACGTCGATCTCGGCGATCCGCGCGGCCGCGAGAGCGGCGCGCGTGAGC
GTCGCGATCGGCTTCGCGGAAAGCGACGGCGGCCACTACTACAATACCGCGCTGCTCATCGATGAGCACGGCCACATACT
GCTGAAATACCGCAAGACACATCTCTATGCGTCGGATATTGGCGTGTTCGAACGCGGGCAAACCTTTCCAGTATGCGAGT
GGCACGGCATTCGCGTCGGGATGCTGATCTGTTTCGATATCGAATTTCCCGAGACCGCGCGGATACTTGCGCGCAACGGT
GCCGAACTGATGCTCGTCCTGGATGGGCTGATGTGCCCGTACGGGAACGTCCATCGCACGATGCTGCCGGTGCGGGCGAT
GGAAAACCAGGCGTATATGGTGCTTGTCAATCGGGCTGGACCGGGCGACCGATATACGTTCGCGGGCCAGAGCCATGTGG
CCGATCCGTTCGGCTGTAGCGTGTTCGTCGCGGATGCCGAGCGCGCGGACCCGCAGGACGTGCGGCTGGACATGAGCGCA
GTGGCGCGCTCGCGGGAAGCGTTCAGCTATGTGGAACTGACCGCCATCACGGTCGGCGAAACGCCGATGCCGCCGCGCGG
CGCTATCAGCGCCGAGTGA

Upstream 100 bases:

>100_bases
TCAGCCGCTCGCTCGATCTTGAGTACGAACAACGCGTGATCGACGCGAGTGACGAAGGCGAACTGATCGCCATCGCGCAC
CGCTCGACCGAGGAGCAATC

Downstream 100 bases:

>100_bases
GGCCGGGCGCTTCCGCGTCTGCAGCGCCGCAGCCGCGTAGCGGATTCCGCGATGCTTCCGGGGATCGGCATACAAGCGCC
GCATCGACATGAAGAAACGC

Product: predicted amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTQPVVRLFQSTCVDGDVAHNLDAALRAIADSTGRADLIVFPETFIPGFPTPENVAKLAEPIDGTSISAIRAAARAARVS
VAIGFAESDGGHYYNTALLIDEHGHILLKYRKTHLYASDIGVFERGQTFPVCEWHGIRVGMLICFDIEFPETARILARNG
AELMLVLDGLMCPYGNVHRTMLPVRAMENQAYMVLVNRAGPGDRYTFAGQSHVADPFGCSVFVADAERADPQDVRLDMSA
VARSREAFSYVELTAITVGETPMPPRGAISAE

Sequences:

>Translated_272_residues
MTQPVVRLFQSTCVDGDVAHNLDAALRAIADSTGRADLIVFPETFIPGFPTPENVAKLAEPIDGTSISAIRAAARAARVS
VAIGFAESDGGHYYNTALLIDEHGHILLKYRKTHLYASDIGVFERGQTFPVCEWHGIRVGMLICFDIEFPETARILARNG
AELMLVLDGLMCPYGNVHRTMLPVRAMENQAYMVLVNRAGPGDRYTFAGQSHVADPFGCSVFVADAERADPQDVRLDMSA
VARSREAFSYVELTAITVGETPMPPRGAISAE
>Mature_271_residues
TQPVVRLFQSTCVDGDVAHNLDAALRAIADSTGRADLIVFPETFIPGFPTPENVAKLAEPIDGTSISAIRAAARAARVSV
AIGFAESDGGHYYNTALLIDEHGHILLKYRKTHLYASDIGVFERGQTFPVCEWHGIRVGMLICFDIEFPETARILARNGA
ELMLVLDGLMCPYGNVHRTMLPVRAMENQAYMVLVNRAGPGDRYTFAGQSHVADPFGCSVFVADAERADPQDVRLDMSAV
ARSREAFSYVELTAITVGETPMPPRGAISAE

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=210, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI297632350, Length=206, Percent_Identity=27.1844660194175, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI297632348, Length=206, Percent_Identity=27.1844660194175, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI5031947, Length=206, Percent_Identity=27.1844660194175, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1786412, Length=246, Percent_Identity=31.3008130081301, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17556280, Length=261, Percent_Identity=29.1187739463602, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323383, Length=199, Percent_Identity=30.6532663316583, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322335, Length=270, Percent_Identity=23.7037037037037, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 29557; Mature: 29426

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPVVRLFQSTCVDGDVAHNLDAALRAIADSTGRADLIVFPETFIPGFPTPENVAKLAE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHCCCCCCHHHHHHHHC
PIDGTSISAIRAAARAARVSVAIGFAESDGGHYYNTALLIDEHGHILLKYRKTHLYASDI
CCCCCCHHHHHHHHHHEEEEEEEEEEECCCCEEEEEEEEEECCCCEEEEEEEEEEEECCC
GVFERGQTFPVCEWHGIRVGMLICFDIEFPETARILARNGAELMLVLDGLMCPYGNVHRT
CCCCCCCCCCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCHHH
MLPVRAMENQAYMVLVNRAGPGDRYTFAGQSHVADPFGCSVFVADAERADPQDVRLDMSA
EEEHEECCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCHHHEEEEHHH
VARSREAFSYVELTAITVGETPMPPRGAISAE
HHHHHHHHEEEEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TQPVVRLFQSTCVDGDVAHNLDAALRAIADSTGRADLIVFPETFIPGFPTPENVAKLAE
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHCCCCCCHHHHHHHHC
PIDGTSISAIRAAARAARVSVAIGFAESDGGHYYNTALLIDEHGHILLKYRKTHLYASDI
CCCCCCHHHHHHHHHHEEEEEEEEEEECCCCEEEEEEEEEECCCCEEEEEEEEEEEECCC
GVFERGQTFPVCEWHGIRVGMLICFDIEFPETARILARNGAELMLVLDGLMCPYGNVHRT
CCCCCCCCCCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCHHH
MLPVRAMENQAYMVLVNRAGPGDRYTFAGQSHVADPFGCSVFVADAERADPQDVRLDMSA
EEEHEECCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCHHHEEEEHHH
VARSREAFSYVELTAITVGETPMPPRGAISAE
HHHHHHHHEEEEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA