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The map label for this gene is shiA [H]

Identifier: 189352220

GI number: 189352220

Start: 259263

End: 260597

Strand: Direct

Name: shiA [H]

Synonym: BMULJ_03438

Alternate gene names: 189352220

Gene position: 259263-260597 (Clockwise)

Preceding gene: 189352216

Following gene: 189352221

Centisome position: 10.48

GC content: 68.16

Gene sequence:

>1335_bases
ATGCACTCACCCGCACCCACCTCGACGGTTACGCTATCCGTCTCCCGCCATGCCGGTTCGGTTCGCCGTGCGGTCACGAC
CGCGGTGGTCGGACAGGCGCTCGAATGGTATGACTTCTTCCTGTACGGCACGGCCGCCGCATTGCTGTTCGGCAAGCTGT
TCTTTCCGGTCGGCACCGATCCGCTGACCGGCACGATCGCGGCGTTCGGCGGCTTCACGGTCGGCTTCGTCGCGCGACCG
ATCGGCGGCCTGCTGTGCGGCCACATCGGCGATCGGTACGGCCGCAAGACCGTGATGATGCTGACGATGTTGACGATGGG
CGTCGCGACCGCCGGGATGGGCTTGTTGCCGACCTATCGCGAGATCGGGATCGCCGCGCCGATCGCGCTCGTGCTGCTGC
GCGTGCTGCAGGGTCTCGCCGCGGGCGGTGAATGGAGCGGCAGCATCCTGTTGATCCACGAGAACGCGCCCGCCGAGCGG
CGCGGTGCGCTCGCCGCGTGGAGCCCGTGCGGCGCCGCGCTCGGCTTCGTGTTGTCGACCGCCGCGTTTCTGCTCGTGCA
GCGCCTGCCTGTCGCCGACATCGAAAGCTGGGGCTGGCGCATCCCGTTTCTCGCGAGCATCGTGCTGGTCGGCTTCGGCC
TGTGGATGCGCCGCTCGGTCGGCGAAAGCGCGGCGTTCGAGCACGTGCGTTCCGAGCAGCACGCGAGCGCGGCACCGCTC
GTCGACGTGCTGCGCGAGTGCCCGCGCGAAGTCGCCACCGTGTTCGGCTTGCGCTTCGGCGAAGGCGCGGTGTCGTACCT
GTTCTTCTCGTTCTCGCTTGCCTACGGCAAGTTCATCGGCCTCGACGCGTCGTGGCTGCTGAGCGGGCTCGTGATCTCGA
TGCTGCTGATGATTCCGGTGACGCTCGCGGCCGGCCGGCTCACCGACCGGATCGGCCGCAAGCCCGTGTACCTGGTCGGC
GCAATCGTCGTCGTGCTGATCGCTTACCCGTATTTCGTGCTGCTCCAGTCGGGCCGGTTCGCGTACGTACTCGCCGCGCT
GATTCTCGCGAACAGCGTCGCGATCGGGATTCTCGAAGGCGCGCAGCCTGCGTTCATCAGCGAGCTGCTACCGGTGCGGC
TGCGGTTTTCGGGCCTCGGCATCGGACGCGAGATTGCGTCGGTGCTCGGCGGCGGCCTGTCGCCGATGATCGCGACCGCA
CTGCTTGCGCACTACCGCAGCGCGGCGCCGATCGTCATCTATCTGATCGTGCTCGGCGCGATCACCATCGTCGCGACCTG
CATCGCGCCGGAAACCTATCCGAAGGCGGCGCGCGACGCGCACCGCGTGCGCTGA

Upstream 100 bases:

>100_bases
GCCGCTCTTATCGTGGGTCGCACGCAGCATCGACTCGATCGCATGGCTGCCGCCGACTGCCGGTTCAGCAGGATCCCTGA
CATTCTTTTCGGAACCTCCG

Downstream 100 bases:

>100_bases
ACGCCGCTCGCCCCTTCGCTTTCCCTTCGTCATTTCAGGATCAGGACATCGCCATGCAAACTTCCACCGTCGTTCGCCCA
TTCGAGCCGGCCGCGCCTTC

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MHSPAPTSTVTLSVSRHAGSVRRAVTTAVVGQALEWYDFFLYGTAAALLFGKLFFPVGTDPLTGTIAAFGGFTVGFVARP
IGGLLCGHIGDRYGRKTVMMLTMLTMGVATAGMGLLPTYREIGIAAPIALVLLRVLQGLAAGGEWSGSILLIHENAPAER
RGALAAWSPCGAALGFVLSTAAFLLVQRLPVADIESWGWRIPFLASIVLVGFGLWMRRSVGESAAFEHVRSEQHASAAPL
VDVLRECPREVATVFGLRFGEGAVSYLFFSFSLAYGKFIGLDASWLLSGLVISMLLMIPVTLAAGRLTDRIGRKPVYLVG
AIVVVLIAYPYFVLLQSGRFAYVLAALILANSVAIGILEGAQPAFISELLPVRLRFSGLGIGREIASVLGGGLSPMIATA
LLAHYRSAAPIVIYLIVLGAITIVATCIAPETYPKAARDAHRVR

Sequences:

>Translated_444_residues
MHSPAPTSTVTLSVSRHAGSVRRAVTTAVVGQALEWYDFFLYGTAAALLFGKLFFPVGTDPLTGTIAAFGGFTVGFVARP
IGGLLCGHIGDRYGRKTVMMLTMLTMGVATAGMGLLPTYREIGIAAPIALVLLRVLQGLAAGGEWSGSILLIHENAPAER
RGALAAWSPCGAALGFVLSTAAFLLVQRLPVADIESWGWRIPFLASIVLVGFGLWMRRSVGESAAFEHVRSEQHASAAPL
VDVLRECPREVATVFGLRFGEGAVSYLFFSFSLAYGKFIGLDASWLLSGLVISMLLMIPVTLAAGRLTDRIGRKPVYLVG
AIVVVLIAYPYFVLLQSGRFAYVLAALILANSVAIGILEGAQPAFISELLPVRLRFSGLGIGREIASVLGGGLSPMIATA
LLAHYRSAAPIVIYLIVLGAITIVATCIAPETYPKAARDAHRVR
>Mature_444_residues
MHSPAPTSTVTLSVSRHAGSVRRAVTTAVVGQALEWYDFFLYGTAAALLFGKLFFPVGTDPLTGTIAAFGGFTVGFVARP
IGGLLCGHIGDRYGRKTVMMLTMLTMGVATAGMGLLPTYREIGIAAPIALVLLRVLQGLAAGGEWSGSILLIHENAPAER
RGALAAWSPCGAALGFVLSTAAFLLVQRLPVADIESWGWRIPFLASIVLVGFGLWMRRSVGESAAFEHVRSEQHASAAPL
VDVLRECPREVATVFGLRFGEGAVSYLFFSFSLAYGKFIGLDASWLLSGLVISMLLMIPVTLAAGRLTDRIGRKPVYLVG
AIVVVLIAYPYFVLLQSGRFAYVLAALILANSVAIGILEGAQPAFISELLPVRLRFSGLGIGREIASVLGGGLSPMIATA
LLAHYRSAAPIVIYLIVLGAITIVATCIAPETYPKAARDAHRVR

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=441, Percent_Identity=39.0022675736961, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1790550, Length=439, Percent_Identity=32.1184510250569, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=35.9154929577465, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1788942, Length=405, Percent_Identity=29.1358024691358, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082231, Length=252, Percent_Identity=27.7777777777778, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87082404, Length=415, Percent_Identity=24.8192771084337, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47027; Mature: 47027

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSPAPTSTVTLSVSRHAGSVRRAVTTAVVGQALEWYDFFLYGTAAALLFGKLFFPVGTD
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLTGTIAAFGGFTVGFVARPIGGLLCGHIGDRYGRKTVMMLTMLTMGVATAGMGLLPTYR
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHH
EIGIAAPIALVLLRVLQGLAAGGEWSGSILLIHENAPAERRGALAAWSPCGAALGFVLST
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHH
AAFLLVQRLPVADIESWGWRIPFLASIVLVGFGLWMRRSVGESAAFEHVRSEQHASAAPL
HHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHH
VDVLRECPREVATVFGLRFGEGAVSYLFFSFSLAYGKFIGLDASWLLSGLVISMLLMIPV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TLAAGRLTDRIGRKPVYLVGAIVVVLIAYPYFVLLQSGRFAYVLAALILANSVAIGILEG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECC
AQPAFISELLPVRLRFSGLGIGREIASVLGGGLSPMIATALLAHYRSAAPIVIYLIVLGA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
ITIVATCIAPETYPKAARDAHRVR
HHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MHSPAPTSTVTLSVSRHAGSVRRAVTTAVVGQALEWYDFFLYGTAAALLFGKLFFPVGTD
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLTGTIAAFGGFTVGFVARPIGGLLCGHIGDRYGRKTVMMLTMLTMGVATAGMGLLPTYR
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHH
EIGIAAPIALVLLRVLQGLAAGGEWSGSILLIHENAPAERRGALAAWSPCGAALGFVLST
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHH
AAFLLVQRLPVADIESWGWRIPFLASIVLVGFGLWMRRSVGESAAFEHVRSEQHASAAPL
HHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHH
VDVLRECPREVATVFGLRFGEGAVSYLFFSFSLAYGKFIGLDASWLLSGLVISMLLMIPV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TLAAGRLTDRIGRKPVYLVGAIVVVLIAYPYFVLLQSGRFAYVLAALILANSVAIGILEG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECC
AQPAFISELLPVRLRFSGLGIGREIASVLGGGLSPMIATALLAHYRSAAPIVIYLIVLGA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
ITIVATCIAPETYPKAARDAHRVR
HHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]