Click here to switch to the map view.

The map label for this gene is dnaK [H]

Identifier: 189352191

GI number: 189352191

Start: 225870

End: 227711

Strand: Direct

Name: dnaK [H]

Synonym: BMULJ_03409

Alternate gene names: 189352191

Gene position: 225870-227711 (Clockwise)

Preceding gene: 189352190

Following gene: 189352192

Centisome position: 9.13

GC content: 70.3

Gene sequence:

>1842_bases
GTGAGCGATCCGCGCTATTCGATCGGTATCGATCTCGGTACCACGCACTGCGCGCTGTCGTATGTCGACAGCGCCGCCAG
CGACGGCGAAAAAATCGCACAGCACGTGCTGCCGATCACGCAACTCACGGCACCCGGCGCGCTCGAATCGCGCGACCTGC
TGCCGTCGTTTCTCTATCTGCCGCATCCGAGCGAACTCACGCAGGGCGACCTGACGCTGCCGTGGACCGCGTCGCGCGAC
TTCGCGGTCGGCGAGATGGCGCGCAGCCGCGGCGCCGGCACGCCGATCCGGCTCGTGTCGAGCGCGAAGAGCTGGCTGTG
CCACCCCGGCGTCGATCGCCGCGCGGCGATCCTGCCGAGCGATGCGCCGCCCGAGGTCACGCGCGTGTCGCCGCTCGAAA
GCTCGATCCGCTATCTGACGCATCTGCGCGAAGCGTGGGATCACGCGCATCCCGACGCGCCGTTCGTCGAGCAGGACGTC
ACCGTCACGATCCCCGCCTCGTTCGACCCGGCGGCGCGTGAACTGACGGCCGAAGCCGCGCGCGCGGCCGGCTATACGCG
GATGACGCTGCTCGAGGAGCCGCAGGCCGCGCTGTATAGCTGGATCCAGAAGAGCGAAGGCGGCTGGCGCAAGCAGGTGA
AGGTCGGCGATCTGATTCTCTGCGTCGACGTCGGCGGCGGCACGACCGACTTGTCGCTGATCGCGGTCGTCGAGCGCGAC
GGCAATCTCGAGCTGCATCGCGTCGCGGTCGGCGAACACATCCTGCTCGGCGGCGACAACATGGATCTCGCGCTCGCGCA
CGTCGTCGCGCGCAAGCTCGCGCAGCAGGGCACGCAGGCCGATCCGTGGCAGCTGCGCGCGCTCACGTACGCGTGCCGCA
GCGCGAAGGAAACGCTGTTGTCCGACCCGACGACCGACGCGGTGCCGCTCGTCGTGCCGAGCCGCGGCTCGAAGCTGATC
GGCGGCTCGATCCGCACCGAGCTCACGCGTGCGGAACTGACACAGACGATACTCGAAGGCTTCTTCCCGCAGGTCGATGC
GGCCGCGCGCCCGATGACGCGCGCGCGCGTCGGCCTCACCCAGCTCGGCCTGCCGTATGCGCAGGACGCGGGCATCACGC
GCCATCTCGCGGCGTTCCTCGGCCGGCAGGTCGCGGCGCTCGACGCGCTCGAAGGCGTGCAGCATACGCTGCCGGCCGGC
GCGACGTTCCTGCATCCGACCGCCGTGCTGTTCAACGGCGGCGTGTTCAAGTCGTCGCTGCTGACGCAGCGCGTGCTCGA
CACGCTGAACGGCTGGCTCGCCGCCGAAGGCGCGCCGCCCGCCCGGCTGCTCGAAGGCGCGGATCTCGATCTCGCCGTCG
CGCGCGGCGCCGCGTACTACGGCTACGTGAAGCGCGGCCGCGGCGTGCGCATTCGCGGCGGTACGGCGCGTGCGTACTAC
GTCGCGATCGAGTCGGCGATGCCGGCCGTGCCGGGGCTCGAACCGCCGGTGCAGGCGCTCTGCGTCGCGCCGTTCGGGAT
GGAGGAAGGCACGAGCGCCGCGCTGCCGCCGCAGGAATTCGGCCTCGTCGTCGGCGAGCCCGTGCATTTCCGCTTCTTCG
GCTCGTCGGTGCGTCGTCAGGACGAAGTCGGCACGCTGCTCGATTTCTGGTCGCCCGACGAACTGCAGGAACTCGAGGAA
ATTCAGGCGACGCTGCCGGCCGAAGGGCGCACCGTCGGCGAGATCGTGCCGGTCAGGCTGCATGCGCGCGTGACCGAGGC
CGGTACGCTCGAGCTCGAAGCGATCCCGAGCGGCACGGACGAGCGCTGGAAGGTCGAGTTCGACGTGCGCGGCACCGCTT
GA

Upstream 100 bases:

>100_bases
GCACCGTCAGCCATCGCGGCTGGCGCGTCGCGGACGTGCGTCTGCCGAAGCTGACGGGCAGCCACGACGCGTCGGTGATC
GCGCCGGCGGAGGTGGAACT

Downstream 100 bases:

>100_bases
GCGTAATGAAGCGCTATACGGTCGGCATCGACCTCGGCACCAGCAATACGGTCGTCGCCTACGTCGAGCCCGGCTCGGAC
GCGATTCGCGTGTTCGACGT

Product: putative molecular chaperone

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MSDPRYSIGIDLGTTHCALSYVDSAASDGEKIAQHVLPITQLTAPGALESRDLLPSFLYLPHPSELTQGDLTLPWTASRD
FAVGEMARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPSDAPPEVTRVSPLESSIRYLTHLREAWDHAHPDAPFVEQDV
TVTIPASFDPAARELTAEAARAAGYTRMTLLEEPQAALYSWIQKSEGGWRKQVKVGDLILCVDVGGGTTDLSLIAVVERD
GNLELHRVAVGEHILLGGDNMDLALAHVVARKLAQQGTQADPWQLRALTYACRSAKETLLSDPTTDAVPLVVPSRGSKLI
GGSIRTELTRAELTQTILEGFFPQVDAAARPMTRARVGLTQLGLPYAQDAGITRHLAAFLGRQVAALDALEGVQHTLPAG
ATFLHPTAVLFNGGVFKSSLLTQRVLDTLNGWLAAEGAPPARLLEGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
VAIESAMPAVPGLEPPVQALCVAPFGMEEGTSAALPPQEFGLVVGEPVHFRFFGSSVRRQDEVGTLLDFWSPDELQELEE
IQATLPAEGRTVGEIVPVRLHARVTEAGTLELEAIPSGTDERWKVEFDVRGTA

Sequences:

>Translated_613_residues
MSDPRYSIGIDLGTTHCALSYVDSAASDGEKIAQHVLPITQLTAPGALESRDLLPSFLYLPHPSELTQGDLTLPWTASRD
FAVGEMARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPSDAPPEVTRVSPLESSIRYLTHLREAWDHAHPDAPFVEQDV
TVTIPASFDPAARELTAEAARAAGYTRMTLLEEPQAALYSWIQKSEGGWRKQVKVGDLILCVDVGGGTTDLSLIAVVERD
GNLELHRVAVGEHILLGGDNMDLALAHVVARKLAQQGTQADPWQLRALTYACRSAKETLLSDPTTDAVPLVVPSRGSKLI
GGSIRTELTRAELTQTILEGFFPQVDAAARPMTRARVGLTQLGLPYAQDAGITRHLAAFLGRQVAALDALEGVQHTLPAG
ATFLHPTAVLFNGGVFKSSLLTQRVLDTLNGWLAAEGAPPARLLEGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
VAIESAMPAVPGLEPPVQALCVAPFGMEEGTSAALPPQEFGLVVGEPVHFRFFGSSVRRQDEVGTLLDFWSPDELQELEE
IQATLPAEGRTVGEIVPVRLHARVTEAGTLELEAIPSGTDERWKVEFDVRGTA
>Mature_612_residues
SDPRYSIGIDLGTTHCALSYVDSAASDGEKIAQHVLPITQLTAPGALESRDLLPSFLYLPHPSELTQGDLTLPWTASRDF
AVGEMARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPSDAPPEVTRVSPLESSIRYLTHLREAWDHAHPDAPFVEQDVT
VTIPASFDPAARELTAEAARAAGYTRMTLLEEPQAALYSWIQKSEGGWRKQVKVGDLILCVDVGGGTTDLSLIAVVERDG
NLELHRVAVGEHILLGGDNMDLALAHVVARKLAQQGTQADPWQLRALTYACRSAKETLLSDPTTDAVPLVVPSRGSKLIG
GSIRTELTRAELTQTILEGFFPQVDAAARPMTRARVGLTQLGLPYAQDAGITRHLAAFLGRQVAALDALEGVQHTLPAGA
TFLHPTAVLFNGGVFKSSLLTQRVLDTLNGWLAAEGAPPARLLEGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYYV
AIESAMPAVPGLEPPVQALCVAPFGMEEGTSAALPPQEFGLVVGEPVHFRFFGSSVRRQDEVGTLLDFWSPDELQELEEI
QATLPAEGRTVGEIVPVRLHARVTEAGTLELEAIPSGTDERWKVEFDVRGTA

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Escherichia coli, GI1786196, Length=383, Percent_Identity=25.3263707571802, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1788875, Length=306, Percent_Identity=29.7385620915033, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17541098, Length=209, Percent_Identity=27.7511961722488, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17534015, Length=383, Percent_Identity=24.0208877284595, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17534013, Length=383, Percent_Identity=24.0208877284595, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507981, Length=203, Percent_Identity=28.0788177339901, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323004, Length=381, Percent_Identity=23.6220472440945, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320950, Length=321, Percent_Identity=24.9221183800623, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319314, Length=381, Percent_Identity=24.1469816272966, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319396, Length=318, Percent_Identity=25.1572327044025, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17738165, Length=258, Percent_Identity=27.1317829457364, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737941, Length=181, Percent_Identity=32.0441988950276, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17647515, Length=236, Percent_Identity=28.8135593220339, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24663992, Length=236, Percent_Identity=28.8135593220339, Blast_Score=66, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24663988, Length=236, Percent_Identity=28.8135593220339, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 65825; Mature: 65694

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00297 HSP70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPRYSIGIDLGTTHCALSYVDSAASDGEKIAQHVLPITQLTAPGALESRDLLPSFLYL
CCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCHHCCCHHEEC
PHPSELTQGDLTLPWTASRDFAVGEMARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPS
CCCCCCCCCCEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEECCC
DAPPEVTRVSPLESSIRYLTHLREAWDHAHPDAPFVEQDVTVTIPASFDPAARELTAEAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
RAAGYTRMTLLEEPQAALYSWIQKSEGGWRKQVKVGDLILCVDVGGGTTDLSLIAVVERD
HHCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCCEEEEEEEECC
GNLELHRVAVGEHILLGGDNMDLALAHVVARKLAQQGTQADPWQLRALTYACRSAKETLL
CCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
SDPTTDAVPLVVPSRGSKLIGGSIRTELTRAELTQTILEGFFPQVDAAARPMTRARVGLT
CCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHCHH
QLGLPYAQDAGITRHLAAFLGRQVAALDALEGVQHTLPAGATFLHPTAVLFNGGVFKSSL
HCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCHHHEECCCHHHHHH
LTQRVLDTLNGWLAAEGAPPARLLEGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
HHHHHHHHHCCEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHCCCCEEEEECCCCEEEE
VAIESAMPAVPGLEPPVQALCVAPFGMEEGTSAALPPQEFGLVVGEPVHFRFFGSSVRRQ
EEEECCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCHHHCCEEECCCEEEEECCCHHHCH
DEVGTLLDFWSPDELQELEEIQATLPAEGRTVGEIVPVRLHARVTEAGTLELEAIPSGTD
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEEECCCCCC
ERWKVEFDVRGTA
CCEEEEEEECCCC
>Mature Secondary Structure 
SDPRYSIGIDLGTTHCALSYVDSAASDGEKIAQHVLPITQLTAPGALESRDLLPSFLYL
CCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCHHCCCHHEEC
PHPSELTQGDLTLPWTASRDFAVGEMARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPS
CCCCCCCCCCEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEECCC
DAPPEVTRVSPLESSIRYLTHLREAWDHAHPDAPFVEQDVTVTIPASFDPAARELTAEAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
RAAGYTRMTLLEEPQAALYSWIQKSEGGWRKQVKVGDLILCVDVGGGTTDLSLIAVVERD
HHCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCCEEEEEEEECC
GNLELHRVAVGEHILLGGDNMDLALAHVVARKLAQQGTQADPWQLRALTYACRSAKETLL
CCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
SDPTTDAVPLVVPSRGSKLIGGSIRTELTRAELTQTILEGFFPQVDAAARPMTRARVGLT
CCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHCHH
QLGLPYAQDAGITRHLAAFLGRQVAALDALEGVQHTLPAGATFLHPTAVLFNGGVFKSSL
HCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCHHHEECCCHHHHHH
LTQRVLDTLNGWLAAEGAPPARLLEGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
HHHHHHHHHCCEEEECCCCHHHHHCCCCCEEEECCCCHHHHHHCCCCEEEEECCCCEEEE
VAIESAMPAVPGLEPPVQALCVAPFGMEEGTSAALPPQEFGLVVGEPVHFRFFGSSVRRQ
EEEECCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCHHHCCEEECCCEEEEECCCHHHCH
DEVGTLLDFWSPDELQELEEIQATLPAEGRTVGEIVPVRLHARVTEAGTLELEAIPSGTD
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEEECCCCCC
ERWKVEFDVRGTA
CCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]