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The map label for this gene is argT [H]

Identifier: 189352160

GI number: 189352160

Start: 188527

End: 189309

Strand: Direct

Name: argT [H]

Synonym: BMULJ_03378

Alternate gene names: 189352160

Gene position: 188527-189309 (Clockwise)

Preceding gene: 189352159

Following gene: 189352161

Centisome position: 7.62

GC content: 66.28

Gene sequence:

>783_bases
ATGAAGAAGTGGACGATGGCGCTCGTGGCGGCGCTGGCGGCGGGCGCCGCGCACGCAGGCGACTGGACGGGCAAGGAGAT
TCGGCTCGCGGTCGATCCGACCTATCCGCCGCTCGAGTACAAGCTGCCGGACGGCACGTTGACCGGCTTCGGCGTCGACA
TCACGAATGCGCTGTGCGCGGAGCTGCGTGCGCGATGCGTGTGGGTCGAGTCGAGCTTCGACGGGATGATTCCGGGGCTG
CTCGCACGCAAGTTCGACGTGATCGCCTCGTCGATGACGATCACGCCGAAGCGGATGCAGCAGATCGCGTTCACGAACCG
GATCTCGAACGCGCCTGCGCGGCTGATCGCGCGCAAGGGCTCGCCGCTGCTGCCGACGGCCGCAGCGTTGAAGGGCAAGC
GCGTCGGCGTCGAACAGGGCTCCGCGCAAGCCGACTACGCGATCGCGAACTGGCAGCCGGCCGGCGCGCAGATCGTGTCG
TACCAGAACCAGGATCAGGTGTATGCCGATCTCGTGACCGGGCGCCTCGATGCCGCGTTCCAGGCGTCGATCGCGGCCAG
CGACGGCTTCCTGAAAAAGCCGCAGGGCAAGGACTTCGCGTTCGTCGGCGCACCGATCGAGGACACGAAGTACTTCGGCC
AGGGCGACGGGCTCGGGCTGCGCAAGCAGGACACCGAACTGCGCGAAGCGTTCAACCGCGCGCTCGCGACGATTCTCGCG
AACGGCACGTATCAGCGAATCAACCGCAAGTACTTCGACTTCGACATCTACGGCGCGAAGTAA

Upstream 100 bases:

>100_bases
ATCGCGCTCGCCGAGCGCTATCTCGCGTGATGTCGCGCACGCGCGGCAGCCGGGCCGCCGATGCGGCCGCACGATTTCGG
TTCATACAAGGAGACTGGCA

Downstream 100 bases:

>100_bases
ACGAACGGGCGTCGCGCGGCCACGACGCGCGGCGCACCGCAACGCCGCAGACGACGCGACGCCTCGCATCGCGCGCGCTC
GCATCGCGTGCGGCACCACC

Product: polar amino acid transport system substrate-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKKWTMALVAALAAGAAHAGDWTGKEIRLAVDPTYPPLEYKLPDGTLTGFGVDITNALCAELRARCVWVESSFDGMIPGL
LARKFDVIASSMTITPKRMQQIAFTNRISNAPARLIARKGSPLLPTAAALKGKRVGVEQGSAQADYAIANWQPAGAQIVS
YQNQDQVYADLVTGRLDAAFQASIAASDGFLKKPQGKDFAFVGAPIEDTKYFGQGDGLGLRKQDTELREAFNRALATILA
NGTYQRINRKYFDFDIYGAK

Sequences:

>Translated_260_residues
MKKWTMALVAALAAGAAHAGDWTGKEIRLAVDPTYPPLEYKLPDGTLTGFGVDITNALCAELRARCVWVESSFDGMIPGL
LARKFDVIASSMTITPKRMQQIAFTNRISNAPARLIARKGSPLLPTAAALKGKRVGVEQGSAQADYAIANWQPAGAQIVS
YQNQDQVYADLVTGRLDAAFQASIAASDGFLKKPQGKDFAFVGAPIEDTKYFGQGDGLGLRKQDTELREAFNRALATILA
NGTYQRINRKYFDFDIYGAK
>Mature_260_residues
MKKWTMALVAALAAGAAHAGDWTGKEIRLAVDPTYPPLEYKLPDGTLTGFGVDITNALCAELRARCVWVESSFDGMIPGL
LARKFDVIASSMTITPKRMQQIAFTNRISNAPARLIARKGSPLLPTAAALKGKRVGVEQGSAQADYAIANWQPAGAQIVS
YQNQDQVYADLVTGRLDAAFQASIAASDGFLKKPQGKDFAFVGAPIEDTKYFGQGDGLGLRKQDTELREAFNRALATILA
NGTYQRINRKYFDFDIYGAK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=261, Percent_Identity=49.4252873563218, Blast_Score=267, Evalue=5e-73,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=51.2820512820513, Blast_Score=266, Evalue=7e-73,
Organism=Escherichia coli, GI1787085, Length=253, Percent_Identity=35.5731225296443, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787088, Length=252, Percent_Identity=32.9365079365079, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1788228, Length=229, Percent_Identity=30.1310043668122, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787031, Length=250, Percent_Identity=29.6, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28171; Mature: 28171

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWTMALVAALAAGAAHAGDWTGKEIRLAVDPTYPPLEYKLPDGTLTGFGVDITNALCA
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEECCHHHHHHHHH
ELRARCVWVESSFDGMIPGLLARKFDVIASSMTITPKRMQQIAFTNRISNAPARLIARKG
HHHHEEEEEECCCCCCCHHHHHHHHHHHHHCEEECHHHHHHHHHHHHCCCCCHHHHCCCC
SPLLPTAAALKGKRVGVEQGSAQADYAIANWQPAGAQIVSYQNQDQVYADLVTGRLDAAF
CCCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHCHHH
QASIAASDGFLKKPQGKDFAFVGAPIEDTKYFGQGDGLGLRKQDTELREAFNRALATILA
HHEEECCCCCCCCCCCCCEEEECCCHHHCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHC
NGTYQRINRKYFDFDIYGAK
CCHHHHHCCEEEEEEEECCC
>Mature Secondary Structure
MKKWTMALVAALAAGAAHAGDWTGKEIRLAVDPTYPPLEYKLPDGTLTGFGVDITNALCA
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEECCHHHHHHHHH
ELRARCVWVESSFDGMIPGLLARKFDVIASSMTITPKRMQQIAFTNRISNAPARLIARKG
HHHHEEEEEECCCCCCCHHHHHHHHHHHHHCEEECHHHHHHHHHHHHCCCCCHHHHCCCC
SPLLPTAAALKGKRVGVEQGSAQADYAIANWQPAGAQIVSYQNQDQVYADLVTGRLDAAF
CCCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHCHHH
QASIAASDGFLKKPQGKDFAFVGAPIEDTKYFGQGDGLGLRKQDTELREAFNRALATILA
HHEEECCCCCCCCCCCCCEEEECCCHHHCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHC
NGTYQRINRKYFDFDIYGAK
CCHHHHHCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]